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The map label for this gene is rsmB [H]

Identifier: 167624219

GI number: 167624219

Start: 2780152

End: 2781366

Strand: Reverse

Name: rsmB [H]

Synonym: Shal_2295

Alternate gene names: 167624219

Gene position: 2781366-2780152 (Counterclockwise)

Preceding gene: 167624220

Following gene: 167624211

Centisome position: 53.21

GC content: 45.27

Gene sequence:

>1215_bases
ATGTTGAAATCTCCGCTTTCTCCTAGCTCAAATGAACTTGTGATCAGTATTCTCGATCTTGTTTTATCAGATGGTAAACC
GTTAGATCGCGCCTATTCTCAGCATTTTTCTGGTCTAAAACTCGAGCCTACTGAACAAGGGCGTATTACCTTTGTCGTTG
GAGATATTCTTAGAAGGTTAAACCTTTACTGCTTCCTTTCAGATGTAAAACCTGAAGAGATGGCGCGGTTAGGCACTCGT
TTATTGAATGTGTGGCACCTCTTTAATGAATTACCTTTGCCGAAAATGCAGTACTCGTTACAAATAGACCCTAAAGTATT
AGCGACTCGTATCGAAGAAGCGAAAGCGCAGCCCGTATTATGGGACGGTTGCCCAGACTGGCTAAATGAGATGGGTCAGA
AGCAGATGGGAGATGCGTGGGCTGCAGAAAGAGCGGCGTTAAACAAACCTGCAAAGCGTTTTTTACGTACCAATATTTTA
AAGTGTACACGTGAAGAACTCGCAAATGCACTGCGCAAAGAGGGCGTGCAAACCGTTAATGTCGATGGCGTAGATACCGC
TTTAGAGGTCACTTCTGATTCTGCACTGTTTAGAACAGAAGCGTTTAAAAAAGGCTGGTTCGAGCAGCAGGATGCGGGTT
CACAGCTAGTTGCGTTAGCACTGGATGCAAAACCTGGAATGCGAGTCGTTGATGCCTGTGCCGGGGCAGGAGGTAAAACT
TTATCAATTTCAGCACAAATGCAAGGTAAGGGCCGTTTACTGGCAATGGATGTCGAGCAGTGGAAACTGGACAATCTTAA
GCAGCGTGCACGTCGCGGCGGCGCTCATAATGTTGAAACCCGCATTATTGCCAGCAGCAAGACCATTAAGCGTCTAAAGT
TGACGGCAGACAGAGTGTTACTCGATGTACCTTGTTCTGGCTTAGGCGTATTGAAGCGTAATCCAGATGCAAAGTGGCGT
GATACTCCTGAGCGTTTACCTGTATTGGTTGAATTGCAAAAGCACATTCTTCAGAGCTATAGTCGTATGGTCAAAGTTGG
CGGCATGTTGGTTTATGCAACGTGCTCGATTATGCCGGAAGAGAACCGTGATCAAGTCGATGCGTTCTTAGCTGAAAATA
AACACTTTAAGTTTATTGAAGATGAAACAATTAGCCCGGCCGAAACGGGTTTTGATGGGTTCTACTTGGCTAAATTAGAG
CGTATTGCAGAATAG

Upstream 100 bases:

>100_bases
ATTAAGCCGCTGCCTTGTGTAGCGGCTTTTTACTATTAGCTTGCGTATTGACTACTTTGCTCCTATGATCGCAGCGTTTT
CTTTTTAGGGTAACTTTTTA

Downstream 100 bases:

>100_bases
TTTTACAGCTTCATATATTAAGTTATTCCGCTAATGACTCGGTCATCGACTCATTAGCGTGATTAACGCTTATCGGTATC
CTAGTGTGAGTTTATCCTGC

Product: Fmu (Sun) domain-containing protein

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MLKSPLSPSSNELVISILDLVLSDGKPLDRAYSQHFSGLKLEPTEQGRITFVVGDILRRLNLYCFLSDVKPEEMARLGTR
LLNVWHLFNELPLPKMQYSLQIDPKVLATRIEEAKAQPVLWDGCPDWLNEMGQKQMGDAWAAERAALNKPAKRFLRTNIL
KCTREELANALRKEGVQTVNVDGVDTALEVTSDSALFRTEAFKKGWFEQQDAGSQLVALALDAKPGMRVVDACAGAGGKT
LSISAQMQGKGRLLAMDVEQWKLDNLKQRARRGGAHNVETRIIASSKTIKRLKLTADRVLLDVPCSGLGVLKRNPDAKWR
DTPERLPVLVELQKHILQSYSRMVKVGGMLVYATCSIMPEENRDQVDAFLAENKHFKFIEDETISPAETGFDGFYLAKLE
RIAE

Sequences:

>Translated_404_residues
MLKSPLSPSSNELVISILDLVLSDGKPLDRAYSQHFSGLKLEPTEQGRITFVVGDILRRLNLYCFLSDVKPEEMARLGTR
LLNVWHLFNELPLPKMQYSLQIDPKVLATRIEEAKAQPVLWDGCPDWLNEMGQKQMGDAWAAERAALNKPAKRFLRTNIL
KCTREELANALRKEGVQTVNVDGVDTALEVTSDSALFRTEAFKKGWFEQQDAGSQLVALALDAKPGMRVVDACAGAGGKT
LSISAQMQGKGRLLAMDVEQWKLDNLKQRARRGGAHNVETRIIASSKTIKRLKLTADRVLLDVPCSGLGVLKRNPDAKWR
DTPERLPVLVELQKHILQSYSRMVKVGGMLVYATCSIMPEENRDQVDAFLAENKHFKFIEDETISPAETGFDGFYLAKLE
RIAE
>Mature_404_residues
MLKSPLSPSSNELVISILDLVLSDGKPLDRAYSQHFSGLKLEPTEQGRITFVVGDILRRLNLYCFLSDVKPEEMARLGTR
LLNVWHLFNELPLPKMQYSLQIDPKVLATRIEEAKAQPVLWDGCPDWLNEMGQKQMGDAWAAERAALNKPAKRFLRTNIL
KCTREELANALRKEGVQTVNVDGVDTALEVTSDSALFRTEAFKKGWFEQQDAGSQLVALALDAKPGMRVVDACAGAGGKT
LSISAQMQGKGRLLAMDVEQWKLDNLKQRARRGGAHNVETRIIASSKTIKRLKLTADRVLLDVPCSGLGVLKRNPDAKWR
DTPERLPVLVELQKHILQSYSRMVKVGGMLVYATCSIMPEENRDQVDAFLAENKHFKFIEDETISPAETGFDGFYLAKLE
RIAE

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=283, Percent_Identity=31.095406360424, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI76150623, Length=283, Percent_Identity=31.095406360424, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI32698918, Length=229, Percent_Identity=29.2576419213974, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI270288818, Length=292, Percent_Identity=26.7123287671233, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI8922322, Length=292, Percent_Identity=26.7123287671233, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI23199998, Length=292, Percent_Identity=26.7123287671233, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI270288816, Length=292, Percent_Identity=26.7123287671233, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI301336155, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI39995082, Length=199, Percent_Identity=27.1356783919598, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI2367212, Length=287, Percent_Identity=31.3588850174216, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081985, Length=224, Percent_Identity=29.9107142857143, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17536757, Length=296, Percent_Identity=29.3918918918919, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115534021, Length=167, Percent_Identity=32.9341317365269, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71998419, Length=167, Percent_Identity=32.9341317365269, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25143471, Length=201, Percent_Identity=30.3482587064677, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=281, Percent_Identity=29.1814946619217, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6319447, Length=175, Percent_Identity=29.7142857142857, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI22024126, Length=281, Percent_Identity=28.1138790035587, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355201, Length=199, Percent_Identity=29.6482412060301, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920494, Length=166, Percent_Identity=34.3373493975904, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 45190; Mature: 45190

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSPLSPSSNELVISILDLVLSDGKPLDRAYSQHFSGLKLEPTEQGRITFVVGDILRRL
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHH
NLYCFLSDVKPEEMARLGTRLLNVWHLFNELPLPKMQYSLQIDPKVLATRIEEAKAQPVL
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCCCEE
WDGCPDWLNEMGQKQMGDAWAAERAALNKPAKRFLRTNILKCTREELANALRKEGVQTVN
ECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VDGVDTALEVTSDSALFRTEAFKKGWFEQQDAGSQLVALALDAKPGMRVVDACAGAGGKT
CCCCCHHEEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCE
LSISAQMQGKGRLLAMDVEQWKLDNLKQRARRGGAHNVETRIIASSKTIKRLKLTADRVL
EEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHEE
LDVPCSGLGVLKRNPDAKWRDTPERLPVLVELQKHILQSYSRMVKVGGMLVYATCSIMPE
EECCCCCCCCEECCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
ENRDQVDAFLAENKHFKFIEDETISPAETGFDGFYLAKLERIAE
CCHHHHHHHHHCCCCEEEECCCCCCCHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLKSPLSPSSNELVISILDLVLSDGKPLDRAYSQHFSGLKLEPTEQGRITFVVGDILRRL
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHH
NLYCFLSDVKPEEMARLGTRLLNVWHLFNELPLPKMQYSLQIDPKVLATRIEEAKAQPVL
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCCCEE
WDGCPDWLNEMGQKQMGDAWAAERAALNKPAKRFLRTNILKCTREELANALRKEGVQTVN
ECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VDGVDTALEVTSDSALFRTEAFKKGWFEQQDAGSQLVALALDAKPGMRVVDACAGAGGKT
CCCCCHHEEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCE
LSISAQMQGKGRLLAMDVEQWKLDNLKQRARRGGAHNVETRIIASSKTIKRLKLTADRVL
EEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHEE
LDVPCSGLGVLKRNPDAKWRDTPERLPVLVELQKHILQSYSRMVKVGGMLVYATCSIMPE
EECCCCCCCCEECCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
ENRDQVDAFLAENKHFKFIEDETISPAETGFDGFYLAKLERIAE
CCHHHHHHHHHCCCCEEEECCCCCCCHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]