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The map label for this gene is pcm

Identifier: 167623162

GI number: 167623162

Start: 1492049

End: 1492684

Strand: Direct

Name: pcm

Synonym: Shal_1228

Alternate gene names: 167623162

Gene position: 1492049-1492684 (Clockwise)

Preceding gene: 167623161

Following gene: 167623163

Centisome position: 28.55

GC content: 46.86

Gene sequence:

>636_bases
ATGACCCCAGTTGCCTCAACATCAGCATTGAACCTTGCAAGAAGTCTGTATGAGGCTGGGATCCGCAATGAGGCAGTATT
ACAGGCTGTGGCAAACACTCCGAGGGAGCAATTTCTTGATGCAGCTCTGGGTCACAAAGCTTATGAAAATACGGCGCTAC
CTATTGGGCAGGGACAAACTATTTCTCAGCCCTACATTGTTGCCAAAATGACTGAGATCATCTTGCAAACTAAGCCCACT
AAAGTATTGGAGATAGGAACGGGCTCAGGGTATCAAGCCGCTATTCTCGCACGGTTAGTGCCACAACTCTTTACGGTGGA
GCGGATCAAGAGTTTACAGATCCAAGCTAGGCAGAGATTGAAAAGACTCGATCTGCATAATATCGCTTTTAAATATGGCG
ATGGCTGGGAAGGGTGGCCGAGCAAAGGACCTTATGATGCGATCATGGTTACTGCTGCAGCGAGTTCAATCCCTGATGCT
CTAGTATCTCAATTAGCAGATGGTGGCATATTAGTGATCCCTGTAGGGGAAATCGCTCAGCAGTTATTAAAAGTGACACG
CAGTGGTGATAAATACAGGTCTGAAGTTGTAGAAGATGTACGCTTCGTACCGCTTATCAATGGTGAGCTGGCATAG

Upstream 100 bases:

>100_bases
TCACGCACGACCCGGTTTACGAGGGTCGTGCAGAGTTAACGAATAGCTCCGTGACAGTATCACCACTATTCGAATAAATT
AAATATTGGAAAACAGAAAT

Downstream 100 bases:

>100_bases
TTTTATTCATAAACTGATAAATATAAAACAGTGAAATAGGTTGATTTGGGGTGATGCGTTAATCATGAGGTTGGTTTGCT
TTTTAAGTGTCTGCATAACA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTPVASTSALNLARSLYEAGIRNEAVLQAVANTPREQFLDAALGHKAYENTALPIGQGQTISQPYIVAKMTEIILQTKPT
KVLEIGTGSGYQAAILARLVPQLFTVERIKSLQIQARQRLKRLDLHNIAFKYGDGWEGWPSKGPYDAIMVTAAASSIPDA
LVSQLADGGILVIPVGEIAQQLLKVTRSGDKYRSEVVEDVRFVPLINGELA

Sequences:

>Translated_211_residues
MTPVASTSALNLARSLYEAGIRNEAVLQAVANTPREQFLDAALGHKAYENTALPIGQGQTISQPYIVAKMTEIILQTKPT
KVLEIGTGSGYQAAILARLVPQLFTVERIKSLQIQARQRLKRLDLHNIAFKYGDGWEGWPSKGPYDAIMVTAAASSIPDA
LVSQLADGGILVIPVGEIAQQLLKVTRSGDKYRSEVVEDVRFVPLINGELA
>Mature_210_residues
TPVASTSALNLARSLYEAGIRNEAVLQAVANTPREQFLDAALGHKAYENTALPIGQGQTISQPYIVAKMTEIILQTKPTK
VLEIGTGSGYQAAILARLVPQLFTVERIKSLQIQARQRLKRLDLHNIAFKYGDGWEGWPSKGPYDAIMVTAAASSIPDAL
VSQLADGGILVIPVGEIAQQLLKVTRSGDKYRSEVVEDVRFVPLINGELA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=204, Percent_Identity=28.921568627451, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1789100, Length=200, Percent_Identity=59.5, Blast_Score=243, Evalue=8e-66,
Organism=Caenorhabditis elegans, GI71983477, Length=178, Percent_Identity=31.4606741573034, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=139, Percent_Identity=33.0935251798561, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17981723, Length=182, Percent_Identity=33.5164835164835, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_SHEHH (B0TK10)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673456.1
- ProteinModelPortal:   B0TK10
- SMR:   B0TK10
- GeneID:   5903916
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1228
- HOGENOM:   HBG699907
- OMA:   MEEVPRE
- ProtClustDB:   CLSK907169
- BioCyc:   SHAL458817:SHAL_1228-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 22867; Mature: 22735

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVASTSALNLARSLYEAGIRNEAVLQAVANTPREQFLDAALGHKAYENTALPIGQGQT
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCCCCCCCCC
ISQPYIVAKMTEIILQTKPTKVLEIGTGSGYQAAILARLVPQLFTVERIKSLQIQARQRL
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRLDLHNIAFKYGDGWEGWPSKGPYDAIMVTAAASSIPDALVSQLADGGILVIPVGEIAQ
HHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCEEEEEHHHHHH
QLLKVTRSGDKYRSEVVEDVRFVPLINGELA
HHHHHHHCCHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
TPVASTSALNLARSLYEAGIRNEAVLQAVANTPREQFLDAALGHKAYENTALPIGQGQT
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCCCCCCCCC
ISQPYIVAKMTEIILQTKPTKVLEIGTGSGYQAAILARLVPQLFTVERIKSLQIQARQRL
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRLDLHNIAFKYGDGWEGWPSKGPYDAIMVTAAASSIPDALVSQLADGGILVIPVGEIAQ
HHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCEEEEEHHHHHH
QLLKVTRSGDKYRSEVVEDVRFVPLINGELA
HHHHHHHCCHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA