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The map label for this gene is gph [H]

Identifier: 167623963

GI number: 167623963

Start: 2457096

End: 2457773

Strand: Reverse

Name: gph [H]

Synonym: Shal_2037

Alternate gene names: 167623963

Gene position: 2457773-2457096 (Counterclockwise)

Preceding gene: 167623964

Following gene: 167623962

Centisome position: 47.02

GC content: 45.58

Gene sequence:

>678_bases
ATGACTAACGCCCACTTTACCCTAATCAAAGGCGTGCTATTTGATTTAGATGGCACGCTGGCCGATACAGCACCAGACAT
GGTAGAAGCGCTTAATATGACGCTACAAGCTCATGGCCACGAGAGAGTGCCGTTAGCTAAGCTCAGAGCCAGTGCCTCAC
ACGGCAGCATTGCAATGATCAGAACGGCCCTGCCCGCAATAGATGAAGAGAGTGTACCGGTATTACAGCAGTCTCTTTTT
GATCACTATCAGCAAATAAACGGTGACAACTGCGACTTGTTTGATGGCTTAGATAGATTACTCGATACGCTCACTCTACT
ATCAATTCCATATGGTGTTGTCACCAATAAGCCAGCTCGTTTCACAAGGCCTTTATTAAATAAGCTTGGCTTAACAGCAA
AAATGCCCGCTGTAGTCAGTGGTGATTCAACTCTTTATTCAAAGCCCCATACCGCTCCTATGCTATTAGCCGCGCAGCAG
CTGAAGGTTCTACCAGAAAACATCCTATATTTAGGAGATGCTGAACGTGATCTTGTGGCGGCAAAGTCCGCTAATATGAT
TTCAGGTTTAGCCGCGTGGGGTTATATCGGACCCGATGATAATCCAAGTAACTGGCCAGCCGACTTCAGTTTTGAAAGTG
GTCACAGCTTAGCCGACTTTTTCAATAAAAAAATTTAG

Upstream 100 bases:

>100_bases
TTCTGTTCTGATGCAGTCGGTATTACCTACAACCCTATCACCGATATCTTTAGATACACCAAGAGTCTTGAAGTTAACTA
TATGATAGCTACGGTTAAAA

Downstream 100 bases:

>100_bases
TGCACCATTTACTATAAAGCTAGCGTGTGTGCAGTTTTGAATCAGTGGTTAAACAAGCTACTCGTTCTCATACCAAAGTA
CTCAAAATGATCTGGACGGA

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTNAHFTLIKGVLFDLDGTLADTAPDMVEALNMTLQAHGHERVPLAKLRASASHGSIAMIRTALPAIDEESVPVLQQSLF
DHYQQINGDNCDLFDGLDRLLDTLTLLSIPYGVVTNKPARFTRPLLNKLGLTAKMPAVVSGDSTLYSKPHTAPMLLAAQQ
LKVLPENILYLGDAERDLVAAKSANMISGLAAWGYIGPDDNPSNWPADFSFESGHSLADFFNKKI

Sequences:

>Translated_225_residues
MTNAHFTLIKGVLFDLDGTLADTAPDMVEALNMTLQAHGHERVPLAKLRASASHGSIAMIRTALPAIDEESVPVLQQSLF
DHYQQINGDNCDLFDGLDRLLDTLTLLSIPYGVVTNKPARFTRPLLNKLGLTAKMPAVVSGDSTLYSKPHTAPMLLAAQQ
LKVLPENILYLGDAERDLVAAKSANMISGLAAWGYIGPDDNPSNWPADFSFESGHSLADFFNKKI
>Mature_224_residues
TNAHFTLIKGVLFDLDGTLADTAPDMVEALNMTLQAHGHERVPLAKLRASASHGSIAMIRTALPAIDEESVPVLQQSLFD
HYQQINGDNCDLFDGLDRLLDTLTLLSIPYGVVTNKPARFTRPLLNKLGLTAKMPAVVSGDSTLYSKPHTAPMLLAAQQL
KVLPENILYLGDAERDLVAAKSANMISGLAAWGYIGPDDNPSNWPADFSFESGHSLADFFNKKI

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=212, Percent_Identity=33.0188679245283, Blast_Score=104, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24414; Mature: 24283

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAHFTLIKGVLFDLDGTLADTAPDMVEALNMTLQAHGHERVPLAKLRASASHGSIAMI
CCCCHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHH
RTALPAIDEESVPVLQQSLFDHYQQINGDNCDLFDGLDRLLDTLTLLSIPYGVVTNKPAR
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHH
FTRPLLNKLGLTAKMPAVVSGDSTLYSKPHTAPMLLAAQQLKVLPENILYLGDAERDLVA
HHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCHHHHH
AKSANMISGLAAWGYIGPDDNPSNWPADFSFESGHSLADFFNKKI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TNAHFTLIKGVLFDLDGTLADTAPDMVEALNMTLQAHGHERVPLAKLRASASHGSIAMI
CCCHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHH
RTALPAIDEESVPVLQQSLFDHYQQINGDNCDLFDGLDRLLDTLTLLSIPYGVVTNKPAR
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHH
FTRPLLNKLGLTAKMPAVVSGDSTLYSKPHTAPMLLAAQQLKVLPENILYLGDAERDLVA
HHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCHHHHH
AKSANMISGLAAWGYIGPDDNPSNWPADFSFESGHSLADFFNKKI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]