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The map label for this gene is ytzG [H]

Identifier: 167040440

GI number: 167040440

Start: 1814404

End: 1815126

Strand: Direct

Name: ytzG [H]

Synonym: Teth514_1807

Alternate gene names: 167040440

Gene position: 1814404-1815126 (Clockwise)

Preceding gene: 167040439

Following gene: 167040444

Centisome position: 73.84

GC content: 31.81

Gene sequence:

>723_bases
ATGGCAAAAATGAGAATAGATAAACTTTTATCGAATATAGGCATTGGTACCAGAAAAGAGGTTAAAAAATTCATTAAAGA
AGGTCTTGTATTGGTAAATGGAAATACAGTAAAAGATGCTGGTTTAATTATCAACACTGAAACTGACGAGATTCTATTTG
ATGGCAAAAAAATCTATTACAAGGAATTTATATATATTATGATGAATAAACCAGAAGGAGTTATTAGCGCTACTTATGAT
CCTATAGAAAAAACTGTTATTGACTTATTACCTCAAGAATTAAAAGCTCGAAATCCCTTTCCAGTAGGAAGACTTGATAA
AGATACAGAAGGATTACTTCTTATAACTAATGACGGAGATTTGGCACATAAGCTTTTATCTCCAAAAAAGAATGTTATAA
AAAAATATTATGCTAAAATATTAGGCTTTGTCAATGAAAGCGACATTAAAGCTTTCAATGAAGGCATTGTACTTGAAGAT
GGGTATAAAACTCTTCCCGCAAACCTCGAAATTCTTTCTTCTTCTGATGTGTCGAAAGTTTACGTTTATATAAGAGAAGG
AAAATACCACCAAATAAAAAGAATGTTTGAATCAGTCGGTAAAAAGGTCATATACCTTAAAAGATTGGCAATGGGTAATC
TCACCCTTGACGAAAATCTAAAACCTGGGGAGTGGAGAGAATTGTCTGAAGAAGAGCTATCTTTCCTGAAAAAGTCGATA
TAA

Upstream 100 bases:

>100_bases
GTTTAAAGTAAGTTGAATAAACCGACAATTTTCATGTACTCTAATAACAAAAAGTTTTGTGTTATAATCACAATATAGAA
TAAGTGCGGAGGTAATTTAT

Downstream 100 bases:

>100_bases
AAAATAAGACTTTCACCAAAGCCTTTCAAATAAATCCTCCGTCTACTGTTATGACTTGCCCTGTTATGAAGTTTGCTTTG
TCAGATAATAAAAACAATAC

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MAKMRIDKLLSNIGIGTRKEVKKFIKEGLVLVNGNTVKDAGLIINTETDEILFDGKKIYYKEFIYIMMNKPEGVISATYD
PIEKTVIDLLPQELKARNPFPVGRLDKDTEGLLLITNDGDLAHKLLSPKKNVIKKYYAKILGFVNESDIKAFNEGIVLED
GYKTLPANLEILSSSDVSKVYVYIREGKYHQIKRMFESVGKKVIYLKRLAMGNLTLDENLKPGEWRELSEEELSFLKKSI

Sequences:

>Translated_240_residues
MAKMRIDKLLSNIGIGTRKEVKKFIKEGLVLVNGNTVKDAGLIINTETDEILFDGKKIYYKEFIYIMMNKPEGVISATYD
PIEKTVIDLLPQELKARNPFPVGRLDKDTEGLLLITNDGDLAHKLLSPKKNVIKKYYAKILGFVNESDIKAFNEGIVLED
GYKTLPANLEILSSSDVSKVYVYIREGKYHQIKRMFESVGKKVIYLKRLAMGNLTLDENLKPGEWRELSEEELSFLKKSI
>Mature_239_residues
AKMRIDKLLSNIGIGTRKEVKKFIKEGLVLVNGNTVKDAGLIINTETDEILFDGKKIYYKEFIYIMMNKPEGVISATYDP
IEKTVIDLLPQELKARNPFPVGRLDKDTEGLLLITNDGDLAHKLLSPKKNVIKKYYAKILGFVNESDIKAFNEGIVLEDG
YKTLPANLEILSSSDVSKVYVYIREGKYHQIKRMFESVGKKVIYLKRLAMGNLTLDENLKPGEWRELSEEELSFLKKSI

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=36.3247863247863, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=31.9672131147541, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=28.1512605042017, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=34.0659340659341, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27337; Mature: 27206

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRIDKLLSNIGIGTRKEVKKFIKEGLVLVNGNTVKDAGLIINTETDEILFDGKKIYY
CCCHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCEEECCCEEEECCCCCEEECCCEEEE
KEFIYIMMNKPEGVISATYDPIEKTVIDLLPQELKARNPFPVGRLDKDTEGLLLITNDGD
EEEEEEEECCCCCEEECCCCHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCEEEEECCCH
LAHKLLSPKKNVIKKYYAKILGFVNESDIKAFNEGIVLEDGYKTLPANLEILSSSDVSKV
HHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEECCCEECCCCEEEECCCCCEEE
YVYIREGKYHQIKRMFESVGKKVIYLKRLAMGNLTLDENLKPGEWRELSEEELSFLKKSI
EEEEECCCHHHHHHHHHHHCHHEEHHHHHHHCCCEECCCCCCCCCHHCCHHHHHHHHHCC
>Mature Secondary Structure 
AKMRIDKLLSNIGIGTRKEVKKFIKEGLVLVNGNTVKDAGLIINTETDEILFDGKKIYY
CCHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCEEECCCEEEECCCCCEEECCCEEEE
KEFIYIMMNKPEGVISATYDPIEKTVIDLLPQELKARNPFPVGRLDKDTEGLLLITNDGD
EEEEEEEECCCCCEEECCCCHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCEEEEECCCH
LAHKLLSPKKNVIKKYYAKILGFVNESDIKAFNEGIVLEDGYKTLPANLEILSSSDVSKV
HHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEECCCEECCCCEEEECCCCCEEE
YVYIREGKYHQIKRMFESVGKKVIYLKRLAMGNLTLDENLKPGEWRELSEEELSFLKKSI
EEEEECCCHHHHHHHHHHHCHHEEHHHHHHHCCCEECCCCCCCCCHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]