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The map label for this gene is dnaN [H]

Identifier: 167621943

GI number: 167621943

Start: 1486

End: 2586

Strand: Direct

Name: dnaN [H]

Synonym: Shal_0002

Alternate gene names: 167621943

Gene position: 1486-2586 (Clockwise)

Preceding gene: 167621942

Following gene: 167621944

Centisome position: 0.03

GC content: 44.87

Gene sequence:

>1101_bases
ATGAAATTTTCAATTGATAGGGACGCCTTATTAAAACCGCTTCAACTAGTTAGTGGCGCGGTTGAAAGACGTCATAACTT
GCCCATCTTGGCCAATTTACTCGTTGAAGTAAGTGGTCACTCACTTAAGATGACTGGTACCGATTTAGAAGTTGAGTTAG
TCGGTGAGGTTCAGCTCGAAGGTGAGGTTGTTGAAGGTCGTACTACGGTCCCAGCGAAGAAGTTTTTGGATATCGTTAAG
TCTCTACCTGATCAGGTTGAGCTTAAGATAGAGCAGCAAGAAAATCGTTTACTGTTACGTTCAGGCCGCAGTCGCTTTAC
CTTGGCTACATTGCCAGCTGAGGAGTATCCAAATGTCGATGCCTTTGAGGCTGAGATTGAGTTTACTCTGAAGCAGGGTA
CGATGAAGTCGTTGATCGACGCGACGCAATTCTCTATGGCTAACCAAGACGTACGTTATTACCTCAATGGTTTACTGCTT
GAGACCGAAGGTAACGTATTGCGTGCCATTGCCACCGATGGGCATCGCTTGGCGCTAAGCCACCGTATGATCGAAGCCTC
TCTACCAGAGAAGCAGGTGATTGTACCGCGTAAAGGCGTATTAGAGCTGATGCGCTCGTTTGAAGCTGACGATTTAGACG
TCACTATTGCGATTGGCGATAATGCTATTAGAGCGACAACGGCGACGGCGGTATTTACCAGTAAGTTGGTTGATGGTCGC
TTCCCAGATTATCGTCGTGTATTACCAAAAGGTGGCGACAAAATTGTTATAGCAAGCCGTCTGCAGCTAAAACAGGCATT
ATTGCGTGCTTCTATCCTGTCGAATGAGAAGTTCCGTGGTGTGCGGGTACAGCTGGAAAACAACCTTATTAAGATCACCG
CTAATAACCCTGAACAGGAAGAGGCTGAAGAGATCTTAGATGTTGAATATGAGAATACACCGCTAGAGATTGGTTTTAAC
GTCAGTTATCTGCTTGATGTATTAAATAACCTGCCTTCAGACGATGTACGTATTACTTTGATAGATGGCAACTCAAGTGC
GCTTATCGATAATCACATAGAGCAAGATTCTATGTATGTTGTAATGCCAATGAGGTTGTAG

Upstream 100 bases:

>100_bases
CGTAAAATTGCGCAGTTGCGTGAAGAAAGTCATGACATTAAAGAAGATTATGCTAACTTGATAAGAACCTTATCTTCTTA
ATATCTGGGATATTGAACCA

Downstream 100 bases:

>100_bases
ATATTTTCTTTAGCAAATATCTTATTCTTACCAATTAAAAGACTGATTAATCAGTCTTTTTTTGTTTCTAATGTTTAGCG
TTTTTCTTGTCTTATTTTGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGEVQLEGEVVEGRTTVPAKKFLDIVK
SLPDQVELKIEQQENRLLLRSGRSRFTLATLPAEEYPNVDAFEAEIEFTLKQGTMKSLIDATQFSMANQDVRYYLNGLLL
ETEGNVLRAIATDGHRLALSHRMIEASLPEKQVIVPRKGVLELMRSFEADDLDVTIAIGDNAIRATTATAVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRLQLKQALLRASILSNEKFRGVRVQLENNLIKITANNPEQEEAEEILDVEYENTPLEIGFN
VSYLLDVLNNLPSDDVRITLIDGNSSALIDNHIEQDSMYVVMPMRL

Sequences:

>Translated_366_residues
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGEVQLEGEVVEGRTTVPAKKFLDIVK
SLPDQVELKIEQQENRLLLRSGRSRFTLATLPAEEYPNVDAFEAEIEFTLKQGTMKSLIDATQFSMANQDVRYYLNGLLL
ETEGNVLRAIATDGHRLALSHRMIEASLPEKQVIVPRKGVLELMRSFEADDLDVTIAIGDNAIRATTATAVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRLQLKQALLRASILSNEKFRGVRVQLENNLIKITANNPEQEEAEEILDVEYENTPLEIGFN
VSYLLDVLNNLPSDDVRITLIDGNSSALIDNHIEQDSMYVVMPMRL
>Mature_366_residues
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGEVQLEGEVVEGRTTVPAKKFLDIVK
SLPDQVELKIEQQENRLLLRSGRSRFTLATLPAEEYPNVDAFEAEIEFTLKQGTMKSLIDATQFSMANQDVRYYLNGLLL
ETEGNVLRAIATDGHRLALSHRMIEASLPEKQVIVPRKGVLELMRSFEADDLDVTIAIGDNAIRATTATAVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRLQLKQALLRASILSNEKFRGVRVQLENNLIKITANNPEQEEAEEILDVEYENTPLEIGFN
VSYLLDVLNNLPSDDVRITLIDGNSSALIDNHIEQDSMYVVMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=59.2896174863388, Blast_Score=434, Evalue=1e-123,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40932; Mature: 40932

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGEVQLE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHCCCCEEEEECCCEEEEEEEEEEEE
GEVVEGRTTVPAKKFLDIVKSLPDQVELKIEQQENRLLLRSGRSRFTLATLPAEEYPNVD
CEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEEEEECCHHHCCCCC
AFEAEIEFTLKQGTMKSLIDATQFSMANQDVRYYLNGLLLETEGNVLRAIATDGHRLALS
EEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHEEECEEEEEECCCEEEEEECCCCEEEEH
HRMIEASLPEKQVIVPRKGVLELMRSFEADDLDVTIAIGDNAIRATTATAVFTSKLVDGR
HHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHCCC
FPDYRRVLPKGGDKIVIASRLQLKQALLRASILSNEKFRGVRVQLENNLIKITANNPEQE
CCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCCCHH
EAEEILDVEYENTPLEIGFNVSYLLDVLNNLPSDDVRITLIDGNSSALIDNHIEQDSMYV
HHHHHHCCEECCCCEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCCCEEE
VMPMRL
EEEECC
>Mature Secondary Structure
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGEVQLE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHCCCCEEEEECCCEEEEEEEEEEEE
GEVVEGRTTVPAKKFLDIVKSLPDQVELKIEQQENRLLLRSGRSRFTLATLPAEEYPNVD
CEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEEEEECCHHHCCCCC
AFEAEIEFTLKQGTMKSLIDATQFSMANQDVRYYLNGLLLETEGNVLRAIATDGHRLALS
EEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHEEECEEEEEECCCEEEEEECCCCEEEEH
HRMIEASLPEKQVIVPRKGVLELMRSFEADDLDVTIAIGDNAIRATTATAVFTSKLVDGR
HHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHCCC
FPDYRRVLPKGGDKIVIASRLQLKQALLRASILSNEKFRGVRVQLENNLIKITANNPEQE
CCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCCCHH
EAEEILDVEYENTPLEIGFNVSYLLDVLNNLPSDDVRITLIDGNSSALIDNHIEQDSMYV
HHHHHHCCEECCCCEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCCCEEE
VMPMRL
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]