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The map label for this gene is mscS [H]

Identifier: 167623119

GI number: 167623119

Start: 1436528

End: 1437352

Strand: Direct

Name: mscS [H]

Synonym: Shal_1185

Alternate gene names: 167623119

Gene position: 1436528-1437352 (Clockwise)

Preceding gene: 167623118

Following gene: 167623121

Centisome position: 27.48

GC content: 45.33

Gene sequence:

>825_bases
ATGGATAACTTGCAAGGTTTAATGGAGCAAGCACCAGAGTTAATCGTCACCTACGGCATGAAAGTACTGTTTGCGATTAT
CATATTTGTCATCGGTAAGTTCCTGTCGAAAGTCGCAAAAAAAATAACGACAAAAATACTCAACAAACGTAAGGTCGATA
CTACCGTTGTCTCGTTTGTTGCCAATATTGCTTGGGCATTGGTGTTTGTATTTACCATAGTGGCAACCCTTGGCCAAATT
GGTGTGCAAACCGCATCGTTAGTGGCTGTAATTGGTGCTGCGGGCTTGGCAGTTGGCTTAGCGTTACAAGGTTCACTGTC
TAACTTTGCTGCAGGCGTATTGATGGTGCTATTTAGACCATGCCGAGTCGGCGACTATGTGGAAGCTGCTGGGATTGCGG
GGACGGTTGATGAAATCACAATCTTCTCAACGCGCTTACTGACTCCAGATAATAAGCTTGTTATTGCACCCAACTCAGCC
ATGATGAATGGCACAATCGTTAACTATTCAGCCATGGAGACGCGTCGCTTAGATTTGGTGATTGGGGTTTCTTATGATGC
GAACTTAGCCGAGACCAAGAAGGTATTAACTCACATCCTCGATAACAGTCAGTATGTACTCAAAGACCCGGCCTACACAG
TGGCTGTATCAGAATTGGCTGATTCGTCGGTGAACTTTGTGGTTCGCCCTTGGGTTAAAGGCGCAGATTACTGGCCAGCG
CACTTTGAAATTCTAGAGCAAATTAAGAATGCCTTAGATGAAGCGGGAATTGGTATTCCATATCCGCAGATGGATCTCTA
TGTAAAAGAGACGCCAGCGGCTTAA

Upstream 100 bases:

>100_bases
TCGCCGATAACCGAACTCGACAGGGGCGAGAGCAGAATCGTCGAGTCGAGTTTTTAGTTATTGTTCAATAGTTTTATCTT
AAATTTGAGGGAGTATATAA

Downstream 100 bases:

>100_bases
GTTGAGCCATCTTCACTGGTCAGTAGGTAATAAAAAAGGTCGGCATATGCCGACCTTTTTCTCTCTTTCAAACTAGCATA
TTTAAAACTAGCTTAATGCG

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MDNLQGLMEQAPELIVTYGMKVLFAIIIFVIGKFLSKVAKKITTKILNKRKVDTTVVSFVANIAWALVFVFTIVATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRPCRVGDYVEAAGIAGTVDEITIFSTRLLTPDNKLVIAPNSA
MMNGTIVNYSAMETRRLDLVIGVSYDANLAETKKVLTHILDNSQYVLKDPAYTVAVSELADSSVNFVVRPWVKGADYWPA
HFEILEQIKNALDEAGIGIPYPQMDLYVKETPAA

Sequences:

>Translated_274_residues
MDNLQGLMEQAPELIVTYGMKVLFAIIIFVIGKFLSKVAKKITTKILNKRKVDTTVVSFVANIAWALVFVFTIVATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRPCRVGDYVEAAGIAGTVDEITIFSTRLLTPDNKLVIAPNSA
MMNGTIVNYSAMETRRLDLVIGVSYDANLAETKKVLTHILDNSQYVLKDPAYTVAVSELADSSVNFVVRPWVKGADYWPA
HFEILEQIKNALDEAGIGIPYPQMDLYVKETPAA
>Mature_274_residues
MDNLQGLMEQAPELIVTYGMKVLFAIIIFVIGKFLSKVAKKITTKILNKRKVDTTVVSFVANIAWALVFVFTIVATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRPCRVGDYVEAAGIAGTVDEITIFSTRLLTPDNKLVIAPNSA
MMNGTIVNYSAMETRRLDLVIGVSYDANLAETKKVLTHILDNSQYVLKDPAYTVAVSELADSSVNFVVRPWVKGADYWPA
HFEILEQIKNALDEAGIGIPYPQMDLYVKETPAA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=264, Percent_Identity=44.6969696969697, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1787591, Length=232, Percent_Identity=27.1551724137931, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786670, Length=259, Percent_Identity=26.2548262548263, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI2367355, Length=268, Percent_Identity=25.7462686567164, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 29572; Mature: 29572

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLQGLMEQAPELIVTYGMKVLFAIIIFVIGKFLSKVAKKITTKILNKRKVDTTVVSFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANIAWALVFVFTIVATLGQIGVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
CRVGDYVEAAGIAGTVDEITIFSTRLLTPDNKLVIAPNSAMMNGTIVNYSAMETRRLDLV
CCCCCHHHHCCCCCCHHHHHHHHEEEECCCCCEEECCCCCEECCEEEEECHHHHEEEEEE
IGVSYDANLAETKKVLTHILDNSQYVLKDPAYTVAVSELADSSVNFVVRPWVKGADYWPA
EEECCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCEEEECCCCCCCCCHH
HFEILEQIKNALDEAGIGIPYPQMDLYVKETPAA
HHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MDNLQGLMEQAPELIVTYGMKVLFAIIIFVIGKFLSKVAKKITTKILNKRKVDTTVVSFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANIAWALVFVFTIVATLGQIGVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
CRVGDYVEAAGIAGTVDEITIFSTRLLTPDNKLVIAPNSAMMNGTIVNYSAMETRRLDLV
CCCCCHHHHCCCCCCHHHHHHHHEEEECCCCCEEECCCCCEECCEEEEECHHHHEEEEEE
IGVSYDANLAETKKVLTHILDNSQYVLKDPAYTVAVSELADSSVNFVVRPWVKGADYWPA
EEECCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCEEEECCCCCCCCCHH
HFEILEQIKNALDEAGIGIPYPQMDLYVKETPAA
HHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]