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The map label for this gene is ispD

Identifier: 167623158

GI number: 167623158

Start: 1488829

End: 1489521

Strand: Direct

Name: ispD

Synonym: Shal_1224

Alternate gene names: 167623158

Gene position: 1488829-1489521 (Clockwise)

Preceding gene: 167623157

Following gene: 167623159

Centisome position: 28.48

GC content: 45.89

Gene sequence:

>693_bases
ATGAGCCAAACACCCGAGCAAATTATTGCGATTGTTCCCGCTGCCGGAATTGGTAGTCGTATGGGCGCAGAAATTCCTAA
GCAGTATTTACAGCTTAATGAACAGAGTATTTTGAGCCACACCTTAGATTGCCTTCTTTCGCATCCCGATATCGATAAGG
TGATCGTCGCACTTAATCCTGCTGATAACTATTTTGCTAAATTGCCTCAAGTGAAACATCCAAAGCTTGAGTGTGTCATT
GGCGGTAAGGAACGTGCTGATTCTGTTTTATCAGGATTAAAAATTGCAGAGGCCGGTGCTTGGGCATTGGTGCATGACGC
TGCGCGGCCATGTCTAACACATAGAGATATTGATAAGCTTATTGCGTCAGTTAATGAGTTCCCACAGGGGGCTATTCTAG
CTGCGCCAGTGCGTGACACGATGAAACGCACCGATGAGAAAGGCTTAATTAGCGAGACGGTTTGCCGAGAAAGACTATGG
CATGCATTAACGCCGCAGTATTTTCCAGTTTCAAGTTTGATGCAAAATTTAACTGATGCGTTAGCGGCTGGTGCGCTTAT
CACTGATGAGGCATCTGCTATGGAGTGGGCCGGAGTTATGCCCGGGATTGTCTCTGGACGTGCCGATAACATTAAAGTGA
CTCATCCAGATGATCTTCAGCTTGCGTCACTCTTTCTCAAAAATGCAGTTTAA

Upstream 100 bases:

>100_bases
TGACAAACCATCAAATGATTAATCTCCATTGATTGTTTGTGTCATCATTTAAGTCCAACGCCGATTAGAGAAAGATGTCG
CTATTACATGGAGCTCTATC

Downstream 100 bases:

>100_bases
CAAGCTACTATTGTAACCCATTGAATAACAGGAACACCGATGAAAATTAGAATAGGTCACGGGTTTGACGTACACAAGTT
TGGTGGTGAGCCGCCACTTA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MSQTPEQIIAIVPAAGIGSRMGAEIPKQYLQLNEQSILSHTLDCLLSHPDIDKVIVALNPADNYFAKLPQVKHPKLECVI
GGKERADSVLSGLKIAEAGAWALVHDAARPCLTHRDIDKLIASVNEFPQGAILAAPVRDTMKRTDEKGLISETVCRERLW
HALTPQYFPVSSLMQNLTDALAAGALITDEASAMEWAGVMPGIVSGRADNIKVTHPDDLQLASLFLKNAV

Sequences:

>Translated_230_residues
MSQTPEQIIAIVPAAGIGSRMGAEIPKQYLQLNEQSILSHTLDCLLSHPDIDKVIVALNPADNYFAKLPQVKHPKLECVI
GGKERADSVLSGLKIAEAGAWALVHDAARPCLTHRDIDKLIASVNEFPQGAILAAPVRDTMKRTDEKGLISETVCRERLW
HALTPQYFPVSSLMQNLTDALAAGALITDEASAMEWAGVMPGIVSGRADNIKVTHPDDLQLASLFLKNAV
>Mature_229_residues
SQTPEQIIAIVPAAGIGSRMGAEIPKQYLQLNEQSILSHTLDCLLSHPDIDKVIVALNPADNYFAKLPQVKHPKLECVIG
GKERADSVLSGLKIAEAGAWALVHDAARPCLTHRDIDKLIASVNEFPQGAILAAPVRDTMKRTDEKGLISETVCRERLWH
ALTPQYFPVSSLMQNLTDALAAGALITDEASAMEWAGVMPGIVSGRADNIKVTHPDDLQLASLFLKNAV

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=25.4166666666667, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1789104, Length=231, Percent_Identity=53.6796536796537, Blast_Score=247, Evalue=6e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_SHEHH (B0TK06)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673452.1
- ProteinModelPortal:   B0TK06
- SMR:   B0TK06
- GeneID:   5903876
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1224
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- ProtClustDB:   PRK00155
- BioCyc:   SHAL458817:SHAL_1224-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 24860; Mature: 24729

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTPEQIIAIVPAAGIGSRMGAEIPKQYLQLNEQSILSHTLDCLLSHPDIDKVIVALNP
CCCCHHHHEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECC
ADNYFAKLPQVKHPKLECVIGGKERADSVLSGLKIAEAGAWALVHDAARPCLTHRDIDKL
CHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
IASVNEFPQGAILAAPVRDTMKRTDEKGLISETVCRERLWHALTPQYFPVSSLMQNLTDA
HHHHHHCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LAAGALITDEASAMEWAGVMPGIVSGRADNIKVTHPDDLQLASLFLKNAV
HHHCCHHCCCHHHHHHHHCCCHHHCCCCCCEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQTPEQIIAIVPAAGIGSRMGAEIPKQYLQLNEQSILSHTLDCLLSHPDIDKVIVALNP
CCCHHHHEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECC
ADNYFAKLPQVKHPKLECVIGGKERADSVLSGLKIAEAGAWALVHDAARPCLTHRDIDKL
CHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
IASVNEFPQGAILAAPVRDTMKRTDEKGLISETVCRERLWHALTPQYFPVSSLMQNLTDA
HHHHHHCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LAAGALITDEASAMEWAGVMPGIVSGRADNIKVTHPDDLQLASLFLKNAV
HHHCCHHCCCHHHHHHHHCCCHHHCCCCCCEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA