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The map label for this gene is hydB [H]

Identifier: 167624186

GI number: 167624186

Start: 2744515

End: 2746218

Strand: Direct

Name: hydB [H]

Synonym: Shal_2262

Alternate gene names: 167624186

Gene position: 2744515-2746218 (Clockwise)

Preceding gene: 167624185

Following gene: 167624187

Centisome position: 52.51

GC content: 45.48

Gene sequence:

>1704_bases
ATGAATAAACGTGTAGTTATCGATCCAATCACTCGTATTGAAGGTCATCTTCGAATTGAAGTCGAAGTCGACCAAAATAA
TGTTATTACCAAAGCTTGGGCATCTTCAACTCTTTGGCGTGGCATTGAAGTGATCCTTAAAGGCCGTACACCGATGGACG
TTGGCCTAATCGTGCAGCGTATTTGTGGCGTGTGTACCTATTCTCACTATCGCTGCGGTACAGAAGCAGTAGAAAATGCC
TTAGGGGTAAAGATCCCGCCTAATGCCAAGTACCTTCGAAGCTTGATGCAATCTTCACTTCATATGCACGATCACATTGT
TCAATTCTATCAACTTCACGGTTTAGATTGGGTCGATGTGGTATCAGCATTGAGCGCCGATCCGGCCTTAGCCGCGCAAG
AAGCAATGAAATATACCGCCAACCCTATTTCTGCAGGGGAAGGCGATCTTCGAGCAGTACAAGAGCGTGTTACAAAACTA
GTTGAAACGGGACAGCTCGGACCTTTTTCCAATGCATATTGGGGCAACCGTACTTATAAATTTACGCCAGAGCAAAACTT
AATCGCGCTATCTCACTATCTAAAAGCGTTAGAGGTTCAACGTGTTGCCGCTGAGATGCTGGCAATATTTGGCGGAAAAT
CACCTCACCCACAATCCATTGTCGTCGGTGGTGTTACCTCTGTTCGCGATATGTTAAGTCCAGCTCGTTTACAAGAGTGG
AAGCAAAAGCATGAATTCGTAAATGATTTCATCATTCGCGCATACAAAGCTGACATAGTCATGGCTGCAGCGGCCTTTGG
CAGCGAGCCAAGCGTACTTGGGGGCTTGAACGTCCCGAATTTCCTAGCAACAGAAGACTTTGTCACTACCGATGGGCAAT
TCATGTTTAACCAAGGGGTGATCATGAACGGCGATCTCGCCAACGTGAGTGATTTAGATCCTATGCAGATCGTAGAAGAT
GTCACCCATGCTTGGTATAAAGCCGATGCACCACAGCACCCTTATGACGGAACGACCATTCCTGACTATACCAGCTTAGT
TGAACGTGAGACCGTTTACGGTAAGCTACCGACTCTTAATGAAGATGGTAAGTACTCATGGGTCAAGTCACCACGTTATA
ACGGTGAACCTATTGAAGTCGGCCCACTCGCAAGCATACTCGTCAGTTATGCTCGTGGTAACCAAGTTGTCGTCGATGCG
GTAAATGGTTTATTGGAAACTACTGGGCTACCGATAGAAGCACTATTCACAACTCTTGGTCGAACAGCCGCTCGCATGTT
GCATACATGTATTGTTGCTGATGAATGTCTTAAGACATTCGATGCCTTGATTACTAACATTCAATCAGATGAATCCACTT
ACACTAAACCTGAAATTGACCCAAATAAAGAGTATTTAGGTCACGCTATGATTGAAGCGCCACGGGGTATGCTCAGCCAC
TGGATTCGGATCAAAAATGGTCTAGTTGAGAACTACCAAGCTGTTGTACCTACAACTTGGAACGCTGGACCTGTGGATGA
GCTGGGGCAAATGGGGCCTTATGAAGCATCACTTATTGGCTTACAGCTTGAAGACCCAACTAAGCCGTTAGAAGTTCTCC
GAGTTATTCACTCTTTTGACCCTTGTATGGCTTGCGCTGTACATGTGATGGATTATAAAGGGCAGAATCTCGGTGAGTTT
AAAATTGATCCCAACGGATTTTAA

Upstream 100 bases:

>100_bases
CCAGTGACACCATAGGTGCCGTCATTCTAGGCGCTACTGCTGTCGGAATTGGCGCACACGCTGTAGCCAGTATTTTTGCC
AAGTCTGAAGAGGAATAGTC

Downstream 100 bases:

>100_bases
TCAAGGGGGATAACATGGCAACTCAATCTGCACCTTACCAAAGGGAACTGATCTTTAGTGCTGCGATAAGAATATTTCAC
TGGCTAAGAGCCTTATCCAT

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MNKRVVIDPITRIEGHLRIEVEVDQNNVITKAWASSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENA
LGVKIPPNAKYLRSLMQSSLHMHDHIVQFYQLHGLDWVDVVSALSADPALAAQEAMKYTANPISAGEGDLRAVQERVTKL
VETGQLGPFSNAYWGNRTYKFTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKSPHPQSIVVGGVTSVRDMLSPARLQEW
KQKHEFVNDFIIRAYKADIVMAAAAFGSEPSVLGGLNVPNFLATEDFVTTDGQFMFNQGVIMNGDLANVSDLDPMQIVED
VTHAWYKADAPQHPYDGTTIPDYTSLVERETVYGKLPTLNEDGKYSWVKSPRYNGEPIEVGPLASILVSYARGNQVVVDA
VNGLLETTGLPIEALFTTLGRTAARMLHTCIVADECLKTFDALITNIQSDESTYTKPEIDPNKEYLGHAMIEAPRGMLSH
WIRIKNGLVENYQAVVPTTWNAGPVDELGQMGPYEASLIGLQLEDPTKPLEVLRVIHSFDPCMACAVHVMDYKGQNLGEF
KIDPNGF

Sequences:

>Translated_567_residues
MNKRVVIDPITRIEGHLRIEVEVDQNNVITKAWASSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENA
LGVKIPPNAKYLRSLMQSSLHMHDHIVQFYQLHGLDWVDVVSALSADPALAAQEAMKYTANPISAGEGDLRAVQERVTKL
VETGQLGPFSNAYWGNRTYKFTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKSPHPQSIVVGGVTSVRDMLSPARLQEW
KQKHEFVNDFIIRAYKADIVMAAAAFGSEPSVLGGLNVPNFLATEDFVTTDGQFMFNQGVIMNGDLANVSDLDPMQIVED
VTHAWYKADAPQHPYDGTTIPDYTSLVERETVYGKLPTLNEDGKYSWVKSPRYNGEPIEVGPLASILVSYARGNQVVVDA
VNGLLETTGLPIEALFTTLGRTAARMLHTCIVADECLKTFDALITNIQSDESTYTKPEIDPNKEYLGHAMIEAPRGMLSH
WIRIKNGLVENYQAVVPTTWNAGPVDELGQMGPYEASLIGLQLEDPTKPLEVLRVIHSFDPCMACAVHVMDYKGQNLGEF
KIDPNGF
>Mature_567_residues
MNKRVVIDPITRIEGHLRIEVEVDQNNVITKAWASSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENA
LGVKIPPNAKYLRSLMQSSLHMHDHIVQFYQLHGLDWVDVVSALSADPALAAQEAMKYTANPISAGEGDLRAVQERVTKL
VETGQLGPFSNAYWGNRTYKFTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKSPHPQSIVVGGVTSVRDMLSPARLQEW
KQKHEFVNDFIIRAYKADIVMAAAAFGSEPSVLGGLNVPNFLATEDFVTTDGQFMFNQGVIMNGDLANVSDLDPMQIVED
VTHAWYKADAPQHPYDGTTIPDYTSLVERETVYGKLPTLNEDGKYSWVKSPRYNGEPIEVGPLASILVSYARGNQVVVDA
VNGLLETTGLPIEALFTTLGRTAARMLHTCIVADECLKTFDALITNIQSDESTYTKPEIDPNKEYLGHAMIEAPRGMLSH
WIRIKNGLVENYQAVVPTTWNAGPVDELGQMGPYEASLIGLQLEDPTKPLEVLRVIHSFDPCMACAVHVMDYKGQNLGEF
KIDPNGF

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=584, Percent_Identity=44.5205479452055, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1787207, Length=579, Percent_Identity=40.9326424870466, Blast_Score=426, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 62702; Mature: 62702

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRVVIDPITRIEGHLRIEVEVDQNNVITKAWASSTLWRGIEVILKGRTPMDVGLIVQR
CCCCEEECCHHHCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHEECCCCCCHHHHHHHH
ICGVCTYSHYRCGTEAVENALGVKIPPNAKYLRSLMQSSLHMHDHIVQFYQLHGLDWVDV
HHHHHCCHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VSALSADPALAAQEAMKYTANPISAGEGDLRAVQERVTKLVETGQLGPFSNAYWGNRTYK
HHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
FTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKSPHPQSIVVGGVTSVRDMLSPARLQEW
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCHHHHHHH
KQKHEFVNDFIIRAYKADIVMAAAAFGSEPSVLGGLNVPNFLATEDFVTTDGQFMFNQGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEECCCCEEEECCE
IMNGDLANVSDLDPMQIVEDVTHAWYKADAPQHPYDGTTIPDYTSLVERETVYGKLPTLN
EECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
EDGKYSWVKSPRYNGEPIEVGPLASILVSYARGNQVVVDAVNGLLETTGLPIEALFTTLG
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCHHHHHHHHH
RTAARMLHTCIVADECLKTFDALITNIQSDESTYTKPEIDPNKEYLGHAMIEAPRGMLSH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
WIRIKNGLVENYQAVVPTTWNAGPVDELGQMGPYEASLIGLQLEDPTKPLEVLRVIHSFD
HHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHCCC
PCMACAVHVMDYKGQNLGEFKIDPNGF
HHHHHHHHHHHCCCCCCCEEEECCCCC
>Mature Secondary Structure
MNKRVVIDPITRIEGHLRIEVEVDQNNVITKAWASSTLWRGIEVILKGRTPMDVGLIVQR
CCCCEEECCHHHCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHEECCCCCCHHHHHHHH
ICGVCTYSHYRCGTEAVENALGVKIPPNAKYLRSLMQSSLHMHDHIVQFYQLHGLDWVDV
HHHHHCCHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VSALSADPALAAQEAMKYTANPISAGEGDLRAVQERVTKLVETGQLGPFSNAYWGNRTYK
HHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
FTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKSPHPQSIVVGGVTSVRDMLSPARLQEW
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCHHHHHHH
KQKHEFVNDFIIRAYKADIVMAAAAFGSEPSVLGGLNVPNFLATEDFVTTDGQFMFNQGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEECCCCEEEECCE
IMNGDLANVSDLDPMQIVEDVTHAWYKADAPQHPYDGTTIPDYTSLVERETVYGKLPTLN
EECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
EDGKYSWVKSPRYNGEPIEVGPLASILVSYARGNQVVVDAVNGLLETTGLPIEALFTTLG
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCHHHHHHHHH
RTAARMLHTCIVADECLKTFDALITNIQSDESTYTKPEIDPNKEYLGHAMIEAPRGMLSH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
WIRIKNGLVENYQAVVPTTWNAGPVDELGQMGPYEASLIGLQLEDPTKPLEVLRVIHSFD
HHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHCCC
PCMACAVHVMDYKGQNLGEFKIDPNGF
HHHHHHHHHHHCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]