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The map label for this gene is bkdA1 [H]

Identifier: 167624158

GI number: 167624158

Start: 2709119

End: 2710297

Strand: Reverse

Name: bkdA1 [H]

Synonym: Shal_2234

Alternate gene names: 167624158

Gene position: 2710297-2709119 (Counterclockwise)

Preceding gene: 167624159

Following gene: 167624157

Centisome position: 51.85

GC content: 46.06

Gene sequence:

>1179_bases
ATGAGCAACGCAACAACGAATGCAGAAACAATCCATCGCGTGAGCTTTCTTGATAAAGACTCACTGTCGATCCCGATTTT
AAAAGTATTACAAGCTGACGGTACCGCCTATGAAGGTGCCGTATTACCCGTAATAGACGAAAATTTAGCAAAAAAAATCC
ATGATACTTGTGTGTTTACCCGAGTGTTAGACGAGCGTATGTTGGGGGCTCAGCGTCAAGGCCGTATTAGCTTCTATATG
ACCTGTACTGGTGAAGAGGCTTCTATTATCGGCAGCACCGCGGCTCTTGATGATGGTGATGTGATCTTGGCGCAATATCG
CGAACATGCAGCAATACGCTATCGCGGTTTTACCACTGAACAGTTTATGAACCAGATGTTCAGTAACGAAAAAGATTTTG
GTAAAGGCCGTCAAATGCCGATCCATTACGGTAGCCAAGAACTTAACTATCAAACGATTTCATCACCGCTTGCGACTCAA
ATCCCACAAGCAACTGGTGTCGCCTATGCCCTTAAAAGGCAGGGCAAGCGCAACATTGCCATCTGTTATTTTGGTGAAGG
TGCTGCCTCTGAAGGCGACTTCCATGCAGGCCTGAACATGGCTGCCGTTTTGAATTCTCCGGTTATTTTCTTCTGCCGTA
ACAATGGGTATGCGATCTCGACCCCAACGGAAGAGCAGTTTAAAGGTAATGGTATTGCAAGCCGTGGTGTGGGTTACGGT
ATGCATACTATCCGTGTTGATGGTAACGATATGCTGGCAGTACTCGCTGCAACGCAGCAGGCTCGTGCATATGCGCTAGA
GCATAACGCCCCTGTACTGATTGAAGCCATGACTTATCGCCTAGGCGCGCACTCTTCATCAGACGACCCGTCGGGTTATC
GCTCAAAAGATGAAGAGGCTAAGTGGCAGCAACACGATCCGGTTAAGCGCTTCAAACTGTGGATGATCAATAAAGGTTGG
ATGAACGAGCAAGAAGATGCCGATCTTTATGTGAAATACCGTGAAGAAGTGTTATCTGAGCTCAAAGTGGCAGAGAAAGT
ACCAACTCCTCATCTAGATAGTATCATCGAAGATGTCTACGATACGCCAACACCAATGCTTAAGAAACAGTTAGCACAGT
TGAAAGAACACGTAAAAAAATATCCAGAATCCTATCCAAAAAGCGCAGGGAGAGTATAA

Upstream 100 bases:

>100_bases
GAGAGTTAGTTTACGTTAACGTAATGTTCTTCCCGCGTATTAAAATAGCAAGGCGTCGTATTTAGTCGTTTAGCGGCACC
ATCAATAACAAGAGCACATT

Downstream 100 bases:

>100_bases
GCCGTGGCTCAGATGAATATGTTGCAAGCCATTAACCAAGCGCTGAGCTCGGAAATGGAATCAGATGAAACAATGACAGT
GTTTGGCGAAGATGTTGGAC

Product: 3-methyl-2-oxobutanoate dehydrogenase

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSNATTNAETIHRVSFLDKDSLSIPILKVLQADGTAYEGAVLPVIDENLAKKIHDTCVFTRVLDERMLGAQRQGRISFYM
TCTGEEASIIGSTAALDDGDVILAQYREHAAIRYRGFTTEQFMNQMFSNEKDFGKGRQMPIHYGSQELNYQTISSPLATQ
IPQATGVAYALKRQGKRNIAICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTEEQFKGNGIASRGVGYG
MHTIRVDGNDMLAVLAATQQARAYALEHNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEAKWQQHDPVKRFKLWMINKGW
MNEQEDADLYVKYREEVLSELKVAEKVPTPHLDSIIEDVYDTPTPMLKKQLAQLKEHVKKYPESYPKSAGRV

Sequences:

>Translated_392_residues
MSNATTNAETIHRVSFLDKDSLSIPILKVLQADGTAYEGAVLPVIDENLAKKIHDTCVFTRVLDERMLGAQRQGRISFYM
TCTGEEASIIGSTAALDDGDVILAQYREHAAIRYRGFTTEQFMNQMFSNEKDFGKGRQMPIHYGSQELNYQTISSPLATQ
IPQATGVAYALKRQGKRNIAICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTEEQFKGNGIASRGVGYG
MHTIRVDGNDMLAVLAATQQARAYALEHNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEAKWQQHDPVKRFKLWMINKGW
MNEQEDADLYVKYREEVLSELKVAEKVPTPHLDSIIEDVYDTPTPMLKKQLAQLKEHVKKYPESYPKSAGRV
>Mature_391_residues
SNATTNAETIHRVSFLDKDSLSIPILKVLQADGTAYEGAVLPVIDENLAKKIHDTCVFTRVLDERMLGAQRQGRISFYMT
CTGEEASIIGSTAALDDGDVILAQYREHAAIRYRGFTTEQFMNQMFSNEKDFGKGRQMPIHYGSQELNYQTISSPLATQI
PQATGVAYALKRQGKRNIAICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTEEQFKGNGIASRGVGYGM
HTIRVDGNDMLAVLAATQQARAYALEHNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEAKWQQHDPVKRFKLWMINKGWM
NEQEDADLYVKYREEVLSELKVAEKVPTPHLDSIIEDVYDTPTPMLKKQLAQLKEHVKKYPESYPKSAGRV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=382, Percent_Identity=48.1675392670157, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI258645172, Length=382, Percent_Identity=48.1675392670157, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI291084742, Length=290, Percent_Identity=29.3103448275862, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI4885543, Length=286, Percent_Identity=28.3216783216783, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI4505685, Length=290, Percent_Identity=29.3103448275862, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI291084744, Length=283, Percent_Identity=28.9752650176678, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI291084757, Length=291, Percent_Identity=25.7731958762887, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI86563355, Length=369, Percent_Identity=51.7615176151762, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI86563357, Length=369, Percent_Identity=51.7615176151762, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32564172, Length=283, Percent_Identity=30.0353356890459, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17536047, Length=283, Percent_Identity=30.0353356890459, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321026, Length=314, Percent_Identity=28.343949044586, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355903, Length=371, Percent_Identity=46.900269541779, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24639746, Length=300, Percent_Identity=29.3333333333333, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24639740, Length=300, Percent_Identity=29.3333333333333, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24639744, Length=300, Percent_Identity=29.3333333333333, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28571106, Length=300, Percent_Identity=29.3333333333333, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24639748, Length=278, Percent_Identity=29.4964028776978, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 43722; Mature: 43591

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNATTNAETIHRVSFLDKDSLSIPILKVLQADGTAYEGAVLPVIDENLAKKIHDTCVFT
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCEECCEEEEHHHHHHHHHHHHHHHHH
RVLDERMLGAQRQGRISFYMTCTGEEASIIGSTAALDDGDVILAQYREHAAIRYRGFTTE
HHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEEEHHCCCEEEECCCCHH
QFMNQMFSNEKDFGKGRQMPIHYGSQELNYQTISSPLATQIPQATGVAYALKRQGKRNIA
HHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHCCCHHHHCCCHHHHHHHHHHCCCCCEE
ICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTEEQFKGNGIASRGVGYG
EEEECCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEECCCCHHHHCCCCCCCCCCCCC
MHTIRVDGNDMLAVLAATQQARAYALEHNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEA
EEEEEECCCCEEEEEEHHHHHHHEEECCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCC
KWQQHDPVKRFKLWMINKGWMNEQEDADLYVKYREEVLSELKVAEKVPTPHLDSIIEDVY
CCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DTPTPMLKKQLAQLKEHVKKYPESYPKSAGRV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SNATTNAETIHRVSFLDKDSLSIPILKVLQADGTAYEGAVLPVIDENLAKKIHDTCVFT
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCEECCEEEEHHHHHHHHHHHHHHHHH
RVLDERMLGAQRQGRISFYMTCTGEEASIIGSTAALDDGDVILAQYREHAAIRYRGFTTE
HHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEEEHHCCCEEEECCCCHH
QFMNQMFSNEKDFGKGRQMPIHYGSQELNYQTISSPLATQIPQATGVAYALKRQGKRNIA
HHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHCCCHHHHCCCHHHHHHHHHHCCCCCEE
ICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTEEQFKGNGIASRGVGYG
EEEECCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEECCCCHHHHCCCCCCCCCCCCC
MHTIRVDGNDMLAVLAATQQARAYALEHNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEA
EEEEEECCCCEEEEEEHHHHHHHEEECCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCC
KWQQHDPVKRFKLWMINKGWMNEQEDADLYVKYREEVLSELKVAEKVPTPHLDSIIEDVY
CCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DTPTPMLKKQLAQLKEHVKKYPESYPKSAGRV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]