Click here to switch to the map view.

The map label for this gene is smpB

Identifier: 167623277

GI number: 167623277

Start: 1620316

End: 1620810

Strand: Reverse

Name: smpB

Synonym: Shal_1345

Alternate gene names: 167623277

Gene position: 1620810-1620316 (Counterclockwise)

Preceding gene: 167623280

Following gene: 167623253

Centisome position: 31.01

GC content: 44.04

Gene sequence:

>495_bases
ATGGCTAAGAAAAACGCAAAAAATTCAAAGAACAGTTCAGCTTCAATCGCCCGCAACAAGCGCGCGACCTTTGACTATAA
GTTTGAAGAGAAGCTAGAAGCAGGCCTATCCCTCATGGGATGGGAAGTTAAGTCTATCCGCATGGGCAAGGTTAACTTGT
CTGAGAGCTATGTGTTCCTAAGAGAAGGCGAAGCTTACCTTTTTGGTTGCACTATCGCGCCACTTAATACTGCATCCTCC
CATGTCGTATGTGATCCGCTCCGCTCGCGCAAGCTACTGCTTAAACGTAAAGAGTTGGATAAACTTCAAGGTTTAGTTGA
CCGTAAAGGTTACTCTATTGTCCCAATCTCTATGTATTGGCAAAAAGGTGCATGGGTAAAGATTGAAATTGGCTTGGGTA
AAGGTAAGAAAGAACACGATAAGCGTGACGATACTAAAGATCGTGAATGGCAGATAGAAAAGGCTCGTACCATAAAGAAG
GCGGTACAGCAGTAA

Upstream 100 bases:

>100_bases
GGCTAAAAAGCAAACAAACTCACCTATCACGGTAACAAAACATCGAATAAATCGATATCGCCAATTCCATATTTGTTTAG
CGCGAGTATAATAGCTGCCT

Downstream 100 bases:

>100_bases
TTTCAATAACGTACGTGGTCAATAACGTACGTATAACGAACAATCGATTGCTAATGCGATTTTTATGCTTGGTAAAACAA
GGTAGGTCGCGTTACAATCA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAKKNAKNSKNSSASIARNKRATFDYKFEEKLEAGLSLMGWEVKSIRMGKVNLSESYVFLREGEAYLFGCTIAPLNTASS
HVVCDPLRSRKLLLKRKELDKLQGLVDRKGYSIVPISMYWQKGAWVKIEIGLGKGKKEHDKRDDTKDREWQIEKARTIKK
AVQQ

Sequences:

>Translated_164_residues
MAKKNAKNSKNSSASIARNKRATFDYKFEEKLEAGLSLMGWEVKSIRMGKVNLSESYVFLREGEAYLFGCTIAPLNTASS
HVVCDPLRSRKLLLKRKELDKLQGLVDRKGYSIVPISMYWQKGAWVKIEIGLGKGKKEHDKRDDTKDREWQIEKARTIKK
AVQQ
>Mature_163_residues
AKKNAKNSKNSSASIARNKRATFDYKFEEKLEAGLSLMGWEVKSIRMGKVNLSESYVFLREGEAYLFGCTIAPLNTASSH
VVCDPLRSRKLLLKRKELDKLQGLVDRKGYSIVPISMYWQKGAWVKIEIGLGKGKKEHDKRDDTKDREWQIEKARTIKKA
VQQ

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=161, Percent_Identity=60.2484472049689, Blast_Score=204, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_SHEHH (B0TLG2)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673571.1
- ProteinModelPortal:   B0TLG2
- SMR:   B0TLG2
- GeneID:   5906681
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1345
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   SHAL458817:SHAL_1345-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18780; Mature: 18649

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKNAKNSKNSSASIARNKRATFDYKFEEKLEAGLSLMGWEVKSIRMGKVNLSESYVFL
CCCCCCCCCCCCCCHHHCCCCCEECCCHHHHHHCCCEEECEEEEEEEECEEECCCCEEEE
REGEAYLFGCTIAPLNTASSHVVCDPLRSRKLLLKRKELDKLQGLVDRKGYSIVPISMYW
ECCCEEEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
QKGAWVKIEIGLGKGKKEHDKRDDTKDREWQIEKARTIKKAVQQ
CCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKNAKNSKNSSASIARNKRATFDYKFEEKLEAGLSLMGWEVKSIRMGKVNLSESYVFL
CCCCCCCCCCCCCHHHCCCCCEECCCHHHHHHCCCEEECEEEEEEEECEEECCCCEEEE
REGEAYLFGCTIAPLNTASSHVVCDPLRSRKLLLKRKELDKLQGLVDRKGYSIVPISMYW
ECCCEEEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
QKGAWVKIEIGLGKGKKEHDKRDDTKDREWQIEKARTIKKAVQQ
CCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA