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The map label for this gene is mltF

Identifier: 167623311

GI number: 167623311

Start: 1659565

End: 1660992

Strand: Reverse

Name: mltF

Synonym: Shal_1379

Alternate gene names: 167623311

Gene position: 1660992-1659565 (Counterclockwise)

Preceding gene: 167623316

Following gene: 167623307

Centisome position: 31.78

GC content: 45.17

Gene sequence:

>1428_bases
ATGAAAAAACTGTTACTTATTCTATGCTGTATCACCTTACTGGCTGCCTGCCAGAAGGTTGTCGTGGAACAAGAAGAGCC
AGTCTTTATCCCTGAGCCAACCGAGTTAGTTGTTGGTACGCTATATGGCCCACAGATTTATTTCACCTCAGGTCAGGGCG
ACTCAGGTTACGACTATGAAATGGCAGAGCGGTTCGCTAAATACCTCAATCTTAAATTAAAAATGAGAGCCTTTGCCAAT
ATTAGTGAACTGTATGCAGCAATGCGAAGTGGCGAAATTGATATCATTGCCGCAGGCCTTGGTGATACACCGGCCAGACG
AGAACAGTTTAGGGTTGGCCCACCACTTTATCGCGTTGATCAAGTCTTGGTTTACCGTCAGGGTACCCCTGTACCTAAGA
ACATTAATTCGCTCAACGGTGAAATCACCGTCATTACCGACTCCTCCTTTGTAGATACCTTAACTGATCTACAAAAGTCC
AATCCAGACTTGGTGTGGAACCAAGAAAAAGATAAAGATGCGGAAGAATTACTCACCATGATCGCCGCTGGAGACATCCC
TTATACCATTGCAGATTCAACCAGTTTAGACATCAACAGACGTTTTATGCCTGAACTGCGTGAAGGGTTAGTGCTGAAAA
AGAAACAACCTGTGGTATGGCTGCTCCCTGCGAATAATAGCGACAAGTTAATGAGCCAATTATTGGCATTTTGGCATATA
GAAAAACGTAGCGGCACCTTAGCGCATCTCAATGAGAAATACTTTGCCCATGTAAAACGCTTTGATTATGTCGATACCCG
CGCTTTTATTCGTGCTATCGACAATAAACTACCAAAGTACCAAAGTACCTTCGAGAAGTACGCCGGAGATATTGATTGGC
GCAAGCTCGCGGCGACGGCTTATCAAGAGTCTCATTGGAATCCGAATGCACGTTCACCCACTGGCGTGCGTGGCCTGATG
ATGCTCACCTTGCCCACCGCTAAACAAGTCGGCATTAAAAACCGTCTCGATCCATACCAGAGTATTCAAGGTGGCGCCAA
ATACCTTAATAGCATGCTTGAGCGACTCCCAGAGTCTATTCCAGATAGCCAGCGCATGTGGTTTGCCCTCGCATCCTACA
ATATTGGACTTGGTCATGTTGAAGATGCGCGCAAACTGGCACAGACTCAAGGATTAAATCCGAGTGCATGGCGCGATGTG
AAATCAGTCTTACCACTGCTGCAAAAGCGTAAATACTACAAAAAAACCCGTTACGGTTATGCTCGTGGCAATGAAGCGGT
ACATTATGTCGATAGCATTCGTCGCTACTACGACACCCTAGTGTGGATAGATAACCAGAATATGTTATTAGAATTAAAGA
AAGAAAACATTCAAATGGCAGAAAACCTTGAGCAAAAGACTGAAGCTGCACAACCGCAACAACCTTGA

Upstream 100 bases:

>100_bases
AAACACGCCATTTTTTTACGGCAACCAAGAATGTGTTATAATCGACGGCTATTTCTTTATTTTCTTAATAATTTAAGTTA
AGCGCTGACAAAAATAATCG

Downstream 100 bases:

>100_bases
GCTAGACAGCCTTGAGTCAAACACCCCCGCTCAAAACTCATGCGATAACAAAACCGCAGAATTGAGCGTTTTTCAGGCAA
ACAGCACACTACTTAGCTAG

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKKLLLILCCITLLAACQKVVVEQEEPVFIPEPTELVVGTLYGPQIYFTSGQGDSGYDYEMAERFAKYLNLKLKMRAFAN
ISELYAAMRSGEIDIIAAGLGDTPARREQFRVGPPLYRVDQVLVYRQGTPVPKNINSLNGEITVITDSSFVDTLTDLQKS
NPDLVWNQEKDKDAEELLTMIAAGDIPYTIADSTSLDINRRFMPELREGLVLKKKQPVVWLLPANNSDKLMSQLLAFWHI
EKRSGTLAHLNEKYFAHVKRFDYVDTRAFIRAIDNKLPKYQSTFEKYAGDIDWRKLAATAYQESHWNPNARSPTGVRGLM
MLTLPTAKQVGIKNRLDPYQSIQGGAKYLNSMLERLPESIPDSQRMWFALASYNIGLGHVEDARKLAQTQGLNPSAWRDV
KSVLPLLQKRKYYKKTRYGYARGNEAVHYVDSIRRYYDTLVWIDNQNMLLELKKENIQMAENLEQKTEAAQPQQP

Sequences:

>Translated_475_residues
MKKLLLILCCITLLAACQKVVVEQEEPVFIPEPTELVVGTLYGPQIYFTSGQGDSGYDYEMAERFAKYLNLKLKMRAFAN
ISELYAAMRSGEIDIIAAGLGDTPARREQFRVGPPLYRVDQVLVYRQGTPVPKNINSLNGEITVITDSSFVDTLTDLQKS
NPDLVWNQEKDKDAEELLTMIAAGDIPYTIADSTSLDINRRFMPELREGLVLKKKQPVVWLLPANNSDKLMSQLLAFWHI
EKRSGTLAHLNEKYFAHVKRFDYVDTRAFIRAIDNKLPKYQSTFEKYAGDIDWRKLAATAYQESHWNPNARSPTGVRGLM
MLTLPTAKQVGIKNRLDPYQSIQGGAKYLNSMLERLPESIPDSQRMWFALASYNIGLGHVEDARKLAQTQGLNPSAWRDV
KSVLPLLQKRKYYKKTRYGYARGNEAVHYVDSIRRYYDTLVWIDNQNMLLELKKENIQMAENLEQKTEAAQPQQP
>Mature_475_residues
MKKLLLILCCITLLAACQKVVVEQEEPVFIPEPTELVVGTLYGPQIYFTSGQGDSGYDYEMAERFAKYLNLKLKMRAFAN
ISELYAAMRSGEIDIIAAGLGDTPARREQFRVGPPLYRVDQVLVYRQGTPVPKNINSLNGEITVITDSSFVDTLTDLQKS
NPDLVWNQEKDKDAEELLTMIAAGDIPYTIADSTSLDINRRFMPELREGLVLKKKQPVVWLLPANNSDKLMSQLLAFWHI
EKRSGTLAHLNEKYFAHVKRFDYVDTRAFIRAIDNKLPKYQSTFEKYAGDIDWRKLAATAYQESHWNPNARSPTGVRGLM
MLTLPTAKQVGIKNRLDPYQSIQGGAKYLNSMLERLPESIPDSQRMWFALASYNIGLGHVEDARKLAQTQGLNPSAWRDV
KSVLPLLQKRKYYKKTRYGYARGNEAVHYVDSIRRYYDTLVWIDNQNMLLELKKENIQMAENLEQKTEAAQPQQP

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=443, Percent_Identity=44.2437923250564, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_SHEHH (B0TLJ6)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673605.1
- ProteinModelPortal:   B0TLJ6
- GeneID:   5906549
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1379
- HOGENOM:   HBG644469
- OMA:   QYVENIR
- ProtClustDB:   PRK10859
- BioCyc:   SHAL458817:SHAL_1379-MONOMER
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 54274; Mature: 54274

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00922 TRANSGLYCOSYLASE; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 303-303

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLILCCITLLAACQKVVVEQEEPVFIPEPTELVVGTLYGPQIYFTSGQGDSGYDYE
CHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHEEEEEECCEEEEECCCCCCCCCHH
MAERFAKYLNLKLKMRAFANISELYAAMRSGEIDIIAAGLGDTPARREQFRVGPPLYRVD
HHHHHHHHHCHHEEHHHHHCHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCCCHHHHH
QVLVYRQGTPVPKNINSLNGEITVITDSSFVDTLTDLQKSNPDLVWNQEKDKDAEELLTM
HEEEEECCCCCCCCHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHH
IAAGDIPYTIADSTSLDINRRFMPELREGLVLKKKQPVVWLLPANNSDKLMSQLLAFWHI
HHCCCCCEEEECCCCCCCCHHHHHHHHCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHH
EKRSGTLAHLNEKYFAHVKRFDYVDTRAFIRAIDNKLPKYQSTFEKYAGDIDWRKLAATA
HCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHH
YQESHWNPNARSPTGVRGLMMLTLPTAKQVGIKNRLDPYQSIQGGAKYLNSMLERLPESI
HHHCCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PDSQRMWFALASYNIGLGHVEDARKLAQTQGLNPSAWRDVKSVLPLLQKRKYYKKTRYGY
CCCHHHEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ARGNEAVHYVDSIRRYYDTLVWIDNQNMLLELKKENIQMAENLEQKTEAAQPQQP
CCCCCHHHHHHHHHHHHHEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKLLLILCCITLLAACQKVVVEQEEPVFIPEPTELVVGTLYGPQIYFTSGQGDSGYDYE
CHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHEEEEEECCEEEEECCCCCCCCCHH
MAERFAKYLNLKLKMRAFANISELYAAMRSGEIDIIAAGLGDTPARREQFRVGPPLYRVD
HHHHHHHHHCHHEEHHHHHCHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCCCHHHHH
QVLVYRQGTPVPKNINSLNGEITVITDSSFVDTLTDLQKSNPDLVWNQEKDKDAEELLTM
HEEEEECCCCCCCCHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHH
IAAGDIPYTIADSTSLDINRRFMPELREGLVLKKKQPVVWLLPANNSDKLMSQLLAFWHI
HHCCCCCEEEECCCCCCCCHHHHHHHHCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHH
EKRSGTLAHLNEKYFAHVKRFDYVDTRAFIRAIDNKLPKYQSTFEKYAGDIDWRKLAATA
HCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHH
YQESHWNPNARSPTGVRGLMMLTLPTAKQVGIKNRLDPYQSIQGGAKYLNSMLERLPESI
HHHCCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PDSQRMWFALASYNIGLGHVEDARKLAQTQGLNPSAWRDVKSVLPLLQKRKYYKKTRYGY
CCCHHHEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ARGNEAVHYVDSIRRYYDTLVWIDNQNMLLELKKENIQMAENLEQKTEAAQPQQP
CCCCCHHHHHHHHHHHHHEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA