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The map label for this gene is kshB [H]

Identifier: 167624026

GI number: 167624026

Start: 2534020

End: 2535105

Strand: Direct

Name: kshB [H]

Synonym: Shal_2102

Alternate gene names: 167624026

Gene position: 2534020-2535105 (Clockwise)

Preceding gene: 167624025

Following gene: 167624027

Centisome position: 48.48

GC content: 40.42

Gene sequence:

>1086_bases
ATGTCTTCATCTCGTTATCACCATTTGACTGTGAGTCATATCATCGAAGAAACTGAAGATTCACGTTCTTTGGTGTTTGA
TTTACCTGAGAGTTTACGTCAAAAGTTTGCATATAAGGCAGGGCAATTCCTTACTTTTAGGATCCCTAAAAACGGCGAGT
ATTTATTACGCTGCTATTCATTATCGAATACGCCAAAAGACTCTTCATTAAAAGTGACCATTAAGCGTGTTCCAAATGGC
TTAGTATCAAGCTGGGTAATGGATGAGGTAAAGGTCGGAGATAAGATAGAAGTAATGCAACCGGCTGGGATTTTTGTCCC
TAAATCCGTTAAGGGAGATATGCTGCTTTGTGCTGGCGGCAGTGGCATTACTCCGGTATTTTCAATATTGCAAACAGCAT
TAGCGCAAGGCGAAGGCAATATTCGTTTGATTTATGCTAATCGTGATGAAAAATCAGTTATTTTCAAGGAACAACTGAAG
GCATTAACCTGTGAATATCCAAATCGCCTTGAAGTTATACACTTAATCGATTCAGTATCAGGGATCCCAAATCCGTACTT
ACTTGCGCGATTAGCCCAGCCATTCTCTGGTTTATTAAACTTTGCTGGTGCGTTTATATGTGGTCCTGGGCCTTTCATGG
ATTCGATGGAAAAAGCACTCGAGTCAATTGATATGTCAGCAGATAAAATTTACATAGAACGCTTTATTTCGTTACCCAAT
GAAAAAATTGTTAAAACAGACGAATTGATAGACGAGCAAAGTATTGCAGCCACTGAAGATGAGCATTTATCACCTGAGTT
AGACCAGCGAATAGATGCCCGAGCGATTATTGAGCTAGATGGACAATCACATTCTGTCAACTGGAGCAAAACCGATACTT
TACTTGAGGCGGCAGAAAAAGCAGGTTTGTCACTTCCACATTCATGTAGGGAAGGGATGTGCGCCTCTTGCATGTGTGAA
GTAAAAGAAGGAAACGTAAAATTACGTATTAATGATGTACTGAGCGAGCGTGATTTAAAGCAATCTTTAACCCTGAGTTG
TCAGGCGGTCCCATTGTCGGAACACATCAGAATTTGTTACACCTAA

Upstream 100 bases:

>100_bases
TACATGCGTGCACATGGTTCGGTCATGCTTGGACTACCAAACCTTGACCCATTTATTTAGTCGCCTTATTAGATAACCCA
TTTGCTGGAAAGGAGTTGCC

Downstream 100 bases:

>100_bases
CACAAATTAACGGGCGTTGTTGTAAAATCGCCCGTTAATTTGCGCTAATGCACTTTTATCAAGTTAAACAAAAATCGCTT
AGTAAAGGCGGTTAATACTC

Product: oxidoreductase FAD-binding subunit

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSSSRYHHLTVSHIIEETEDSRSLVFDLPESLRQKFAYKAGQFLTFRIPKNGEYLLRCYSLSNTPKDSSLKVTIKRVPNG
LVSSWVMDEVKVGDKIEVMQPAGIFVPKSVKGDMLLCAGGSGITPVFSILQTALAQGEGNIRLIYANRDEKSVIFKEQLK
ALTCEYPNRLEVIHLIDSVSGIPNPYLLARLAQPFSGLLNFAGAFICGPGPFMDSMEKALESIDMSADKIYIERFISLPN
EKIVKTDELIDEQSIAATEDEHLSPELDQRIDARAIIELDGQSHSVNWSKTDTLLEAAEKAGLSLPHSCREGMCASCMCE
VKEGNVKLRINDVLSERDLKQSLTLSCQAVPLSEHIRICYT

Sequences:

>Translated_361_residues
MSSSRYHHLTVSHIIEETEDSRSLVFDLPESLRQKFAYKAGQFLTFRIPKNGEYLLRCYSLSNTPKDSSLKVTIKRVPNG
LVSSWVMDEVKVGDKIEVMQPAGIFVPKSVKGDMLLCAGGSGITPVFSILQTALAQGEGNIRLIYANRDEKSVIFKEQLK
ALTCEYPNRLEVIHLIDSVSGIPNPYLLARLAQPFSGLLNFAGAFICGPGPFMDSMEKALESIDMSADKIYIERFISLPN
EKIVKTDELIDEQSIAATEDEHLSPELDQRIDARAIIELDGQSHSVNWSKTDTLLEAAEKAGLSLPHSCREGMCASCMCE
VKEGNVKLRINDVLSERDLKQSLTLSCQAVPLSEHIRICYT
>Mature_360_residues
SSSRYHHLTVSHIIEETEDSRSLVFDLPESLRQKFAYKAGQFLTFRIPKNGEYLLRCYSLSNTPKDSSLKVTIKRVPNGL
VSSWVMDEVKVGDKIEVMQPAGIFVPKSVKGDMLLCAGGSGITPVFSILQTALAQGEGNIRLIYANRDEKSVIFKEQLKA
LTCEYPNRLEVIHLIDSVSGIPNPYLLARLAQPFSGLLNFAGAFICGPGPFMDSMEKALESIDMSADKIYIERFISLPNE
KIVKTDELIDEQSIAATEDEHLSPELDQRIDARAIIELDGQSHSVNWSKTDTLLEAAEKAGLSLPHSCREGMCASCMCEV
KEGNVKLRINDVLSERDLKQSLTLSCQAVPLSEHIRICYT

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=363, Percent_Identity=30.8539944903581, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1787098, Length=350, Percent_Identity=24.5714285714286, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788104, Length=317, Percent_Identity=24.6056782334385, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323552, Length=148, Percent_Identity=33.7837837837838, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322146, Length=167, Percent_Identity=25.1497005988024, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321673, Length=211, Percent_Identity=27.4881516587678, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322699, Length=223, Percent_Identity=23.7668161434978, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24655635, Length=254, Percent_Identity=26.7716535433071, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40117; Mature: 39985

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSRYHHLTVSHIIEETEDSRSLVFDLPESLRQKFAYKAGQFLTFRIPKNGEYLLRCYS
CCCCCEEEEHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCEEEEEECCCCCEEEEEEE
LSNTPKDSSLKVTIKRVPNGLVSSWVMDEVKVGDKIEVMQPAGIFVPKSVKGDMLLCAGG
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCEEEEECC
SGITPVFSILQTALAQGEGNIRLIYANRDEKSVIFKEQLKALTCEYPNRLEVIHLIDSVS
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
GIPNPYLLARLAQPFSGLLNFAGAFICGPGPFMDSMEKALESIDMSADKIYIERFISLPN
CCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EKIVKTDELIDEQSIAATEDEHLSPELDQRIDARAIIELDGQSHSVNWSKTDTLLEAAEK
CCEECHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
AGLSLPHSCREGMCASCMCEVKEGNVKLRINDVLSERDLKQSLTLSCQAVPLSEHIRICY
CCCCCCHHHHCCCHHHHEEEEECCCEEEEEHHHHHHHHHHHHHCEEEEECCHHHCEEEEE
T
C
>Mature Secondary Structure 
SSSRYHHLTVSHIIEETEDSRSLVFDLPESLRQKFAYKAGQFLTFRIPKNGEYLLRCYS
CCCCEEEEHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCEEEEEECCCCCEEEEEEE
LSNTPKDSSLKVTIKRVPNGLVSSWVMDEVKVGDKIEVMQPAGIFVPKSVKGDMLLCAGG
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCEEEEECC
SGITPVFSILQTALAQGEGNIRLIYANRDEKSVIFKEQLKALTCEYPNRLEVIHLIDSVS
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
GIPNPYLLARLAQPFSGLLNFAGAFICGPGPFMDSMEKALESIDMSADKIYIERFISLPN
CCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EKIVKTDELIDEQSIAATEDEHLSPELDQRIDARAIIELDGQSHSVNWSKTDTLLEAAEK
CCEECHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
AGLSLPHSCREGMCASCMCEVKEGNVKLRINDVLSERDLKQSLTLSCQAVPLSEHIRICY
CCCCCCHHHHCCCHHHHEEEEECCCEEEEEHHHHHHHHHHHHHCEEEEECCHHHCEEEEE
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA