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The map label for this gene is metL [H]

Identifier: 167622563

GI number: 167622563

Start: 751123

End: 753516

Strand: Direct

Name: metL [H]

Synonym: Shal_0623

Alternate gene names: 167622563

Gene position: 751123-753516 (Clockwise)

Preceding gene: 167622562

Following gene: 167622564

Centisome position: 14.37

GC content: 48.16

Gene sequence:

>2394_bases
ATGGCACGCTGTCACTTACATAAATTTGGTGGCTCAAGTCTTGCGGATGCTGACTGTTACCGTCGCGTTGCTCATATTCT
GCTGACTCATGGTCACAGTGATGATTTAGTGGTGGTGTCTGCCGCGGGTAAAAGCACCAACTTCTTGTATAATTTACTTG
AGCTAAGTTCCTGTAAACAGTTGTGGCAAGAAGAGCTACAAATGTTGATTAGTTTCCAGCAAAATTTGATAGAGCAGTTG
CTCTCAAATGAACAGGCGCGCGCTCTAAGGGAGCGACTCTCTAACGATAAGGCGCAGCTGATCAGCTTGTTATCGCTAGA
GTCTATTAGTGATTACCAGAGCAACCAGGTGGTGAGCTTTGGTGAGCGCTGGTCTGCACGTTTATTGGCGGCGCTATTGC
GTGAGTCTGGTGTGGCGGCATCCGATGTTGATGCGCGCACACTCTTGATTGCCGATGAGGCTGCGGTACCACAAATTCGC
CTGCAGGAATCTCGTCAACGCGTTCATGCCATGACCGAAACGTACCCTAATGAGCGTTTAATTATTACCGGATTCATCTG
TGCTAATAACCGTGGCGAGACTTTGCTACTGGGACGCAATGGCTCCGATTTTAGCGCCACCTTGATTGCCAGTTTGGCCG
ATATCGAGCGGGTAACTATTTGGACCGATGTAGAGGGCGTGTTTAATGCTGATCCGAATAAGATTAATGATGCTAAGTTA
CTCAGTAGCTTGTCATTAGCCGAAGCAAACCGCTTGGCGCACCTAGGCTCTCCGGTGCTGCATAACAGAACTCTACAACC
CCTGTTTGATACTCAAGTAAGCCTAGCTGTACGTTCAAGTTATGCATCTCATACAGATTTTACCTTGATCGCACCTAAGA
GCTCATCGGCGAGTGCGCCAGTGGTCACTAATCTAAGCAGCGTCGCGCTATTTAACCTTGAGCTTAAGAGTGATTTTAAA
CAGCTTATTGAGCTTCTTGAAGAAACCGGCTTAAGTCCGCTGGCCTATTGGGAGCTAGGGCAAGACCGTGTAGAGCTCAC
TGTCACCCTAGAGAACCACAAGCAAGTATTGGCATTGCTGGACGTTAACCGTGATGCCTTAGGTGTGAGTGACATTAAGA
TCACCGAAGATCTTGGTTTAGTGGCGCTTGTGAGCGCAGATGCAAGCCTGTATCGCCGCGGCTTTGCCAGATTACTCAGC
CGTAATGCTAGACCGCTGTTTAATGATGGTATGAGCCTTGTGACCTTGGTGCCACAGGGACAAGTAAACTTGCTCACGCA
AAAAGTACACCGTCGCTGCGCCGGTCCTCGAAAACGTATTGGCGTACTCTTGTTAGGGGTGGGCAATATTGGTGAAGCTT
GGATTGAACTCTTTAGTGGTGCTAAGTCGGCACTGAATAAAGAGCTTGAAGCTAGCGTCGAGTTGGTCGGATTAGTCAGT
TCACGTAAGGCACTGATCAGCGACGAGGGCATCGAGTTGGGCTCGTGGAAGCAGGCCTTTGAAGAGCATGCGACACCTTG
GCAGTATAGTCACCTGTTTGATGAAGTGCATGAGCTTGAATGCGATGAAGTGATTGCACTCGATATCAGTGCCAGCGCCG
ATCTGACTCTTCAATACCCAGAGTTTTTTGCTCGTGGAATTCATGTTGTCAGTGCAAATAAGCTAGCGGGCTCAGGGCCA
CTAACTTTCTATCGAGAGCTGAAACAGCAGTTAGATAACCGCCGTCTATTTTGGCGTTATAACGCCAGTTGCGGTGCCGG
TCTACCGGTACAGCATGCATTGAATGATCTGCATAATAGTGGCGATAGCATTGAGGCTGTTGGCGGTATTTTTTCAGGTA
CCCTGTGTTGGTTATTTGAAAACTTCGATGCTAAGAAACCTTTCTCTGAGCTAGTCATAGAAGCTAGAGGCTTAGGGATC
ACCGAGCCCGATCCGCGTGATGATCTATCGGGTCGAGACATGCAGCGTAAGCTGCTTATTCTGGCTCGTGAAATCGGCTT
TGAAATTGAGCTTGAAGATATCGAGCTTAAGTCCTTGGTGCCGGATAATCTTGCTGAGTTGCCGTTAGAGCAATTTTTAT
TACGGATAAGTGAACTTGACGCCGACATGCTGCAACAGTTCGTCGCTGCGGCAGAGCAAGATAAAGTGCTGAGATATGTG
GCGTCTTTAGATAAGGTCGATGGTGAGCTAAAGGCTGAGGTAGGTATACAGTGGGTCGATATTTCTCATCCTTACGCTAA
CTTAACGCCAGGTGATAATGTGTTTGTTATCCGTAGTGAGTTTTATCAAGACAATCCATTGATAATTCGTGGACCTGGGG
CTGGCCGCTTAGTGACCGCCGCGGCTGTACAGTCAGACTTAGCGCATATTTGTCGAGATCTGCTGCAAGAATAA

Upstream 100 bases:

>100_bases
TATCCGTCTTTCTGTTGGTATCGAAGATGCTGATGATCTTGTTGCCGATATTGAAGCTGCGCTTAATGCCGCAGCCGCTT
GTTAATTAAAGGGGTATACG

Downstream 100 bases:

>100_bases
TTTTCATTTTAATCAATTGTAAAGGGACTCATTTGAGTCCCTTTTTTGTTTTCAGCCCTAAACCACATTCTTCAGAAAGT
AGATATTTTTACGGTCCCAA

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 797; Mature: 796

Protein sequence:

>797_residues
MARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYNLLELSSCKQLWQEELQMLISFQQNLIEQL
LSNEQARALRERLSNDKAQLISLLSLESISDYQSNQVVSFGERWSARLLAALLRESGVAASDVDARTLLIADEAAVPQIR
LQESRQRVHAMTETYPNERLIITGFICANNRGETLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
LSSLSLAEANRLAHLGSPVLHNRTLQPLFDTQVSLAVRSSYASHTDFTLIAPKSSSASAPVVTNLSSVALFNLELKSDFK
QLIELLEETGLSPLAYWELGQDRVELTVTLENHKQVLALLDVNRDALGVSDIKITEDLGLVALVSADASLYRRGFARLLS
RNARPLFNDGMSLVTLVPQGQVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWIELFSGAKSALNKELEASVELVGLVS
SRKALISDEGIELGSWKQAFEEHATPWQYSHLFDEVHELECDEVIALDISASADLTLQYPEFFARGIHVVSANKLAGSGP
LTFYRELKQQLDNRRLFWRYNASCGAGLPVQHALNDLHNSGDSIEAVGGIFSGTLCWLFENFDAKKPFSELVIEARGLGI
TEPDPRDDLSGRDMQRKLLILAREIGFEIELEDIELKSLVPDNLAELPLEQFLLRISELDADMLQQFVAAAEQDKVLRYV
ASLDKVDGELKAEVGIQWVDISHPYANLTPGDNVFVIRSEFYQDNPLIIRGPGAGRLVTAAAVQSDLAHICRDLLQE

Sequences:

>Translated_797_residues
MARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYNLLELSSCKQLWQEELQMLISFQQNLIEQL
LSNEQARALRERLSNDKAQLISLLSLESISDYQSNQVVSFGERWSARLLAALLRESGVAASDVDARTLLIADEAAVPQIR
LQESRQRVHAMTETYPNERLIITGFICANNRGETLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
LSSLSLAEANRLAHLGSPVLHNRTLQPLFDTQVSLAVRSSYASHTDFTLIAPKSSSASAPVVTNLSSVALFNLELKSDFK
QLIELLEETGLSPLAYWELGQDRVELTVTLENHKQVLALLDVNRDALGVSDIKITEDLGLVALVSADASLYRRGFARLLS
RNARPLFNDGMSLVTLVPQGQVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWIELFSGAKSALNKELEASVELVGLVS
SRKALISDEGIELGSWKQAFEEHATPWQYSHLFDEVHELECDEVIALDISASADLTLQYPEFFARGIHVVSANKLAGSGP
LTFYRELKQQLDNRRLFWRYNASCGAGLPVQHALNDLHNSGDSIEAVGGIFSGTLCWLFENFDAKKPFSELVIEARGLGI
TEPDPRDDLSGRDMQRKLLILAREIGFEIELEDIELKSLVPDNLAELPLEQFLLRISELDADMLQQFVAAAEQDKVLRYV
ASLDKVDGELKAEVGIQWVDISHPYANLTPGDNVFVIRSEFYQDNPLIIRGPGAGRLVTAAAVQSDLAHICRDLLQE
>Mature_796_residues
ARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYNLLELSSCKQLWQEELQMLISFQQNLIEQLL
SNEQARALRERLSNDKAQLISLLSLESISDYQSNQVVSFGERWSARLLAALLRESGVAASDVDARTLLIADEAAVPQIRL
QESRQRVHAMTETYPNERLIITGFICANNRGETLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKLL
SSLSLAEANRLAHLGSPVLHNRTLQPLFDTQVSLAVRSSYASHTDFTLIAPKSSSASAPVVTNLSSVALFNLELKSDFKQ
LIELLEETGLSPLAYWELGQDRVELTVTLENHKQVLALLDVNRDALGVSDIKITEDLGLVALVSADASLYRRGFARLLSR
NARPLFNDGMSLVTLVPQGQVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWIELFSGAKSALNKELEASVELVGLVSS
RKALISDEGIELGSWKQAFEEHATPWQYSHLFDEVHELECDEVIALDISASADLTLQYPEFFARGIHVVSANKLAGSGPL
TFYRELKQQLDNRRLFWRYNASCGAGLPVQHALNDLHNSGDSIEAVGGIFSGTLCWLFENFDAKKPFSELVIEARGLGIT
EPDPRDDLSGRDMQRKLLILAREIGFEIELEDIELKSLVPDNLAELPLEQFLLRISELDADMLQQFVAAAEQDKVLRYVA
SLDKVDGELKAEVGIQWVDISHPYANLTPGDNVFVIRSEFYQDNPLIIRGPGAGRLVTAAAVQSDLAHICRDLLQE

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0460

COG function: function code E; Homoserine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=806, Percent_Identity=42.1836228287841, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1786183, Length=818, Percent_Identity=29.9511002444988, Blast_Score=318, Evalue=7e-88,
Organism=Escherichia coli, GI1790455, Length=298, Percent_Identity=33.5570469798658, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322599, Length=347, Percent_Identity=34.5821325648415, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320893, Length=311, Percent_Identity=23.4726688102894, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 88070; Mature: 87939

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYNLLELSSCKQ
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
LWQEELQMLISFQQNLIEQLLSNEQARALRERLSNDKAQLISLLSLESISDYQSNQVVSF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEH
GERWSARLLAALLRESGVAASDVDARTLLIADEAAVPQIRLQESRQRVHAMTETYPNERL
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEE
IITGFICANNRGETLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
EEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHH
LSSLSLAEANRLAHLGSPVLHNRTLQPLFDTQVSLAVRSSYASHTDFTLIAPKSSSASAP
HHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
VVTNLSSVALFNLELKSDFKQLIELLEETGLSPLAYWELGQDRVELTVTLENHKQVLALL
CCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCEEEEEE
DVNRDALGVSDIKITEDLGLVALVSADASLYRRGFARLLSRNARPLFNDGMSLVTLVPQG
ECCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHCCCEEEEEECCC
QVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWIELFSGAKSALNKELEASVELVGLVS
CHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHH
SRKALISDEGIELGSWKQAFEEHATPWQYSHLFDEVHELECDEVIALDISASADLTLQYP
CCHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECH
EFFARGIHVVSANKLAGSGPLTFYRELKQQLDNRRLFWRYNASCGAGLPVQHALNDLHNS
HHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCC
GDSIEAVGGIFSGTLCWLFENFDAKKPFSELVIEARGLGITEPDPRDDLSGRDMQRKLLI
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LAREIGFEIELEDIELKSLVPDNLAELPLEQFLLRISELDADMLQQFVAAAEQDKVLRYV
HHHHCCCEEEEECEEHHHCCCCCHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ASLDKVDGELKAEVGIQWVDISHPYANLTPGDNVFVIRSEFYQDNPLIIRGPGAGRLVTA
HHHHHCCCCEEHCCCEEEEECCCCCCCCCCCCCEEEEEHHCCCCCCEEEECCCCCCCHHH
AAVQSDLAHICRDLLQE
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYNLLELSSCKQ
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
LWQEELQMLISFQQNLIEQLLSNEQARALRERLSNDKAQLISLLSLESISDYQSNQVVSF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEH
GERWSARLLAALLRESGVAASDVDARTLLIADEAAVPQIRLQESRQRVHAMTETYPNERL
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEE
IITGFICANNRGETLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
EEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHH
LSSLSLAEANRLAHLGSPVLHNRTLQPLFDTQVSLAVRSSYASHTDFTLIAPKSSSASAP
HHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
VVTNLSSVALFNLELKSDFKQLIELLEETGLSPLAYWELGQDRVELTVTLENHKQVLALL
CCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCEEEEEE
DVNRDALGVSDIKITEDLGLVALVSADASLYRRGFARLLSRNARPLFNDGMSLVTLVPQG
ECCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHCCCEEEEEECCC
QVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWIELFSGAKSALNKELEASVELVGLVS
CHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHH
SRKALISDEGIELGSWKQAFEEHATPWQYSHLFDEVHELECDEVIALDISASADLTLQYP
CCHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECH
EFFARGIHVVSANKLAGSGPLTFYRELKQQLDNRRLFWRYNASCGAGLPVQHALNDLHNS
HHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCC
GDSIEAVGGIFSGTLCWLFENFDAKKPFSELVIEARGLGITEPDPRDDLSGRDMQRKLLI
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LAREIGFEIELEDIELKSLVPDNLAELPLEQFLLRISELDADMLQQFVAAAEQDKVLRYV
HHHHCCCEEEEECEEHHHCCCCCHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ASLDKVDGELKAEVGIQWVDISHPYANLTPGDNVFVIRSEFYQDNPLIIRGPGAGRLVTA
HHHHHCCCCEEHCCCEEEEECCCCCCCCCCCCCEEEEEHHCCCCCCEEEECCCCCCCHHH
AAVQSDLAHICRDLLQE
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]