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The map label for this gene is pleD [H]

Identifier: 167622059

GI number: 167622059

Start: 134992

End: 136449

Strand: Direct

Name: pleD [H]

Synonym: Shal_0118

Alternate gene names: 167622059

Gene position: 134992-136449 (Clockwise)

Preceding gene: 167622058

Following gene: 167622060

Centisome position: 2.58

GC content: 43.21

Gene sequence:

>1458_bases
ATGGGTGATAAGGGTTGGAACGATAAGAAACGTTTTATTTGGATTTTATCCTTATTGCTATTAGTTGCCTTCGTGGTGAC
GAGTGGAATTAGTTATCAGGTCGCCCATGATTCATTAACTGAGCAAATAAAAGAAAATAATTTACCCCTCACTAGCGATA
ATATTTACTCAGAAATTCAACAAGATCTGCTTAAACCTATTTTTATCTCTTCATTGATGGCACAGGATACTTTCGTTCGA
GATTGGACCATTAACCAAGAACAGAACCCTGAGAAACTTATTCGCTATCTGAAAGAGATCCAAGATAAGTATGGCACGGT
CACCAGCTTTTTTGTTTCAGAAGCTACGCGTAACTATTACCATTCGAGTGGCGTGCTCAAACGGATTAAAGATATTGATC
CGGAAGATGCCTGGTATTTTCGAGTACGCTCCCTACCCGAATATGAACAATATGAAGTGAATATCGATCCAGATACCGCA
GATCAGAATCGAACCGTTATCTACGTTAACTATAAGGTCTTTGATTTTGAGGGAAACTTTATAGGGGTTACCGGGGTTGG
CTTGGCGGTTGAAAGTGTAAAGCGTCTCATTGAACTCTATCAAACTCGTTATAACCGCAGCGTATATTTTACCGATAGGC
AAGGCAATATTACCTTGCATAGTCAACAGTATGATGGTGCTGCGACCCTACAAACTAGCCTCGGCCTTGAAAAGCTAGCA
ATTCGAATACTTGCCAGCCCAAGCACTTCATTCTCGTATCAAAAAGCGGGGAAAACTATCTTCCTTAATAGTCGTTTGGT
GCCGGAGTTTAAGTGGCACTTAATTGTGGAGCAGGATGAAGCGCGGGGTGAGCAAAAGCTGTTAACCACCTTTTGGGCTA
ATATCGGATTAAGTTTGTTAGTCACCATCGGGGTCCTGATCATCGCTAATATGACCTTGGGTAAATATCAGCGAAAGTTG
GAATTGATGGCATCAACCGACAAGCTAACAGGTGCGGCTAATCGACAAGCTTTCGAAGAACATTTTGCAAACTCATTAAC
GAGATCCGTGCTTGATAGCAGGCCGATGTCATTACTATTAATGGATATCGACCACTTTAAATTAGTCAATGACTCTTACG
GGCATAATATTGGTGACTTGGTTATCCAAACCGTTAGTCATATGCTAAAAAGCCAGCTTAAAGAGGATGACTTACTCTGT
CGTTGGGGCGGTGAGGAGTTTCTGATCTTGCTGCCTAACTGCAGCTTAAGCCAGAGCAGTGAGCTAGCCGAGAGGATAAG
AGAGCTGGTGGCAGGGCGACTATTGCGAGTCAATGGTTGTGAAATCAATGTCACCTTGAGCTGTGGTGCTGCGCAGCACC
GCGCATCTGAAACGGCCGAAGACCTGATCAATCGTGTTGATATTGCACTCTATCAAGCCAAGGAGCAGGGACGTAATCGA
GTGGTTCTTTCCTATTAG

Upstream 100 bases:

>100_bases
AGGCGATGCGAGCACCTTAGTTTTCAGTTATTATTCACAAAAGTTTAACAATATCGAGCAATTAAACTGCTAAGTTTGCT
GATGTTGAGTGAGGGCTATT

Downstream 100 bases:

>100_bases
CGGGTTAACTCGGATATAGAGTATCACTTTAATTTGCAACTCAATTAAGCTGCTACATCCATAGGTGATGGTGTTAGCAT
AGTTTACGTTTTTCTACAGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MGDKGWNDKKRFIWILSLLLLVAFVVTSGISYQVAHDSLTEQIKENNLPLTSDNIYSEIQQDLLKPIFISSLMAQDTFVR
DWTINQEQNPEKLIRYLKEIQDKYGTVTSFFVSEATRNYYHSSGVLKRIKDIDPEDAWYFRVRSLPEYEQYEVNIDPDTA
DQNRTVIYVNYKVFDFEGNFIGVTGVGLAVESVKRLIELYQTRYNRSVYFTDRQGNITLHSQQYDGAATLQTSLGLEKLA
IRILASPSTSFSYQKAGKTIFLNSRLVPEFKWHLIVEQDEARGEQKLLTTFWANIGLSLLVTIGVLIIANMTLGKYQRKL
ELMASTDKLTGAANRQAFEEHFANSLTRSVLDSRPMSLLLMDIDHFKLVNDSYGHNIGDLVIQTVSHMLKSQLKEDDLLC
RWGGEEFLILLPNCSLSQSSELAERIRELVAGRLLRVNGCEINVTLSCGAAQHRASETAEDLINRVDIALYQAKEQGRNR
VVLSY

Sequences:

>Translated_485_residues
MGDKGWNDKKRFIWILSLLLLVAFVVTSGISYQVAHDSLTEQIKENNLPLTSDNIYSEIQQDLLKPIFISSLMAQDTFVR
DWTINQEQNPEKLIRYLKEIQDKYGTVTSFFVSEATRNYYHSSGVLKRIKDIDPEDAWYFRVRSLPEYEQYEVNIDPDTA
DQNRTVIYVNYKVFDFEGNFIGVTGVGLAVESVKRLIELYQTRYNRSVYFTDRQGNITLHSQQYDGAATLQTSLGLEKLA
IRILASPSTSFSYQKAGKTIFLNSRLVPEFKWHLIVEQDEARGEQKLLTTFWANIGLSLLVTIGVLIIANMTLGKYQRKL
ELMASTDKLTGAANRQAFEEHFANSLTRSVLDSRPMSLLLMDIDHFKLVNDSYGHNIGDLVIQTVSHMLKSQLKEDDLLC
RWGGEEFLILLPNCSLSQSSELAERIRELVAGRLLRVNGCEINVTLSCGAAQHRASETAEDLINRVDIALYQAKEQGRNR
VVLSY
>Mature_484_residues
GDKGWNDKKRFIWILSLLLLVAFVVTSGISYQVAHDSLTEQIKENNLPLTSDNIYSEIQQDLLKPIFISSLMAQDTFVRD
WTINQEQNPEKLIRYLKEIQDKYGTVTSFFVSEATRNYYHSSGVLKRIKDIDPEDAWYFRVRSLPEYEQYEVNIDPDTAD
QNRTVIYVNYKVFDFEGNFIGVTGVGLAVESVKRLIELYQTRYNRSVYFTDRQGNITLHSQQYDGAATLQTSLGLEKLAI
RILASPSTSFSYQKAGKTIFLNSRLVPEFKWHLIVEQDEARGEQKLLTTFWANIGLSLLVTIGVLIIANMTLGKYQRKLE
LMASTDKLTGAANRQAFEEHFANSLTRSVLDSRPMSLLLMDIDHFKLVNDSYGHNIGDLVIQTVSHMLKSQLKEDDLLCR
WGGEEFLILLPNCSLSQSSELAERIRELVAGRLLRVNGCEINVTLSCGAAQHRASETAEDLINRVDIALYQAKEQGRNRV
VLSY

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=40.4761904761905, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1787262, Length=202, Percent_Identity=37.1287128712871, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=38.6075949367089, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI87082007, Length=204, Percent_Identity=35.2941176470588, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=34.9397590361446, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=31.1764705882353, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=35.8381502890173, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=35.8288770053476, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1787802, Length=196, Percent_Identity=28.0612244897959, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=30.5389221556886, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=31.4465408805031, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081974, Length=194, Percent_Identity=25.2577319587629, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081977, Length=154, Percent_Identity=32.4675324675325, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788956, Length=158, Percent_Identity=32.9113924050633, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55329; Mature: 55198

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDKGWNDKKRFIWILSLLLLVAFVVTSGISYQVAHDSLTEQIKENNLPLTSDNIYSEIQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHH
QDLLKPIFISSLMAQDTFVRDWTINQEQNPEKLIRYLKEIQDKYGTVTSFFVSEATRNYY
HHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
HSSGVLKRIKDIDPEDAWYFRVRSLPEYEQYEVNIDPDTADQNRTVIYVNYKVFDFEGNF
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCEEEEEEEEEEEECCCE
IGVTGVGLAVESVKRLIELYQTRYNRSVYFTDRQGNITLHSQQYDGAATLQTSLGLEKLA
EEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCHHHHHHCCHHHHH
IRILASPSTSFSYQKAGKTIFLNSRLVPEFKWHLIVEQDEARGEQKLLTTFWANIGLSLL
EEEEECCCCCCCHHHCCCEEEEECCCCCCEEEEEEEECHHCCCHHHHHHHHHHHHHHHHH
VTIGVLIIANMTLGKYQRKLELMASTDKLTGAANRQAFEEHFANSLTRSVLDSRPMSLLL
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
MDIDHFKLVNDSYGHNIGDLVIQTVSHMLKSQLKEDDLLCRWGGEEFLILLPNCSLSQSS
EECCHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCHH
ELAERIRELVAGRLLRVNGCEINVTLSCGAAQHRASETAEDLINRVDIALYQAKEQGRNR
HHHHHHHHHHCCCEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VVLSY
EEEEC
>Mature Secondary Structure 
GDKGWNDKKRFIWILSLLLLVAFVVTSGISYQVAHDSLTEQIKENNLPLTSDNIYSEIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHH
QDLLKPIFISSLMAQDTFVRDWTINQEQNPEKLIRYLKEIQDKYGTVTSFFVSEATRNYY
HHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
HSSGVLKRIKDIDPEDAWYFRVRSLPEYEQYEVNIDPDTADQNRTVIYVNYKVFDFEGNF
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCEEEEEEEEEEEECCCE
IGVTGVGLAVESVKRLIELYQTRYNRSVYFTDRQGNITLHSQQYDGAATLQTSLGLEKLA
EEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCHHHHHHCCHHHHH
IRILASPSTSFSYQKAGKTIFLNSRLVPEFKWHLIVEQDEARGEQKLLTTFWANIGLSLL
EEEEECCCCCCCHHHCCCEEEEECCCCCCEEEEEEEECHHCCCHHHHHHHHHHHHHHHHH
VTIGVLIIANMTLGKYQRKLELMASTDKLTGAANRQAFEEHFANSLTRSVLDSRPMSLLL
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
MDIDHFKLVNDSYGHNIGDLVIQTVSHMLKSQLKEDDLLCRWGGEEFLILLPNCSLSQSS
EECCHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCHH
ELAERIRELVAGRLLRVNGCEINVTLSCGAAQHRASETAEDLINRVDIALYQAKEQGRNR
HHHHHHHHHHCCCEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VVLSY
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA