Click here to switch to the map view.

The map label for this gene is glyA

Identifier: 167623137

GI number: 167623137

Start: 1463160

End: 1464416

Strand: Direct

Name: glyA

Synonym: Shal_1203

Alternate gene names: 167623137

Gene position: 1463160-1464416 (Clockwise)

Preceding gene: 167623129

Following gene: 167623138

Centisome position: 27.99

GC content: 47.49

Gene sequence:

>1257_bases
ATGCTGAAAAAAGATATGAATATCGCGGATTACGATCCACAACTATTTGCAGCAATTGAAGATGAAACACGCCGTCAAGA
AGAGCACATCGAACTCATTGCTTCAGAAAACTACACCAGTCCTCGCGTTCTTGAAGCCCAAGGTACGCAGCTAACCAATA
AGTATGCTGAAGGTTACCCGGGTAAGCGTTACTATGGCGGTTGTGAGCATGTAGATATTGTTGAAGAGCTAGCGATTTCA
CGTGCTAAAGAGCTATTTGGTGCAACTTATGCCAACGTTCAGCCACACTCTGGCTCACAAGCTAACGCAGCGGTATTTAT
GGCGCTGCTTCAAGGTGGCGACACCGTTCTAGGTATGAGCCTAGCACACGGTGGTCACTTAACTCACGGTTCTCATGTGA
GTTTCTCTGGCAAGCTATATAACGCAGTTCAGTACGGTATTGATGAAACAACCGGCAAGATTGATTACGCCGAAGTTGAG
CGTCTAGCGGTTGAGCACAAGCCAAAGATGATCATCGCTGGTTTCAGTGCATATTCAGGCATTGTTGACTGGGGTAAGTT
CCGCGAAATCGCTGATAAAGTAGGTGCATACCTGTTTGTTGATATGGCGCACGTTGCAGGTCTTGTTGCTGCAGGCATCT
ATCCAAGCCCAATGCCACACGCTCACGTTGTGACAACAACGACGCATAAGACACTCGCTGGCCCTCGTGGCGGCTTGATC
TTATCTGCGATTAACGATGAAGATATCTATAAGAAGTTAAACTCAGCGGTCTTCCCAGGCGGTCAGGGTGGTCCATTGAT
GCATGTTATCGCGGCTAAAGCGGTTGCATTTAAAGAGGCGCTAGATCCAGAGTTCACCACTTACCAAGAGCAAGTGGTTG
TTAACGCAAAGGCGATGGCACGCACTTTCATCGAGCGTGGCTACGATGTGGTATCTGGCGGCACTGACAACCACCTATTC
TTGCTTGATCTAATCAGCAAAGATATTACTGGTAAAGATGCTGATGCAGCTCTAGGTAATGCGAACATCACAGTGAACAA
GAACTCTGTACCTAATGACCCACGTTCACCATTTGTGACTTCAGGTCTTCGTATCGGTTCTCCTGCGATTACCCGTCGTG
GCTTTGGTGAAGAAGAGTCTGTGCAACTCACTCACTGGATGTGCGATATCTTAGATGATATTTCAGATCTTGCCGTTAGC
GAGCGTGTTAAGGCTCAGGTTCTGGAATTGTGCGCTAGGTTCCCTGTTTACGGTTAA

Upstream 100 bases:

>100_bases
AAATGCAAGTAATTTCATGTTGTAAATGCTGGGATTGTGATCAAATGCGCTGTAGAATACCGCGCAACCCTATTACAAGA
CTTTACAGCTGGAGTGAGTA

Downstream 100 bases:

>100_bases
CCTTAAGGGTTAAATCAGGTAGACTCCAATGGCCGCATTTATGCGGCCATTTTTGTTCTTATTCGAGTATACATTTGATT
CGAGCATACTTTCTGAACTG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLKKDMNIADYDPQLFAAIEDETRRQEEHIELIASENYTSPRVLEAQGTQLTNKYAEGYPGKRYYGGCEHVDIVEELAIS
RAKELFGATYANVQPHSGSQANAAVFMALLQGGDTVLGMSLAHGGHLTHGSHVSFSGKLYNAVQYGIDETTGKIDYAEVE
RLAVEHKPKMIIAGFSAYSGIVDWGKFREIADKVGAYLFVDMAHVAGLVAAGIYPSPMPHAHVVTTTTHKTLAGPRGGLI
LSAINDEDIYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEALDPEFTTYQEQVVVNAKAMARTFIERGYDVVSGGTDNHLF
LLDLISKDITGKDADAALGNANITVNKNSVPNDPRSPFVTSGLRIGSPAITRRGFGEEESVQLTHWMCDILDDISDLAVS
ERVKAQVLELCARFPVYG

Sequences:

>Translated_418_residues
MLKKDMNIADYDPQLFAAIEDETRRQEEHIELIASENYTSPRVLEAQGTQLTNKYAEGYPGKRYYGGCEHVDIVEELAIS
RAKELFGATYANVQPHSGSQANAAVFMALLQGGDTVLGMSLAHGGHLTHGSHVSFSGKLYNAVQYGIDETTGKIDYAEVE
RLAVEHKPKMIIAGFSAYSGIVDWGKFREIADKVGAYLFVDMAHVAGLVAAGIYPSPMPHAHVVTTTTHKTLAGPRGGLI
LSAINDEDIYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEALDPEFTTYQEQVVVNAKAMARTFIERGYDVVSGGTDNHLF
LLDLISKDITGKDADAALGNANITVNKNSVPNDPRSPFVTSGLRIGSPAITRRGFGEEESVQLTHWMCDILDDISDLAVS
ERVKAQVLELCARFPVYG
>Mature_418_residues
MLKKDMNIADYDPQLFAAIEDETRRQEEHIELIASENYTSPRVLEAQGTQLTNKYAEGYPGKRYYGGCEHVDIVEELAIS
RAKELFGATYANVQPHSGSQANAAVFMALLQGGDTVLGMSLAHGGHLTHGSHVSFSGKLYNAVQYGIDETTGKIDYAEVE
RLAVEHKPKMIIAGFSAYSGIVDWGKFREIADKVGAYLFVDMAHVAGLVAAGIYPSPMPHAHVVTTTTHKTLAGPRGGLI
LSAINDEDIYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEALDPEFTTYQEQVVVNAKAMARTFIERGYDVVSGGTDNHLF
LLDLISKDITGKDADAALGNANITVNKNSVPNDPRSPFVTSGLRIGSPAITRRGFGEEESVQLTHWMCDILDDISDLAVS
ERVKAQVLELCARFPVYG

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=424, Percent_Identity=45.0471698113208, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI261862352, Length=424, Percent_Identity=45.0471698113208, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI261862350, Length=424, Percent_Identity=45.0471698113208, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI261862348, Length=424, Percent_Identity=45.0471698113208, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI261862346, Length=424, Percent_Identity=44.5754716981132, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=45.3865336658354, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=43.4447300771208, Blast_Score=286, Evalue=2e-77,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=77.6978417266187, Blast_Score=666, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=393, Percent_Identity=46.5648854961832, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI25144729, Length=393, Percent_Identity=46.5648854961832, Blast_Score=342, Evalue=3e-94,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=45.3865336658354, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=45.273631840796, Blast_Score=345, Evalue=5e-96,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=46.5, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=46.5, Blast_Score=351, Evalue=5e-97,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_SHEHH (B0TJY5)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673431.1
- ProteinModelPortal:   B0TJY5
- SMR:   B0TJY5
- GeneID:   5906246
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1203
- HOGENOM:   HBG301263
- OMA:   KAMARTF
- ProtClustDB:   PRK00011
- BioCyc:   SHAL458817:SHAL_1203-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45291; Mature: 45291

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKDMNIADYDPQLFAAIEDETRRQEEHIELIASENYTSPRVLEAQGTQLTNKYAEGYP
CCCCCCCCCCCCCHHEEEHHHHHHHHHHHHEEEECCCCCCCEEEECCCCHHHHHHHCCCC
GKRYYGGCEHVDIVEELAISRAKELFGATYANVQPHSGSQANAAVFMALLQGGDTVLGMS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEE
LAHGGHLTHGSHVSFSGKLYNAVQYGIDETTGKIDYAEVERLAVEHKPKMIIAGFSAYSG
ECCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHC
IVDWGKFREIADKVGAYLFVDMAHVAGLVAAGIYPSPMPHAHVVTTTTHKTLAGPRGGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHCCCCCCEE
LSAINDEDIYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEALDPEFTTYQEQVVVNAKAMA
EEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHEEHHHHHH
RTFIERGYDVVSGGTDNHLFLLDLISKDITGKDADAALGNANITVNKNSVPNDPRSPFVT
HHHHHCCCEEECCCCCCCEEEEEEHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHH
SGLRIGSPAITRRGFGEEESVQLTHWMCDILDDISDLAVSERVKAQVLELCARFPVYG
CCEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKKDMNIADYDPQLFAAIEDETRRQEEHIELIASENYTSPRVLEAQGTQLTNKYAEGYP
CCCCCCCCCCCCCHHEEEHHHHHHHHHHHHEEEECCCCCCCEEEECCCCHHHHHHHCCCC
GKRYYGGCEHVDIVEELAISRAKELFGATYANVQPHSGSQANAAVFMALLQGGDTVLGMS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEE
LAHGGHLTHGSHVSFSGKLYNAVQYGIDETTGKIDYAEVERLAVEHKPKMIIAGFSAYSG
ECCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHC
IVDWGKFREIADKVGAYLFVDMAHVAGLVAAGIYPSPMPHAHVVTTTTHKTLAGPRGGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHCCCCCCEE
LSAINDEDIYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEALDPEFTTYQEQVVVNAKAMA
EEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHEEHHHHHH
RTFIERGYDVVSGGTDNHLFLLDLISKDITGKDADAALGNANITVNKNSVPNDPRSPFVT
HHHHHCCCEEECCCCCCCEEEEEEHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHH
SGLRIGSPAITRRGFGEEESVQLTHWMCDILDDISDLAVSERVKAQVLELCARFPVYG
CCEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA