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The map label for this gene is rnhA

Identifier: 167623804

GI number: 167623804

Start: 2247813

End: 2248298

Strand: Reverse

Name: rnhA

Synonym: Shal_1874

Alternate gene names: 167623804

Gene position: 2248298-2247813 (Counterclockwise)

Preceding gene: 167623815

Following gene: 167623801

Centisome position: 43.01

GC content: 48.35

Gene sequence:

>486_bases
ATGACTGGACTGAAACAGATCTCTATCTATACCGATGGTTCTTGCCTCGGAAATCCTGGCCCTGGCGGTTATGGCATCGT
TTTAAAGTATAAAAAGCAAACAAAAGAGCTCGCTGATGGTTTTGCCCTCACCACTAACAATCGTATGGAGCTATTGGCCC
CGATTGTCGCTCTGGAAGTGTTGAAAGTCCCCTGCCAAGTGATCTTAACCAGCGACAGTCAATATATGCGCCAAGGGATC
ACCCAGTGGATCCACGGTTGGAAGCGTAAAGGTTGGCTCACCTCTGCGGGTCAACCAGTAAAAAATGTCGACTTATGGAA
ACGTCTGGACACGGTATCACAACGTCATCAAATTGACTGGCGTTGGGTTAAAGGCCACGCAGGGCATACAGAAAATGAGC
GTTGTGACGACCTTGCACGCCAAGCTGCCGAAGCTAAACCCTCACAAGAAGATAGCGGTTATATTAATCAGCAGGCTCAA
GCCTAA

Upstream 100 bases:

>100_bases
TCGCTTCGCGCTTGAAACAATATTCATATGAATTTATGCATTTCTCAGTTAAATTTTTTGGGTATACTCGCCCAGCAATT
ATAGCTATGGTAGCAACTTG

Downstream 100 bases:

>100_bases
TCCCCTCGGGTATAGCACTCTCAAGGGCTAATAGATGACAAGCTCGACGTCACTCATTAGCCTAGTACTTCTTAATAGCT
TAACAAGCTACCAAGGTAAG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MTGLKQISIYTDGSCLGNPGPGGYGIVLKYKKQTKELADGFALTTNNRMELLAPIVALEVLKVPCQVILTSDSQYMRQGI
TQWIHGWKRKGWLTSAGQPVKNVDLWKRLDTVSQRHQIDWRWVKGHAGHTENERCDDLARQAAEAKPSQEDSGYINQQAQ
A

Sequences:

>Translated_161_residues
MTGLKQISIYTDGSCLGNPGPGGYGIVLKYKKQTKELADGFALTTNNRMELLAPIVALEVLKVPCQVILTSDSQYMRQGI
TQWIHGWKRKGWLTSAGQPVKNVDLWKRLDTVSQRHQIDWRWVKGHAGHTENERCDDLARQAAEAKPSQEDSGYINQQAQ
A
>Mature_160_residues
TGLKQISIYTDGSCLGNPGPGGYGIVLKYKKQTKELADGFALTTNNRMELLAPIVALEVLKVPCQVILTSDSQYMRQGIT
QWIHGWKRKGWLTSAGQPVKNVDLWKRLDTVSQRHQIDWRWVKGHAGHTENERCDDLARQAAEAKPSQEDSGYINQQAQA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=143, Percent_Identity=35.6643356643357, Blast_Score=80, Evalue=8e-16,
Organism=Escherichia coli, GI1786408, Length=151, Percent_Identity=66.2251655629139, Blast_Score=221, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=37.5886524822695, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI17137476, Length=162, Percent_Identity=36.4197530864198, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45552509, Length=162, Percent_Identity=36.4197530864198, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_SHEHH (B0TRM1)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674098.1
- ProteinModelPortal:   B0TRM1
- SMR:   B0TRM1
- GeneID:   5904031
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1874
- HOGENOM:   HBG742437
- OMA:   SKVVIYT
- ProtClustDB:   PRK00203
- BioCyc:   SHAL458817:SHAL_1874-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 18050; Mature: 17919

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLKQISIYTDGSCLGNPGPGGYGIVLKYKKQTKELADGFALTTNNRMELLAPIVALEV
CCCCEEEEEEECCCCCCCCCCCCEEEEEEECHHHHHHHCCEEEECCCCHHHHHHHHHHHH
LKVPCQVILTSDSQYMRQGITQWIHGWKRKGWLTSAGQPVKNVDLWKRLDTVSQRHQIDW
HHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
RWVKGHAGHTENERCDDLARQAAEAKPSQEDSGYINQQAQA
EEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGLKQISIYTDGSCLGNPGPGGYGIVLKYKKQTKELADGFALTTNNRMELLAPIVALEV
CCCEEEEEEECCCCCCCCCCCCEEEEEEECHHHHHHHCCEEEECCCCHHHHHHHHHHHH
LKVPCQVILTSDSQYMRQGITQWIHGWKRKGWLTSAGQPVKNVDLWKRLDTVSQRHQIDW
HHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
RWVKGHAGHTENERCDDLARQAAEAKPSQEDSGYINQQAQA
EEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA