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The map label for this gene is paaF [C]

Identifier: 167624066

GI number: 167624066

Start: 2581189

End: 2582037

Strand: Direct

Name: paaF [C]

Synonym: Shal_2142

Alternate gene names: 167624066

Gene position: 2581189-2582037 (Clockwise)

Preceding gene: 167624065

Following gene: 167624067

Centisome position: 49.38

GC content: 43.23

Gene sequence:

>849_bases
ATGTCAGTTATTTTAGAAAAAGCACAGAAACTGTTTTTATCAGACAACGATGCCAAGGTGCAAACTGGACATGAGTTTGA
AACGGTTAACTATTTAGTTAACGGCAAAATCGGCATTATCGAACTTAATCGACCCGACAAGTTGAATGCATTCAACCAAA
CGATGCGTAGCGAGCTGCAAGCCGCCATAGAGATGGCAGATAATAATGATGATTTAAGAGTCATTATTATTCGTGGTGCT
GGTGATCATTTCTCTTCAGGTGCGGACTTGAAAGAGCTAGTTAGTGCAAAACACGGTATAGAGGCACAGCTACTCAATGA
ATATAAGCCGTTTTTGTCACGCATAGCTCAATCTAAAAAGATCTACATGGCTGTTGTACAAGGTGCAGCGGCAGGGATCG
GCGGGTCGTTAGCCCTAAATTGTGATTTGGTAGTAATGGCTGAGGATGCGTGCTTATTTCAAGCGTTTGCGGCAATAGGC
CTGGTACCTGACGGTGGTGCGAGTTGGCATCTAGTGAATAAGTTAGGCTACAAGAAAGCATTTGAATTATGTGTTGAAGC
CGACAAGCTGACCGCACAAGAGTGCCAAACCGCTGGATTAGTGAATCGCATTTCAGATCAAGCCTCTTTACAAGATGATG
CTTTTGCATGGGCAACAAAATTAGCCAGTGGTGCACCGCTATCCCAACAATATTTAAAGCGTTTATTACAGCAAGCGCAA
AGAGCTAGCTTGCATGACACGATTCGTCAGGAAGCGTTATATCAACAATTCTGCTTTAACAGTGAGGATTTTAAAGAAGG
TGTCAATGCCTTCTTTGACAAACGCGACGCGAGTTTCACTGGCAAGTAA

Upstream 100 bases:

>100_bases
AGACGTCGTGGTGTTAAATATGTCGTTGTTGCAATGTGCATTGGCGGCGGTATGGGCGCCGCAGGGCTATTTGAAGTCAA
TCACTAATTAGGAGACAGTT

Downstream 100 bases:

>100_bases
CTAGTTTATGAATCAAATGGATAATTAGCGCTGTTTTTAAGGACGTTATTGATGAGCGAAGTAATACGGGAAGTAATGGA
GTTTGATGTTGTGATTGTCG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSVILEKAQKLFLSDNDAKVQTGHEFETVNYLVNGKIGIIELNRPDKLNAFNQTMRSELQAAIEMADNNDDLRVIIIRGA
GDHFSSGADLKELVSAKHGIEAQLLNEYKPFLSRIAQSKKIYMAVVQGAAAGIGGSLALNCDLVVMAEDACLFQAFAAIG
LVPDGGASWHLVNKLGYKKAFELCVEADKLTAQECQTAGLVNRISDQASLQDDAFAWATKLASGAPLSQQYLKRLLQQAQ
RASLHDTIRQEALYQQFCFNSEDFKEGVNAFFDKRDASFTGK

Sequences:

>Translated_282_residues
MSVILEKAQKLFLSDNDAKVQTGHEFETVNYLVNGKIGIIELNRPDKLNAFNQTMRSELQAAIEMADNNDDLRVIIIRGA
GDHFSSGADLKELVSAKHGIEAQLLNEYKPFLSRIAQSKKIYMAVVQGAAAGIGGSLALNCDLVVMAEDACLFQAFAAIG
LVPDGGASWHLVNKLGYKKAFELCVEADKLTAQECQTAGLVNRISDQASLQDDAFAWATKLASGAPLSQQYLKRLLQQAQ
RASLHDTIRQEALYQQFCFNSEDFKEGVNAFFDKRDASFTGK
>Mature_281_residues
SVILEKAQKLFLSDNDAKVQTGHEFETVNYLVNGKIGIIELNRPDKLNAFNQTMRSELQAAIEMADNNDDLRVIIIRGAG
DHFSSGADLKELVSAKHGIEAQLLNEYKPFLSRIAQSKKIYMAVVQGAAAGIGGSLALNCDLVVMAEDACLFQAFAAIGL
VPDGGASWHLVNKLGYKKAFELCVEADKLTAQECQTAGLVNRISDQASLQDDAFAWATKLASGAPLSQQYLKRLLQQAQR
ASLHDTIRQEALYQQFCFNSEDFKEGVNAFFDKRDASFTGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=30.6451612903226, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI45643119, Length=254, Percent_Identity=27.1653543307087, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI260275230, Length=254, Percent_Identity=27.1653543307087, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI260274832, Length=254, Percent_Identity=27.1653543307087, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI157694516, Length=221, Percent_Identity=27.1493212669683, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI213417737, Length=221, Percent_Identity=27.1493212669683, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=26.6932270916335, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=26.4, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI25453481, Length=208, Percent_Identity=27.4038461538462, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI51538573, Length=208, Percent_Identity=27.4038461538462, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4757966, Length=208, Percent_Identity=27.4038461538462, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI51538581, Length=208, Percent_Identity=27.4038461538462, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI116235487, Length=228, Percent_Identity=25.4385964912281, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI4757968, Length=175, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI49355787, Length=175, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI157694520, Length=199, Percent_Identity=26.1306532663317, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221136756, Length=193, Percent_Identity=25.9067357512953, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=193, Percent_Identity=25.9067357512953, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=193, Percent_Identity=25.9067357512953, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594469, Length=190, Percent_Identity=29.4736842105263, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594471, Length=190, Percent_Identity=29.4736842105263, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=27.0588235294118, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=32.258064516129, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=31.8897637795276, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=31.6981132075472, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788597, Length=267, Percent_Identity=28.8389513108614, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=26.7676767676768, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=31.5573770491803, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=30.3149606299213, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17535521, Length=249, Percent_Identity=26.1044176706827, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=243, Percent_Identity=27.9835390946502, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=251, Percent_Identity=25.0996015936255, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=193, Percent_Identity=29.0155440414508, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=198, Percent_Identity=26.2626262626263, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=256, Percent_Identity=24.609375, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=25.7383966244726, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323314, Length=203, Percent_Identity=28.0788177339901, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI20129971, Length=264, Percent_Identity=29.5454545454545, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24653477, Length=264, Percent_Identity=29.5454545454545, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24654903, Length=180, Percent_Identity=30.5555555555556, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=27.8884462151394, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920382, Length=257, Percent_Identity=27.6264591439689, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357171, Length=267, Percent_Identity=27.7153558052434, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922422, Length=248, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24650670, Length=189, Percent_Identity=28.042328042328, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30928; Mature: 30797

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVILEKAQKLFLSDNDAKVQTGHEFETVNYLVNGKIGIIELNRPDKLNAFNQTMRSELQ
CCHHHHHHHHHHCCCCCCCEECCCCEEEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHH
AAIEMADNNDDLRVIIIRGAGDHFSSGADLKELVSAKHGIEAQLLNEYKPFLSRIAQSKK
HHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCH
IYMAVVQGAAAGIGGSLALNCDLVVMAEDACLFQAFAAIGLVPDGGASWHLVNKLGYKKA
HHHHHHHHHHCCCCCCEEEECEEEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FELCVEADKLTAQECQTAGLVNRISDQASLQDDAFAWATKLASGAPLSQQYLKRLLQQAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RASLHDTIRQEALYQQFCFNSEDFKEGVNAFFDKRDASFTGK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVILEKAQKLFLSDNDAKVQTGHEFETVNYLVNGKIGIIELNRPDKLNAFNQTMRSELQ
CHHHHHHHHHHCCCCCCCEECCCCEEEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHH
AAIEMADNNDDLRVIIIRGAGDHFSSGADLKELVSAKHGIEAQLLNEYKPFLSRIAQSKK
HHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCH
IYMAVVQGAAAGIGGSLALNCDLVVMAEDACLFQAFAAIGLVPDGGASWHLVNKLGYKKA
HHHHHHHHHHCCCCCCEEEECEEEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FELCVEADKLTAQECQTAGLVNRISDQASLQDDAFAWATKLASGAPLSQQYLKRLLQQAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RASLHDTIRQEALYQQFCFNSEDFKEGVNAFFDKRDASFTGK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA