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The map label for this gene is ispF

Identifier: 167623159

GI number: 167623159

Start: 1489561

End: 1490040

Strand: Direct

Name: ispF

Synonym: Shal_1225

Alternate gene names: 167623159

Gene position: 1489561-1490040 (Clockwise)

Preceding gene: 167623158

Following gene: 167623160

Centisome position: 28.5

GC content: 45.42

Gene sequence:

>480_bases
ATGAAAATTAGAATAGGTCACGGGTTTGACGTACACAAGTTTGGTGGTGAGCCGCCACTTATTCTCGGCGGTGTCGATGT
GCCTTATGAAGTGGGCTTAATCGCCCATTCTGATGGAGATGTCGCCCTGCACGCAATCTCTGATGCGATTCTTGGGGCGA
TGGCTCTGGGCGATATTGGCAAACATTTCCCAGATACAGACCCAGAGTTTAAAGGTGCCGATAGTCGTGTACTCTTAAGG
CATTGCTATCAGTTAGCCACAGATATGGGCTTTAGCTTATCTAACTTAGATGTCACTATTATTGCTCAGGCGCCGAAAAT
GGCTCCTCATATTGAAGCTATCCGCAAAGTATTGGCGGCAGATTTAGTTGCAGATATCAATGATATCAATGTTAAGGCGA
CAACAACGGAAAAACTTGGCTTTACAGGCAGAAAAGAAGGCATAGCAGTTGAAGCTGTGGTGTTGATGATAAAAGCCTAG

Upstream 100 bases:

>100_bases
TAAAGTGACTCATCCAGATGATCTTCAGCTTGCGTCACTCTTTCTCAAAAATGCAGTTTAACAAGCTACTATTGTAACCC
ATTGAATAACAGGAACACCG

Downstream 100 bases:

>100_bases
CCAATCGTGTGGGCAAGAAGCTATAGCTAGCAATAGTGGCAATAGATTTTTATGAGATAGCAAGATACTTTAGCGAGACA
ATTTAGCGATATGAGTGAAT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKIRIGHGFDVHKFGGEPPLILGGVDVPYEVGLIAHSDGDVALHAISDAILGAMALGDIGKHFPDTDPEFKGADSRVLLR
HCYQLATDMGFSLSNLDVTIIAQAPKMAPHIEAIRKVLAADLVADINDINVKATTTEKLGFTGRKEGIAVEAVVLMIKA

Sequences:

>Translated_159_residues
MKIRIGHGFDVHKFGGEPPLILGGVDVPYEVGLIAHSDGDVALHAISDAILGAMALGDIGKHFPDTDPEFKGADSRVLLR
HCYQLATDMGFSLSNLDVTIIAQAPKMAPHIEAIRKVLAADLVADINDINVKATTTEKLGFTGRKEGIAVEAVVLMIKA
>Mature_159_residues
MKIRIGHGFDVHKFGGEPPLILGGVDVPYEVGLIAHSDGDVALHAISDAILGAMALGDIGKHFPDTDPEFKGADSRVLLR
HCYQLATDMGFSLSNLDVTIIAQAPKMAPHIEAIRKVLAADLVADINDINVKATTTEKLGFTGRKEGIAVEAVVLMIKA

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=70.0636942675159, Blast_Score=204, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_SHEHH (B0TK07)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673453.1
- ProteinModelPortal:   B0TK07
- SMR:   B0TK07
- GeneID:   5903877
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1225
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   SHAL458817:SHAL_1225-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16893; Mature: 16893

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRIGHGFDVHKFGGEPPLILGGVDVPYEVGLIAHSDGDVALHAISDAILGAMALGDIG
CEEECCCCCCHHCCCCCCCEEECCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHH
KHFPDTDPEFKGADSRVLLRHCYQLATDMGFSLSNLDVTIIAQAPKMAPHIEAIRKVLAA
HCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHH
DLVADINDINVKATTTEKLGFTGRKEGIAVEAVVLMIKA
HHHHCCCCCEEEEECHHHCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MKIRIGHGFDVHKFGGEPPLILGGVDVPYEVGLIAHSDGDVALHAISDAILGAMALGDIG
CEEECCCCCCHHCCCCCCCEEECCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHH
KHFPDTDPEFKGADSRVLLRHCYQLATDMGFSLSNLDVTIIAQAPKMAPHIEAIRKVLAA
HCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHH
DLVADINDINVKATTTEKLGFTGRKEGIAVEAVVLMIKA
HHHHCCCCCEEEEECHHHCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA