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The map label for this gene is hydA [H]

Identifier: 167624185

GI number: 167624185

Start: 2743376

End: 2744512

Strand: Direct

Name: hydA [H]

Synonym: Shal_2261

Alternate gene names: 167624185

Gene position: 2743376-2744512 (Clockwise)

Preceding gene: 167624184

Following gene: 167624186

Centisome position: 52.49

GC content: 46.7

Gene sequence:

>1137_bases
ATGGAAACACATGAAGCCCTTTACCAGCAAGGTTTAGCGCGAATAGAGCAACTTCGTAAGCAAAAACCTCGCCACAGTGA
AACGCTGCAGCAAAAATTGGAACAAAATGGCATTACTCGCCGTGACTTTATGAAATGGAGCGCATCTGTAACGGCAATGC
TTGCCTTACCTCTTCCGTTTAGCACCTTAGTTGCCGAAGCAGCAGAGCTTGCAGACAGAGTTCCCCTCGTTTGGCTACAT
ATGGCCGAGTGTACAGGATGTACAGAGTCTCTTATTAGAACAACCACACCTAATATTTCCACCTTAATCTTTGATTACGT
CTCACTCGAGTATCAAGAAACTCTAATGGCCGCAGCGGGCTGGCAAGCAGAGGAAAACCTTGAGCGCGCATTATCAGCCT
ATAAAGGTAGTTATCTGCTCGCTATCGAAGGGGCAGTACCAACAGCAAACAACGGTAATTTTTTAACGGTAGGTTGTAAA
GGTCACACTGGACTACATATTGTTAAAGAAGCAGCCAAAGATGCAGCAGCAATTCTTTCTGTCGGCACCTGCGCCGCTTT
CGGTGGTATTCAAGCCGCAGCACCAAATCCAACAGGAGCTGTTGGTGTATCCGAAGTCATTGATAAGCCTGTTATTAACC
TCGGTGGCTGTCCACCTAATGAAACCAATATTGTCGGAACACTGATGTACTTCATCATGTTTGGTAAGTTACCTGCACTG
GATATGTTTAACCGTCCTAAGTGGGCATATGGTGCTCGCGTTCATGATAACTGCGAACGTCGTGGTCACTTCGATGCCGG
TGAGTTTGTAGAAGAGTTTGGTGATCAAGCTGCAAAAGATGGTTATTGCCTTTATAAGTTAGGCTGTAAAGGACCTTATA
CCTACAATAACTGTCCGACAGAACGGTTCAATCACCACACTAGCTGGCCAGTACTTGCTGGTCATGGCTGTATTGGCTGC
TCAGAGCCCGACTTCTGGGACGAGATGGCAGATTTCGAAAAACCTTTAGGAAGGAAATTACTGCATGGTCTTGATGCGAC
CAGTGACACCATAGGTGCCGTCATTCTAGGCGCTACTGCTGTCGGAATTGGCGCACACGCTGTAGCCAGTATTTTTGCCA
AGTCTGAAGAGGAATAG

Upstream 100 bases:

>100_bases
AGGTCTGTAAAGCTTGACGCATATCAATAATACCCAGCGTTTTGAGGCCTAGACTGTTTTCGTTGAACACATTCCTTAAT
AAACAAAAAAAGGTTTAACC

Downstream 100 bases:

>100_bases
TCATGAATAAACGTGTAGTTATCGATCCAATCACTCGTATTGAAGGTCATCTTCGAATTGAAGTCGAAGTCGACCAAAAT
AATGTTATTACCAAAGCTTG

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
METHEALYQQGLARIEQLRKQKPRHSETLQQKLEQNGITRRDFMKWSASVTAMLALPLPFSTLVAEAAELADRVPLVWLH
MAECTGCTESLIRTTTPNISTLIFDYVSLEYQETLMAAAGWQAEENLERALSAYKGSYLLAIEGAVPTANNGNFLTVGCK
GHTGLHIVKEAAKDAAAILSVGTCAAFGGIQAAAPNPTGAVGVSEVIDKPVINLGGCPPNETNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGHFDAGEFVEEFGDQAAKDGYCLYKLGCKGPYTYNNCPTERFNHHTSWPVLAGHGCIGC
SEPDFWDEMADFEKPLGRKLLHGLDATSDTIGAVILGATAVGIGAHAVASIFAKSEEE

Sequences:

>Translated_378_residues
METHEALYQQGLARIEQLRKQKPRHSETLQQKLEQNGITRRDFMKWSASVTAMLALPLPFSTLVAEAAELADRVPLVWLH
MAECTGCTESLIRTTTPNISTLIFDYVSLEYQETLMAAAGWQAEENLERALSAYKGSYLLAIEGAVPTANNGNFLTVGCK
GHTGLHIVKEAAKDAAAILSVGTCAAFGGIQAAAPNPTGAVGVSEVIDKPVINLGGCPPNETNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGHFDAGEFVEEFGDQAAKDGYCLYKLGCKGPYTYNNCPTERFNHHTSWPVLAGHGCIGC
SEPDFWDEMADFEKPLGRKLLHGLDATSDTIGAVILGATAVGIGAHAVASIFAKSEEE
>Mature_378_residues
METHEALYQQGLARIEQLRKQKPRHSETLQQKLEQNGITRRDFMKWSASVTAMLALPLPFSTLVAEAAELADRVPLVWLH
MAECTGCTESLIRTTTPNISTLIFDYVSLEYQETLMAAAGWQAEENLERALSAYKGSYLLAIEGAVPTANNGNFLTVGCK
GHTGLHIVKEAAKDAAAILSVGTCAAFGGIQAAAPNPTGAVGVSEVIDKPVINLGGCPPNETNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGHFDAGEFVEEFGDQAAKDGYCLYKLGCKGPYTYNNCPTERFNHHTSWPVLAGHGCIGC
SEPDFWDEMADFEKPLGRKLLHGLDATSDTIGAVILGATAVGIGAHAVASIFAKSEEE

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=334, Percent_Identity=44.0119760479042, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1789371, Length=292, Percent_Identity=47.6027397260274, Blast_Score=271, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 40972; Mature: 40972

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METHEALYQQGLARIEQLRKQKPRHSETLQQKLEQNGITRRDFMKWSASVTAMLALPLPF
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCH
STLVAEAAELADRVPLVWLHMAECTGCTESLIRTTTPNISTLIFDYVSLEYQETLMAAAG
HHHHHHHHHHHHCCCCCEEEHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
WQAEENLERALSAYKGSYLLAIEGAVPTANNGNFLTVGCKGHTGLHIVKEAAKDAAAILS
CCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VGTCAAFGGIQAAAPNPTGAVGVSEVIDKPVINLGGCPPNETNIVGTLMYFIMFGKLPAL
HHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCH
DMFNRPKWAYGARVHDNCERRGHFDAGEFVEEFGDQAAKDGYCLYKLGCKGPYTYNNCPT
HHCCCCCCCCCCCHHHCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCH
ERFNHHTSWPVLAGHGCIGCSEPDFWDEMADFEKPLGRKLLHGLDATSDTIGAVILGATA
HHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VGIGAHAVASIFAKSEEE
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METHEALYQQGLARIEQLRKQKPRHSETLQQKLEQNGITRRDFMKWSASVTAMLALPLPF
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCH
STLVAEAAELADRVPLVWLHMAECTGCTESLIRTTTPNISTLIFDYVSLEYQETLMAAAG
HHHHHHHHHHHHCCCCCEEEHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
WQAEENLERALSAYKGSYLLAIEGAVPTANNGNFLTVGCKGHTGLHIVKEAAKDAAAILS
CCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VGTCAAFGGIQAAAPNPTGAVGVSEVIDKPVINLGGCPPNETNIVGTLMYFIMFGKLPAL
HHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCH
DMFNRPKWAYGARVHDNCERRGHFDAGEFVEEFGDQAAKDGYCLYKLGCKGPYTYNNCPT
HHCCCCCCCCCCCHHHCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCH
ERFNHHTSWPVLAGHGCIGCSEPDFWDEMADFEKPLGRKLLHGLDATSDTIGAVILGATA
HHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VGIGAHAVASIFAKSEEE
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]