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The map label for this gene is recF

Identifier: 167621944

GI number: 167621944

Start: 2806

End: 3903

Strand: Direct

Name: recF

Synonym: Shal_0003

Alternate gene names: 167621944

Gene position: 2806-3903 (Clockwise)

Preceding gene: 167621943

Following gene: 167621945

Centisome position: 0.05

GC content: 41.71

Gene sequence:

>1098_bases
ATGAGCCTGACGCGTCTTCATATCGAAACATTTCGAAATATCAGTTTGGCTCAGTTGGATCCCGGTGATGGTTTAAACCT
CATATATGGTCAAAATGGTAGTGGCAAGACGAGTATTCTTGAGGCTATTTATTTTTTGGGTATGGGGCGTTCATTTAGAA
GTCACCTGTCTCAACGAGTGATCCAACATAATGATGACAAATTAACCCTGTTTGCCAATTTAAGTGTCTGCGAGCAGGAA
AGCAAAATCGGTTTGCGTCGTTTTAGAAATGGCGAGACTGAGGTCAAGATTAATGGCGATAACATAAAACGTTTATCGAC
TTTAGCTGAAACATTACCTATCCAGGTGATTACTCCTGAGAGTTTTTCACTGCTGTTTGAAGGCCCAAAGTCGAGGCGTC
AGTTTATCGATTGGGGCGCATTTCATAGTGACAAAAGTTTTCATTTGGCATGGGCTAACGTAAGGCGAATTTTAAAGCAA
AGAAATCAGCTACTTAAAAACCAAGTTTCCTACAGTCAGATACAATATTGGGATAAGGAACTAGTACGCTATTCTGAGCA
GGTTACAGAGATACGTAAACAATATGTAGACTCGTTAAATGAACAACTTAAGGGTATAATCGGAGAGTTTTTACCTCTGG
TTGAAGTTAAGGTTTCTTTTACCCGAGGCTGGGATAGTAAAACAGATTTTGGGCAGCTACTCGAGACGCAATATCTTCGA
GATGTGGCGGCGGGTAATACCGGCAGTGGCCCCCATAAGGCTGATTTACGATTACGTGTTGGCGTCTTGCCTGTTCAAGA
TGCTTTATCCCGAGGACAATTGAAATTGTTAGTCTGTGCGCTGCGTATTGCACAGGGAAAGTTGTTAAAACAACAAACAG
ATAAAAATAGTATTTATCTGGTGGATGATCTTCCATCAGAATTAGATGCACAGCATAGAAAACTATTGTTGCAGCAATTA
ATGGATACCGGCGCGCAAGTTTTTGTCACCGCTATCGAGCCTGCAGCAATAGTCGATTCGTTAGCCACACCACCAAGTAA
GATGTTCCATGTGGAACAGGGGTGTGTAACGGTAATTGATAAACCGACGAGAGAATAA

Upstream 100 bases:

>100_bases
GTGGTCAATGGATATGCTAATCTATTTGGCTATTTTTCGTTTTAGCCTGCGTATTTATACTGTAGGTTGGAGCTACTTTT
GGGTGTGAATTGAGTACTAA

Downstream 100 bases:

>100_bases
TATGTCAGAGAATAGTTACGATTCTTCGAGCATCAAGGTTCTAAAGGGCCTTGATGCGGTACGTAAGAGACCAGGAATGT
ACATCGGTGACACCGATGAT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSLTRLHIETFRNISLAQLDPGDGLNLIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRVIQHNDDKLTLFANLSVCEQE
SKIGLRRFRNGETEVKINGDNIKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHSDKSFHLAWANVRRILKQ
RNQLLKNQVSYSQIQYWDKELVRYSEQVTEIRKQYVDSLNEQLKGIIGEFLPLVEVKVSFTRGWDSKTDFGQLLETQYLR
DVAAGNTGSGPHKADLRLRVGVLPVQDALSRGQLKLLVCALRIAQGKLLKQQTDKNSIYLVDDLPSELDAQHRKLLLQQL
MDTGAQVFVTAIEPAAIVDSLATPPSKMFHVEQGCVTVIDKPTRE

Sequences:

>Translated_365_residues
MSLTRLHIETFRNISLAQLDPGDGLNLIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRVIQHNDDKLTLFANLSVCEQE
SKIGLRRFRNGETEVKINGDNIKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHSDKSFHLAWANVRRILKQ
RNQLLKNQVSYSQIQYWDKELVRYSEQVTEIRKQYVDSLNEQLKGIIGEFLPLVEVKVSFTRGWDSKTDFGQLLETQYLR
DVAAGNTGSGPHKADLRLRVGVLPVQDALSRGQLKLLVCALRIAQGKLLKQQTDKNSIYLVDDLPSELDAQHRKLLLQQL
MDTGAQVFVTAIEPAAIVDSLATPPSKMFHVEQGCVTVIDKPTRE
>Mature_364_residues
SLTRLHIETFRNISLAQLDPGDGLNLIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRVIQHNDDKLTLFANLSVCEQES
KIGLRRFRNGETEVKINGDNIKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHSDKSFHLAWANVRRILKQR
NQLLKNQVSYSQIQYWDKELVRYSEQVTEIRKQYVDSLNEQLKGIIGEFLPLVEVKVSFTRGWDSKTDFGQLLETQYLRD
VAAGNTGSGPHKADLRLRVGVLPVQDALSRGQLKLLVCALRIAQGKLLKQQTDKNSIYLVDDLPSELDAQHRKLLLQQLM
DTGAQVFVTAIEPAAIVDSLATPPSKMFHVEQGCVTVIDKPTRE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=357, Percent_Identity=46.218487394958, Blast_Score=336, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_SHEHH (B0TLA6)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672238.1
- GeneID:   5906755
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0003
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK905538
- BioCyc:   SHAL458817:SHAL_0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41290; Mature: 41159

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTRLHIETFRNISLAQLDPGDGLNLIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRV
CCCEEEEHHHHCCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
IQHNDDKLTLFANLSVCEQESKIGLRRFRNGETEVKINGDNIKRLSTLAETLPIQVITPE
HHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCEEEECCC
SFSLLFEGPKSRRQFIDWGAFHSDKSFHLAWANVRRILKQRNQLLKNQVSYSQIQYWDKE
CCEEEECCCHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRYSEQVTEIRKQYVDSLNEQLKGIIGEFLPLVEVKVSFTRGWDSKTDFGQLLETQYLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHH
DVAAGNTGSGPHKADLRLRVGVLPVQDALSRGQLKLLVCALRIAQGKLLKQQTDKNSIYL
HHHCCCCCCCCCCCCEEEEEECEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
VDDLPSELDAQHRKLLLQQLMDTGAQVFVTAIEPAAIVDSLATPPSKMFHVEQGCVTVID
EECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCHHHHEEECCCCEEECC
KPTRE
CCCCC
>Mature Secondary Structure 
SLTRLHIETFRNISLAQLDPGDGLNLIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRV
CCEEEEHHHHCCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
IQHNDDKLTLFANLSVCEQESKIGLRRFRNGETEVKINGDNIKRLSTLAETLPIQVITPE
HHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCEEEECCC
SFSLLFEGPKSRRQFIDWGAFHSDKSFHLAWANVRRILKQRNQLLKNQVSYSQIQYWDKE
CCEEEECCCHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRYSEQVTEIRKQYVDSLNEQLKGIIGEFLPLVEVKVSFTRGWDSKTDFGQLLETQYLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHH
DVAAGNTGSGPHKADLRLRVGVLPVQDALSRGQLKLLVCALRIAQGKLLKQQTDKNSIYL
HHHCCCCCCCCCCCCEEEEEECEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
VDDLPSELDAQHRKLLLQQLMDTGAQVFVTAIEPAAIVDSLATPPSKMFHVEQGCVTVID
EECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCHHHHEEECCCCEEECC
KPTRE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA