Click here to switch to the map view.

The map label for this gene is dut

Identifier: 167622367

GI number: 167622367

Start: 502739

End: 503197

Strand: Reverse

Name: dut

Synonym: Shal_0427

Alternate gene names: 167622367

Gene position: 503197-502739 (Counterclockwise)

Preceding gene: 167622368

Following gene: 167622366

Centisome position: 9.63

GC content: 47.93

Gene sequence:

>459_bases
ATGAAAACACCAATCGAATTAAAGATTTTAGACTCACGCATCGGCACTGAATTTCCACTCCCAGCTTACGCAACACCAGG
CAGTGCAGGAATGGATCTTCGCGCCATCACCGATACCCAACTGGTTATACAGCCGGGTGAAACGGTACTTATCCCAACAG
GGATTGCCATTCACGTAGCAGACCCAAGCCTTGCGGCGATTATTTTACCTCGCTCAGGCCTTGGTCATAAGCACGGTATC
GTTCTGGGCAACTTGGTTGGTCTTATCGACTCAGATTATCAAGGGCCGTTAATGGTCTCTTGCTGGAACCGTGGCAGTGA
GCCTTTTACAATTGAAATTGGTGACAGACTCGCTCAGCTGGTATTTGTACCCGTTGTACAGGCAGAATTTAAACTTGTAG
ACGAGTTTAATCAGTCTGATCGCGGCACAGGTGGATTTGGTCACTCAGGAACCAAATAG

Upstream 100 bases:

>100_bases
GCGTATTTTGGAGTGATGGTAGCACCGATTTACCCGCTGTGAATAAAGACACTTTAGCCAAGCAATTGCTTACTCTAATA
GCGAACAAAATAAAAAACTG

Downstream 100 bases:

>100_bases
TCGATTTCAGCGAACTTAGCTTAAATCAATTTAAATGGATAGCATGGAGCAATGTTTCCTTGCCGTCTCGCTCAGTAGTA
ATCGAGTGAAGGACAGATAA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MKTPIELKILDSRIGTEFPLPAYATPGSAGMDLRAITDTQLVIQPGETVLIPTGIAIHVADPSLAAIILPRSGLGHKHGI
VLGNLVGLIDSDYQGPLMVSCWNRGSEPFTIEIGDRLAQLVFVPVVQAEFKLVDEFNQSDRGTGGFGHSGTK

Sequences:

>Translated_152_residues
MKTPIELKILDSRIGTEFPLPAYATPGSAGMDLRAITDTQLVIQPGETVLIPTGIAIHVADPSLAAIILPRSGLGHKHGI
VLGNLVGLIDSDYQGPLMVSCWNRGSEPFTIEIGDRLAQLVFVPVVQAEFKLVDEFNQSDRGTGGFGHSGTK
>Mature_152_residues
MKTPIELKILDSRIGTEFPLPAYATPGSAGMDLRAITDTQLVIQPGETVLIPTGIAIHVADPSLAAIILPRSGLGHKHGI
VLGNLVGLIDSDYQGPLMVSCWNRGSEPFTIEIGDRLAQLVFVPVVQAEFKLVDEFNQSDRGTGGFGHSGTK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=145, Percent_Identity=34.4827586206897, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI4503423, Length=145, Percent_Identity=34.4827586206897, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI70906441, Length=145, Percent_Identity=34.4827586206897, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1790071, Length=148, Percent_Identity=69.5945945945946, Blast_Score=225, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71988561, Length=154, Percent_Identity=33.1168831168831, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319729, Length=84, Percent_Identity=44.047619047619, Blast_Score=70, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24583610, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921126, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_SHEHH (B0TQK9)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672661.1
- ProteinModelPortal:   B0TQK9
- SMR:   B0TQK9
- GeneID:   5905350
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0427
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- BioCyc:   SHAL458817:SHAL_0427-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16174; Mature: 16174

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: BINDING 84-84 BINDING 98-98

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPIELKILDSRIGTEFPLPAYATPGSAGMDLRAITDTQLVIQPGETVLIPTGIAIHVA
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCEEEEECCEEEEEC
DPSLAAIILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRGSEPFTIEIGDRLAQL
CCCEEEEEEECCCCCCCCCEEEEHEEEEECCCCCCCEEEEEECCCCCCEEEEHHHHHHHH
VFVPVVQAEFKLVDEFNQSDRGTGGFGHSGTK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTPIELKILDSRIGTEFPLPAYATPGSAGMDLRAITDTQLVIQPGETVLIPTGIAIHVA
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCEEEEECCEEEEEC
DPSLAAIILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRGSEPFTIEIGDRLAQL
CCCEEEEEEECCCCCCCCCEEEEHEEEEECCCCCCCEEEEEECCCCCCEEEEHHHHHHHH
VFVPVVQAEFKLVDEFNQSDRGTGGFGHSGTK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA