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The map label for this gene is ycdT [H]

Identifier: 167624095

GI number: 167624095

Start: 2623416

End: 2624720

Strand: Direct

Name: ycdT [H]

Synonym: Shal_2171

Alternate gene names: 167624095

Gene position: 2623416-2624720 (Clockwise)

Preceding gene: 167624092

Following gene: 167624096

Centisome position: 50.19

GC content: 32.87

Gene sequence:

>1305_bases
ATGACAAAAAGGAATGCGTTCAGCATTTGCTTATCTTTAATTGTTTCTATATGTATAGTTAATTTATTCTTGACTGTTAA
TAAAAATAAGTTAAATAGTAAGTTTTTAACTAACATCCTTTTTATTAGTGATGCAATATACCGTTTTAATGTAAACAGCC
TTAACAGTAAGTACGTTTCTCTACCATCAAATGATTTAAAACAGGGGGTCATAGTAAAAAATTCTGAAGCTAAAAAAATT
CGAGACCTATCTGAGCATATAGATAATCTATTTGCTGTATTGAAAATCAACCCAATCATATCTAGCAACTTATGGACGGT
CGCTAATGTATACCCAGAGTATGTCATTCCAAGGCCATTTAGAGAGGAGTACATAAACCTTTTTGATAAGGATTTTGCTG
AGGAAAACTACCTAGAACGTATTTTAAAATTAGAAAACATTGAAAAAACACTAGAAGGAAGTGGTTTAACTGTATTTGAA
ACAGTGAGAGTTTTCGGTCCTTATATAGAAGAGGGAACCAATGAAGAGTTATTTTCAATTTATTACCCACTCTATTTAGA
AGGTGTTGTTTTTTCCGTTTTAGTTATTGATGTAAAAAGTAATTTTTTAAATGATTTCATAGTTGATTTTAATGATGAAG
AATTTACTTGGTTTGAAATGTCAGATGAAGACCGTCAGAGCATAAGTACGATTATATACGAATCCTTAGCACTTAAGACG
GAAGACCGAAAAGTGGGGCTAACAATAAAAACGTCTTTACTGTACAACAGTTTAGCAGTTTTGATATGTACACTCGTCAT
ACACGGCTGTTTTTTAACTGCTGTATACATTAGACGGTTTTATGTCGAAAGCAGCATCGATAGGCTATCTGGATTCTATC
GTAAAGATGGATTTATGAGTAAAAAGATGTCTGTTAGCTCTATATGCTTGATTGATATCGATCATTTTAAGAAAATTAAT
GATGTTTATGGACACATTGTCGGTGACATTGTGATTTCGGAAGTGGCTAACAGAATAAAATCCTCAATGCGATCCACTGA
TCTTGCTATTCGATGGGGCGGCGAAGAGTTTGTTGTTATTTTTAATGGTGAACTGTCTTTCGAGCAATTTAGTAATAAAG
CCAATGGTTTATTGAGCATAATAAATAATGAAACCATCAATGATATAGCCGTCACTATATCTATAGGTGCTATTTACTCT
CAGTCTGAAATGAAATTGCAGGAAGCATTTCATCTTTCTGACGAAGCTCTTTATGAGTCGAAGAACTCTGGACGAAATAA
AGTCACTATTTATCGTAATAGCTGA

Upstream 100 bases:

>100_bases
TTTATGTAAGCTTATTATTAGTGAATAATGAGAAGCATTTGTTAATCAATGAGTTGTGGTTGTGACTCTAGTCTATTACT
ATTGCCGGGTGTTAAATAAC

Downstream 100 bases:

>100_bases
ATGCATATGCAATCAAGTTATCACCATCGCATTGAACAAGCATGATTTAAGTAAACTAATTGACCAGGCAACTCTAATCA
AGCTCCTAATTTGAAGTGGT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTKRNAFSICLSLIVSICIVNLFLTVNKNKLNSKFLTNILFISDAIYRFNVNSLNSKYVSLPSNDLKQGVIVKNSEAKKI
RDLSEHIDNLFAVLKINPIISSNLWTVANVYPEYVIPRPFREEYINLFDKDFAEENYLERILKLENIEKTLEGSGLTVFE
TVRVFGPYIEEGTNEELFSIYYPLYLEGVVFSVLVIDVKSNFLNDFIVDFNDEEFTWFEMSDEDRQSISTIIYESLALKT
EDRKVGLTIKTSLLYNSLAVLICTLVIHGCFLTAVYIRRFYVESSIDRLSGFYRKDGFMSKKMSVSSICLIDIDHFKKIN
DVYGHIVGDIVISEVANRIKSSMRSTDLAIRWGGEEFVVIFNGELSFEQFSNKANGLLSIINNETINDIAVTISIGAIYS
QSEMKLQEAFHLSDEALYESKNSGRNKVTIYRNS

Sequences:

>Translated_434_residues
MTKRNAFSICLSLIVSICIVNLFLTVNKNKLNSKFLTNILFISDAIYRFNVNSLNSKYVSLPSNDLKQGVIVKNSEAKKI
RDLSEHIDNLFAVLKINPIISSNLWTVANVYPEYVIPRPFREEYINLFDKDFAEENYLERILKLENIEKTLEGSGLTVFE
TVRVFGPYIEEGTNEELFSIYYPLYLEGVVFSVLVIDVKSNFLNDFIVDFNDEEFTWFEMSDEDRQSISTIIYESLALKT
EDRKVGLTIKTSLLYNSLAVLICTLVIHGCFLTAVYIRRFYVESSIDRLSGFYRKDGFMSKKMSVSSICLIDIDHFKKIN
DVYGHIVGDIVISEVANRIKSSMRSTDLAIRWGGEEFVVIFNGELSFEQFSNKANGLLSIINNETINDIAVTISIGAIYS
QSEMKLQEAFHLSDEALYESKNSGRNKVTIYRNS
>Mature_433_residues
TKRNAFSICLSLIVSICIVNLFLTVNKNKLNSKFLTNILFISDAIYRFNVNSLNSKYVSLPSNDLKQGVIVKNSEAKKIR
DLSEHIDNLFAVLKINPIISSNLWTVANVYPEYVIPRPFREEYINLFDKDFAEENYLERILKLENIEKTLEGSGLTVFET
VRVFGPYIEEGTNEELFSIYYPLYLEGVVFSVLVIDVKSNFLNDFIVDFNDEEFTWFEMSDEDRQSISTIIYESLALKTE
DRKVGLTIKTSLLYNSLAVLICTLVIHGCFLTAVYIRRFYVESSIDRLSGFYRKDGFMSKKMSVSSICLIDIDHFKKIND
VYGHIVGDIVISEVANRIKSSMRSTDLAIRWGGEEFVVIFNGELSFEQFSNKANGLLSIINNETINDIAVTISIGAIYSQ
SEMKLQEAFHLSDEALYESKNSGRNKVTIYRNS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=138, Percent_Identity=41.304347826087, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=123, Percent_Identity=38.2113821138211, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693134, Length=148, Percent_Identity=31.0810810810811, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=206, Percent_Identity=29.126213592233, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=28.2352941176471, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1787816, Length=125, Percent_Identity=32, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=123, Percent_Identity=34.9593495934959, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=32.5714285714286, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081974, Length=323, Percent_Identity=26.9349845201238, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1788381, Length=131, Percent_Identity=30.5343511450382, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=32.9479768786127, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49652; Mature: 49521

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRNAFSICLSLIVSICIVNLFLTVNKNKLNSKFLTNILFISDAIYRFNVNSLNSKYVS
CCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHEEEECCCCCCCEEE
LPSNDLKQGVIVKNSEAKKIRDLSEHIDNLFAVLKINPIISSNLWTVANVYPEYVIPRPF
CCCCHHCCCEEEECCCHHHHHHHHHHHHHHEEHEEECCHHCCCCEEEECCCCCCCCCCCH
REEYINLFDKDFAEENYLERILKLENIEKTLEGSGLTVFETVRVFGPYIEEGTNEELFSI
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHCCCCCCHHHH
YYPLYLEGVVFSVLVIDVKSNFLNDFIVDFNDEEFTWFEMSDEDRQSISTIIYESLALKT
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHHHHHHHHEEC
EDRKVGLTIKTSLLYNSLAVLICTLVIHGCFLTAVYIRRFYVESSIDRLSGFYRKDGFMS
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KKMSVSSICLIDIDHFKKINDVYGHIVGDIVISEVANRIKSSMRSTDLAIRWGGEEFVVI
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEE
FNGELSFEQFSNKANGLLSIINNETINDIAVTISIGAIYSQSEMKLQEAFHLSDEALYES
ECCCCCHHHHCCCCCCEEEECCCCCCCEEEEEEEECCEECHHHHHHHHHHCCCHHHHHHC
KNSGRNKVTIYRNS
CCCCCCEEEEEECC
>Mature Secondary Structure 
TKRNAFSICLSLIVSICIVNLFLTVNKNKLNSKFLTNILFISDAIYRFNVNSLNSKYVS
CCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHEEEECCCCCCCEEE
LPSNDLKQGVIVKNSEAKKIRDLSEHIDNLFAVLKINPIISSNLWTVANVYPEYVIPRPF
CCCCHHCCCEEEECCCHHHHHHHHHHHHHHEEHEEECCHHCCCCEEEECCCCCCCCCCCH
REEYINLFDKDFAEENYLERILKLENIEKTLEGSGLTVFETVRVFGPYIEEGTNEELFSI
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHCCCCCCHHHH
YYPLYLEGVVFSVLVIDVKSNFLNDFIVDFNDEEFTWFEMSDEDRQSISTIIYESLALKT
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHHHHHHHHEEC
EDRKVGLTIKTSLLYNSLAVLICTLVIHGCFLTAVYIRRFYVESSIDRLSGFYRKDGFMS
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KKMSVSSICLIDIDHFKKINDVYGHIVGDIVISEVANRIKSSMRSTDLAIRWGGEEFVVI
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEE
FNGELSFEQFSNKANGLLSIINNETINDIAVTISIGAIYSQSEMKLQEAFHLSDEALYES
ECCCCCHHHHCCCCCCEEEECCCCCCCEEEEEEEECCEECHHHHHHHHHHCCCHHHHHHC
KNSGRNKVTIYRNS
CCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]