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The map label for this gene is puuB [H]

Identifier: 167622931

GI number: 167622931

Start: 1219728

End: 1221035

Strand: Reverse

Name: puuB [H]

Synonym: Shal_0992

Alternate gene names: 167622931

Gene position: 1221035-1219728 (Counterclockwise)

Preceding gene: 167622942

Following gene: 167622925

Centisome position: 23.36

GC content: 47.48

Gene sequence:

>1308_bases
ATGTTAACTAAGAGCCCAGTGCATAGCGACCAACACCCCGCATCTTATTACTTCGCAACAGCTAAAGAGCTGCATCAATC
ACCACGACTAGAAGAGGTGTTAGATGTCGATGTATGCGTAGTCGGCGGTGGATTTAGTGGTATCAATACGGCAATTGAAC
TGGCTCAAAAAGGCTTTAGTGTGGCACTTCTTGAGGCTAAGTGCATAGGTTGGGGAGCATCGGGCCGTAACGGCGGTGAG
CTCATTCGTGGCATTGGCCATGACGTTAGCCAGTTTCAAAATATCATTGGCAGCGAAGGCGTTAAAGCCATCGAACAGAT
GGGGTTTGAGGCGGTAGATATCGTCAGACAGCGCGTCGCTGAGCACAAAATCGATTGTAATTTACAGATGGGTTACTGCG
ACTTGGCTATACGTCCTCGTCATATGACGGAACTTGAAGAAGATTTTGAACACCTCAATAGTATCGGTTACGGCCATAAC
ATGACCTTACTTGATAAGTCTCGCATAGGTGAGGTCATCGGCTCAGATTTTTACCAAGGCGCACTGGTTGATATGGCCAG
TGGTCATCTGCACCCACTCAACTTAGCCCTTGGCGAGGCAAGAGTCGCCCGCAACTTAGGGGTTAAAGTGTATGAATATA
GCGCCGCACAAAAGATCGTTAAGGGCGATAAACCTAAGGTGATCACCGAACACGGTGAAGTGAATTGTCAGTATCTAGTC
TTGGCGGGTAATGCCTATTTGGGTCTGGATTTAGAAGAAAATATTGGCAGCAAGGTCCTTCCTGCGGGTACCTATGTGCT
GGCTACAGAGCCACTGACTCAAGAACAGTGTGACGCCATCATCCCACAAAATATGGCGTTTGCCGACTTAAGAGTCGACT
TGGATTATTACCATCTATCTGAAGACAATCGCCTGCTATTTGGTGGCTTATGTACCTACTCAGGTAAAGATCCTAAAGAT
ATCGAAGCAGCTCTCAGACCGAATATTGAGAAGGTATTTCCGCAGCTAAAAGGCGTGCGTATCGATTATGAATGGGGTGG
TATGATGGGTATCGGCGCCAATCGTCTACCACAAATTGGTCGTCTGCCCGATGCCAAGAATATCTTCTATGCCCAAGCCT
ATGCGGGCCATGGCGTGAATGCTACCCATATGGCGGCTAAACTAATCGCCGAGGCAATTACCACCCAAGCAGAACGTTTC
GATATCTTCGCCAAGGTGCCTCATATGACCTTCCCAGGTGGGCCACACCTACGTTCACCAATGTTAGCAATAGGCATGCT
TTATCATCGATTTAAGGATATTTTTTAA

Upstream 100 bases:

>100_bases
TTAAAAATAACGAACATCTGTAAATAATTTATTCACAACAGTTTGGGTATATTACTGCTACAAGTCCAATTTACAATAAA
AACATATATTTGGAGAAACG

Downstream 100 bases:

>100_bases
TACCCATTTTTAATCCCGTATCAATAATAAACGGACTTAATAGTCCGTTTTTTAATACCTGCAATCGGCATAAGTTTGTT
CGGTAATAATACCAATCAGT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MLTKSPVHSDQHPASYYFATAKELHQSPRLEEVLDVDVCVVGGGFSGINTAIELAQKGFSVALLEAKCIGWGASGRNGGE
LIRGIGHDVSQFQNIIGSEGVKAIEQMGFEAVDIVRQRVAEHKIDCNLQMGYCDLAIRPRHMTELEEDFEHLNSIGYGHN
MTLLDKSRIGEVIGSDFYQGALVDMASGHLHPLNLALGEARVARNLGVKVYEYSAAQKIVKGDKPKVITEHGEVNCQYLV
LAGNAYLGLDLEENIGSKVLPAGTYVLATEPLTQEQCDAIIPQNMAFADLRVDLDYYHLSEDNRLLFGGLCTYSGKDPKD
IEAALRPNIEKVFPQLKGVRIDYEWGGMMGIGANRLPQIGRLPDAKNIFYAQAYAGHGVNATHMAAKLIAEAITTQAERF
DIFAKVPHMTFPGGPHLRSPMLAIGMLYHRFKDIF

Sequences:

>Translated_435_residues
MLTKSPVHSDQHPASYYFATAKELHQSPRLEEVLDVDVCVVGGGFSGINTAIELAQKGFSVALLEAKCIGWGASGRNGGE
LIRGIGHDVSQFQNIIGSEGVKAIEQMGFEAVDIVRQRVAEHKIDCNLQMGYCDLAIRPRHMTELEEDFEHLNSIGYGHN
MTLLDKSRIGEVIGSDFYQGALVDMASGHLHPLNLALGEARVARNLGVKVYEYSAAQKIVKGDKPKVITEHGEVNCQYLV
LAGNAYLGLDLEENIGSKVLPAGTYVLATEPLTQEQCDAIIPQNMAFADLRVDLDYYHLSEDNRLLFGGLCTYSGKDPKD
IEAALRPNIEKVFPQLKGVRIDYEWGGMMGIGANRLPQIGRLPDAKNIFYAQAYAGHGVNATHMAAKLIAEAITTQAERF
DIFAKVPHMTFPGGPHLRSPMLAIGMLYHRFKDIF
>Mature_435_residues
MLTKSPVHSDQHPASYYFATAKELHQSPRLEEVLDVDVCVVGGGFSGINTAIELAQKGFSVALLEAKCIGWGASGRNGGE
LIRGIGHDVSQFQNIIGSEGVKAIEQMGFEAVDIVRQRVAEHKIDCNLQMGYCDLAIRPRHMTELEEDFEHLNSIGYGHN
MTLLDKSRIGEVIGSDFYQGALVDMASGHLHPLNLALGEARVARNLGVKVYEYSAAQKIVKGDKPKVITEHGEVNCQYLV
LAGNAYLGLDLEENIGSKVLPAGTYVLATEPLTQEQCDAIIPQNMAFADLRVDLDYYHLSEDNRLLFGGLCTYSGKDPKD
IEAALRPNIEKVFPQLKGVRIDYEWGGMMGIGANRLPQIGRLPDAKNIFYAQAYAGHGVNATHMAAKLIAEAITTQAERF
DIFAKVPHMTFPGGPHLRSPMLAIGMLYHRFKDIF

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=423, Percent_Identity=43.0260047281324, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47769; Mature: 47769

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKSPVHSDQHPASYYFATAKELHQSPRLEEVLDVDVCVVGGGFSGINTAIELAQKGFS
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHCCCE
VALLEAKCIGWGASGRNGGELIRGIGHDVSQFQNIIGSEGVKAIEQMGFEAVDIVRQRVA
EEEEEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
EHKIDCNLQMGYCDLAIRPRHMTELEEDFEHLNSIGYGHNMTLLDKSRIGEVIGSDFYQG
HCCCCCEEEECEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCHHHCC
ALVDMASGHLHPLNLALGEARVARNLGVKVYEYSAAQKIVKGDKPKVITEHGEVNCQYLV
HHHHHCCCCCEEEHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCEEECCCCCCEEEEE
LAGNAYLGLDLEENIGSKVLPAGTYVLATEPLTQEQCDAIIPQNMAFADLRVDLDYYHLS
EECCEEEECEEHHCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEEEEEEEEC
EDNRLLFGGLCTYSGKDPKDIEAALRPNIEKVFPQLKGVRIDYEWGGMMGIGANRLPQIG
CCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEECCCEEECCCCCCCCCC
RLPDAKNIFYAQAYAGHGVNATHMAAKLIAEAITTQAERFDIFAKVPHMTFPGGPHLRSP
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCH
MLAIGMLYHRFKDIF
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTKSPVHSDQHPASYYFATAKELHQSPRLEEVLDVDVCVVGGGFSGINTAIELAQKGFS
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHCCCE
VALLEAKCIGWGASGRNGGELIRGIGHDVSQFQNIIGSEGVKAIEQMGFEAVDIVRQRVA
EEEEEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
EHKIDCNLQMGYCDLAIRPRHMTELEEDFEHLNSIGYGHNMTLLDKSRIGEVIGSDFYQG
HCCCCCEEEECEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCHHHCC
ALVDMASGHLHPLNLALGEARVARNLGVKVYEYSAAQKIVKGDKPKVITEHGEVNCQYLV
HHHHHCCCCCEEEHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCEEECCCCCCEEEEE
LAGNAYLGLDLEENIGSKVLPAGTYVLATEPLTQEQCDAIIPQNMAFADLRVDLDYYHLS
EECCEEEECEEHHCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEEEEEEEEC
EDNRLLFGGLCTYSGKDPKDIEAALRPNIEKVFPQLKGVRIDYEWGGMMGIGANRLPQIG
CCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEECCCEEECCCCCCCCCC
RLPDAKNIFYAQAYAGHGVNATHMAAKLIAEAITTQAERFDIFAKVPHMTFPGGPHLRSP
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCH
MLAIGMLYHRFKDIF
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]