Click here to switch to the map view.

The map label for this gene is trmB

Identifier: 167623089

GI number: 167623089

Start: 1406497

End: 1407210

Strand: Direct

Name: trmB

Synonym: Shal_1155

Alternate gene names: 167623089

Gene position: 1406497-1407210 (Clockwise)

Preceding gene: 167623086

Following gene: 167623090

Centisome position: 26.91

GC content: 45.24

Gene sequence:

>714_bases
ATGAGCGACGTAACAACCGCTGAATTCAATGAAGAAGGTAAATACCTTCGCAAAGTGAGAAGCTTTGTATTGAGAGAGGG
CCGTTTAACTAAAGGCCAAGCTCAAGCAATGGAGCAGCAATGGCCAATTATGGGCCTAGACTACACTCCTGAGTCGATTG
ATTTAGTTGAGGTATTTGGTCGTGAAGCGGATACCGTTTTAGAAATTGGTTTTGGTATGGGCGCGTCATTAGTTGCAATG
GCTAAAGCATCACCAGAGCTTAACTTTATTGGTATTGAAGTACATAAACCTGGTGTTGGCGCATGTTTGGCCGAAGCTGC
TGAAGCGGGCGTGACTAACTTACGTGTTTATCACCATGATGCGATTGAAGTGCTTGAGAATAGCATTGCTGAAGGCAGCT
TGGCTTGTGTGCAATTATTCTTCCCAGATCCTTGGCATAAAACTCGTCACCATAAGCGTCGCATCGTTCAAGCGCCATTT
GCTGAATTGATCCGCAGTAAACTAAAAGTGGGTGGTGTATTCCATCTGGCAACAGATTGGGAAAACTACAGCGAACATAT
GCTAGAAGTGATGAATGCCGCGCCTGGTTATAAAAACCAATCTGCGACAGGTGATGTTGTTGAACGTCCAGATCATCGTC
CATTGACTAAGTTCGAAGCGCGTGGCCACCGTTTAGGTCATGGTGTTTGGGATCTTATGTTTGAGCGCGTTTAA

Upstream 100 bases:

>100_bases
CCACAATAAGGCTTGCACTGAAGGTTGAACTTTGGATAATGCCTTTCTTTTTTAAATAATAGCTGTCTTATGCGAGAGCA
GGCTTAAAGCGAGGGCGAAA

Downstream 100 bases:

>100_bases
GCGTTAAACCGAATTTATACACTGTAAATAAGCTATCTAGGAGAACAACCATGGCAGCAAACCGTAGCCGTCGCTTACGT
AAGAAATTACGCGTTGATGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSDVTTAEFNEEGKYLRKVRSFVLREGRLTKGQAQAMEQQWPIMGLDYTPESIDLVEVFGREADTVLEIGFGMGASLVAM
AKASPELNFIGIEVHKPGVGACLAEAAEAGVTNLRVYHHDAIEVLENSIAEGSLACVQLFFPDPWHKTRHHKRRIVQAPF
AELIRSKLKVGGVFHLATDWENYSEHMLEVMNAAPGYKNQSATGDVVERPDHRPLTKFEARGHRLGHGVWDLMFERV

Sequences:

>Translated_237_residues
MSDVTTAEFNEEGKYLRKVRSFVLREGRLTKGQAQAMEQQWPIMGLDYTPESIDLVEVFGREADTVLEIGFGMGASLVAM
AKASPELNFIGIEVHKPGVGACLAEAAEAGVTNLRVYHHDAIEVLENSIAEGSLACVQLFFPDPWHKTRHHKRRIVQAPF
AELIRSKLKVGGVFHLATDWENYSEHMLEVMNAAPGYKNQSATGDVVERPDHRPLTKFEARGHRLGHGVWDLMFERV
>Mature_236_residues
SDVTTAEFNEEGKYLRKVRSFVLREGRLTKGQAQAMEQQWPIMGLDYTPESIDLVEVFGREADTVLEIGFGMGASLVAMA
KASPELNFIGIEVHKPGVGACLAEAAEAGVTNLRVYHHDAIEVLENSIAEGSLACVQLFFPDPWHKTRHHKRRIVQAPFA
ELIRSKLKVGGVFHLATDWENYSEHMLEVMNAAPGYKNQSATGDVVERPDHRPLTKFEARGHRLGHGVWDLMFERV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=236, Percent_Identity=66.5254237288136, Blast_Score=339, Evalue=9e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_SHEHH (B0TJD3)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673383.1
- ProteinModelPortal:   B0TJD3
- SMR:   B0TJD3
- GeneID:   5905707
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1155
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- BioCyc:   SHAL458817:SHAL_1155-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26524; Mature: 26392

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: BINDING 68-68 BINDING 93-93 BINDING 120-120 BINDING 143-143 BINDING 147-147 BINDING 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVTTAEFNEEGKYLRKVRSFVLREGRLTKGQAQAMEQQWPIMGLDYTPESIDLVEVFG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEECCCCCCCCHHHHHHHC
READTVLEIGFGMGASLVAMAKASPELNFIGIEVHKPGVGACLAEAAEAGVTNLRVYHHD
CCCHHEEEECCCCCHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEEEHH
AIEVLENSIAEGSLACVQLFFPDPWHKTRHHKRRIVQAPFAELIRSKLKVGGVFHLATDW
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEECCC
ENYSEHMLEVMNAAPGYKNQSATGDVVERPDHRPLTKFEARGHRLGHGVWDLMFERV
CHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SDVTTAEFNEEGKYLRKVRSFVLREGRLTKGQAQAMEQQWPIMGLDYTPESIDLVEVFG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEECCCCCCCCHHHHHHHC
READTVLEIGFGMGASLVAMAKASPELNFIGIEVHKPGVGACLAEAAEAGVTNLRVYHHD
CCCHHEEEECCCCCHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEEEHH
AIEVLENSIAEGSLACVQLFFPDPWHKTRHHKRRIVQAPFAELIRSKLKVGGVFHLATDW
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEECCC
ENYSEHMLEVMNAAPGYKNQSATGDVVERPDHRPLTKFEARGHRLGHGVWDLMFERV
CHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA