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The map label for this gene is nrdA [H]

Identifier: 167623962

GI number: 167623962

Start: 2454338

End: 2456626

Strand: Reverse

Name: nrdA [H]

Synonym: Shal_2036

Alternate gene names: 167623962

Gene position: 2456626-2454338 (Counterclockwise)

Preceding gene: 167623963

Following gene: 167623961

Centisome position: 47.0

GC content: 43.73

Gene sequence:

>2289_bases
ATGAATAGCAATATGACAGTCACAAAGCGTAGTGGCGAGCGTGAGACTATCGATCTCGACAAAATCCATCGTGTTATTGA
ATGGGCGGCAAAGGGACTTAACAATGTCTCAGTCTCAGAAGTAGAACTGCGTTCTCACCTGCAATTTTTCGACGGCATTG
CAACTGAAGCCATCCATGAGACTATCATTAAAGCGGCTGCTGACTTAATTTCCCCCGAAGCACCTGATTATCAGTTTCTT
TCTGCTCGTCTTGCTGTATTCCATTTACGTAAGAAAGCCTTCGGCCAATTTGAACCACCGAAACTATACGATCATGTCGT
TAAACTTGTTGAGCAAGGCAAGTATGACGCACACATTCTAGAAGATTACAGCCAAGAAGAGTTAGATATTCTTGATGGCT
ATATCGATCATTGGCGCGATATGAACTTCTCTTATGCTGCGGTTAAGCAGTTAGAAGGTAAATATCTAGTTCAAAATCGT
GTTAGCCATGAGATCTACGAAAGTGCGCAATTCCTGTATATTTTGGTTGCAGCCTGTCTTTTTGCTAAGTATCCAAAAGA
GACGCGCCTGAAATACGTAAAAGACTTCTATGATGCGACATCATTATTCAAAATTTCACTGCCTACGCCAATTATGGCTG
GCGTTCGCACGCCAACTCGTCAGTTCAGCTCATGTGTACTGATTGAATGTGGTGATAGCCTAGACTCAATTAACGCCACA
TCATCATCAATCGTTAAGTATGTTTCTCAACGTGCCGGTATTGGTGTCAATGCAGGCCGTATTCGCGCACTTGGTAGCCC
TATCCGCGGTGGCGAAGCATTCCATACAGGTTGCTTACCATTTTATAAATACTTCCAAACAGCGGTAAAGTCGTGCTCTC
AAGGCGGCGTACGCGGTGGCGCTGCTACCTTGTTCTACCCAATGTGGCATCTTGAAGTTGAGTCGCTGTTGGTACTTAAG
AACAACCGTGGTGTTGATGATAACCGTGTTCGTCATTTGGATTATGGCGTTCAAATCAACAAGGTGATGTATCAACGCCT
TATCCAAGGCGGCATGATCAGCTTATTTAGTCCATCAGATGTGCCTGGAATGTACGATGCATTCTTTGAAGATCAACAAG
AGTTTGAGCGCTTATATCTTAAATATGAAGCCGATGACAGCATTCGTAAGAAGCAGATTAAAGCGGTAGAGTTATTCTCG
CTGATGATGCAAGAGCGTGCCTCTACGGGCCGCATTTATATTCAGAACGTGGATCACTGTAACACTCACAGTCCATTTGA
CTCTAAAGTGGCACCAGTTCGTCAATCTAACTTGTGCCTTGAAATTGCACTACCCACTAAGCCGTTAAACAACATTAACG
ACCCTGATGGTGAGATTGCACTTTGTACACTTTCAGCATTGAACTTAGGTGCAATTAAGAAGTTAGATGAACTTGAGCCA
CTGGCTGATCTTGCCGTACGTGCACTTGATAACCTGCTTGATTACCAAGATTACCCAATCATCTCTGCTAAGAAAGCATC
GATGAATCGCCGTACTCTAGGTATAGGCGTAATCAACTTTGCCAACTATTTAGCGAAAGAAGGCGTACGTTACTCAGACG
GTTCAGCTAACGGCATTACCCATAAAACATTTGAAGCCATTCAATTTTACTTATTGAAAGCCTCAATGAAGCTTGCTCAA
GAGCAAGGGGCTTGTCCAGCGTTTCATGAAACCACTTACGCTAAGGGTATTCTACCCATTGATACCTACAAGCGTGATCT
AGATAAGATTTGCGATGAACCATTGCACCTCGACTGGGAAACACTGCGCGAAGAGATCAAGACACACGGTCTACGTAACT
CTACGCTTTCAGCGCTAATGCCGTCAGAGACCTCTTCACAGATCTCTAACGCAACTAACGGTATTGAGCCACCCCGTGGT
TTAATCAGTGTTAAAGCGAGTAAAGATGGCCAGCTAAAGCAAGTGGTACCTGATTTTGAAAAATATCAGTATAGCTACGA
GCTATTATGGCAAATGCCTAATAACGATGGTTACTTGCAGTTAGTCGGATTGATGCAAAAGTTTGTTGATCAGTCTATTT
CGGCGAATACCAACTACGACCCTACTCGTTTTGAGGGGGGCCGTGTACCGATGAAGGTACTATTGAAAGACCTGTTAACC
GCTTATAAATATGGTGTGAAGACACTTTACTATCACAATACTCGTGATGGGGCTTCAGATAGTCTTGACGATATCACTGC
CATTGAGAAAGAAGATGACAGTTGCGCTGGCGGCGCATGTAAGATCTAA

Upstream 100 bases:

>100_bases
ACCTAGCGTTTTTACGGTTAAAAACGGTTTGTTTACAGGGAAACGTAGGGTGAACCTGAACGGGTCACTTTCATAAATAT
AAAAATCAAGGTCTATTTTA

Downstream 100 bases:

>100_bases
CCCAAGAGTTAATCATTAGTATTAATCGTTAATTTAAGGGGGCCTAGCCCCCATTATCGGAAGATAGAAAAATGGCCTAT
TCTACATTTTGTCAAACACC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 762; Mature: 762

Protein sequence:

>762_residues
MNSNMTVTKRSGERETIDLDKIHRVIEWAAKGLNNVSVSEVELRSHLQFFDGIATEAIHETIIKAAADLISPEAPDYQFL
SARLAVFHLRKKAFGQFEPPKLYDHVVKLVEQGKYDAHILEDYSQEELDILDGYIDHWRDMNFSYAAVKQLEGKYLVQNR
VSHEIYESAQFLYILVAACLFAKYPKETRLKYVKDFYDATSLFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
SSSIVKYVSQRAGIGVNAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLK
NNRGVDDNRVRHLDYGVQINKVMYQRLIQGGMISLFSPSDVPGMYDAFFEDQQEFERLYLKYEADDSIRKKQIKAVELFS
LMMQERASTGRIYIQNVDHCNTHSPFDSKVAPVRQSNLCLEIALPTKPLNNINDPDGEIALCTLSALNLGAIKKLDELEP
LADLAVRALDNLLDYQDYPIISAKKASMNRRTLGIGVINFANYLAKEGVRYSDGSANGITHKTFEAIQFYLLKASMKLAQ
EQGACPAFHETTYAKGILPIDTYKRDLDKICDEPLHLDWETLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRG
LISVKASKDGQLKQVVPDFEKYQYSYELLWQMPNNDGYLQLVGLMQKFVDQSISANTNYDPTRFEGGRVPMKVLLKDLLT
AYKYGVKTLYYHNTRDGASDSLDDITAIEKEDDSCAGGACKI

Sequences:

>Translated_762_residues
MNSNMTVTKRSGERETIDLDKIHRVIEWAAKGLNNVSVSEVELRSHLQFFDGIATEAIHETIIKAAADLISPEAPDYQFL
SARLAVFHLRKKAFGQFEPPKLYDHVVKLVEQGKYDAHILEDYSQEELDILDGYIDHWRDMNFSYAAVKQLEGKYLVQNR
VSHEIYESAQFLYILVAACLFAKYPKETRLKYVKDFYDATSLFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
SSSIVKYVSQRAGIGVNAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLK
NNRGVDDNRVRHLDYGVQINKVMYQRLIQGGMISLFSPSDVPGMYDAFFEDQQEFERLYLKYEADDSIRKKQIKAVELFS
LMMQERASTGRIYIQNVDHCNTHSPFDSKVAPVRQSNLCLEIALPTKPLNNINDPDGEIALCTLSALNLGAIKKLDELEP
LADLAVRALDNLLDYQDYPIISAKKASMNRRTLGIGVINFANYLAKEGVRYSDGSANGITHKTFEAIQFYLLKASMKLAQ
EQGACPAFHETTYAKGILPIDTYKRDLDKICDEPLHLDWETLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRG
LISVKASKDGQLKQVVPDFEKYQYSYELLWQMPNNDGYLQLVGLMQKFVDQSISANTNYDPTRFEGGRVPMKVLLKDLLT
AYKYGVKTLYYHNTRDGASDSLDDITAIEKEDDSCAGGACKI
>Mature_762_residues
MNSNMTVTKRSGERETIDLDKIHRVIEWAAKGLNNVSVSEVELRSHLQFFDGIATEAIHETIIKAAADLISPEAPDYQFL
SARLAVFHLRKKAFGQFEPPKLYDHVVKLVEQGKYDAHILEDYSQEELDILDGYIDHWRDMNFSYAAVKQLEGKYLVQNR
VSHEIYESAQFLYILVAACLFAKYPKETRLKYVKDFYDATSLFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
SSSIVKYVSQRAGIGVNAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLK
NNRGVDDNRVRHLDYGVQINKVMYQRLIQGGMISLFSPSDVPGMYDAFFEDQQEFERLYLKYEADDSIRKKQIKAVELFS
LMMQERASTGRIYIQNVDHCNTHSPFDSKVAPVRQSNLCLEIALPTKPLNNINDPDGEIALCTLSALNLGAIKKLDELEP
LADLAVRALDNLLDYQDYPIISAKKASMNRRTLGIGVINFANYLAKEGVRYSDGSANGITHKTFEAIQFYLLKASMKLAQ
EQGACPAFHETTYAKGILPIDTYKRDLDKICDEPLHLDWETLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRG
LISVKASKDGQLKQVVPDFEKYQYSYELLWQMPNNDGYLQLVGLMQKFVDQSISANTNYDPTRFEGGRVPMKVLLKDLLT
AYKYGVKTLYYHNTRDGASDSLDDITAIEKEDDSCAGGACKI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-cone domain [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=678, Percent_Identity=28.6135693215339, Blast_Score=225, Evalue=1e-58,
Organism=Escherichia coli, GI2367133, Length=762, Percent_Identity=80.9711286089239, Blast_Score=1310, Evalue=0.0,
Organism=Escherichia coli, GI1789030, Length=668, Percent_Identity=24.4011976047904, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17555242, Length=693, Percent_Identity=27.8499278499279, Blast_Score=235, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6322123, Length=674, Percent_Identity=28.7833827893175, Blast_Score=245, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6320914, Length=674, Percent_Identity=27.893175074184, Blast_Score=240, Evalue=5e-64,
Organism=Drosophila melanogaster, GI17352481, Length=676, Percent_Identity=29.585798816568, Blast_Score=245, Evalue=9e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 85679; Mature: 85679

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNMTVTKRSGERETIDLDKIHRVIEWAAKGLNNVSVSEVELRSHLQFFDGIATEAIHE
CCCCCEEEECCCCCCEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TIIKAAADLISPEAPDYQFLSARLAVFHLRKKAFGQFEPPKLYDHVVKLVEQGKYDAHIL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHH
EDYSQEELDILDGYIDHWRDMNFSYAAVKQLEGKYLVQNRVSHEIYESAQFLYILVAACL
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAKYPKETRLKYVKDFYDATSLFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
HHHCCHHHHHHHHHHHHCCHHEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCCCCCCCC
SSSIVKYVSQRAGIGVNAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
AATLFYPMWHLEVESLLVLKNNRGVDDNRVRHLDYGVQINKVMYQRLIQGGMISLFSPSD
CHHEEHHHHHHEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCC
VPGMYDAFFEDQQEFERLYLKYEADDSIRKKQIKAVELFSLMMQERASTGRIYIQNVDHC
CCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDSKVAPVRQSNLCLEIALPTKPLNNINDPDGEIALCTLSALNLGAIKKLDELEP
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEHHHCHHHHHHHHHHHH
LADLAVRALDNLLDYQDYPIISAKKASMNRRTLGIGVINFANYLAKEGVRYSDGSANGIT
HHHHHHHHHHHHHCCCCCCCEECHHHCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCC
HKTFEAIQFYLLKASMKLAQEQGACPAFHETTYAKGILPIDTYKRDLDKICDEPLHLDWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHH
TLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGLISVKASKDGQLKQVVPDFE
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCHH
KYQYSYELLWQMPNNDGYLQLVGLMQKFVDQSISANTNYDPTRFEGGRVPMKVLLKDLLT
HHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKYGVKTLYYHNTRDGASDSLDDITAIEKEDDSCAGGACKI
HHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSNMTVTKRSGERETIDLDKIHRVIEWAAKGLNNVSVSEVELRSHLQFFDGIATEAIHE
CCCCCEEEECCCCCCEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TIIKAAADLISPEAPDYQFLSARLAVFHLRKKAFGQFEPPKLYDHVVKLVEQGKYDAHIL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHH
EDYSQEELDILDGYIDHWRDMNFSYAAVKQLEGKYLVQNRVSHEIYESAQFLYILVAACL
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAKYPKETRLKYVKDFYDATSLFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
HHHCCHHHHHHHHHHHHCCHHEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCCCCCCCC
SSSIVKYVSQRAGIGVNAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
AATLFYPMWHLEVESLLVLKNNRGVDDNRVRHLDYGVQINKVMYQRLIQGGMISLFSPSD
CHHEEHHHHHHEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCC
VPGMYDAFFEDQQEFERLYLKYEADDSIRKKQIKAVELFSLMMQERASTGRIYIQNVDHC
CCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDSKVAPVRQSNLCLEIALPTKPLNNINDPDGEIALCTLSALNLGAIKKLDELEP
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEHHHCHHHHHHHHHHHH
LADLAVRALDNLLDYQDYPIISAKKASMNRRTLGIGVINFANYLAKEGVRYSDGSANGIT
HHHHHHHHHHHHHCCCCCCCEECHHHCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCC
HKTFEAIQFYLLKASMKLAQEQGACPAFHETTYAKGILPIDTYKRDLDKICDEPLHLDWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHH
TLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGLISVKASKDGQLKQVVPDFE
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCHH
KYQYSYELLWQMPNNDGYLQLVGLMQKFVDQSISANTNYDPTRFEGGRVPMKVLLKDLLT
HHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKYGVKTLYYHNTRDGASDSLDDITAIEKEDDSCAGGACKI
HHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6087316; 3287341; 9205837; 9278503; 3894026; 2663852; 8702814; 8052308; 9309223; 10493864 [H]