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The map label for this gene is hprA [H]

Identifier: 167622863

GI number: 167622863

Start: 1142831

End: 1143784

Strand: Direct

Name: hprA [H]

Synonym: Shal_0923

Alternate gene names: 167622863

Gene position: 1142831-1143784 (Clockwise)

Preceding gene: 167622862

Following gene: 167622865

Centisome position: 21.86

GC content: 47.27

Gene sequence:

>954_bases
ATGAAAGTAGTGGTACTGGATGGATATACGCTGAATCCTGGCGATCTTAGCTGGCAAGCTTTAGCACTGTTTGGCGAGTT
CACTTGCTATGATCGCAGTGCACCTAGTGAGGTGGTCGCACGAAGTTTGGATGCTGAAATTATTTTTACCAATAAGACCC
AGCTCGATGAAACGACACTAAAGCAGCTACCTAAGCTTAAATATATCGGTATCCTCGCAACTGGCACCAATGTCGTCGAT
CTAATCGCGGCCAAAGCGCTTGGCATTGTGGTTACTAATGTGCCGGGATATGGGCCCGATGCGGTTGCACAGATGGTATT
TGCGCATATCCTCCATGCCACGCAGCAGGTGGCTTTGCACTCAGATGCGGTAAAGCGTGGGCAGTGGAGTGAGTCTAAAG
ATTTCTGTTTTACTCTTATGCCACTGCAATCACTTAAAGGTAAGACGCTTGGATTAGTTGGCTTTGGTGATATCGGTAAA
GCAGTAGCTCAGATCGCGTTGGCTTTTGGTATGAAGGTCATCGTCAATACGCGAAACCCGCAAGTTAAACTACCTGAAAA
CTGTCATTGGGTTGAACAAGCTACGTTATTTAAACAGGCTGATATCGTTTCCTTGCACTGCCCACTCACCGCAGCGACAG
AAAATCTGGTTAACCCGCAAGTTCTTAGCATGATGAAACCTAGTGCAATATTAATTAATACAGCCCGAGGCGGACTCATC
GATGAAGCTGCATTGGCGGCGGCATTGGAGCAAGGGCTGTTAGCGTTTGCTGGCGTTGATGTATTGTCAACAGAGCCACC
AAGTGCTGATAATCCGCTGCTTAGCGCCAAGAATATAAGCATTTCGCCCCATAATGCGTGGGCGACGATAGAAGCGCGGC
AAAACTTATTGGATATCGCGATCGACAATTTAACGCGCTTTAGCCAAGGCCAAGTGAAAAACCGAGTTAATTGA

Upstream 100 bases:

>100_bases
GCCAATATTGGCGCCAGCTTTAAGTTCTAATTTGTCGTTTATAGAAGGACTTTTATAAAAGCCATTTAGTGTCGTTAATT
TATAACAAGGGCTGTTTTAG

Downstream 100 bases:

>100_bases
ACTCCTAAGTAACTTATTCGACATTTATTCGATTCAAGATAGGGTCTTATAACAAATAAGCCTCAAACTTAACGCTAAAT
CTGTTTAGCAAAAGTCTGAG

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKVVVLDGYTLNPGDLSWQALALFGEFTCYDRSAPSEVVARSLDAEIIFTNKTQLDETTLKQLPKLKYIGILATGTNVVD
LIAAKALGIVVTNVPGYGPDAVAQMVFAHILHATQQVALHSDAVKRGQWSESKDFCFTLMPLQSLKGKTLGLVGFGDIGK
AVAQIALAFGMKVIVNTRNPQVKLPENCHWVEQATLFKQADIVSLHCPLTAATENLVNPQVLSMMKPSAILINTARGGLI
DEAALAAALEQGLLAFAGVDVLSTEPPSADNPLLSAKNISISPHNAWATIEARQNLLDIAIDNLTRFSQGQVKNRVN

Sequences:

>Translated_317_residues
MKVVVLDGYTLNPGDLSWQALALFGEFTCYDRSAPSEVVARSLDAEIIFTNKTQLDETTLKQLPKLKYIGILATGTNVVD
LIAAKALGIVVTNVPGYGPDAVAQMVFAHILHATQQVALHSDAVKRGQWSESKDFCFTLMPLQSLKGKTLGLVGFGDIGK
AVAQIALAFGMKVIVNTRNPQVKLPENCHWVEQATLFKQADIVSLHCPLTAATENLVNPQVLSMMKPSAILINTARGGLI
DEAALAAALEQGLLAFAGVDVLSTEPPSADNPLLSAKNISISPHNAWATIEARQNLLDIAIDNLTRFSQGQVKNRVN
>Mature_317_residues
MKVVVLDGYTLNPGDLSWQALALFGEFTCYDRSAPSEVVARSLDAEIIFTNKTQLDETTLKQLPKLKYIGILATGTNVVD
LIAAKALGIVVTNVPGYGPDAVAQMVFAHILHATQQVALHSDAVKRGQWSESKDFCFTLMPLQSLKGKTLGLVGFGDIGK
AVAQIALAFGMKVIVNTRNPQVKLPENCHWVEQATLFKQADIVSLHCPLTAATENLVNPQVLSMMKPSAILINTARGGLI
DEAALAAALEQGLLAFAGVDVLSTEPPSADNPLLSAKNISISPHNAWATIEARQNLLDIAIDNLTRFSQGQVKNRVN

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=273, Percent_Identity=34.0659340659341, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4557499, Length=273, Percent_Identity=34.0659340659341, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI145580575, Length=263, Percent_Identity=33.8403041825095, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI23308577, Length=260, Percent_Identity=32.6923076923077, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI61743967, Length=279, Percent_Identity=31.8996415770609, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4557497, Length=279, Percent_Identity=31.8996415770609, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI6912396, Length=252, Percent_Identity=31.7460317460317, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI87082289, Length=280, Percent_Identity=35.7142857142857, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1787645, Length=306, Percent_Identity=31.6993464052288, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789279, Length=271, Percent_Identity=32.8413284132841, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1788660, Length=260, Percent_Identity=28.0769230769231, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=255, Percent_Identity=32.9411764705882, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25147481, Length=253, Percent_Identity=28.8537549407115, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=314, Percent_Identity=28.6624203821656, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=260, Percent_Identity=29.2307692307692, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=273, Percent_Identity=29.6703296703297, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324964, Length=313, Percent_Identity=28.7539936102236, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321253, Length=231, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325144, Length=150, Percent_Identity=30, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324980, Length=151, Percent_Identity=31.1258278145695, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24646446, Length=283, Percent_Identity=34.982332155477, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24646448, Length=283, Percent_Identity=34.982332155477, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24646452, Length=283, Percent_Identity=34.982332155477, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24646450, Length=283, Percent_Identity=34.982332155477, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI62472511, Length=283, Percent_Identity=34.982332155477, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28571528, Length=244, Percent_Identity=32.7868852459016, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574286, Length=252, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI45552429, Length=252, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585514, Length=252, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28574282, Length=252, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28574284, Length=252, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45551003, Length=252, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19921140, Length=273, Percent_Identity=30.03663003663, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585516, Length=249, Percent_Identity=26.9076305220884, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34036; Mature: 34036

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVVLDGYTLNPGDLSWQALALFGEFTCYDRSAPSEVVARSLDAEIIFTNKTQLDETTL
CEEEEEECEEECCCCCCHHHHHHHHHEEECCCCCCHHHHHHHCCCEEEEECCCCCCHHHH
KQLPKLKYIGILATGTNVVDLIAAKALGIVVTNVPGYGPDAVAQMVFAHILHATQQVALH
HHCCCEEEEEEEECCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
SDAVKRGQWSESKDFCFTLMPLQSLKGKTLGLVGFGDIGKAVAQIALAFGMKVIVNTRNP
HHHHHCCCCCCCCCCEEEEEEHHHCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCC
QVKLPENCHWVEQATLFKQADIVSLHCPLTAATENLVNPQVLSMMKPSAILINTARGGLI
CEECCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHCCCHHHHHHCCCCEEEEECCCCCCH
DEAALAAALEQGLLAFAGVDVLSTEPPSADNPLLSAKNISISPHNAWATIEARQNLLDIA
HHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEECCCEEECCCCCEEEHHHHHHHHHHH
IDNLTRFSQGQVKNRVN
HHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MKVVVLDGYTLNPGDLSWQALALFGEFTCYDRSAPSEVVARSLDAEIIFTNKTQLDETTL
CEEEEEECEEECCCCCCHHHHHHHHHEEECCCCCCHHHHHHHCCCEEEEECCCCCCHHHH
KQLPKLKYIGILATGTNVVDLIAAKALGIVVTNVPGYGPDAVAQMVFAHILHATQQVALH
HHCCCEEEEEEEECCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
SDAVKRGQWSESKDFCFTLMPLQSLKGKTLGLVGFGDIGKAVAQIALAFGMKVIVNTRNP
HHHHHCCCCCCCCCCEEEEEEHHHCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCC
QVKLPENCHWVEQATLFKQADIVSLHCPLTAATENLVNPQVLSMMKPSAILINTARGGLI
CEECCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHCCCHHHHHHCCCCEEEEECCCCCCH
DEAALAAALEQGLLAFAGVDVLSTEPPSADNPLLSAKNISISPHNAWATIEARQNLLDIA
HHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEECCCEEECCCCCEEEHHHHHHHHHHH
IDNLTRFSQGQVKNRVN
HHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]