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The map label for this gene is metF [H]

Identifier: 167622564

GI number: 167622564

Start: 753742

End: 754632

Strand: Direct

Name: metF [H]

Synonym: Shal_0624

Alternate gene names: 167622564

Gene position: 753742-754632 (Clockwise)

Preceding gene: 167622563

Following gene: 167622565

Centisome position: 14.42

GC content: 47.47

Gene sequence:

>891_bases
ATGGGTTTTCATCACGCACAACATGCAACATCATTAAACCAGAGCTTATCTGAGCTAAATGGTGACATTAACGTTTCTTT
TGAATTTTTCCCGCCTTCAACTCCAGAGATGGAAAAGCTACTTTGGAACTCTATTCGTCGTTTAGAGCCATTAAATCCTA
AGTTTGTTTCTGTGACTTACGGTGCAAACTCAGGCGTTCGTGATAGAACTCACGGTGTGATCGAGCGTATCCAAAAAGAA
ACGGACTTAGTGGCTGCGCCGCACCTTACCTTGGTTGACGCCAGTGACGAAGAGTTAAAAGAACTGGCTAAGCATTACTG
GAACAGCGGTATTCGTGATATCGTCGCCCTGCGCGGAGACTTGCCAGAGGGTAGCCCTAAGCCTACGCGTTTTGCTAATG
ATCTTGTGAGTTTACTGCGCTCGGTGGCTGACTTTGATATTTCAGTGGCTGCGTATCCTGAAGTGCATCCCGATGCGAGT
AATGCCCAGTCTGACCTTATTCATCTAAAAAAGAAGATCGATGCTGGTGCTAATCGTGCGATAACTCAGTTCTTCTTCGA
TGTTGAGTCTTACCTACGTTTCCGTGACCGCTGCGCCGCGGCGGGGATCGACGCCGAGATCGTACCGGGTATCTTGCCTG
TCACTAACTTTAATCAGACTAAGCGTTTTGCAGCGATGACCAATGTGTCTATTCCTAGTTGGTTGCATCGTCAGTTTGAA
GGGCTAGAGGATGACCCTACGACACGCCAGATGGTTGGTGCGAATGTTGCCATCGATATGGTTAAAGTCTTGTCTCGTGA
AGGGGTGAAAGACTTCCATTTCTATACCCTAAACCGTGCTGAGCTAACTTATGCTATTTGCCACACCTTAGGGGTTAGGC
CGGGTAAGTAG

Upstream 100 bases:

>100_bases
TGCGCTTGACTTCAAACTCAGTTTCTCTAGTATATGGACATATAGACGTCCAAACGTCTATATTGGAATGCAAATTAATA
CTGAAGTTGAGGAAGTTGAA

Downstream 100 bases:

>100_bases
CTGACCTGGTTTCCCTCAAAAAAGCTAACTAATAATAACGATAGTTACATACTTTAAGTATGGGGATATCGAGAGTAGGA
ATTTCTGATAAGCTTGGTTA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MGFHHAQHATSLNQSLSELNGDINVSFEFFPPSTPEMEKLLWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQKE
TDLVAAPHLTLVDASDEELKELAKHYWNSGIRDIVALRGDLPEGSPKPTRFANDLVSLLRSVADFDISVAAYPEVHPDAS
NAQSDLIHLKKKIDAGANRAITQFFFDVESYLRFRDRCAAAGIDAEIVPGILPVTNFNQTKRFAAMTNVSIPSWLHRQFE
GLEDDPTTRQMVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPGK

Sequences:

>Translated_296_residues
MGFHHAQHATSLNQSLSELNGDINVSFEFFPPSTPEMEKLLWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQKE
TDLVAAPHLTLVDASDEELKELAKHYWNSGIRDIVALRGDLPEGSPKPTRFANDLVSLLRSVADFDISVAAYPEVHPDAS
NAQSDLIHLKKKIDAGANRAITQFFFDVESYLRFRDRCAAAGIDAEIVPGILPVTNFNQTKRFAAMTNVSIPSWLHRQFE
GLEDDPTTRQMVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPGK
>Mature_295_residues
GFHHAQHATSLNQSLSELNGDINVSFEFFPPSTPEMEKLLWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQKET
DLVAAPHLTLVDASDEELKELAKHYWNSGIRDIVALRGDLPEGSPKPTRFANDLVSLLRSVADFDISVAAYPEVHPDASN
AQSDLIHLKKKIDAGANRAITQFFFDVESYLRFRDRCAAAGIDAEIVPGILPVTNFNQTKRFAAMTNVSIPSWLHRQFEG
LEDDPTTRQMVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPGK

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=285, Percent_Identity=31.2280701754386, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI1790377, Length=295, Percent_Identity=71.5254237288136, Blast_Score=460, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=29.4964028776978, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=29.4964028776978, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=31.2925170068027, Blast_Score=142, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6321313, Length=290, Percent_Identity=28.9655172413793, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24662658, Length=260, Percent_Identity=26.5384615384615, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33070; Mature: 32939

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFHHAQHATSLNQSLSELNGDINVSFEFFPPSTPEMEKLLWNSIRRLEPLNPKFVSVTY
CCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
GANSGVRDRTHGVIERIQKETDLVAAPHLTLVDASDEELKELAKHYWNSGIRDIVALRGD
CCCCCCCHHHHHHHHHHHHHHHHEECCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCC
LPEGSPKPTRFANDLVSLLRSVADFDISVAAYPEVHPDASNAQSDLIHLKKKIDAGANRA
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHH
ITQFFFDVESYLRFRDRCAAAGIDAEIVPGILPVTNFNQTKRFAAMTNVSIPSWLHRQFE
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEECCCCCCHHHHHHHHHCCCCHHHHHHHHC
GLEDDPTTRQMVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPGK
CCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GFHHAQHATSLNQSLSELNGDINVSFEFFPPSTPEMEKLLWNSIRRLEPLNPKFVSVTY
CCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
GANSGVRDRTHGVIERIQKETDLVAAPHLTLVDASDEELKELAKHYWNSGIRDIVALRGD
CCCCCCCHHHHHHHHHHHHHHHHEECCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCC
LPEGSPKPTRFANDLVSLLRSVADFDISVAAYPEVHPDASNAQSDLIHLKKKIDAGANRA
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHH
ITQFFFDVESYLRFRDRCAAAGIDAEIVPGILPVTNFNQTKRFAAMTNVSIPSWLHRQFE
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEECCCCCCHHHHHHHHHCCCCHHHHHHHHC
GLEDDPTTRQMVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPGK
CCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]