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The map label for this gene is ldhA [H]

Identifier: 167622735

GI number: 167622735

Start: 990118

End: 991107

Strand: Direct

Name: ldhA [H]

Synonym: Shal_0795

Alternate gene names: 167622735

Gene position: 990118-991107 (Clockwise)

Preceding gene: 167622729

Following gene: 167622736

Centisome position: 18.94

GC content: 45.56

Gene sequence:

>990_bases
ATGAAAATTGGTTTTTTTAGCGCCAAACATTACGATATGCAGCATTTTAATCGAACCAACACGACCTTTGGTGCCAGTAT
CGAGTATTTTGATTACCGCCTCTGTATGCAAACTGTAAAACTCGCTGAAGGGTTTGAGGTCGTGTGCGCCTTTGTTAATG
ACTCTCTTTGTGAAGAAGTGCTAGTTGAACTCGCTCAAGGTGGCACTAAGATTATTGCTATGCGCTGCGCTGGCTTTAAT
AATGTCGATCTAGAAGCCGCCGAACGTTTAGGAATGAAGGTCGTTAATGTGCCTGCTTACTCTCCTGAGTCGGTTGCAGA
GCACACAGTAGCACTCATGCTTACTCTTAATCGCAAAATTCATAAAGCCTATCAGCGTACCCGTGATGCGAACTTCTCAC
TAGATGGGCTCGTGGGCTTTAATATGCATGGCCGCACGGTTGGGGTTATCGGCACGGGTAAGATAGGCCTCGCGACAATT
AAAGTATTGCTGGGTTTTGGCTGTAAAGTGGTGGCCCACGATCCTTACCCTAACCAAGACGTGATCGATTTAGGCGTTGA
GTACCTCACACTTGAGCAGCTGTATCCTATTTGTGATGTCATTAGCCTGCATTGCCCGTTGACCAAAGATAACTTTCATC
TATTGAGCCAGGGCAGCTTTGTAAAAATGAAGCCTGGCGTTATGGTCATCAATACCAGCCGTGGTGGTTTGCTTAATGCG
CTCGATGCAATGGAAGCTCTGAAGTCAGGACAACTGGGCTCACTAGGATTAGATGTATACGAGAATGAGAAAGAGCTTTT
CTTTGAAGATAAGTCGAGTGAAATCATTCAAGATGACGTATTTAGACGTTTGTCTGCATGTCACAACGTGATTTTTACAG
GTCACCAGGCATTTTTAACCGAAGAAGCATTAGGTGCGATTGCACACACGACACTGACAAATGTCACCCAGCTACTGGCG
GGAGAAGAGTGCCCCAACCAGCTGTATTAA

Upstream 100 bases:

>100_bases
TAGCCGCCAACAATGCTTAGATCAATAGCCCTCAAGACAAATCTGCTAATATCAAATACACTATTTTAGATTTCTTCCTC
ATTTAGATAAGGTTTCTACA

Downstream 100 bases:

>100_bases
AATAATTTGTTAGGTAATAAGTGGGCAGGAGCGACTTAGGTGCAAATAACTTGAGCGCTACTGCCTTTTTTATAGCGATA
AACCACTTGCTTAAGCTATT

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKIGFFSAKHYDMQHFNRTNTTFGASIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEVLVELAQGGTKIIAMRCAGFN
NVDLEAAERLGMKVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLDGLVGFNMHGRTVGVIGTGKIGLATI
KVLLGFGCKVVAHDPYPNQDVIDLGVEYLTLEQLYPICDVISLHCPLTKDNFHLLSQGSFVKMKPGVMVINTSRGGLLNA
LDAMEALKSGQLGSLGLDVYENEKELFFEDKSSEIIQDDVFRRLSACHNVIFTGHQAFLTEEALGAIAHTTLTNVTQLLA
GEECPNQLY

Sequences:

>Translated_329_residues
MKIGFFSAKHYDMQHFNRTNTTFGASIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEVLVELAQGGTKIIAMRCAGFN
NVDLEAAERLGMKVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLDGLVGFNMHGRTVGVIGTGKIGLATI
KVLLGFGCKVVAHDPYPNQDVIDLGVEYLTLEQLYPICDVISLHCPLTKDNFHLLSQGSFVKMKPGVMVINTSRGGLLNA
LDAMEALKSGQLGSLGLDVYENEKELFFEDKSSEIIQDDVFRRLSACHNVIFTGHQAFLTEEALGAIAHTTLTNVTQLLA
GEECPNQLY
>Mature_329_residues
MKIGFFSAKHYDMQHFNRTNTTFGASIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEVLVELAQGGTKIIAMRCAGFN
NVDLEAAERLGMKVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLDGLVGFNMHGRTVGVIGTGKIGLATI
KVLLGFGCKVVAHDPYPNQDVIDLGVEYLTLEQLYPICDVISLHCPLTKDNFHLLSQGSFVKMKPGVMVINTSRGGLLNA
LDAMEALKSGQLGSLGLDVYENEKELFFEDKSSEIIQDDVFRRLSACHNVIFTGHQAFLTEEALGAIAHTTLTNVTQLLA
GEECPNQLY

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=195, Percent_Identity=34.8717948717949, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI145580578, Length=263, Percent_Identity=30.7984790874525, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI4557499, Length=263, Percent_Identity=30.7984790874525, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI145580575, Length=263, Percent_Identity=30.7984790874525, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI61743967, Length=243, Percent_Identity=32.0987654320988, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4557497, Length=243, Percent_Identity=32.0987654320988, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI6912396, Length=212, Percent_Identity=28.3018867924528, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=60.3658536585366, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI87082289, Length=191, Percent_Identity=26.7015706806283, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789279, Length=228, Percent_Identity=28.5087719298246, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788660, Length=222, Percent_Identity=29.7297297297297, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=210, Percent_Identity=32.8571428571429, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=260, Percent_Identity=29.2307692307692, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324055, Length=199, Percent_Identity=30.1507537688442, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=238, Percent_Identity=29.8319327731092, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322116, Length=238, Percent_Identity=29.4117647058824, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=28.4313725490196, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=27.1428571428571, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646446, Length=275, Percent_Identity=31.6363636363636, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646448, Length=275, Percent_Identity=31.6363636363636, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646452, Length=275, Percent_Identity=31.6363636363636, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646450, Length=275, Percent_Identity=31.6363636363636, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI62472511, Length=275, Percent_Identity=31.6363636363636, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19921140, Length=260, Percent_Identity=27.3076923076923, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI28574286, Length=282, Percent_Identity=28.7234042553192, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45552429, Length=269, Percent_Identity=27.5092936802974, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24585514, Length=269, Percent_Identity=27.5092936802974, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28574282, Length=269, Percent_Identity=27.5092936802974, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28574284, Length=269, Percent_Identity=27.5092936802974, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45551003, Length=269, Percent_Identity=27.5092936802974, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI28571528, Length=292, Percent_Identity=23.6301369863014, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585516, Length=207, Percent_Identity=23.6714975845411, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36285; Mature: 36285

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGFFSAKHYDMQHFNRTNTTFGASIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEV
CEEECCCCCCCCHHHCCCCCCEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVELAQGGTKIIAMRCAGFNNVDLEAAERLGMKVVNVPAYSPESVAEHTVALMLTLNRKI
HHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
HKAYQRTRDANFSLDGLVGFNMHGRTVGVIGTGKIGLATIKVLLGFGCKVVAHDPYPNQD
HHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCC
VIDLGVEYLTLEQLYPICDVISLHCPLTKDNFHLLSQGSFVKMKPGVMVINTSRGGLLNA
HHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCCEEEEECCCCCHHHH
LDAMEALKSGQLGSLGLDVYENEKELFFEDKSSEIIQDDVFRRLSACHNVIFTGHQAFLT
HHHHHHHHCCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCHHHHH
EEALGAIAHTTLTNVTQLLAGEECPNQLY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKIGFFSAKHYDMQHFNRTNTTFGASIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEV
CEEECCCCCCCCHHHCCCCCCEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVELAQGGTKIIAMRCAGFNNVDLEAAERLGMKVVNVPAYSPESVAEHTVALMLTLNRKI
HHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
HKAYQRTRDANFSLDGLVGFNMHGRTVGVIGTGKIGLATIKVLLGFGCKVVAHDPYPNQD
HHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCC
VIDLGVEYLTLEQLYPICDVISLHCPLTKDNFHLLSQGSFVKMKPGVMVINTSRGGLLNA
HHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCCEEEEECCCCCHHHH
LDAMEALKSGQLGSLGLDVYENEKELFFEDKSSEIIQDDVFRRLSACHNVIFTGHQAFLT
HHHHHHHHCCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCHHHHH
EEALGAIAHTTLTNVTQLLAGEECPNQLY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]