Click here to switch to the map view.

The map label for this gene is rlmI [H]

Identifier: 167040444

GI number: 167040444

Start: 1822341

End: 1823588

Strand: Direct

Name: rlmI [H]

Synonym: Teth514_1811

Alternate gene names: 167040444

Gene position: 1822341-1823588 (Clockwise)

Preceding gene: 167040440

Following gene: 167040445

Centisome position: 74.16

GC content: 32.05

Gene sequence:

>1248_bases
ATGTCAATACAATTGAATATTTTAGCTAATTATGATATAAAGAAATTATTAGAAGTTGTGAGGAGAGAAGTTATGGCAGA
TGTTATATTAAGAAACACAAATATTAAAAGGATTTTAAATGGTCACCCTTGGATATACAAAACTGAAATAGATAGAATTG
AAGGAGATTACACTCCAGGTGGAATTGTAAATGTTTTAAATCACAAAAAAGAATTCATAGGAAAAGGCTATATAAATTTA
AAATCCATGATAACAGTAAGACTATTGACTCGGGATATTCATGAAGAAATAGATGAAGAATTTTTTAGAAAAAGAATTAA
AAGAGCCTGGGAATACAGAAAAAAGGTTATGGACAATTTAAACTCCTGTAGAGTCGTATTTGCAGAAGCAGATTTTTTAC
CTGCTTTAATCGTGGACAAATTTGGTGACTATCTCGTTCTTCAAACACTTTCATTAGGTATAGATCGATATAAAGAAACA
ATAGTAAAGCTTCTTGTTGAAATATTAAATCCCAAAGGAATTTATGAAAGAAATGATGTAAATGTAAGAGAATTAGAGGG
CCTTCCACAGCAAAAAGGTTATTTGTACGGCAAGTTTGACACAATGCAACAATTTGAAGAAAATGGAATAAAATTTTGGG
TAGACATTGAAAATGGCCAAAAGACTGGATATTTTTTAGATCAAAAAGAAAACAGAAGAGCAATTCAAAATTACGTCAAA
GATGCAGAAGTTCTTGATTGTTTCAGCCACACTGGTTCATTTACAGTACATGCCCTACACTATGGAGCAAAAAGAGTCGA
GACAGTCGACATCTCTGAGGAAGCCATTGAAATGGCCAAAAAGAATGTAGAACTCAATGGTTATCAAGAAAGATGTAATT
TCGTATGTGACAATGCCTTTGATTTGCTGAGGAAATACGACAAAGAAAATAGAAAATTTGACACTGTAATATTAGACCCT
CCTGCCTTTACAAAAAGCAAAGAAACTGTAAAAGACGCATTAAGGGGATACAAGGAAATAAATTTAAGAGCATTAAAAAT
TCTAAGAGAAGGAGGCTTTCTCATCACCTGTTCTTGTTCACAGCACATAAAACCTGATATGTTTTTAGATGTCATTAAAG
AAGCAGCCAATGATGCTAAAAGAAATGTAAGACTTGTAGAACAACGTACTCAATCAAAAGACCATCCAATATTATTAGCC
TCTGAAGAAACTCAATACTTAAAGTGCCTAATTCTACAAGTCTTATAA

Upstream 100 bases:

>100_bases
CCCTCTTGTATCATATTAAAATCTTGAACTGCTCTTCTTATTCTTCCAAGTATTTTTTGCATATTATCACCCCTTCAATA
TTAATACTATTCACAGGAAT

Downstream 100 bases:

>100_bases
AAAAGAACACTTTCCCTATTTTTATCATATTGTGTAATAGTGAAAGTGTATAAATGAGGTGAAAAAATGGAGAGGCAACA
ATTAGGCTCCAGGCTTCTAT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSIQLNILANYDIKKLLEVVRREVMADVILRNTNIKRILNGHPWIYKTEIDRIEGDYTPGGIVNVLNHKKEFIGKGYINL
KSMITVRLLTRDIHEEIDEEFFRKRIKRAWEYRKKVMDNLNSCRVVFAEADFLPALIVDKFGDYLVLQTLSLGIDRYKET
IVKLLVEILNPKGIYERNDVNVRELEGLPQQKGYLYGKFDTMQQFEENGIKFWVDIENGQKTGYFLDQKENRRAIQNYVK
DAEVLDCFSHTGSFTVHALHYGAKRVETVDISEEAIEMAKKNVELNGYQERCNFVCDNAFDLLRKYDKENRKFDTVILDP
PAFTKSKETVKDALRGYKEINLRALKILREGGFLITCSCSQHIKPDMFLDVIKEAANDAKRNVRLVEQRTQSKDHPILLA
SEETQYLKCLILQVL

Sequences:

>Translated_415_residues
MSIQLNILANYDIKKLLEVVRREVMADVILRNTNIKRILNGHPWIYKTEIDRIEGDYTPGGIVNVLNHKKEFIGKGYINL
KSMITVRLLTRDIHEEIDEEFFRKRIKRAWEYRKKVMDNLNSCRVVFAEADFLPALIVDKFGDYLVLQTLSLGIDRYKET
IVKLLVEILNPKGIYERNDVNVRELEGLPQQKGYLYGKFDTMQQFEENGIKFWVDIENGQKTGYFLDQKENRRAIQNYVK
DAEVLDCFSHTGSFTVHALHYGAKRVETVDISEEAIEMAKKNVELNGYQERCNFVCDNAFDLLRKYDKENRKFDTVILDP
PAFTKSKETVKDALRGYKEINLRALKILREGGFLITCSCSQHIKPDMFLDVIKEAANDAKRNVRLVEQRTQSKDHPILLA
SEETQYLKCLILQVL
>Mature_414_residues
SIQLNILANYDIKKLLEVVRREVMADVILRNTNIKRILNGHPWIYKTEIDRIEGDYTPGGIVNVLNHKKEFIGKGYINLK
SMITVRLLTRDIHEEIDEEFFRKRIKRAWEYRKKVMDNLNSCRVVFAEADFLPALIVDKFGDYLVLQTLSLGIDRYKETI
VKLLVEILNPKGIYERNDVNVRELEGLPQQKGYLYGKFDTMQQFEENGIKFWVDIENGQKTGYFLDQKENRRAIQNYVKD
AEVLDCFSHTGSFTVHALHYGAKRVETVDISEEAIEMAKKNVELNGYQERCNFVCDNAFDLLRKYDKENRKFDTVILDPP
AFTKSKETVKDALRGYKEINLRALKILREGGFLITCSCSQHIKPDMFLDVIKEAANDAKRNVRLVEQRTQSKDHPILLAS
EETQYLKCLILQVL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=392, Percent_Identity=38.5204081632653, Blast_Score=307, Evalue=6e-85,
Organism=Escherichia coli, GI1787181, Length=266, Percent_Identity=28.9473684210526, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF01472 PUA [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 48388; Mature: 48256

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQLNILANYDIKKLLEVVRREVMADVILRNTNIKRILNGHPWIYKTEIDRIEGDYTPG
CEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEHHHCCCCCCCC
GIVNVLNHKKEFIGKGYINLKSMITVRLLTRDIHEEIDEEFFRKRIKRAWEYRKKVMDNL
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NSCRVVFAEADFLPALIVDKFGDYLVLQTLSLGIDRYKETIVKLLVEILNPKGIYERNDV
CCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NVRELEGLPQQKGYLYGKFDTMQQFEENGIKFWVDIENGQKTGYFLDQKENRRAIQNYVK
CHHHHCCCCCCCCEEEECHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCHHHHHHHHH
DAEVLDCFSHTGSFTVHALHYGAKRVETVDISEEAIEMAKKNVELNGYQERCNFVCDNAF
HHHHHHHHHCCCCEEEEEEHHCCHHEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCHHH
DLLRKYDKENRKFDTVILDPPAFTKSKETVKDALRGYKEINLRALKILREGGFLITCSCS
HHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
QHIKPDMFLDVIKEAANDAKRNVRLVEQRTQSKDHPILLASEETQYLKCLILQVL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHC
>Mature Secondary Structure 
SIQLNILANYDIKKLLEVVRREVMADVILRNTNIKRILNGHPWIYKTEIDRIEGDYTPG
EEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEHHHCCCCCCCC
GIVNVLNHKKEFIGKGYINLKSMITVRLLTRDIHEEIDEEFFRKRIKRAWEYRKKVMDNL
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NSCRVVFAEADFLPALIVDKFGDYLVLQTLSLGIDRYKETIVKLLVEILNPKGIYERNDV
CCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NVRELEGLPQQKGYLYGKFDTMQQFEENGIKFWVDIENGQKTGYFLDQKENRRAIQNYVK
CHHHHCCCCCCCCEEEECHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCHHHHHHHHH
DAEVLDCFSHTGSFTVHALHYGAKRVETVDISEEAIEMAKKNVELNGYQERCNFVCDNAF
HHHHHHHHHCCCCEEEEEEHHCCHHEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCHHH
DLLRKYDKENRKFDTVILDPPAFTKSKETVKDALRGYKEINLRALKILREGGFLITCSCS
HHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
QHIKPDMFLDVIKEAANDAKRNVRLVEQRTQSKDHPILLASEETQYLKCLILQVL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA