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The map label for this gene is mrcB [H]

Identifier: 167622701

GI number: 167622701

Start: 942511

End: 944871

Strand: Direct

Name: mrcB [H]

Synonym: Shal_0761

Alternate gene names: 167622701

Gene position: 942511-944871 (Clockwise)

Preceding gene: 167622700

Following gene: 167622702

Centisome position: 18.03

GC content: 50.36

Gene sequence:

>2361_bases
ATGACAGATAAAGTTACACCTAAAACAGGCGCTAAAAGAGCGCCAGCGAAGAAGAGAATTGCCGCTAAAAAACCGCGAGC
TAAAAAGTCAGCTCCAAAGAAAGCGGGTGTGAGTTGGGGCAAGAAGCTCTGGTCGATCAGCTGGAAACTGGGCTTAGTTT
TAGCTGTGGCTATTGCTTCCTATGGGATCTACCTCGACCAAATTATTGCTCGAAAATTTGAGGGGCAAAAGTGGCATCTA
CCGGCACAGGTGTTTAGCCGCTCGATGGCACTTTATCCTGGTGCTGCGGTGAGCCATGGCCAGTTGATGTCAGAACTTAA
GTTGCTTGGCTATCGTAAGGTGGCTAACCCGCGTCAAGTCGGTGAATTTTCAGCATCTAAGACTAAAATCGATCTATGGC
GTCGACCATTTTTGCACCCTCAAGGGGATCAAGCCGAGCAGAGGGTGATGATCACCTTCGACAGTAACGGTGTGGTGTCG
GTCGCCCGTAGCAGCGATAAGCGCCAGTTAGCCGTGTTCCATCTTGAGCCGGTACTGCTGGACAGAATTATTACCGGTGA
CGGTGAAGATAGGCTGTTTGTGGCGACCGATAAGATCCCTAAGCCTATCGTTAATGCGCTTATCTTAGTTGAAGACCGCA
GTTTCTATGAGCATCACGGCGTTAATCCGTTTGCCATTATTCGTGCCGCTATGGTGAATATCAGCGCGGGACGTACTGTA
CAAGGTGGCTCGACCCTGACTCAGCAGCTGGCGAAGAACTTCTTCCTATCCAGTGAACGCTCGCTTAGCCGCAAGCTTCG
CGAAGCGCTGATGGCGATCATTATCGATTTTCGTTATGAGAAAGATGAGATCTTAGAAGCCTATCTTAATGAAGTGTATA
TGGGGCAAGATAAAGCCCGTGGCGTGCACGGTATGGGCTTAGCTTCGCAGTTTTATTTTGGTCGCCCAATTGCTGAGCTA
ACGGTGCCGCAGCAGGCATTTTTGGTGGCGGTCATTAAGGGGCCTTCTTATTACAACCCGTGGCGTTACCCTGAGCGTGC
ACAGAAGCGACGCGACTTAGTATTGCGTTTGCTGATGGAAGCGGGCGACCTGAATGTAACCCAGTATCAAGCGGCGGTAG
AGTCTCCTCTGGGGCTTCGATTAGCCAACAAGTCAGTGCATCAAAAGCTGCCTGCGTTCTTCTCTGTGGTTAAGCATGAA
CTTGCTAACCGTTATGGCGATGCTCTGTTGCAGGAATCTGGAATAAAGGTCTACACCACACTGGATCCTATGGCGCAAGA
GGCCGCTGAAAAAGCGGTTGAACAGACCCTTAAGCAGCTAGGGAAAAACGATAAAAGCCTGCAAGTGGGCATGGTACTGA
CCGATAAATATTCGGCGGGCATTGCCGCTATGGTGGGCGATAAGGTACCAAGCTATAAAGGCTTTAACCGCGCGGTAGAA
ATTCGCAGACCTATAGGCTCATTAATCAAGCCATTTGTCTATGCCACGGCCTTGAGTGATGGCAAGCAATACAATTTGAT
GTCGCCTCTAAAAGATCAACCTATTACGCTGAAAAATGGCCAAGGCAAGACTTGGTCACCACAGAACGTCGATAAAAAGT
TCCGTGATCAAGTGCCACTGTTAACGGCCTTTAAAAAATCGATGAACGTGCCGACGGTTAACCTTGGGATGGCGATTGGT
GTGAGTAGTGTGGCAACAACGCTAGATAAAGCAGGCTGGAAGGATAAGATCCCTGAATATCCCTCTATGCTACTTGGCGC
GGTGAATGGTTCGCCATTAATGGTCGCACAGGTATTCCAAACCATTGCCGATGATGGACGCTATCGCCACTTAAATTCGG
TGACGACAGTATTGAATGCCGAAAACCAACCGCTGGCAGCCTCTAGGCCGCAAGCGGTACAGGGTATTCCTGCTGCCAGT
AACTACTTGGTTAAATATGCCATGACCCAAGTTGTAGAGTCGGGTACGGCGAGACGTTTGGGCAGTGCATTCCCCTATGT
AACTTTAGCGGGTAAGACCGGTACCAGTAACGACTCGCGCGACTCTTGGTTTGCCGGTTTCGATGAGCGAAACGTGGCCG
CTATTTGGGTTGGCCGAGATGATAACGGCAAGACGGGGCTATACGGTAGTAGCGGCGCGATGGCGGTGTATCAGGCCTTC
TTAAATAACCGTGCGCCATTGAGCTTACGTATGGCGCCTGTCGATGGCGTGGTTCAAGGTTACTTTGAACGCAATACGGG
TAAGGCGGAAGAGCCACAATGCGCCAATGTGGTAAAGGTGCCTGCGCTGAGAGAAAGCTATCAGCCAGTGGCTAACTGCG
GTGAGCCACTGCCTTGGTGGAAGAAACTGATAGGTGGCTAA

Upstream 100 bases:

>100_bases
GCATTTATGGCCAGATGATCCCGCCAATACCCAACCGACCAAATTTACTAAAAAGAAGACGTTTAGCTCGCAATAAGCTT
GAGCACGTCGCGGACCGATA

Downstream 100 bases:

>100_bases
GCCCTTTATTGCCGATAATTTCGAGCCACCAAGGTGTTTTGAATCAACATACTTTCACTTCAGAGTATTAGAAGATCAAG
TGGCAGCGTTAGACTTTGAC

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 786; Mature: 785

Protein sequence:

>786_residues
MTDKVTPKTGAKRAPAKKRIAAKKPRAKKSAPKKAGVSWGKKLWSISWKLGLVLAVAIASYGIYLDQIIARKFEGQKWHL
PAQVFSRSMALYPGAAVSHGQLMSELKLLGYRKVANPRQVGEFSASKTKIDLWRRPFLHPQGDQAEQRVMITFDSNGVVS
VARSSDKRQLAVFHLEPVLLDRIITGDGEDRLFVATDKIPKPIVNALILVEDRSFYEHHGVNPFAIIRAAMVNISAGRTV
QGGSTLTQQLAKNFFLSSERSLSRKLREALMAIIIDFRYEKDEILEAYLNEVYMGQDKARGVHGMGLASQFYFGRPIAEL
TVPQQAFLVAVIKGPSYYNPWRYPERAQKRRDLVLRLLMEAGDLNVTQYQAAVESPLGLRLANKSVHQKLPAFFSVVKHE
LANRYGDALLQESGIKVYTTLDPMAQEAAEKAVEQTLKQLGKNDKSLQVGMVLTDKYSAGIAAMVGDKVPSYKGFNRAVE
IRRPIGSLIKPFVYATALSDGKQYNLMSPLKDQPITLKNGQGKTWSPQNVDKKFRDQVPLLTAFKKSMNVPTVNLGMAIG
VSSVATTLDKAGWKDKIPEYPSMLLGAVNGSPLMVAQVFQTIADDGRYRHLNSVTTVLNAENQPLAASRPQAVQGIPAAS
NYLVKYAMTQVVESGTARRLGSAFPYVTLAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTGLYGSSGAMAVYQAF
LNNRAPLSLRMAPVDGVVQGYFERNTGKAEEPQCANVVKVPALRESYQPVANCGEPLPWWKKLIGG

Sequences:

>Translated_786_residues
MTDKVTPKTGAKRAPAKKRIAAKKPRAKKSAPKKAGVSWGKKLWSISWKLGLVLAVAIASYGIYLDQIIARKFEGQKWHL
PAQVFSRSMALYPGAAVSHGQLMSELKLLGYRKVANPRQVGEFSASKTKIDLWRRPFLHPQGDQAEQRVMITFDSNGVVS
VARSSDKRQLAVFHLEPVLLDRIITGDGEDRLFVATDKIPKPIVNALILVEDRSFYEHHGVNPFAIIRAAMVNISAGRTV
QGGSTLTQQLAKNFFLSSERSLSRKLREALMAIIIDFRYEKDEILEAYLNEVYMGQDKARGVHGMGLASQFYFGRPIAEL
TVPQQAFLVAVIKGPSYYNPWRYPERAQKRRDLVLRLLMEAGDLNVTQYQAAVESPLGLRLANKSVHQKLPAFFSVVKHE
LANRYGDALLQESGIKVYTTLDPMAQEAAEKAVEQTLKQLGKNDKSLQVGMVLTDKYSAGIAAMVGDKVPSYKGFNRAVE
IRRPIGSLIKPFVYATALSDGKQYNLMSPLKDQPITLKNGQGKTWSPQNVDKKFRDQVPLLTAFKKSMNVPTVNLGMAIG
VSSVATTLDKAGWKDKIPEYPSMLLGAVNGSPLMVAQVFQTIADDGRYRHLNSVTTVLNAENQPLAASRPQAVQGIPAAS
NYLVKYAMTQVVESGTARRLGSAFPYVTLAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTGLYGSSGAMAVYQAF
LNNRAPLSLRMAPVDGVVQGYFERNTGKAEEPQCANVVKVPALRESYQPVANCGEPLPWWKKLIGG
>Mature_785_residues
TDKVTPKTGAKRAPAKKRIAAKKPRAKKSAPKKAGVSWGKKLWSISWKLGLVLAVAIASYGIYLDQIIARKFEGQKWHLP
AQVFSRSMALYPGAAVSHGQLMSELKLLGYRKVANPRQVGEFSASKTKIDLWRRPFLHPQGDQAEQRVMITFDSNGVVSV
ARSSDKRQLAVFHLEPVLLDRIITGDGEDRLFVATDKIPKPIVNALILVEDRSFYEHHGVNPFAIIRAAMVNISAGRTVQ
GGSTLTQQLAKNFFLSSERSLSRKLREALMAIIIDFRYEKDEILEAYLNEVYMGQDKARGVHGMGLASQFYFGRPIAELT
VPQQAFLVAVIKGPSYYNPWRYPERAQKRRDLVLRLLMEAGDLNVTQYQAAVESPLGLRLANKSVHQKLPAFFSVVKHEL
ANRYGDALLQESGIKVYTTLDPMAQEAAEKAVEQTLKQLGKNDKSLQVGMVLTDKYSAGIAAMVGDKVPSYKGFNRAVEI
RRPIGSLIKPFVYATALSDGKQYNLMSPLKDQPITLKNGQGKTWSPQNVDKKFRDQVPLLTAFKKSMNVPTVNLGMAIGV
SSVATTLDKAGWKDKIPEYPSMLLGAVNGSPLMVAQVFQTIADDGRYRHLNSVTTVLNAENQPLAASRPQAVQGIPAASN
YLVKYAMTQVVESGTARRLGSAFPYVTLAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTGLYGSSGAMAVYQAFL
NNRAPLSLRMAPVDGVVQGYFERNTGKAEEPQCANVVKVPALRESYQPVANCGEPLPWWKKLIGG

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=700, Percent_Identity=45, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI87082258, Length=264, Percent_Identity=38.6363636363636, Blast_Score=189, Evalue=8e-49,
Organism=Escherichia coli, GI1788867, Length=556, Percent_Identity=28.7769784172662, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1789601, Length=170, Percent_Identity=33.5294117647059, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 86611; Mature: 86480

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKVTPKTGAKRAPAKKRIAAKKPRAKKSAPKKAGVSWGKKLWSISWKLGLVLAVAIAS
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHH
YGIYLDQIIARKFEGQKWHLPAQVFSRSMALYPGAAVSHGQLMSELKLLGYRKVANPRQV
HHHHHHHHHHHHCCCCEEECCHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
GEFSASKTKIDLWRRPFLHPQGDQAEQRVMITFDSNGVVSVARSSDKRQLAVFHLEPVLL
CCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEEECCHHHH
DRIITGDGEDRLFVATDKIPKPIVNALILVEDRSFYEHHGVNPFAIIRAAMVNISAGRTV
HHHHCCCCCCEEEEECCCCCHHHHCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCCCEE
QGGSTLTQQLAKNFFLSSERSLSRKLREALMAIIIDFRYEKDEILEAYLNEVYMGQDKAR
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHC
GVHGMGLASQFYFGRPIAELTVPQQAFLVAVIKGPSYYNPWRYPERAQKRRDLVLRLLME
CCCCCCCHHHHHCCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
AGDLNVTQYQAAVESPLGLRLANKSVHQKLPAFFSVVKHELANRYGDALLQESGIKVYTT
CCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LDPMAQEAAEKAVEQTLKQLGKNDKSLQVGMVLTDKYSAGIAAMVGDKVPSYKGFNRAVE
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCHHHHH
IRRPIGSLIKPFVYATALSDGKQYNLMSPLKDQPITLKNGQGKTWSPQNVDKKFRDQVPL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCHH
LTAFKKSMNVPTVNLGMAIGVSSVATTLDKAGWKDKIPEYPSMLLGAVNGSPLMVAQVFQ
HHHHHHHCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCHHHHHHHH
TIADDGRYRHLNSVTTVLNAENQPLAASRPQAVQGIPAASNYLVKYAMTQVVESGTARRL
HHHCCCCEEHHHHHHHHHCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHH
GSAFPYVTLAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTGLYGSSGAMAVYQAF
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHH
LNNRAPLSLRMAPVDGVVQGYFERNTGKAEEPQCANVVKVPALRESYQPVANCGEPLPWW
HCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHCCHHHHCCCCCHHH
KKLIGG
HHHHCC
>Mature Secondary Structure 
TDKVTPKTGAKRAPAKKRIAAKKPRAKKSAPKKAGVSWGKKLWSISWKLGLVLAVAIAS
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHH
YGIYLDQIIARKFEGQKWHLPAQVFSRSMALYPGAAVSHGQLMSELKLLGYRKVANPRQV
HHHHHHHHHHHHCCCCEEECCHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
GEFSASKTKIDLWRRPFLHPQGDQAEQRVMITFDSNGVVSVARSSDKRQLAVFHLEPVLL
CCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEEECCHHHH
DRIITGDGEDRLFVATDKIPKPIVNALILVEDRSFYEHHGVNPFAIIRAAMVNISAGRTV
HHHHCCCCCCEEEEECCCCCHHHHCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCCCEE
QGGSTLTQQLAKNFFLSSERSLSRKLREALMAIIIDFRYEKDEILEAYLNEVYMGQDKAR
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHC
GVHGMGLASQFYFGRPIAELTVPQQAFLVAVIKGPSYYNPWRYPERAQKRRDLVLRLLME
CCCCCCCHHHHHCCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
AGDLNVTQYQAAVESPLGLRLANKSVHQKLPAFFSVVKHELANRYGDALLQESGIKVYTT
CCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LDPMAQEAAEKAVEQTLKQLGKNDKSLQVGMVLTDKYSAGIAAMVGDKVPSYKGFNRAVE
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCHHHHH
IRRPIGSLIKPFVYATALSDGKQYNLMSPLKDQPITLKNGQGKTWSPQNVDKKFRDQVPL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCHH
LTAFKKSMNVPTVNLGMAIGVSSVATTLDKAGWKDKIPEYPSMLLGAVNGSPLMVAQVFQ
HHHHHHHCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCHHHHHHHH
TIADDGRYRHLNSVTTVLNAENQPLAASRPQAVQGIPAASNYLVKYAMTQVVESGTARRL
HHHCCCCEEHHHHHHHHHCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHH
GSAFPYVTLAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTGLYGSSGAMAVYQAF
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHH
LNNRAPLSLRMAPVDGVVQGYFERNTGKAEEPQCANVVKVPALRESYQPVANCGEPLPWW
HCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHCCHHHHCCCCCHHH
KKLIGG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]