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The map label for this gene is nylA [H]

Identifier: 167624058

GI number: 167624058

Start: 2569281

End: 2570870

Strand: Direct

Name: nylA [H]

Synonym: Shal_2134

Alternate gene names: 167624058

Gene position: 2569281-2570870 (Clockwise)

Preceding gene: 167624057

Following gene: 167624060

Centisome position: 49.15

GC content: 47.11

Gene sequence:

>1590_bases
ATGAACGATAATATTGATAATAAAAGACGTAGTTTGCTTAAAACAGGTACAGCACTTGGTGCTGCAGCATTGAGTGGAGT
TTCATTAATCGCTCAAGGGGCTGACGCCAGTAACGGACAAAAGGCTAAGGTTGCGAGTGCCGATTTACCATTACCCGAGT
ACGATAACCTTGATGTTGTCGCGATGGCTGAATCTCTTAAAAAAGGTGAACTTACTCCGATAGAGTTGTTTGATGCGGCT
TATGCACGCTTTCAAAGCCGCTCGGCAATCAATGTTGTTGCAGTGGATCATTTTCAACAAGCACGTGAGCATGCTTTGAC
GCTAAGTAAAGCGGGCAGTAAAGAGCGCGCCGACAGTAACGCACCACTTCGTGGTGTTCCTTTCGCGTTAAAAGATCTGC
ATATAAAAGTAAAAGGGACTGTTACTACCAATGGTAGCCCGCTTTTTAAAGACTCGGTAGCAACCAACAACTCCACTCTT
ACTCAGCGGTATATGGACGCAGGCTTGAATATCATGGCTAAGTTGACCAGCCCTGAGTTTGGCCAAACGGCGACCACCGA
ATCGGCTCTTCACGGTGATACGCTAAATCCTTGGGACCTTGCTCGAACTAGCGGCGGCTCAAGCGGAGGCTCTGCGGCAA
CAGTTGCGGCAAGAATATTGCCAGCGGCACATGCAAGTGATGGCGGTGGATCAATCCGAATTCCGGCATCAAACTGTGGT
GTATTTGGTTTAAAGCCGAGTAGGGGACGAGTACCAATGGGCCCAGGAGCTATAGAGGGCTGGATGGGATTGTCAGTTCA
TAATACGGTGACCCGTTCAGTGCGAGATTCGGCACTTTTACTCGACTTAACTCAAGGTAAAGAGGCGGGCTCTCGCATTA
CTCACCAAGATCAGAGCTTAATGGCTGCAATTAAAAAAGCGCCAAAAGGCTTGAAAATAGCGGTGATGAATAACCATCCC
TTCGGTTTGCCCATTCATGCTGACTGCATTAAGGCGGTAAATAAAACTGCCAAGCTGTTAGCTGCACTAGGGCATCATGT
TGAAGAAGCTCAGCCCATATTACCTATCGAAGGTATGTTTAAAGGCATGGGAGTCGCTACAACGAGTGGCTTACTTTACA
CCATTCAAGAGCAAGAAAAATTGCTTGGCCGTAAAGCACGAGAAGAGGAGTTTTCACCCATCGTGTGGCGTCACCTGCAA
AATGCACCTAATTACACTGCTCAGAATGTGTTTAGTGCCCGTAGTGCGTTTGATGACGGTGCAAAAGCGTTTGACCATTT
CTTTAATGATTACCATTTGATTTTGTCACCGGTAACGGCTGTACCACCACCCAAAATCGGTGCGCTAAGCTTAAATCAAC
CTTATGATGATTTTGTGCAAGATATTATGTATGCCTCACCGATTACCGCACTGTTCAATATGACGGGCTTACCCGCTATG
TCGGTTCCTTTACACTGGAACGATGATGGCTTACCTATTGGAGTGCAGTTTGCCGGGCCTTATGGCGGCGAGCCCTTGTT
GTTATCTTTAGCAGCGCAGTTAGAGCAAGCATCGCCGTGGGCAGATAAATTGCCAGGGTTTATTAGTTAA

Upstream 100 bases:

>100_bases
GCACAGAAAGTGTGGGGTAGCTAAGGATTTTAGCTAAAAGCCTTTAGCTGAATTGAGACAATTGACCACGATAATGGATT
AGCACCGAGGACAAATTGCA

Downstream 100 bases:

>100_bases
GCTTCGAATGAAGCTTGATTACATTTTTTAGCTACGGCTACGGGGAAGTGAGTTCTGTGTTGATGCTGCTGGGGGAACAA
TCGCCTGCATCCGATACAAG

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MNDNIDNKRRSLLKTGTALGAAALSGVSLIAQGADASNGQKAKVASADLPLPEYDNLDVVAMAESLKKGELTPIELFDAA
YARFQSRSAINVVAVDHFQQAREHALTLSKAGSKERADSNAPLRGVPFALKDLHIKVKGTVTTNGSPLFKDSVATNNSTL
TQRYMDAGLNIMAKLTSPEFGQTATTESALHGDTLNPWDLARTSGGSSGGSAATVAARILPAAHASDGGGSIRIPASNCG
VFGLKPSRGRVPMGPGAIEGWMGLSVHNTVTRSVRDSALLLDLTQGKEAGSRITHQDQSLMAAIKKAPKGLKIAVMNNHP
FGLPIHADCIKAVNKTAKLLAALGHHVEEAQPILPIEGMFKGMGVATTSGLLYTIQEQEKLLGRKAREEEFSPIVWRHLQ
NAPNYTAQNVFSARSAFDDGAKAFDHFFNDYHLILSPVTAVPPPKIGALSLNQPYDDFVQDIMYASPITALFNMTGLPAM
SVPLHWNDDGLPIGVQFAGPYGGEPLLLSLAAQLEQASPWADKLPGFIS

Sequences:

>Translated_529_residues
MNDNIDNKRRSLLKTGTALGAAALSGVSLIAQGADASNGQKAKVASADLPLPEYDNLDVVAMAESLKKGELTPIELFDAA
YARFQSRSAINVVAVDHFQQAREHALTLSKAGSKERADSNAPLRGVPFALKDLHIKVKGTVTTNGSPLFKDSVATNNSTL
TQRYMDAGLNIMAKLTSPEFGQTATTESALHGDTLNPWDLARTSGGSSGGSAATVAARILPAAHASDGGGSIRIPASNCG
VFGLKPSRGRVPMGPGAIEGWMGLSVHNTVTRSVRDSALLLDLTQGKEAGSRITHQDQSLMAAIKKAPKGLKIAVMNNHP
FGLPIHADCIKAVNKTAKLLAALGHHVEEAQPILPIEGMFKGMGVATTSGLLYTIQEQEKLLGRKAREEEFSPIVWRHLQ
NAPNYTAQNVFSARSAFDDGAKAFDHFFNDYHLILSPVTAVPPPKIGALSLNQPYDDFVQDIMYASPITALFNMTGLPAM
SVPLHWNDDGLPIGVQFAGPYGGEPLLLSLAAQLEQASPWADKLPGFIS
>Mature_529_residues
MNDNIDNKRRSLLKTGTALGAAALSGVSLIAQGADASNGQKAKVASADLPLPEYDNLDVVAMAESLKKGELTPIELFDAA
YARFQSRSAINVVAVDHFQQAREHALTLSKAGSKERADSNAPLRGVPFALKDLHIKVKGTVTTNGSPLFKDSVATNNSTL
TQRYMDAGLNIMAKLTSPEFGQTATTESALHGDTLNPWDLARTSGGSSGGSAATVAARILPAAHASDGGGSIRIPASNCG
VFGLKPSRGRVPMGPGAIEGWMGLSVHNTVTRSVRDSALLLDLTQGKEAGSRITHQDQSLMAAIKKAPKGLKIAVMNNHP
FGLPIHADCIKAVNKTAKLLAALGHHVEEAQPILPIEGMFKGMGVATTSGLLYTIQEQEKLLGRKAREEEFSPIVWRHLQ
NAPNYTAQNVFSARSAFDDGAKAFDHFFNDYHLILSPVTAVPPPKIGALSLNQPYDDFVQDIMYASPITALFNMTGLPAM
SVPLHWNDDGLPIGVQFAGPYGGEPLLLSLAAQLEQASPWADKLPGFIS

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=530, Percent_Identity=26.2264150943396, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI195972892, Length=505, Percent_Identity=23.5643564356436, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=262, Percent_Identity=29.3893129770992, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=554, Percent_Identity=24.3682310469314, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=236, Percent_Identity=29.6610169491525, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=523, Percent_Identity=25.2390057361377, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=306, Percent_Identity=28.1045751633987, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=306, Percent_Identity=28.1045751633987, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=409, Percent_Identity=25.1833740831296, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320448, Length=197, Percent_Identity=34.010152284264, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI161078093, Length=491, Percent_Identity=27.2912423625255, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24644968, Length=265, Percent_Identity=32.4528301886792, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21356731, Length=199, Percent_Identity=34.6733668341709, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI45550774, Length=524, Percent_Identity=24.618320610687, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648435, Length=524, Percent_Identity=24.618320610687, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648437, Length=524, Percent_Identity=24.618320610687, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648113, Length=431, Percent_Identity=29.2343387470998, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648441, Length=446, Percent_Identity=24.8878923766816, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648439, Length=446, Percent_Identity=24.8878923766816, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24652985, Length=194, Percent_Identity=31.9587628865979, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19922090, Length=194, Percent_Identity=31.9587628865979, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24652981, Length=194, Percent_Identity=31.9587628865979, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24652983, Length=194, Percent_Identity=31.9587628865979, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 56136; Mature: 56136

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNIDNKRRSLLKTGTALGAAALSGVSLIAQGADASNGQKAKVASADLPLPEYDNLDVV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCCCHH
AMAESLKKGELTPIELFDAAYARFQSRSAINVVAVDHFQQAREHALTLSKAGSKERADSN
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
APLRGVPFALKDLHIKVKGTVTTNGSPLFKDSVATNNSTLTQRYMDAGLNIMAKLTSPEF
CCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCC
GQTATTESALHGDTLNPWDLARTSGGSSGGSAATVAARILPAAHASDGGGSIRIPASNCG
CCCCCCCHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCC
VFGLKPSRGRVPMGPGAIEGWMGLSVHNTVTRSVRDSALLLDLTQGKEAGSRITHQDQSL
EEECCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHH
MAAIKKAPKGLKIAVMNNHPFGLPIHADCIKAVNKTAKLLAALGHHVEEAQPILPIEGMF
HHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
KGMGVATTSGLLYTIQEQEKLLGRKAREEEFSPIVWRHLQNAPNYTAQNVFSARSAFDDG
CCCCEEECCCEEEEEEHHHHHHCCHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCHH
AKAFDHFFNDYHLILSPVTAVPPPKIGALSLNQPYDDFVQDIMYASPITALFNMTGLPAM
HHHHHHHHCCEEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
SVPLHWNDDGLPIGVQFAGPYGGEPLLLSLAAQLEQASPWADKLPGFIS
EEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCC
>Mature Secondary Structure
MNDNIDNKRRSLLKTGTALGAAALSGVSLIAQGADASNGQKAKVASADLPLPEYDNLDVV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCCCHH
AMAESLKKGELTPIELFDAAYARFQSRSAINVVAVDHFQQAREHALTLSKAGSKERADSN
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
APLRGVPFALKDLHIKVKGTVTTNGSPLFKDSVATNNSTLTQRYMDAGLNIMAKLTSPEF
CCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCC
GQTATTESALHGDTLNPWDLARTSGGSSGGSAATVAARILPAAHASDGGGSIRIPASNCG
CCCCCCCHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCC
VFGLKPSRGRVPMGPGAIEGWMGLSVHNTVTRSVRDSALLLDLTQGKEAGSRITHQDQSL
EEECCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHH
MAAIKKAPKGLKIAVMNNHPFGLPIHADCIKAVNKTAKLLAALGHHVEEAQPILPIEGMF
HHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
KGMGVATTSGLLYTIQEQEKLLGRKAREEEFSPIVWRHLQNAPNYTAQNVFSARSAFDDG
CCCCEEECCCEEEEEEHHHHHHCCHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCHH
AKAFDHFFNDYHLILSPVTAVPPPKIGALSLNQPYDDFVQDIMYASPITALFNMTGLPAM
HHHHHHHHCCEEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
SVPLHWNDDGLPIGVQFAGPYGGEPLLLSLAAQLEQASPWADKLPGFIS
EEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]