Click here to switch to the map view.

The map label for this gene is rnt [H]

Identifier: 167623615

GI number: 167623615

Start: 2019116

End: 2019811

Strand: Reverse

Name: rnt [H]

Synonym: Shal_1684

Alternate gene names: 167623615

Gene position: 2019811-2019116 (Counterclockwise)

Preceding gene: 167623626

Following gene: 167623613

Centisome position: 38.64

GC content: 43.97

Gene sequence:

>696_bases
ATGACAGACACTTTCGACCCTAATTTATTGAAAAACCGCTTTAGAGGGTATTTCCCAGTGGTCATTGATGTTGAAACCGC
AGGTTTTAACGCAAAAACTGATGCGCTATTAGAAATCGCTGTCAGTATGTTAAAGATGGACGATGAAGGTGTTCTCAGTC
TTGATAAGACATTACATTTTAATATTGAGCCGTTCGAAGGGTCAAACCTTGAGCCTGAGGCCTTAGCATTTACAGGTATC
GACCCAACCAGCCCTCTTCGTGGTGCCGTCAGTGAAAAAGAAGCATTCTTAGAGATCTTTAAAGAGGTTAAAAAAGCGCA
AAAAGCCGCAGGCTGTCATCGCAGTATTATTGTTGCTCACAACGCAGCCTTCGATCATGGCTTTGTGACTCAAGCTATTG
AGCGTAATGCGCTTAAGCGCACGCCGTTTCATCCATTTGCAACCTTTGATACAGCTGCTCTTGCAGGTTTAGCAATTGGA
CACACCGTGCTGGCAAAAGCCTGTGCTATGGCGGGAATAGCCTTCGATAATCGTGAAGCACACTCAGCCCTATATGATAC
AGAGCGTACTGCTGAGCTATTTTGCTTAATTGTAAACCGCTGGAAAGCCCTAGGTGGTTGGCCTTTTATTGCTGATGAAG
TAGAAGTAGAAGTTGAAGTTGAAGAAGTGGAACCTCAAGAAACGATTGAGTCTTAA

Upstream 100 bases:

>100_bases
AAAGATTTACCTTTCTTTAGCCTATAAAACAAGCAAACGCACATGACAGTGATTATCAGTTGCAGCATAATAGTCACTTG
CACATTTTTGGGAAATTTTA

Downstream 100 bases:

>100_bases
GCTTTCATAAGGCGATGGTTTCAGACTGTGCACAAATAGTTCTGATACAAAGCGATTAAAATGGCGAACAGAGTATTTAT
GTTCGTCTTTTTTTTAGCCG

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTDTFDPNLLKNRFRGYFPVVIDVETAGFNAKTDALLEIAVSMLKMDDEGVLSLDKTLHFNIEPFEGSNLEPEALAFTGI
DPTSPLRGAVSEKEAFLEIFKEVKKAQKAAGCHRSIIVAHNAAFDHGFVTQAIERNALKRTPFHPFATFDTAALAGLAIG
HTVLAKACAMAGIAFDNREAHSALYDTERTAELFCLIVNRWKALGGWPFIADEVEVEVEVEEVEPQETIES

Sequences:

>Translated_231_residues
MTDTFDPNLLKNRFRGYFPVVIDVETAGFNAKTDALLEIAVSMLKMDDEGVLSLDKTLHFNIEPFEGSNLEPEALAFTGI
DPTSPLRGAVSEKEAFLEIFKEVKKAQKAAGCHRSIIVAHNAAFDHGFVTQAIERNALKRTPFHPFATFDTAALAGLAIG
HTVLAKACAMAGIAFDNREAHSALYDTERTAELFCLIVNRWKALGGWPFIADEVEVEVEVEEVEPQETIES
>Mature_230_residues
TDTFDPNLLKNRFRGYFPVVIDVETAGFNAKTDALLEIAVSMLKMDDEGVLSLDKTLHFNIEPFEGSNLEPEALAFTGID
PTSPLRGAVSEKEAFLEIFKEVKKAQKAAGCHRSIIVAHNAAFDHGFVTQAIERNALKRTPFHPFATFDTAALAGLAIGH
TVLAKACAMAGIAFDNREAHSALYDTERTAELFCLIVNRWKALGGWPFIADEVEVEVEVEEVEPQETIES

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=213, Percent_Identity=69.4835680751174, Blast_Score=303, Evalue=6e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 25413; Mature: 25282

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTFDPNLLKNRFRGYFPVVIDVETAGFNAKTDALLEIAVSMLKMDDEGVLSLDKTLHF
CCCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEE
NIEPFEGSNLEPEALAFTGIDPTSPLRGAVSEKEAFLEIFKEVKKAQKAAGCHRSIIVAH
EECCCCCCCCCCCCEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
NAAFDHGFVTQAIERNALKRTPFHPFATFDTAALAGLAIGHTVLAKACAMAGIAFDNREA
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HSALYDTERTAELFCLIVNRWKALGGWPFIADEVEVEVEVEEVEPQETIES
HHHHHCCHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEEECCCCHHHHCC
>Mature Secondary Structure 
TDTFDPNLLKNRFRGYFPVVIDVETAGFNAKTDALLEIAVSMLKMDDEGVLSLDKTLHF
CCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEE
NIEPFEGSNLEPEALAFTGIDPTSPLRGAVSEKEAFLEIFKEVKKAQKAAGCHRSIIVAH
EECCCCCCCCCCCCEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
NAAFDHGFVTQAIERNALKRTPFHPFATFDTAALAGLAIGHTVLAKACAMAGIAFDNREA
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HSALYDTERTAELFCLIVNRWKALGGWPFIADEVEVEVEVEEVEPQETIES
HHHHHCCHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA