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The map label for this gene is proP [H]

Identifier: 167622624

GI number: 167622624

Start: 842366

End: 843673

Strand: Reverse

Name: proP [H]

Synonym: Shal_0684

Alternate gene names: 167622624

Gene position: 843673-842366 (Counterclockwise)

Preceding gene: 167622625

Following gene: 167622623

Centisome position: 16.14

GC content: 34.56

Gene sequence:

>1308_bases
ATGATATCTCATATTAGTTCACTGACTATTGAGCCTAATGTATCAAATGATACTGAACAGACAACTTTAAAGCGAGCTTC
AACAGCTTGCTTTATTGGTAATTTCGTCGAATGGTTTGATTATGCATCATACGGCTTTTTAGCAACCATTATTGCTGTAT
CATTTTTTCCTAATTACGATACAACTAGCGCCCTAATGGCTACTTTTGCTATTTTTGCTATTTCATTTATTGTTCGCCCC
CTTGGAGGGATATTTTGGGGGCATATTGGTGACAAGATTGGTAGAAAAACAGCACTTTCAATGTCGATTATCATTATGTC
TTGCGCCACATTTTGTATTGCCTTACTACCTGACTATAACAGTATAGGTATAATGGCACCTCTCTTGCTGTTAGTTGCCA
GAATGTTTCAAGGATTTTCAGCTTCAGGCGAATATGCTGGTGCAGCAACATTTCTAACCGAAATCGCTCCTAAGGAAGAA
AAAGGTTTCTATGCTAGCATAGTCCCAGCCAGCGCTGCAGCAGGACTGTTATTTGGTTCGATATTTATATCTATCCTATA
TGCATTTCTATCATCAGCACAATTGCATGAATGGGGATGGCGCATCCCATTTTTATTAGCTGCGCCATTTGGATTAATTG
GTTTATACATTAGAGTTAAGATTGAAGATTCACCACAATTTGTAAAATTTAAAGAAGAAAATAAAGAAAAACAATCACCA
ATTCCATTACAAGTAATATTAGCACATCATAAGAAGCCATTACTATTAGGTTTTCTTGTGACTAGTTTGAATGCATTGGG
ATTTTATTTATTACTTAGTTATATGCCAACGTATATGACAGTACATTTAGAGGTAAAAGATACTACGGCTTTCGCAATAT
CATCGATAGTATTAGCTTTCTACATTTTATTTGTTTTTAGCATTGGTAAGTTGTCCGATAAATTTGGCAGGCGAAAATTA
CTTATGTTAGCTTCGCTTTGTTTCATATGTTTGTCTATCCCAATATTTATCATATTAGAATCTGCAAATATTTTTCAAAT
GACATTAGTATTAATTTTATTTAGCGCCTTTCTAGCATTAAATGATGGCTGTTTATCTTGCTATTTATGTGAGCTGTTCC
CAACTAACGTCAGATACACTGGATTTGCTCTATCATTTAATAGTGCTAATGCCCTATTTGGAGGTACTGCACCATTTATC
GCTACAACTTTAATTGCAGTAACAGGATTAAGTTACGCCCCTGGATTTTACTTAATGTTAATTGCATTGATGACATTTAT
TGCAATATTCAAATCGTATAAACAATGA

Upstream 100 bases:

>100_bases
GGAGGGGTAAGTGAAATGGTCGTGAGTTTGCTACAAAATACCAAGAAAAAAAGATTGGCAGCAAGTCTATAATAATCCAT
CACAAGAAGCTGGATAACTT

Downstream 100 bases:

>100_bases
CACTTAAGGAGTAGCCCTGTTCTACAGCAATCTAGCTGTACCTGAGAGGACTTATTCATTACTTTTTTGCATTGAGCTCT
CAGTTTGTGACAAAACCGAC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MISHISSLTIEPNVSNDTEQTTLKRASTACFIGNFVEWFDYASYGFLATIIAVSFFPNYDTTSALMATFAIFAISFIVRP
LGGIFWGHIGDKIGRKTALSMSIIIMSCATFCIALLPDYNSIGIMAPLLLLVARMFQGFSASGEYAGAATFLTEIAPKEE
KGFYASIVPASAAAGLLFGSIFISILYAFLSSAQLHEWGWRIPFLLAAPFGLIGLYIRVKIEDSPQFVKFKEENKEKQSP
IPLQVILAHHKKPLLLGFLVTSLNALGFYLLLSYMPTYMTVHLEVKDTTAFAISSIVLAFYILFVFSIGKLSDKFGRRKL
LMLASLCFICLSIPIFIILESANIFQMTLVLILFSAFLALNDGCLSCYLCELFPTNVRYTGFALSFNSANALFGGTAPFI
ATTLIAVTGLSYAPGFYLMLIALMTFIAIFKSYKQ

Sequences:

>Translated_435_residues
MISHISSLTIEPNVSNDTEQTTLKRASTACFIGNFVEWFDYASYGFLATIIAVSFFPNYDTTSALMATFAIFAISFIVRP
LGGIFWGHIGDKIGRKTALSMSIIIMSCATFCIALLPDYNSIGIMAPLLLLVARMFQGFSASGEYAGAATFLTEIAPKEE
KGFYASIVPASAAAGLLFGSIFISILYAFLSSAQLHEWGWRIPFLLAAPFGLIGLYIRVKIEDSPQFVKFKEENKEKQSP
IPLQVILAHHKKPLLLGFLVTSLNALGFYLLLSYMPTYMTVHLEVKDTTAFAISSIVLAFYILFVFSIGKLSDKFGRRKL
LMLASLCFICLSIPIFIILESANIFQMTLVLILFSAFLALNDGCLSCYLCELFPTNVRYTGFALSFNSANALFGGTAPFI
ATTLIAVTGLSYAPGFYLMLIALMTFIAIFKSYKQ
>Mature_435_residues
MISHISSLTIEPNVSNDTEQTTLKRASTACFIGNFVEWFDYASYGFLATIIAVSFFPNYDTTSALMATFAIFAISFIVRP
LGGIFWGHIGDKIGRKTALSMSIIIMSCATFCIALLPDYNSIGIMAPLLLLVARMFQGFSASGEYAGAATFLTEIAPKEE
KGFYASIVPASAAAGLLFGSIFISILYAFLSSAQLHEWGWRIPFLLAAPFGLIGLYIRVKIEDSPQFVKFKEENKEKQSP
IPLQVILAHHKKPLLLGFLVTSLNALGFYLLLSYMPTYMTVHLEVKDTTAFAISSIVLAFYILFVFSIGKLSDKFGRRKL
LMLASLCFICLSIPIFIILESANIFQMTLVLILFSAFLALNDGCLSCYLCELFPTNVRYTGFALSFNSANALFGGTAPFI
ATTLIAVTGLSYAPGFYLMLIALMTFIAIFKSYKQ

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=418, Percent_Identity=36.3636363636364, Blast_Score=284, Evalue=8e-78,
Organism=Escherichia coli, GI1788942, Length=428, Percent_Identity=34.8130841121495, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788292, Length=436, Percent_Identity=30.9633027522936, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=35.3488372093023, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI87082404, Length=396, Percent_Identity=26.5151515151515, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87082231, Length=238, Percent_Identity=23.9495798319328, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47857; Mature: 47857

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISHISSLTIEPNVSNDTEQTTLKRASTACFIGNFVEWFDYASYGFLATIIAVSFFPNYD
CCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TTSALMATFAIFAISFIVRPLGGIFWGHIGDKIGRKTALSMSIIIMSCATFCIALLPDYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SIGIMAPLLLLVARMFQGFSASGEYAGAATFLTEIAPKEEKGFYASIVPASAAAGLLFGS
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHH
IFISILYAFLSSAQLHEWGWRIPFLLAAPFGLIGLYIRVKIEDSPQFVKFKEENKEKQSP
HHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHEEEEEEEEECCCCCEEEECHHCCCCCCC
IPLQVILAHHKKPLLLGFLVTSLNALGFYLLLSYMPTYMTVHLEVKDTTAFAISSIVLAF
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHHHH
YILFVFSIGKLSDKFGRRKLLMLASLCFICLSIPIFIILESANIFQMTLVLILFSAFLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHH
NDGCLSCYLCELFPTNVRYTGFALSFNSANALFGGTAPFIATTLIAVTGLSYAPGFYLML
CCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
IALMTFIAIFKSYKQ
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MISHISSLTIEPNVSNDTEQTTLKRASTACFIGNFVEWFDYASYGFLATIIAVSFFPNYD
CCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TTSALMATFAIFAISFIVRPLGGIFWGHIGDKIGRKTALSMSIIIMSCATFCIALLPDYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SIGIMAPLLLLVARMFQGFSASGEYAGAATFLTEIAPKEEKGFYASIVPASAAAGLLFGS
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHH
IFISILYAFLSSAQLHEWGWRIPFLLAAPFGLIGLYIRVKIEDSPQFVKFKEENKEKQSP
HHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHEEEEEEEEECCCCCEEEECHHCCCCCCC
IPLQVILAHHKKPLLLGFLVTSLNALGFYLLLSYMPTYMTVHLEVKDTTAFAISSIVLAF
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHHHH
YILFVFSIGKLSDKFGRRKLLMLASLCFICLSIPIFIILESANIFQMTLVLILFSAFLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHH
NDGCLSCYLCELFPTNVRYTGFALSFNSANALFGGTAPFIATTLIAVTGLSYAPGFYLML
CCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
IALMTFIAIFKSYKQ
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]