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The map label for this gene is pdhC [H]

Identifier: 167624156

GI number: 167624156

Start: 2706488

End: 2708128

Strand: Reverse

Name: pdhC [H]

Synonym: Shal_2232

Alternate gene names: 167624156

Gene position: 2708128-2706488 (Counterclockwise)

Preceding gene: 167624157

Following gene: 167624155

Centisome position: 51.81

GC content: 45.7

Gene sequence:

>1641_bases
ATGATTAAAGATTTTATCCTTCCGGATATCGGCGAAGGCGTTGTCGAGTGTGAGCTGGTCGAATGGTTAGTACAAGAGGG
TGATACTGTTGTTGAAGATCAACCTATTGCCGATGTGATGACTGATAAAGCATTAGTACAAATTCCAGCGCCTAATGCTG
GAGTCATCACCAAACTTCACTATGCAAAAGGCGAAATCGCTAAGGTGCATGCACCGCTTTATTCTGTTGATATAACAGAA
GAGGGACAAGCTGCACAAAACGATACCGCTGTGGCTTCGGTAAGTGGTGCTGGCATGACTGCCGAAAGTGAAAGCGCACA
GGCAACTAATACAGAAACTAATACAGAAACTAATACAGTAACGATAGGCTCTGGTGTCACGATTGAAGAGTTTTTGCTGC
CCGATATAGGCGAAGGCATTGTCGAATGTGAACTGGTCGAATGGCTAGTTAACGAAGGTGACATGGTTGTTGAAGATCAG
CCTATTGCCGATGTGATGACCGATAAAGCCTTAGTACAAATTCCCGCGATTAAAAGCGGCAAAATTGCTAAGTTACATTA
TCGAAAAGGTCAGTTGGCCCGTGTACATGAGCCACTATTTGCCATTGAAGTTGTATCCGATGTGGTCAGTGTCAATGCAT
CAGAGCTAAGTGACACCAATACCACTGTCGCTAATGCATCTGTAACGACTAATGAATTTGTACCACAAGGTAAAGCGTTA
GCAAGCCCTGCGGTACGACGATTGGCCCGTAGTCTAGATATTGATATCTCAACAGTTGTTGGCTCTGGTAAAAATGGCCG
AGTGTACAAAGAAGATGTTGAGCGTCACCAGACTGGCGGCGCAGCTGCGCTTTCAACTGCTTCTAACTCAGTAAGCACGC
CTGAGCCAGCAGCACAACCCACTGCTGCTGTTGAAAATACACAAGTTAAAGGTCAAGCTGATAGAGTAGAGCCTATAAAG
GGCGTCCGCGCGGTGATGGCGAAGATGATGATGGAGTCGGTATCGACGATCCCACATTTTACCTATTGTGAAGAGTTTGA
TCTGACCGACTTGGTTGCTCTGCGTGAAAGCATGAAGAAAAAGTATTCATCAGATGAGTTAAAGCTCACCATGATGCCTT
TCTTTATGAAGTCTATGTCACTGGCACTGGCTCAATTTCCAGACATGAACAGCCGAGTTAATGCAGACTGTAGTGAGCAG
ACATATCTAGCAAGCCATAACATAGGTATGGCCGTTGATTCTAAAGTTGGCCTACTGGTGCCTAACGTCAAAGATGTACA
GGATAAAACGATTCTTGAAGTCGCAGCTGAGATCACGCGTTTAACCAATGCTGCGCGAAGTGGCAGGGTATCACCAGCGG
ATCTTAAAGGTGGCAGTATCTCTATTTCTAATATCGGCGCATTAGGTGGCACGGTGGCAACACCCATCATCAATAAGCCT
GAAGTCGCGATCGTTGCCTTAGGTAAACTGCAAGTGTTACCACGCTTTAATGAAAAGGGTGAAGTTGAAGCGCGTAAGAT
CATGCAGGTTAGCTGGTCAGGGGATCATCGCGTTATTGATGGTGGCACCATCGCTCGTTTTTGCAACTTGTGGAAACTCT
ATTTAGAGCAGCCTCAAGAGATGTTGTTAGCCATGCAATAG

Upstream 100 bases:

>100_bases
CGATACACCGTATCCTTTGATCCATGAAAAAGAATATATGCCAGATGCGTTAAAGACGTTTGAAGCGATCAAAGCTTCGG
TTAAATTTTAGGAGCCAGCC

Downstream 100 bases:

>100_bases
GCTACAAGCATTTTGTAAAATGGCGCTAGTGATAGCGCCTTTTTTATTTCTATTATCCTCGTAGTAGTTTTAAATTTGTG
AACAATTCCGTATAATTCCG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MIKDFILPDIGEGVVECELVEWLVQEGDTVVEDQPIADVMTDKALVQIPAPNAGVITKLHYAKGEIAKVHAPLYSVDITE
EGQAAQNDTAVASVSGAGMTAESESAQATNTETNTETNTVTIGSGVTIEEFLLPDIGEGIVECELVEWLVNEGDMVVEDQ
PIADVMTDKALVQIPAIKSGKIAKLHYRKGQLARVHEPLFAIEVVSDVVSVNASELSDTNTTVANASVTTNEFVPQGKAL
ASPAVRRLARSLDIDISTVVGSGKNGRVYKEDVERHQTGGAAALSTASNSVSTPEPAAQPTAAVENTQVKGQADRVEPIK
GVRAVMAKMMMESVSTIPHFTYCEEFDLTDLVALRESMKKKYSSDELKLTMMPFFMKSMSLALAQFPDMNSRVNADCSEQ
TYLASHNIGMAVDSKVGLLVPNVKDVQDKTILEVAAEITRLTNAARSGRVSPADLKGGSISISNIGALGGTVATPIINKP
EVAIVALGKLQVLPRFNEKGEVEARKIMQVSWSGDHRVIDGGTIARFCNLWKLYLEQPQEMLLAMQ

Sequences:

>Translated_546_residues
MIKDFILPDIGEGVVECELVEWLVQEGDTVVEDQPIADVMTDKALVQIPAPNAGVITKLHYAKGEIAKVHAPLYSVDITE
EGQAAQNDTAVASVSGAGMTAESESAQATNTETNTETNTVTIGSGVTIEEFLLPDIGEGIVECELVEWLVNEGDMVVEDQ
PIADVMTDKALVQIPAIKSGKIAKLHYRKGQLARVHEPLFAIEVVSDVVSVNASELSDTNTTVANASVTTNEFVPQGKAL
ASPAVRRLARSLDIDISTVVGSGKNGRVYKEDVERHQTGGAAALSTASNSVSTPEPAAQPTAAVENTQVKGQADRVEPIK
GVRAVMAKMMMESVSTIPHFTYCEEFDLTDLVALRESMKKKYSSDELKLTMMPFFMKSMSLALAQFPDMNSRVNADCSEQ
TYLASHNIGMAVDSKVGLLVPNVKDVQDKTILEVAAEITRLTNAARSGRVSPADLKGGSISISNIGALGGTVATPIINKP
EVAIVALGKLQVLPRFNEKGEVEARKIMQVSWSGDHRVIDGGTIARFCNLWKLYLEQPQEMLLAMQ
>Mature_546_residues
MIKDFILPDIGEGVVECELVEWLVQEGDTVVEDQPIADVMTDKALVQIPAPNAGVITKLHYAKGEIAKVHAPLYSVDITE
EGQAAQNDTAVASVSGAGMTAESESAQATNTETNTETNTVTIGSGVTIEEFLLPDIGEGIVECELVEWLVNEGDMVVEDQ
PIADVMTDKALVQIPAIKSGKIAKLHYRKGQLARVHEPLFAIEVVSDVVSVNASELSDTNTTVANASVTTNEFVPQGKAL
ASPAVRRLARSLDIDISTVVGSGKNGRVYKEDVERHQTGGAAALSTASNSVSTPEPAAQPTAAVENTQVKGQADRVEPIK
GVRAVMAKMMMESVSTIPHFTYCEEFDLTDLVALRESMKKKYSSDELKLTMMPFFMKSMSLALAQFPDMNSRVNADCSEQ
TYLASHNIGMAVDSKVGLLVPNVKDVQDKTILEVAAEITRLTNAARSGRVSPADLKGGSISISNIGALGGTVATPIINKP
EVAIVALGKLQVLPRFNEKGEVEARKIMQVSWSGDHRVIDGGTIARFCNLWKLYLEQPQEMLLAMQ

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=45.4976303317536, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI31711992, Length=567, Percent_Identity=25.0440917107584, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI203098816, Length=482, Percent_Identity=28.0082987551867, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=28.1879194630872, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI19923748, Length=252, Percent_Identity=30.1587301587302, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=34.375, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1786305, Length=541, Percent_Identity=28.2809611829945, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=26.8235294117647, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=38.0281690140845, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI17560088, Length=451, Percent_Identity=26.8292682926829, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=27.8125, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=418, Percent_Identity=27.511961722488, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324258, Length=445, Percent_Identity=25.1685393258427, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18859875, Length=425, Percent_Identity=40.2352941176471, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=28.9592760180996, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 58621; Mature: 58621

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDFILPDIGEGVVECELVEWLVQEGDTVVEDQPIADVMTDKALVQIPAPNAGVITKLH
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHCCCEEEEECCCCCCEEEEEE
YAKGEIAKVHAPLYSVDITEEGQAAQNDTAVASVSGAGMTAESESAQATNTETNTETNTV
ECCCCEEEEECCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
TIGSGVTIEEFLLPDIGEGIVECELVEWLVNEGDMVVEDQPIADVMTDKALVQIPAIKSG
EECCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCEEEECCCCCC
KIAKLHYRKGQLARVHEPLFAIEVVSDVVSVNASELSDTNTTVANASVTTNEFVPQGKAL
CEEEEEECCCCHHHHHCHHHHHHHHHHHHHCCHHHCCCCCCEEEECEEECHHCCCCCHHH
ASPAVRRLARSLDIDISTVVGSGKNGRVYKEDVERHQTGGAAALSTASNSVSTPEPAAQP
HHHHHHHHHHHCCCEEEEEECCCCCCCEEHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
TAAVENTQVKGQADRVEPIKGVRAVMAKMMMESVSTIPHFTYCEEFDLTDLVALRESMKK
CHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
KYSSDELKLTMMPFFMKSMSLALAQFPDMNSRVNADCSEQTYLASHNIGMAVDSKVGLLV
HCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEECCCCCEEC
PNVKDVQDKTILEVAAEITRLTNAARSGRVSPADLKGGSISISNIGALGGTVATPIINKP
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHCCCCCCC
EVAIVALGKLQVLPRFNEKGEVEARKIMQVSWSGDHRVIDGGTIARFCNLWKLYLEQPQE
CEEEEEECCEEECCCCCCCCCCCEEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCHH
MLLAMQ
HHHCCC
>Mature Secondary Structure
MIKDFILPDIGEGVVECELVEWLVQEGDTVVEDQPIADVMTDKALVQIPAPNAGVITKLH
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHCCCEEEEECCCCCCEEEEEE
YAKGEIAKVHAPLYSVDITEEGQAAQNDTAVASVSGAGMTAESESAQATNTETNTETNTV
ECCCCEEEEECCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
TIGSGVTIEEFLLPDIGEGIVECELVEWLVNEGDMVVEDQPIADVMTDKALVQIPAIKSG
EECCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCEEEECCCCCC
KIAKLHYRKGQLARVHEPLFAIEVVSDVVSVNASELSDTNTTVANASVTTNEFVPQGKAL
CEEEEEECCCCHHHHHCHHHHHHHHHHHHHCCHHHCCCCCCEEEECEEECHHCCCCCHHH
ASPAVRRLARSLDIDISTVVGSGKNGRVYKEDVERHQTGGAAALSTASNSVSTPEPAAQP
HHHHHHHHHHHCCCEEEEEECCCCCCCEEHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
TAAVENTQVKGQADRVEPIKGVRAVMAKMMMESVSTIPHFTYCEEFDLTDLVALRESMKK
CHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
KYSSDELKLTMMPFFMKSMSLALAQFPDMNSRVNADCSEQTYLASHNIGMAVDSKVGLLV
HCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEECCCCCEEC
PNVKDVQDKTILEVAAEITRLTNAARSGRVSPADLKGGSISISNIGALGGTVATPIINKP
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHCCCCCCC
EVAIVALGKLQVLPRFNEKGEVEARKIMQVSWSGDHRVIDGGTIARFCNLWKLYLEQPQE
CEEEEEECCEEECCCCCCCCCCCEEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCHH
MLLAMQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]