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The map label for this gene is yafJ [H]

Identifier: 167623821

GI number: 167623821

Start: 2273965

End: 2274750

Strand: Direct

Name: yafJ [H]

Synonym: Shal_1891

Alternate gene names: 167623821

Gene position: 2273965-2274750 (Clockwise)

Preceding gene: 167623819

Following gene: 167623824

Centisome position: 43.5

GC content: 47.46

Gene sequence:

>786_bases
ATGTGCGAGTTGCTGGCCATGAGTGCCAATGTTCCGACAGATATTGTATTTAGTTTTACTGGATTAGCACAACGAGGTGG
TGTTACAGGGCCCCACGTCGATGGTTGGGGGATCACGTTTTACGAAGGTAAGGGCAACCGTACTTTTAAAGATGCACGCC
CTAGCAGTGAGTCCCATATTGCACAGCTCATTAAGTCCTATCCGATAAAAAGCGAAGTGGTCGTGAGCCATATTCGCCAA
GCTAACCGCGGCTGTGTGTCATTAGAAAATACTCACCCCTTTACTCGAGAGCTTTGGGGGCAATATTGGACCTATGCTCA
CAACGGCCAGTTGAGTGACTATGAGAAAAAGTTTGTTTCGACACGCTTTAATGCCGTAGGGGATACCGATAGCGAGATGG
CTTTTTGCTGGATCATGGACAAAGTGGTGGAGAAGTTTGGCGATAAGGCTCCTGACGATAGGTTGGCTGTATATCGCTAT
ATCTCGACGTTGGCCGATGAAATTCGCGCCTTAGGTGTGTTCAACATGATCTTAAGTGATGGTGAACACCTAATGAGTTA
CTGCAGCAATAACCTCTGCTATATCACCCGCCGCGCGCCGTTTGGTAAAGCAAAGCTCATCGATACTGATGTGGTGATAG
ACTTCGATAAAGAGACCACTCCGAACGATGTGGTGACGGTAATCGCCACACGTCCATTGACCAATAATGAGCAATGGAAC
GTGTTAACCCCAGGCGAATGGAAGTTGTTTAAGAAAGGGGAGCTGGTTAGTTCGTCTGTTAGCTAA

Upstream 100 bases:

>100_bases
TTTAAATTCTGTGTTTTCTAAAAAATATTCCAGAATGGCTATCAAGTGGTTAAAATGGTGTAATTAGAGGTAGGTAGTCG
ATTAAACGGGATAAAGATTA

Downstream 100 bases:

>100_bases
TCTGTCCTGAAATTAAAAAGCTCGCTAAGCCTAAGTTAGCGAGCTTTTTTGTTATCTCTAGCTCAGAAAATAGTTGAGTC
TTGTCGCTCGCCTTCTTAAC

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGKGNRTFKDARPSSESHIAQLIKSYPIKSEVVVSHIRQ
ANRGCVSLENTHPFTRELWGQYWTYAHNGQLSDYEKKFVSTRFNAVGDTDSEMAFCWIMDKVVEKFGDKAPDDRLAVYRY
ISTLADEIRALGVFNMILSDGEHLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTNNEQWN
VLTPGEWKLFKKGELVSSSVS

Sequences:

>Translated_261_residues
MCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGKGNRTFKDARPSSESHIAQLIKSYPIKSEVVVSHIRQ
ANRGCVSLENTHPFTRELWGQYWTYAHNGQLSDYEKKFVSTRFNAVGDTDSEMAFCWIMDKVVEKFGDKAPDDRLAVYRY
ISTLADEIRALGVFNMILSDGEHLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTNNEQWN
VLTPGEWKLFKKGELVSSSVS
>Mature_261_residues
MCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGKGNRTFKDARPSSESHIAQLIKSYPIKSEVVVSHIRQ
ANRGCVSLENTHPFTRELWGQYWTYAHNGQLSDYEKKFVSTRFNAVGDTDSEMAFCWIMDKVVEKFGDKAPDDRLAVYRY
ISTLADEIRALGVFNMILSDGEHLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTNNEQWN
VLTPGEWKLFKKGELVSSSVS

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=256, Percent_Identity=66.796875, Blast_Score=360, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29341; Mature: 29341

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGKGNRTFKDARPSSESHI
CCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
AQLIKSYPIKSEVVVSHIRQANRGCVSLENTHPFTRELWGQYWTYAHNGQLSDYEKKFVS
HHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCEEECCCCCCHHHHHHHH
TRFNAVGDTDSEMAFCWIMDKVVEKFGDKAPDDRLAVYRYISTLADEIRALGVFNMILSD
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEHLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTNNEQWN
HHHHHHHHCCCEEEEEECCCCCCCEEEECEEEEECCCCCCCCCEEEEEEECCCCCCCCEE
VLTPGEWKLFKKGELVSSSVS
EECCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGKGNRTFKDARPSSESHI
CCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
AQLIKSYPIKSEVVVSHIRQANRGCVSLENTHPFTRELWGQYWTYAHNGQLSDYEKKFVS
HHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCEEECCCCCCHHHHHHHH
TRFNAVGDTDSEMAFCWIMDKVVEKFGDKAPDDRLAVYRYISTLADEIRALGVFNMILSD
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEHLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTNNEQWN
HHHHHHHHCCCEEEEEECCCCCCCEEEECEEEEECCCCCCCCCEEEEEEECCCCCCCCEE
VLTPGEWKLFKKGELVSSSVS
EECCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]