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The map label for this gene is gcvA [H]

Identifier: 167623224

GI number: 167623224

Start: 1571347

End: 1572246

Strand: Reverse

Name: gcvA [H]

Synonym: Shal_1291

Alternate gene names: 167623224

Gene position: 1572246-1571347 (Counterclockwise)

Preceding gene: 167623230

Following gene: 167623220

Centisome position: 30.08

GC content: 34.56

Gene sequence:

>900_bases
ATGAGAATACTTCCACCAATAAAATCCATTATTTACTTTGAATCAGTTGCACGTTTTCAAAGTTTTAAATTGGCTGCTGC
TGAACAAAATGTGACACCGGGAGCTGTAAGCCATCAAATAAACAAACTAGAAGAGTTTATTGGAAAACCTCTATTCATTA
GAGGAAATAGACAAATACAATTATCGAGCTATGGTATTAGATATTACTCTCGGATCTCTATAATCCTCAAGAATATCGAG
GAGGCAACCATTGATCTTGGAATTGAAGGGAAAAGACCACAGATTAGAATCGCTGTACCACCATCTTTACTTAAGAACTG
GTTGTTACCAGTATTAATGAATGACGCTAAATCGAACATCAATAGTAACATCGAATTTATTGACACGTTAGACTTTTTAG
AGTTATCTAAATCAGACTTAGATCTGGCGATTAGATACGGTTATGATACATGGGAAAATATGTACTCGGTTCATTTGTTT
GATGAGACCATGATCCCTGTTTGTCATCCTGACTATCTCAAAGACAAAGGCCAACACTTTGATCAAGAAACTATCGATAA
AAGTACACTTATCTATACCAATAATCGGCTAGTGCAATGGGATATTGTATTGCAAAGACTAAATTTATTAAGTAACAATT
TCACACAACAAAAAATTACCTTTCAGAACTCAATCCAATCTATTGAAGCTGCAAAGCAAGGTGGCGGGATTGCCTATGTT
AATAGCATATTAGTACAGAAAGAGTTGAATAATGGACGGTTAATTCAACCTTTCGATATAGAGATCCCAGAACATAAACC
TCCCTCTTATTACTTAGTTACAACTTATGAAAAGATGCAGCAAGAATTTACTGCTAAAATGTACTATACCATATTGAAAC
ACTGTAAACAGAGCGTTTAA

Upstream 100 bases:

>100_bases
AATAACTAAACCGGCAGAGCAATTACATTGACTAATAAATGTAAACTGACAACTTAGTTTAACTAGACTGAAAGTTTGTA
TATTTAAAAGGAACAAATAA

Downstream 100 bases:

>100_bases
ACTAGTTCTGTTACAATGTACTTACGTTAAAAGAATAATAAAACCAACTAGTTAATCATAGTTATATGTGTAGTAGCTCA
GACTTTATCTGACAGTTACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRILPPIKSIIYFESVARFQSFKLAAAEQNVTPGAVSHQINKLEEFIGKPLFIRGNRQIQLSSYGIRYYSRISIILKNIE
EATIDLGIEGKRPQIRIAVPPSLLKNWLLPVLMNDAKSNINSNIEFIDTLDFLELSKSDLDLAIRYGYDTWENMYSVHLF
DETMIPVCHPDYLKDKGQHFDQETIDKSTLIYTNNRLVQWDIVLQRLNLLSNNFTQQKITFQNSIQSIEAAKQGGGIAYV
NSILVQKELNNGRLIQPFDIEIPEHKPPSYYLVTTYEKMQQEFTAKMYYTILKHCKQSV

Sequences:

>Translated_299_residues
MRILPPIKSIIYFESVARFQSFKLAAAEQNVTPGAVSHQINKLEEFIGKPLFIRGNRQIQLSSYGIRYYSRISIILKNIE
EATIDLGIEGKRPQIRIAVPPSLLKNWLLPVLMNDAKSNINSNIEFIDTLDFLELSKSDLDLAIRYGYDTWENMYSVHLF
DETMIPVCHPDYLKDKGQHFDQETIDKSTLIYTNNRLVQWDIVLQRLNLLSNNFTQQKITFQNSIQSIEAAKQGGGIAYV
NSILVQKELNNGRLIQPFDIEIPEHKPPSYYLVTTYEKMQQEFTAKMYYTILKHCKQSV
>Mature_299_residues
MRILPPIKSIIYFESVARFQSFKLAAAEQNVTPGAVSHQINKLEEFIGKPLFIRGNRQIQLSSYGIRYYSRISIILKNIE
EATIDLGIEGKRPQIRIAVPPSLLKNWLLPVLMNDAKSNINSNIEFIDTLDFLELSKSDLDLAIRYGYDTWENMYSVHLF
DETMIPVCHPDYLKDKGQHFDQETIDKSTLIYTNNRLVQWDIVLQRLNLLSNNFTQQKITFQNSIQSIEAAKQGGGIAYV
NSILVQKELNNGRLIQPFDIEIPEHKPPSYYLVTTYEKMQQEFTAKMYYTILKHCKQSV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=274, Percent_Identity=33.5766423357664, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788706, Length=269, Percent_Identity=29.368029739777, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=26.7716535433071, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI145693193, Length=260, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=255, Percent_Identity=26.2745098039216, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=275, Percent_Identity=24.3636363636364, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790783, Length=256, Percent_Identity=27.734375, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34601; Mature: 34601

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILPPIKSIIYFESVARFQSFKLAAAEQNVTPGAVSHQINKLEEFIGKPLFIRGNRQIQ
CCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCEEE
LSSYGIRYYSRISIILKNIEEATIDLGIEGKRPQIRIAVPPSLLKNWLLPVLMNDAKSNI
EECCCHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCC
NSNIEFIDTLDFLELSKSDLDLAIRYGYDTWENMYSVHLFDETMIPVCHPDYLKDKGQHF
CCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCEEEEEEECCCCCCCCCCHHHHHCCCCC
DQETIDKSTLIYTNNRLVQWDIVLQRLNLLSNNFTQQKITFQNSIQSIEAAKQGGGIAYV
CHHHCCCCEEEEECCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEHH
NSILVQKELNNGRLIQPFDIEIPEHKPPSYYLVTTYEKMQQEFTAKMYYTILKHCKQSV
HHHHHHEECCCCCEECEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRILPPIKSIIYFESVARFQSFKLAAAEQNVTPGAVSHQINKLEEFIGKPLFIRGNRQIQ
CCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCEEE
LSSYGIRYYSRISIILKNIEEATIDLGIEGKRPQIRIAVPPSLLKNWLLPVLMNDAKSNI
EECCCHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCC
NSNIEFIDTLDFLELSKSDLDLAIRYGYDTWENMYSVHLFDETMIPVCHPDYLKDKGQHF
CCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCEEEEEEECCCCCCCCCCHHHHHCCCCC
DQETIDKSTLIYTNNRLVQWDIVLQRLNLLSNNFTQQKITFQNSIQSIEAAKQGGGIAYV
CHHHCCCCEEEEECCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEHH
NSILVQKELNNGRLIQPFDIEIPEHKPPSYYLVTTYEKMQQEFTAKMYYTILKHCKQSV
HHHHHHEECCCCCEECEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]