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The map label for this gene is oprM [H]

Identifier: 167624092

GI number: 167624092

Start: 2619613

End: 2621151

Strand: Direct

Name: oprM [H]

Synonym: Shal_2168

Alternate gene names: 167624092

Gene position: 2619613-2621151 (Clockwise)

Preceding gene: 167624091

Following gene: 167624095

Centisome position: 50.12

GC content: 45.81

Gene sequence:

>1539_bases
ATGCCTAATCAACTTAACATCACTCAAGCCTATACCAATCATAAGATGACAATCGGTGGTGTTAACTCGTTTCGAATTAG
CACCACAGCATTTTGTGTCGCCTGTGGGATTTTGCTACTTTCTGGTTGTGCCATGGGGCCAGATTACCAGAGACCAGATC
TTGAAATACCGGAACAATTTCACCACGAGTTGCAGCAAACCGACGAGCAGAACGTTGGCCTCAAGCATTGGCGTGAGTTT
TATCTCGATCCTGAGCTACAGGTATTGATAGAACATGCTCTTGATCAAAACCTCGATTTAGAGGGAGTGCGCTCAAGACT
GTTAGCGGCAAGAGCGGGGGTAACAGTTACCGACGCTGCACTATTTCCCGTAGTAGGACTTGATGCAAATGGCGAGCGAG
CATTAGACAGCGCGTTGACCAGTACCGATCCGGTTAAAGAAGATACTTTTTATGTTGCGGGTACTGTTTCGTGGGAACTG
GATCTTTGGGGAGCGAATCGTCGCAGGAGTGAAGCAGAATTTGCCAATTTCATCTCTTCACAAGAAGCACTTAATTTAGC
CACCATCAGTTTAATCAGTGATGTCGCCAGTCGTTATTATGAATGGCTCGATATTGAGCAACGTTATCAAATATCGCTTA
ATACGGTCGAGCTTCGTAAAAAGGAGCGAGATCTTGCCAGACTGCGAAAACAAAATGGTGTGATATCGGGCCTGCAAGTT
AGGCAGGCTGAGGTGGAGTATCAAAGTGCCAGAGTCACATTGCCAGATCTTGATTATGAGCGAAGTGAAAAGGCCAATCA
GTTGCGGATTTTACTGGGGGAATTTAAATATCCCCTTATCCCTAAAACTGAAGCGCAGTTAAAAGCTGAAGGAGCGTTGT
TTCCTGACTCATTCGCAGTGGGGGTTCCATCACAGATGTTGTCACAGCGCCCAGATGTAAAATCCGCAGAGCAAGCTCTT
GTTGCAGCCAATGCCAATGTCGGTGTCGCTAAAACAGCATTCTTTCCTAAATTTACCATTAGCGGCAGCTATGGTTCTGA
ATCTGAAAGCCTCAGTGACATATTTGATAGCCAAGGGATCACTTGGTCGCTTTTAGGTGGTATCACCGCCCCCATTTTCA
ATGCAGGTAAGATCACTGCTCAATATGATATTGCCCAGGAAGATGCAAAACAGGCGATGCTCAGCTATAGAAGTACAGTG
CTCGGTGCATACTTTGAAGTGAATGATGCGATGAACAGTTTCAGACGCTCTGAATTGGCGATTGACGCTCAGCAGGAATT
AGTTGTGGCATCAACTGAATATACGCGCTTAGCAACGTTAAGATACCGAAATGGGGTTTCGAGTTCATTGGATTTGATGG
ATGCTCAGCGAAGTCTGTTTAGCGCCGAACTGACTCTGAGTCAGGTGAAACGTGACCAGCTACTGTCGATGATTACACTT
TATCGTGCGCTGGGTGGTGGAGTACTGACCAGTGATGAGATGAAGACTGAACTTGAAAAAACGACTGGCGAGCAACAAGT
CGAAGAACTTACTAAATAA

Upstream 100 bases:

>100_bases
ACCATTGGCATCATAATGGTACCGCTGTTTTTTGTGACAACGGCGGGCTGGGTTCGCTCTAAAATCACCGGCAAAGCCGA
TACAGAGGAGGTATCGACTC

Downstream 100 bases:

>100_bases
TCAAATAAATAGACAATTAAATAGAGAGTTAAATCGTTCGAGTATTGATGAGGCTAAAATCAAAATTTATGGGCGATTAG
TCCTCCAATTTCGGGAGGCT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MPNQLNITQAYTNHKMTIGGVNSFRISTTAFCVACGILLLSGCAMGPDYQRPDLEIPEQFHHELQQTDEQNVGLKHWREF
YLDPELQVLIEHALDQNLDLEGVRSRLLAARAGVTVTDAALFPVVGLDANGERALDSALTSTDPVKEDTFYVAGTVSWEL
DLWGANRRRSEAEFANFISSQEALNLATISLISDVASRYYEWLDIEQRYQISLNTVELRKKERDLARLRKQNGVISGLQV
RQAEVEYQSARVTLPDLDYERSEKANQLRILLGEFKYPLIPKTEAQLKAEGALFPDSFAVGVPSQMLSQRPDVKSAEQAL
VAANANVGVAKTAFFPKFTISGSYGSESESLSDIFDSQGITWSLLGGITAPIFNAGKITAQYDIAQEDAKQAMLSYRSTV
LGAYFEVNDAMNSFRRSELAIDAQQELVVASTEYTRLATLRYRNGVSSSLDLMDAQRSLFSAELTLSQVKRDQLLSMITL
YRALGGGVLTSDEMKTELEKTTGEQQVEELTK

Sequences:

>Translated_512_residues
MPNQLNITQAYTNHKMTIGGVNSFRISTTAFCVACGILLLSGCAMGPDYQRPDLEIPEQFHHELQQTDEQNVGLKHWREF
YLDPELQVLIEHALDQNLDLEGVRSRLLAARAGVTVTDAALFPVVGLDANGERALDSALTSTDPVKEDTFYVAGTVSWEL
DLWGANRRRSEAEFANFISSQEALNLATISLISDVASRYYEWLDIEQRYQISLNTVELRKKERDLARLRKQNGVISGLQV
RQAEVEYQSARVTLPDLDYERSEKANQLRILLGEFKYPLIPKTEAQLKAEGALFPDSFAVGVPSQMLSQRPDVKSAEQAL
VAANANVGVAKTAFFPKFTISGSYGSESESLSDIFDSQGITWSLLGGITAPIFNAGKITAQYDIAQEDAKQAMLSYRSTV
LGAYFEVNDAMNSFRRSELAIDAQQELVVASTEYTRLATLRYRNGVSSSLDLMDAQRSLFSAELTLSQVKRDQLLSMITL
YRALGGGVLTSDEMKTELEKTTGEQQVEELTK
>Mature_511_residues
PNQLNITQAYTNHKMTIGGVNSFRISTTAFCVACGILLLSGCAMGPDYQRPDLEIPEQFHHELQQTDEQNVGLKHWREFY
LDPELQVLIEHALDQNLDLEGVRSRLLAARAGVTVTDAALFPVVGLDANGERALDSALTSTDPVKEDTFYVAGTVSWELD
LWGANRRRSEAEFANFISSQEALNLATISLISDVASRYYEWLDIEQRYQISLNTVELRKKERDLARLRKQNGVISGLQVR
QAEVEYQSARVTLPDLDYERSEKANQLRILLGEFKYPLIPKTEAQLKAEGALFPDSFAVGVPSQMLSQRPDVKSAEQALV
AANANVGVAKTAFFPKFTISGSYGSESESLSDIFDSQGITWSLLGGITAPIFNAGKITAQYDIAQEDAKQAMLSYRSTVL
GAYFEVNDAMNSFRRSELAIDAQQELVVASTEYTRLATLRYRNGVSSSLDLMDAQRSLFSAELTLSQVKRDQLLSMITLY
RALGGGVLTSDEMKTELEKTTGEQQVEELTK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=467, Percent_Identity=33.6188436830835, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI2367348, Length=499, Percent_Identity=23.8476953907816, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 56880; Mature: 56748

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNQLNITQAYTNHKMTIGGVNSFRISTTAFCVACGILLLSGCAMGPDYQRPDLEIPEQF
CCCCCEEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
HHELQQTDEQNVGLKHWREFYLDPELQVLIEHALDQNLDLEGVRSRLLAARAGVTVTDAA
HHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEECHH
LFPVVGLDANGERALDSALTSTDPVKEDTFYVAGTVSWELDLWGANRRRSEAEFANFISS
HHCEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHH
QEALNLATISLISDVASRYYEWLDIEQRYQISLNTVELRKKERDLARLRKQNGVISGLQV
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHH
RQAEVEYQSARVTLPDLDYERSEKANQLRILLGEFKYPLIPKTEAQLKAEGALFPDSFAV
HHHHHHHHHCEEECCCCCCCHHCCCCHHEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCC
GVPSQMLSQRPDVKSAEQALVAANANVGVAKTAFFPKFTISGSYGSESESLSDIFDSQGI
CCCHHHHHCCCCCCHHHHHHHEECCCCCEEEHCCCCEEEECCCCCCCHHHHHHHHHCCCC
TWSLLGGITAPIFNAGKITAQYDIAQEDAKQAMLSYRSTVLGAYFEVNDAMNSFRRSELA
EEEECCCCHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHE
IDAQQELVVASTEYTRLATLRYRNGVSSSLDLMDAQRSLFSAELTLSQVKRDQLLSMITL
ECCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRALGGGVLTSDEMKTELEKTTGEQQVEELTK
HHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
PNQLNITQAYTNHKMTIGGVNSFRISTTAFCVACGILLLSGCAMGPDYQRPDLEIPEQF
CCCCEEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
HHELQQTDEQNVGLKHWREFYLDPELQVLIEHALDQNLDLEGVRSRLLAARAGVTVTDAA
HHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEECHH
LFPVVGLDANGERALDSALTSTDPVKEDTFYVAGTVSWELDLWGANRRRSEAEFANFISS
HHCEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHH
QEALNLATISLISDVASRYYEWLDIEQRYQISLNTVELRKKERDLARLRKQNGVISGLQV
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHH
RQAEVEYQSARVTLPDLDYERSEKANQLRILLGEFKYPLIPKTEAQLKAEGALFPDSFAV
HHHHHHHHHCEEECCCCCCCHHCCCCHHEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCC
GVPSQMLSQRPDVKSAEQALVAANANVGVAKTAFFPKFTISGSYGSESESLSDIFDSQGI
CCCHHHHHCCCCCCHHHHHHHEECCCCCEEEHCCCCEEEECCCCCCCHHHHHHHHHCCCC
TWSLLGGITAPIFNAGKITAQYDIAQEDAKQAMLSYRSTVLGAYFEVNDAMNSFRRSELA
EEEECCCCHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHE
IDAQQELVVASTEYTRLATLRYRNGVSSSLDLMDAQRSLFSAELTLSQVKRDQLLSMITL
ECCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRALGGGVLTSDEMKTELEKTTGEQQVEELTK
HHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]