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The map label for this gene is nrdB [H]

Identifier: 167623961

GI number: 167623961

Start: 2453136

End: 2454266

Strand: Reverse

Name: nrdB [H]

Synonym: Shal_2035

Alternate gene names: 167623961

Gene position: 2454266-2453136 (Counterclockwise)

Preceding gene: 167623962

Following gene: 167623960

Centisome position: 46.95

GC content: 42.0

Gene sequence:

>1131_bases
ATGGCCTATTCTACATTTTGTCAAACACCAAATAATGCACTGCTAGAGCCTATGTTTCTTGGGCAATCAGTAAACGTAGC
GCGTTACGATCAACAAAAATATGAAGTATTTGAAAAGCTAATCGAAAAGCAACTTTCCTTCTTCTGGCGCCCAGAAGAAG
TCGACGTCAGCCGTGATAAAATCGATTACGCAGCACTACCTGAGCATGAGAAGCACATCTTTATCTCAAACCTTAAATAC
CAAACACTACTTGACTCAATCCAAGGCCGCTCACCAAACGTTGCCTTCTTGCCTTTGGTATCACTACCAGAGCTAGAGAC
TTGGATTGAAACTTGGTCTTTCTCAGAGACAATTCACTCGCGCTCATACACCCATATTATTCGTAACATTGTTAACGATC
CTTCAATCGTGTTTGACGATATCGTTGAAAACGAAGAGATCTTAAAGCGTGCAAGCGATATCGCTAAGTACTACGACCAC
CTGATCAAGCTTAGCCAGGCTTACCATTTGTTGGGCGAAGGTAGCCACGAGATCGACGGTGAAATCATTGAAGTCACAAC
ACGTGAAATCAAAAAAGCCCTGTACTTGTGTATGGTATCGGTCAACGTACTTGAAGCTATCCGTTTCTACGTCAGCTTTG
CCTGCTCGTTTGCCTTTGCAGAGCGCAAGGTCATGGAAGGTAATGCCAAGATCATTCGTCTAATTGCTCGTGATGAAGCA
TTGCACCTTAACAGTACGCAACACATTTTAAAGATCATGCAAGGTGGTAAAGATGATCCAGAAATGGCTGAGATTGCAAA
AGAGTGTGAACAAACAGCTATTGATATCTTTGTAAAAGCTGCTGAACAGGAAAAAGAGTGGGCTAAATACCTATTTAAAG
ATGGTTCAATGATTGGTCTAAACGAAACAATCCTGTGCCAATATGTTGAATACATCACCAACGAACGCATGAAATCCGTT
AATTTTGCATCACCATATGCTGAACAGACCAACCCTCTTCCATGGATGAAGAACTGGTTAGAAAGTGATTCAGTGCAAGT
TGCGCCTCAAGAAGTAGAAGTCTCCTCTTACCTTGTGGGTCAAATCGATTCATCAATCGATGACGATGAGTTCTCAGACT
TTGACCTATAA

Upstream 100 bases:

>100_bases
GTTGCGCTGGCGGCGCATGTAAGATCTAACCCAAGAGTTAATCATTAGTATTAATCGTTAATTTAAGGGGGCCTAGCCCC
CATTATCGGAAGATAGAAAA

Downstream 100 bases:

>100_bases
ACCACAAGACTTTAGTCAAGCAAGCTTTAAGAAGGCTCCGATTGTGAGCCTTCAAGGCATGCCTGTGTTGCTGTTTAATC
AGCAACACTTAACCCTGCTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Protein B2; Protein R2; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAYSTFCQTPNNALLEPMFLGQSVNVARYDQQKYEVFEKLIEKQLSFFWRPEEVDVSRDKIDYAALPEHEKHIFISNLKY
QTLLDSIQGRSPNVAFLPLVSLPELETWIETWSFSETIHSRSYTHIIRNIVNDPSIVFDDIVENEEILKRASDIAKYYDH
LIKLSQAYHLLGEGSHEIDGEIIEVTTREIKKALYLCMVSVNVLEAIRFYVSFACSFAFAERKVMEGNAKIIRLIARDEA
LHLNSTQHILKIMQGGKDDPEMAEIAKECEQTAIDIFVKAAEQEKEWAKYLFKDGSMIGLNETILCQYVEYITNERMKSV
NFASPYAEQTNPLPWMKNWLESDSVQVAPQEVEVSSYLVGQIDSSIDDDEFSDFDL

Sequences:

>Translated_376_residues
MAYSTFCQTPNNALLEPMFLGQSVNVARYDQQKYEVFEKLIEKQLSFFWRPEEVDVSRDKIDYAALPEHEKHIFISNLKY
QTLLDSIQGRSPNVAFLPLVSLPELETWIETWSFSETIHSRSYTHIIRNIVNDPSIVFDDIVENEEILKRASDIAKYYDH
LIKLSQAYHLLGEGSHEIDGEIIEVTTREIKKALYLCMVSVNVLEAIRFYVSFACSFAFAERKVMEGNAKIIRLIARDEA
LHLNSTQHILKIMQGGKDDPEMAEIAKECEQTAIDIFVKAAEQEKEWAKYLFKDGSMIGLNETILCQYVEYITNERMKSV
NFASPYAEQTNPLPWMKNWLESDSVQVAPQEVEVSSYLVGQIDSSIDDDEFSDFDL
>Mature_375_residues
AYSTFCQTPNNALLEPMFLGQSVNVARYDQQKYEVFEKLIEKQLSFFWRPEEVDVSRDKIDYAALPEHEKHIFISNLKYQ
TLLDSIQGRSPNVAFLPLVSLPELETWIETWSFSETIHSRSYTHIIRNIVNDPSIVFDDIVENEEILKRASDIAKYYDHL
IKLSQAYHLLGEGSHEIDGEIIEVTTREIKKALYLCMVSVNVLEAIRFYVSFACSFAFAERKVMEGNAKIIRLIARDEAL
HLNSTQHILKIMQGGKDDPEMAEIAKECEQTAIDIFVKAAEQEKEWAKYLFKDGSMIGLNETILCQYVEYITNERMKSVN
FASPYAEQTNPLPWMKNWLESDSVQVAPQEVEVSSYLVGQIDSSIDDDEFSDFDL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1788567, Length=376, Percent_Identity=74.468085106383, Blast_Score=613, Evalue=1e-177,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 43364; Mature: 43232

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSTFCQTPNNALLEPMFLGQSVNVARYDQQKYEVFEKLIEKQLSFFWRPEEVDVSRDK
CCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHC
IDYAALPEHEKHIFISNLKYQTLLDSIQGRSPNVAFLPLVSLPELETWIETWSFSETIHS
CCHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RSYTHIIRNIVNDPSIVFDDIVENEEILKRASDIAKYYDHLIKLSQAYHLLGEGSHEIDG
HHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EIIEVTTREIKKALYLCMVSVNVLEAIRFYVSFACSFAFAERKVMEGNAKIIRLIARDEA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCC
LHLNSTQHILKIMQGGKDDPEMAEIAKECEQTAIDIFVKAAEQEKEWAKYLFKDGSMIGL
EECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
NETILCQYVEYITNERMKSVNFASPYAEQTNPLPWMKNWLESDSVQVAPQEVEVSSYLVG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHH
QIDSSIDDDEFSDFDL
HHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYSTFCQTPNNALLEPMFLGQSVNVARYDQQKYEVFEKLIEKQLSFFWRPEEVDVSRDK
CCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHC
IDYAALPEHEKHIFISNLKYQTLLDSIQGRSPNVAFLPLVSLPELETWIETWSFSETIHS
CCHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RSYTHIIRNIVNDPSIVFDDIVENEEILKRASDIAKYYDHLIKLSQAYHLLGEGSHEIDG
HHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EIIEVTTREIKKALYLCMVSVNVLEAIRFYVSFACSFAFAERKVMEGNAKIIRLIARDEA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCC
LHLNSTQHILKIMQGGKDDPEMAEIAKECEQTAIDIFVKAAEQEKEWAKYLFKDGSMIGL
EECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
NETILCQYVEYITNERMKSVNFASPYAEQTNPLPWMKNWLESDSVQVAPQEVEVSSYLVG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHH
QIDSSIDDDEFSDFDL
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]