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The map label for this gene is guaD [H]

Identifier: 167622599

GI number: 167622599

Start: 807095

End: 808411

Strand: Direct

Name: guaD [H]

Synonym: Shal_0659

Alternate gene names: 167622599

Gene position: 807095-808411 (Clockwise)

Preceding gene: 167622598

Following gene: 167622600

Centisome position: 15.44

GC content: 45.94

Gene sequence:

>1317_bases
ATGGAATATAGCCATACAATCAAAGCAATCCGGGCATCGATATTGGATATCGCTTGTACCGTTGATAGCCCAAAAGATAT
AGAAAAGAACCTACGATTTATTGAAGATGGTTTATTGCTCGTGGCCAACGGACATATTGAGTGGGTTGGCACGTGGGAGG
AGGGGCAATCTCGAATTCCAGCTAACGTTAGGGTGCGCACTTATCCAGGTAAAATAGTAATGCCTGGATTTATTGATACC
CATATTCACTATCCCCAAGCCGAGATGGTTGGCGCCTACGGCGAACAGTTGCTGGAGTGGTTGAATAACTATGTTTTTCC
CACCGAAGCTCGTTACAAAGATAAAGAATATGCCAGGGAGATGTCTGAGTTTTTTGTCAAACAGCTGCTGCGTAATGGCA
CCACCACAGCGCTAGTATTTGGTACCGTGCATCCTGAATCTGTTGATTCGCTCTTTGAAGTGGCTGAGAACATCAATATG
CGCCTGATTGCAGGCAAAGTGATGATGGATCGCAATGCACCTGACTACCTTGTCGACACCCCAGAGACTAGCTATTCAGA
GAGTAAGGCACTGATTGAGAAGTGGCATAAACGAGGACGCTTACTCTATGCCATTACCCCGCGTTTTGCTCCTACATCAA
CGCCTGAACAACTCGACATGGCCGGCAAGCTTAAGCAGGAGTTCCCTGATACTTATGTCCATACCCATCTGTGTGAGAAT
AAATCAGAGATTAAATGGGTCAAAGAGCTGTTTCCTGACCGTACTAGCTACTTAGATGTTTATCACCATCACGGCCTAAC
TGGCCCTAAGAGTATGTTTGCCCACAGTATTCACCTCGAAGACTGTGAGTGGGATTGCTTACAAAAAACCGATTCCGCCG
TGTCGTTTTGCCCTACCTCTAACCTCTATCTCGGTAGCGGTCTGTTTAATCTCAAAGAGGCATGGAAGCGCAATGTAAAG
GTTGGCGTAGGCACAGATATTGGTGCTGGAACCACCTTTAGCATAGTGCAGACCTTGAATGAGGCTTACAAGGTCATGCA
GCTGCAAGAGTATCGCCTATCGGCATTTGAAGCCTTCTATATGGCAACCTTAGGTGGAGCAAAATCCTTGTCGTTGGACC
ACGTCATTGGCAACTTTGATGTCGGTAAAGAAGCTGACTTTGTGGTGATAGATCCCTGCTCCACACCTTTGCAGCAATTA
AGATATGACAATTCAAAATGTTTAGCTGAGAAGCTGTTTGTGCTGATCACCTTAGGCGACGATCGCAGTATTTATCGTAC
CTATGTTGATGGTCGCCTGGTTTTTGAACGTACGTAA

Upstream 100 bases:

>100_bases
GCTCATAGCTCAATAAGTAAAAATGAAAAAGTAACACCTGTCGATAGTGCTCAACCACTAATCGAAGTGAACTAGATTAT
GAATAATATTAGAGGTCAAC

Downstream 100 bases:

>100_bases
ATTACATAATAATTTCAAAGGCATGCCTAGCTTTGCTAATTAGGCATATTCGGGAAATGTGGGCTAAAAATATAAATCAA
TATTCAAAATTGATGCGCTA

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MEYSHTIKAIRASILDIACTVDSPKDIEKNLRFIEDGLLLVANGHIEWVGTWEEGQSRIPANVRVRTYPGKIVMPGFIDT
HIHYPQAEMVGAYGEQLLEWLNNYVFPTEARYKDKEYAREMSEFFVKQLLRNGTTTALVFGTVHPESVDSLFEVAENINM
RLIAGKVMMDRNAPDYLVDTPETSYSESKALIEKWHKRGRLLYAITPRFAPTSTPEQLDMAGKLKQEFPDTYVHTHLCEN
KSEIKWVKELFPDRTSYLDVYHHHGLTGPKSMFAHSIHLEDCEWDCLQKTDSAVSFCPTSNLYLGSGLFNLKEAWKRNVK
VGVGTDIGAGTTFSIVQTLNEAYKVMQLQEYRLSAFEAFYMATLGGAKSLSLDHVIGNFDVGKEADFVVIDPCSTPLQQL
RYDNSKCLAEKLFVLITLGDDRSIYRTYVDGRLVFERT

Sequences:

>Translated_438_residues
MEYSHTIKAIRASILDIACTVDSPKDIEKNLRFIEDGLLLVANGHIEWVGTWEEGQSRIPANVRVRTYPGKIVMPGFIDT
HIHYPQAEMVGAYGEQLLEWLNNYVFPTEARYKDKEYAREMSEFFVKQLLRNGTTTALVFGTVHPESVDSLFEVAENINM
RLIAGKVMMDRNAPDYLVDTPETSYSESKALIEKWHKRGRLLYAITPRFAPTSTPEQLDMAGKLKQEFPDTYVHTHLCEN
KSEIKWVKELFPDRTSYLDVYHHHGLTGPKSMFAHSIHLEDCEWDCLQKTDSAVSFCPTSNLYLGSGLFNLKEAWKRNVK
VGVGTDIGAGTTFSIVQTLNEAYKVMQLQEYRLSAFEAFYMATLGGAKSLSLDHVIGNFDVGKEADFVVIDPCSTPLQQL
RYDNSKCLAEKLFVLITLGDDRSIYRTYVDGRLVFERT
>Mature_438_residues
MEYSHTIKAIRASILDIACTVDSPKDIEKNLRFIEDGLLLVANGHIEWVGTWEEGQSRIPANVRVRTYPGKIVMPGFIDT
HIHYPQAEMVGAYGEQLLEWLNNYVFPTEARYKDKEYAREMSEFFVKQLLRNGTTTALVFGTVHPESVDSLFEVAENINM
RLIAGKVMMDRNAPDYLVDTPETSYSESKALIEKWHKRGRLLYAITPRFAPTSTPEQLDMAGKLKQEFPDTYVHTHLCEN
KSEIKWVKELFPDRTSYLDVYHHHGLTGPKSMFAHSIHLEDCEWDCLQKTDSAVSFCPTSNLYLGSGLFNLKEAWKRNVK
VGVGTDIGAGTTFSIVQTLNEAYKVMQLQEYRLSAFEAFYMATLGGAKSLSLDHVIGNFDVGKEADFVVIDPCSTPLQQL
RYDNSKCLAEKLFVLITLGDDRSIYRTYVDGRLVFERT

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=389, Percent_Identity=33.6760925449871, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1789249, Length=438, Percent_Identity=72.1461187214612, Blast_Score=695, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6319963, Length=398, Percent_Identity=35.929648241206, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24643849, Length=431, Percent_Identity=36.1948955916473, Blast_Score=235, Evalue=6e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 49847; Mature: 49847

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSHTIKAIRASILDIACTVDSPKDIEKNLRFIEDGLLLVANGHIEWVGTWEEGQSRIP
CCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCEEEEECCCEEEEEEHHCCCCCCC
ANVRVRTYPGKIVMPGFIDTHIHYPQAEMVGAYGEQLLEWLNNYVFPTEARYKDKEYARE
CCEEEEECCCCEEECCEEECCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
MSEFFVKQLLRNGTTTALVFGTVHPESVDSLFEVAENINMRLIAGKVMMDRNAPDYLVDT
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCEECC
PETSYSESKALIEKWHKRGRLLYAITPRFAPTSTPEQLDMAGKLKQEFPDTYVHTHLCEN
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCC
KSEIKWVKELFPDRTSYLDVYHHHGLTGPKSMFAHSIHLEDCEWDCLQKTDSAVSFCPTS
CHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHEEEEECCCCCCHHHHCCCCCEEECCCC
NLYLGSGLFNLKEAWKRNVKVGVGTDIGAGTTFSIVQTLNEAYKVMQLQEYRLSAFEAFY
CEEECCCHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MATLGGAKSLSLDHVIGNFDVGKEADFVVIDPCSTPLQQLRYDNSKCLAEKLFVLITLGD
HHHCCCCCCCCHHHEECCCCCCCCCCEEEECCCCCHHHHHHCCCHHHHHCCEEEEEEECC
DRSIYRTYVDGRLVFERT
CCCEEEEEECCEEEEEEC
>Mature Secondary Structure
MEYSHTIKAIRASILDIACTVDSPKDIEKNLRFIEDGLLLVANGHIEWVGTWEEGQSRIP
CCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCEEEEECCCEEEEEEHHCCCCCCC
ANVRVRTYPGKIVMPGFIDTHIHYPQAEMVGAYGEQLLEWLNNYVFPTEARYKDKEYARE
CCEEEEECCCCEEECCEEECCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
MSEFFVKQLLRNGTTTALVFGTVHPESVDSLFEVAENINMRLIAGKVMMDRNAPDYLVDT
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCEECC
PETSYSESKALIEKWHKRGRLLYAITPRFAPTSTPEQLDMAGKLKQEFPDTYVHTHLCEN
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCC
KSEIKWVKELFPDRTSYLDVYHHHGLTGPKSMFAHSIHLEDCEWDCLQKTDSAVSFCPTS
CHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHEEEEECCCCCCHHHHCCCCCEEECCCC
NLYLGSGLFNLKEAWKRNVKVGVGTDIGAGTTFSIVQTLNEAYKVMQLQEYRLSAFEAFY
CEEECCCHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MATLGGAKSLSLDHVIGNFDVGKEADFVVIDPCSTPLQQLRYDNSKCLAEKLFVLITLGD
HHHCCCCCCCCHHHEECCCCCCCCCCEEEECCCCCHHHHHHCCCHHHHHCCEEEEEEECC
DRSIYRTYVDGRLVFERT
CCCEEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]