Click here to switch to the map view.

The map label for this gene is gloB

Identifier: 167623801

GI number: 167623801

Start: 2245112

End: 2245888

Strand: Reverse

Name: gloB

Synonym: Shal_1871

Alternate gene names: 167623801

Gene position: 2245888-2245112 (Counterclockwise)

Preceding gene: 167623804

Following gene: 167623800

Centisome position: 42.97

GC content: 45.56

Gene sequence:

>777_bases
ATGTTACAGATCACTCCACTACCTGCGTTCAACGATAATTATATCTGGGTGTTTCAATCTCAGCAAAGCTCTGGCGTCTA
TGTGGTTGATCCCGGCGACGGCCAAGTAGTCACAGACTATTTAACCCAAACCAATTTGCCGCTACTTGGGATCTTAATTA
CCCATCATCACCATGATCATACGGGGGGGATCGAGCAGCTACTTAAGCTGTTCGGTGAGGACACTCCAGTATATGGTCCA
CAAGTCGAAAATATAGCTGGCGTTAACCGCCCTATCAGCACAAATGGTAACATTACACTAGAGAATATTGGCTTGAATAC
GACAGTCATTCAAGTCCCGGGGCATACATCTGGTCACATCTGCTACTTAATCGAAGACGCGCTATTTTGTGGTGATACCC
TATTCAGTGGAGGCTGCGGCCGCTTATTTGAAGGGACTGCAGAGCAGATGTACCAGTCGTTAACTCAGTTAAGTCTGCTA
CCCGATAATACCCGAGTTTTTTGCGCTCACGAATACACCTTAGCCAATCTTCACTTTGCCAAAGCGGTTGAAGCAAATAA
CCCTGCACTTATCGAGTACACAGTAAAAGCCCATGCCCTGAGAGCGGAAAATAAGCCCACTTTGCCCTCCTCAATAGCCC
TAGAAAAAGCCATAAACCCTTTTTTACGCCTTGATAGCCTAGAAATTCAAAAATCTTTAGCCGTTCAATTCCAACAACCT
ATCGCAGATCCCGTGCAAAGCTTCGCACTTCTGCGTCAATGGAAAGACAATTTCTAA

Upstream 100 bases:

>100_bases
CGAAAGCGGTATACAAGCAACAGCTTTGGTCAGTAAACTTGTTTAAACGCTCAAATAAATTAGATAGAGCGGTTAACTTC
TTTAAATAGAGAGTCTCGAT

Downstream 100 bases:

>100_bases
AAATTATTCTACAATAGTCCGCTATTTCAGCGCCACGAAGCCAGAACTAAGCAAGAGTTTTCTGGCTTTTTTGAGCCTAT
TTTAAGGACACACATTTTCA

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLQITPLPAFNDNYIWVFQSQQSSGVYVVDPGDGQVVTDYLTQTNLPLLGILITHHHHDHTGGIEQLLKLFGEDTPVYGP
QVENIAGVNRPISTNGNITLENIGLNTTVIQVPGHTSGHICYLIEDALFCGDTLFSGGCGRLFEGTAEQMYQSLTQLSLL
PDNTRVFCAHEYTLANLHFAKAVEANNPALIEYTVKAHALRAENKPTLPSSIALEKAINPFLRLDSLEIQKSLAVQFQQP
IADPVQSFALLRQWKDNF

Sequences:

>Translated_258_residues
MLQITPLPAFNDNYIWVFQSQQSSGVYVVDPGDGQVVTDYLTQTNLPLLGILITHHHHDHTGGIEQLLKLFGEDTPVYGP
QVENIAGVNRPISTNGNITLENIGLNTTVIQVPGHTSGHICYLIEDALFCGDTLFSGGCGRLFEGTAEQMYQSLTQLSLL
PDNTRVFCAHEYTLANLHFAKAVEANNPALIEYTVKAHALRAENKPTLPSSIALEKAINPFLRLDSLEIQKSLAVQFQQP
IADPVQSFALLRQWKDNF
>Mature_258_residues
MLQITPLPAFNDNYIWVFQSQQSSGVYVVDPGDGQVVTDYLTQTNLPLLGILITHHHHDHTGGIEQLLKLFGEDTPVYGP
QVENIAGVNRPISTNGNITLENIGLNTTVIQVPGHTSGHICYLIEDALFCGDTLFSGGCGRLFEGTAEQMYQSLTQLSLL
PDNTRVFCAHEYTLANLHFAKAVEANNPALIEYTVKAHALRAENKPTLPSSIALEKAINPFLRLDSLEIQKSLAVQFQQP
IADPVQSFALLRQWKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=265, Percent_Identity=37.7358490566038, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI94538322, Length=265, Percent_Identity=37.7358490566038, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI21703352, Length=240, Percent_Identity=35, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI116642887, Length=240, Percent_Identity=35, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI14150041, Length=237, Percent_Identity=34.1772151898734, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI46361987, Length=193, Percent_Identity=35.7512953367876, Blast_Score=117, Evalue=9e-27,
Organism=Escherichia coli, GI1786406, Length=257, Percent_Identity=43.579766536965, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787158, Length=206, Percent_Identity=28.1553398058252, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=274, Percent_Identity=35.036496350365, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320478, Length=246, Percent_Identity=32.9268292682927, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6324614, Length=236, Percent_Identity=28.8135593220339, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356335, Length=265, Percent_Identity=41.8867924528302, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24667711, Length=265, Percent_Identity=41.8867924528302, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24667703, Length=265, Percent_Identity=41.8867924528302, Blast_Score=208, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_SHEHH (B0TRL8)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674095.1
- ProteinModelPortal:   B0TRL8
- SMR:   B0TRL8
- GeneID:   5904005
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1871
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK982151
- BioCyc:   SHAL458817:SHAL_1871-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28482; Mature: 28482

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQITPLPAFNDNYIWVFQSQQSSGVYVVDPGDGQVVTDYLTQTNLPLLGILITHHHHDH
CEEECCCCCCCCCEEEEEECCCCCCEEEEECCCCEEEEEEHHHCCCCEEEEEEEECCCCC
TGGIEQLLKLFGEDTPVYGPQVENIAGVNRPISTNGNITLENIGLNTTVIQVPGHTSGHI
CCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCEE
CYLIEDALFCGDTLFSGGCGRLFEGTAEQMYQSLTQLSLLPDNTRVFCAHEYTLANLHFA
EEEEECHHHHCCHHHCCCCCHHHCCHHHHHHHHHHHHEECCCCCEEEEEECEEEEEEEEE
KAVEANNPALIEYTVKAHALRAENKPTLPSSIALEKAINPFLRLDSLEIQKSLAVQFQQP
HEECCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHH
IADPVQSFALLRQWKDNF
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLQITPLPAFNDNYIWVFQSQQSSGVYVVDPGDGQVVTDYLTQTNLPLLGILITHHHHDH
CEEECCCCCCCCCEEEEEECCCCCCEEEEECCCCEEEEEEHHHCCCCEEEEEEEECCCCC
TGGIEQLLKLFGEDTPVYGPQVENIAGVNRPISTNGNITLENIGLNTTVIQVPGHTSGHI
CCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCEE
CYLIEDALFCGDTLFSGGCGRLFEGTAEQMYQSLTQLSLLPDNTRVFCAHEYTLANLHFA
EEEEECHHHHCCHHHCCCCCHHHCCHHHHHHHHHHHHEECCCCCEEEEEECEEEEEEEEE
KAVEANNPALIEYTVKAHALRAENKPTLPSSIALEKAINPFLRLDSLEIQKSLAVQFQQP
HEECCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHH
IADPVQSFALLRQWKDNF
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA