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The map label for this gene is pyrB

Identifier: 167622623

GI number: 167622623

Start: 841269

End: 842201

Strand: Reverse

Name: pyrB

Synonym: Shal_0683

Alternate gene names: 167622623

Gene position: 842201-841269 (Counterclockwise)

Preceding gene: 167622624

Following gene: 167622622

Centisome position: 16.11

GC content: 39.12

Gene sequence:

>933_bases
ATGACTAATACTATTTATAATAAAAATATAATATCGATCTCTGATCTGTCTCGTTCAGAACTTGAGCTTATTGTTGCCAC
AGCAAACGAATTGAAACAAAATCCACGTCCTGAATTATTGAAAAATAAGGTTGTCGCAAGTTGTTTTTTTGAGGCTTCGA
CGCGTACTAGGCTATCTTTTGAGACTGCAGTACAACGGCTAGGGGGAAGCATTATTGGCTTTCCGGATGGAGGTAACACT
TCATTAGGCAAGAAAGGTGAAACCTTGGCCGATTCGGTACAAGTAATTTCTTCCTACTGCGATGCTTTCTTTATTCGTCA
TAATCAAGAGGGTGCCGCTCGCCTTGCGAGTGAGTTTTCATCGGTACCCGTTATTAATGGAGGGGATGGTTCGAATCAGC
ATCCGACTCAGACCTTGCTCGACTTATTCAGCATTTATGAAACACAAGGTACCTTAGAGAAGTTACAAGTGGCTTTTGTC
GGTGATCTAAAATATGGTCGCACGGTACACTCTTTAACTCAGGCCTTATCCCTTTTCGATTGTGAATTTCATTTTGTTGC
TCCTAAAGCACTATTAATGCCGGACTATATTATCGATGAACTTAAAGAGAAAGGATGCAAATATACTCTGCATGAAACGT
TAGATGAAATTATGGATAGCCTAGATATCTTGTATATGACTCGTGTACAGAAGGAACGTTTTGACGAAACTGAATATCAA
CACTTAAAATCTAGCTTCATTCTAACTGCAAACATGCTTAAGGGTGTGAAAGATAATCTAAAAATCCTACATCCTTTGCC
TAGAGTCGATGAAATCACCACTGATGTTGATAGCACACCTTATGCATATTACTTCCAACAAGCAAAAAATGGTGTATATG
CCCGTCAAGCTCTACTCACCTTAGTTTTAACAAATGAATTTGGAGACCTGTGA

Upstream 100 bases:

>100_bases
TTTTGCATTGAGCTCTCAGTTTGTGACAAAACCGACACTCGGCTATACGAAGCGAGTAATATTCACCTCGTTCTACGCAT
CGAAATAGAAGACTTACTAT

Downstream 100 bases:

>100_bases
TGAAAAAACATCTCAAGGTAGAAGCTATTGAACAAGGTACTGTAATCGATCATATTTCATCCGGCCAAGGTATCAAAATA
TTGAAATTTTTTGAACTCAG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase 2; ATCase 2

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTNTIYNKNIISISDLSRSELELIVATANELKQNPRPELLKNKVVASCFFEASTRTRLSFETAVQRLGGSIIGFPDGGNT
SLGKKGETLADSVQVISSYCDAFFIRHNQEGAARLASEFSSVPVINGGDGSNQHPTQTLLDLFSIYETQGTLEKLQVAFV
GDLKYGRTVHSLTQALSLFDCEFHFVAPKALLMPDYIIDELKEKGCKYTLHETLDEIMDSLDILYMTRVQKERFDETEYQ
HLKSSFILTANMLKGVKDNLKILHPLPRVDEITTDVDSTPYAYYFQQAKNGVYARQALLTLVLTNEFGDL

Sequences:

>Translated_310_residues
MTNTIYNKNIISISDLSRSELELIVATANELKQNPRPELLKNKVVASCFFEASTRTRLSFETAVQRLGGSIIGFPDGGNT
SLGKKGETLADSVQVISSYCDAFFIRHNQEGAARLASEFSSVPVINGGDGSNQHPTQTLLDLFSIYETQGTLEKLQVAFV
GDLKYGRTVHSLTQALSLFDCEFHFVAPKALLMPDYIIDELKEKGCKYTLHETLDEIMDSLDILYMTRVQKERFDETEYQ
HLKSSFILTANMLKGVKDNLKILHPLPRVDEITTDVDSTPYAYYFQQAKNGVYARQALLTLVLTNEFGDL
>Mature_309_residues
TNTIYNKNIISISDLSRSELELIVATANELKQNPRPELLKNKVVASCFFEASTRTRLSFETAVQRLGGSIIGFPDGGNTS
LGKKGETLADSVQVISSYCDAFFIRHNQEGAARLASEFSSVPVINGGDGSNQHPTQTLLDLFSIYETQGTLEKLQVAFVG
DLKYGRTVHSLTQALSLFDCEFHFVAPKALLMPDYIIDELKEKGCKYTLHETLDEIMDSLDILYMTRVQKERFDETEYQH
LKSSFILTANMLKGVKDNLKILHPLPRVDEITTDVDSTPYAYYFQQAKNGVYARQALLTLVLTNEFGDL

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=42.8571428571429, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI38788445, Length=312, Percent_Identity=26.6025641025641, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=66.9902912621359, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1790703, Length=342, Percent_Identity=26.6081871345029, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786469, Length=335, Percent_Identity=25.6716417910448, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=322, Percent_Identity=41.304347826087, Blast_Score=228, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=41.6938110749186, Blast_Score=241, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6322373, Length=331, Percent_Identity=26.8882175226586, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=42.3948220064725, Blast_Score=253, Evalue=8e-68,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB2_SHEHH (B0TST3)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672917.1
- ProteinModelPortal:   B0TST3
- SMR:   B0TST3
- GeneID:   5906168
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0683
- HOGENOM:   HBG579429
- OMA:   AQYIDSH
- ProtClustDB:   PRK00856
- BioCyc:   SHAL458817:SHAL_0683-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34730; Mature: 34599

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTIYNKNIISISDLSRSELELIVATANELKQNPRPELLKNKVVASCFFEASTRTRLSF
CCCCCCCCCEEECCCCCCCHHHEEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHH
ETAVQRLGGSIIGFPDGGNTSLGKKGETLADSVQVISSYCDAFFIRHNQEGAARLASEFS
HHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SVPVINGGDGSNQHPTQTLLDLFSIYETQGTLEKLQVAFVGDLKYGRTVHSLTQALSLFD
CCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHC
CEFHFVAPKALLMPDYIIDELKEKGCKYTLHETLDEIMDSLDILYMTRVQKERFDETEYQ
CCEEEECCHHHHCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
HLKSSFILTANMLKGVKDNLKILHPLPRVDEITTDVDSTPYAYYFQQAKNGVYARQALLT
HHHHHHHHHHHHHHCHHHCHHHHCCCCCHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHH
LVLTNEFGDL
HHHHHCCCCC
>Mature Secondary Structure 
TNTIYNKNIISISDLSRSELELIVATANELKQNPRPELLKNKVVASCFFEASTRTRLSF
CCCCCCCCEEECCCCCCCHHHEEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHH
ETAVQRLGGSIIGFPDGGNTSLGKKGETLADSVQVISSYCDAFFIRHNQEGAARLASEFS
HHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SVPVINGGDGSNQHPTQTLLDLFSIYETQGTLEKLQVAFVGDLKYGRTVHSLTQALSLFD
CCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHC
CEFHFVAPKALLMPDYIIDELKEKGCKYTLHETLDEIMDSLDILYMTRVQKERFDETEYQ
CCEEEECCHHHHCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
HLKSSFILTANMLKGVKDNLKILHPLPRVDEITTDVDSTPYAYYFQQAKNGVYARQALLT
HHHHHHHHHHHHHHCHHHCHHHHCCCCCHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHH
LVLTNEFGDL
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA