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The map label for this gene is priA [H]

Identifier: 167040395

GI number: 167040395

Start: 1761901

End: 1764099

Strand: Reverse

Name: priA [H]

Synonym: Teth514_1758

Alternate gene names: 167040395

Gene position: 1764099-1761901 (Counterclockwise)

Preceding gene: 167040396

Following gene: 167040394

Centisome position: 71.79

GC content: 33.88

Gene sequence:

>2199_bases
ATGTTTGCCGAGGTCGTTGTAGACATTAAATCTTCTAATACTGACAGGGTATATACTTATCGTATTCCTGAAGGAATGAA
TGTAAAAATTGGGATGAGGGTATCTGTCCCCTTTAATAATAGGTTTATAGAGGGATATGTAATAAGTATTACTGACAAGG
CGTCTTATTCAGTGGATAAAATAAAGGATATCCATAGGATTTTAGACAGCTATAGCATATTTGATGAAAAAATGATTGAA
CTGGCTAAGTGGATGAAGGATTATTATAAATGTTATTTTTCTGAAGCTCTCCAGACTATAATGCCTGTAGGGATAAAGCA
AGGGGAAAAGAAAGTAAAGGTTGTGAAAATAAATATTGATGAGATACCTTCACAGCTTTCCAAAAAGGCGCCAAAGCAAT
ACGAAATATTACAATATTTAAAAAATAGAAGTCCTATTAGGCTTTCTGAGCTTGTCAAGGTGCTCAATATAGACTATGGA
GTTATAAAAAGTTTACAAAATAAAGGGTATATAGAAATTTACGAGGAAGAAGTATTGAGATTTAGTGTAAAAGATATTGA
AAAAACTAAGCCTCTATTATTGACAGAAGAACAACAAAGAGCTGTAGAAGAGGTTAAAAGGTCTATCTTGACAGGCACTT
TTGATAAATATTTGCTTTTTGGTGTAACAGGAAGTGGCAAAACTGAAGTTTATCTTCAATTGATAGATGAAGCTATAAAA
ATAGGCAAAAGCGCTATAGTTTTAGTACCAGAAATCTCATTGACGCCTCAGACTATTGAAAGGTTTGTGAGTCGATTTGG
CAATAGAGTGGCTGTAATTCACAGTGGCCTTTCACCGGGAGAACGTTTTGATGAATGGAGAAAAGTAAAAAATGGTTTAG
TGGACGTAGTAGTTGGTGTGAGAAATGCAGTTTTTGCTCCTTTTAATAATCTTGGCCTCATTATAATTGACGAGGAACAT
GAGACCACCTACAAACAATCAGACTTAAGACCTAAATATAATGCTAAAGAAGTGGCAGAAAAGCGGTGTGAAATAGAAAA
AGCAGTTCTGGTTATGGCCAGTGCTACTCCTTCTTTAGAGACCTATTATAAAGCTAATAAAGGAGAATATAAACTTATAA
GACTTACAAAAAGGATTAATGTTTCTATGCCGCAGATAGAAGTTGTCAATATGAGTGAGGAATTGGCAAGCGGAAATACT
TCAATATTTAGTCGCAAATTATTTTCCTATATAAAAGAAAATCTTAAAAGGAAAGAGCAAACTATTCTTTTTTTAAACAG
AAGAGGATACTCTTCATTTGTTTCCTGCAGAGATTGCGGTTATGTGCCTAAATGTCCTAATTGTGATATTTCTTTAACTT
ATCATTTTGAAGATAAAAAATTGGTTTGCCATTATTGTGGTTATGAGGAGACTATGAAAGATACATGTCCTAAATGTGGA
AGTAAAAGGATTAGGTATTTAGGCATAGGGACCGAAAGGGTAGAAAACGACATAAAAAAGTTTTTTCCTAATGCAAGAGT
TTTGAGGATGGATGTAGATACCACAAGAAAAAAAGGGTCTCATGAGAAAATTTTTTATGACTTTAGAAATGGTAAGGCAG
ACATTCTAATCGGTACGCAAATGATATCTAAAGGCTTTGACATTCCAAATGTTACGTTGGTGGGAGTAATACTTGCAGAT
ATAACTTTAAATCTTCCTGATTTTAGGTCCAGTGAGAGGACTTTTCAGCTGCTCACTCAGGTAGCTGGAAGGGCAGGAAG
AGGTGAAAAGCCTGGAAGAGTAGTAATACAGACCTATGAAGATGACCACTATAGTATAGTTACTGCAAAACAGCAAAATT
ATGTGAAGTTTTACAACGAAGAGATAAAATACAGAGAAATTTTTAGATACCCTCCTTTTTCACATCTAATGAATATAGTG
GTATCAGGAGAAAAGGAGGAAGAGGTTAAAAATACTGCTGTAAGCACTTATTTAACTTGTCAAAAGCTTATTAACAAACT
TCAAAATAAGAGTTATAATAAAATATTAGGACCTGCTCCAGCTCCTATAAGTAAAATAAACAACCGATATAGATGGCAGG
TCATTTTAAAAAGTGAAGATAGAGACATCTTGACGGAGATAGCAGAAGAAATTCAAAAGATGAAATATTCTAAAGATATA
AGGATAGCTGTAGACATTGACCCTTTGAGTATAATGTAA

Upstream 100 bases:

>100_bases
AGATAGAAGCTTCTCATGTGATATTAGATGAAATAGCTAAATTGTTGTAAGGCCTTTCGGCTTTTTTTATTTGGTAAATA
TTTAACAGGCGGAGGTTGCC

Downstream 100 bases:

>100_bases
ATTTAAGGAGGTATTTATTTTGGCAATAAGGTATGTTAGAAAAATTGGAGATGAGGTCTTAAGAAAAAAAGCAAAACCTG
TTACAGAGATAAATTCTCAT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MFAEVVVDIKSSNTDRVYTYRIPEGMNVKIGMRVSVPFNNRFIEGYVISITDKASYSVDKIKDIHRILDSYSIFDEKMIE
LAKWMKDYYKCYFSEALQTIMPVGIKQGEKKVKVVKINIDEIPSQLSKKAPKQYEILQYLKNRSPIRLSELVKVLNIDYG
VIKSLQNKGYIEIYEEEVLRFSVKDIEKTKPLLLTEEQQRAVEEVKRSILTGTFDKYLLFGVTGSGKTEVYLQLIDEAIK
IGKSAIVLVPEISLTPQTIERFVSRFGNRVAVIHSGLSPGERFDEWRKVKNGLVDVVVGVRNAVFAPFNNLGLIIIDEEH
ETTYKQSDLRPKYNAKEVAEKRCEIEKAVLVMASATPSLETYYKANKGEYKLIRLTKRINVSMPQIEVVNMSEELASGNT
SIFSRKLFSYIKENLKRKEQTILFLNRRGYSSFVSCRDCGYVPKCPNCDISLTYHFEDKKLVCHYCGYEETMKDTCPKCG
SKRIRYLGIGTERVENDIKKFFPNARVLRMDVDTTRKKGSHEKIFYDFRNGKADILIGTQMISKGFDIPNVTLVGVILAD
ITLNLPDFRSSERTFQLLTQVAGRAGRGEKPGRVVIQTYEDDHYSIVTAKQQNYVKFYNEEIKYREIFRYPPFSHLMNIV
VSGEKEEEVKNTAVSTYLTCQKLINKLQNKSYNKILGPAPAPISKINNRYRWQVILKSEDRDILTEIAEEIQKMKYSKDI
RIAVDIDPLSIM

Sequences:

>Translated_732_residues
MFAEVVVDIKSSNTDRVYTYRIPEGMNVKIGMRVSVPFNNRFIEGYVISITDKASYSVDKIKDIHRILDSYSIFDEKMIE
LAKWMKDYYKCYFSEALQTIMPVGIKQGEKKVKVVKINIDEIPSQLSKKAPKQYEILQYLKNRSPIRLSELVKVLNIDYG
VIKSLQNKGYIEIYEEEVLRFSVKDIEKTKPLLLTEEQQRAVEEVKRSILTGTFDKYLLFGVTGSGKTEVYLQLIDEAIK
IGKSAIVLVPEISLTPQTIERFVSRFGNRVAVIHSGLSPGERFDEWRKVKNGLVDVVVGVRNAVFAPFNNLGLIIIDEEH
ETTYKQSDLRPKYNAKEVAEKRCEIEKAVLVMASATPSLETYYKANKGEYKLIRLTKRINVSMPQIEVVNMSEELASGNT
SIFSRKLFSYIKENLKRKEQTILFLNRRGYSSFVSCRDCGYVPKCPNCDISLTYHFEDKKLVCHYCGYEETMKDTCPKCG
SKRIRYLGIGTERVENDIKKFFPNARVLRMDVDTTRKKGSHEKIFYDFRNGKADILIGTQMISKGFDIPNVTLVGVILAD
ITLNLPDFRSSERTFQLLTQVAGRAGRGEKPGRVVIQTYEDDHYSIVTAKQQNYVKFYNEEIKYREIFRYPPFSHLMNIV
VSGEKEEEVKNTAVSTYLTCQKLINKLQNKSYNKILGPAPAPISKINNRYRWQVILKSEDRDILTEIAEEIQKMKYSKDI
RIAVDIDPLSIM
>Mature_732_residues
MFAEVVVDIKSSNTDRVYTYRIPEGMNVKIGMRVSVPFNNRFIEGYVISITDKASYSVDKIKDIHRILDSYSIFDEKMIE
LAKWMKDYYKCYFSEALQTIMPVGIKQGEKKVKVVKINIDEIPSQLSKKAPKQYEILQYLKNRSPIRLSELVKVLNIDYG
VIKSLQNKGYIEIYEEEVLRFSVKDIEKTKPLLLTEEQQRAVEEVKRSILTGTFDKYLLFGVTGSGKTEVYLQLIDEAIK
IGKSAIVLVPEISLTPQTIERFVSRFGNRVAVIHSGLSPGERFDEWRKVKNGLVDVVVGVRNAVFAPFNNLGLIIIDEEH
ETTYKQSDLRPKYNAKEVAEKRCEIEKAVLVMASATPSLETYYKANKGEYKLIRLTKRINVSMPQIEVVNMSEELASGNT
SIFSRKLFSYIKENLKRKEQTILFLNRRGYSSFVSCRDCGYVPKCPNCDISLTYHFEDKKLVCHYCGYEETMKDTCPKCG
SKRIRYLGIGTERVENDIKKFFPNARVLRMDVDTTRKKGSHEKIFYDFRNGKADILIGTQMISKGFDIPNVTLVGVILAD
ITLNLPDFRSSERTFQLLTQVAGRAGRGEKPGRVVIQTYEDDHYSIVTAKQQNYVKFYNEEIKYREIFRYPPFSHLMNIV
VSGEKEEEVKNTAVSTYLTCQKLINKLQNKSYNKILGPAPAPISKINNRYRWQVILKSEDRDILTEIAEEIQKMKYSKDI
RIAVDIDPLSIM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=743, Percent_Identity=34.7240915208614, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 84313; Mature: 84313

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAEVVVDIKSSNTDRVYTYRIPEGMNVKIGMRVSVPFNNRFIEGYVISITDKASYSVDK
CCCEEEEEEECCCCCEEEEEECCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCHHH
IKDIHRILDSYSIFDEKMIELAKWMKDYYKCYFSEALQTIMPVGIKQGEKKVKVVKINID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHH
EIPSQLSKKAPKQYEILQYLKNRSPIRLSELVKVLNIDYGVIKSLQNKGYIEIYEEEVLR
HHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCEEEEHHHHHH
FSVKDIEKTKPLLLTEEQQRAVEEVKRSILTGTFDKYLLFGVTGSGKTEVYLQLIDEAIK
HHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
IGKSAIVLVPEISLTPQTIERFVSRFGNRVAVIHSGLSPGERFDEWRKVKNGLVDVVVGV
CCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHH
RNAVFAPFNNLGLIIIDEEHETTYKQSDLRPKYNAKEVAEKRCEIEKAVLVMASATPSLE
HHHHCCCCCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHH
TYYKANKGEYKLIRLTKRINVSMPQIEVVNMSEELASGNTSIFSRKLFSYIKENLKRKEQ
HHEECCCCCEEEEEEEHHCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
TILFLNRRGYSSFVSCRDCGYVPKCPNCDISLTYHFEDKKLVCHYCGYEETMKDTCPKCG
EEEEEECCCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHCCCCC
SKRIRYLGIGTERVENDIKKFFPNARVLRMDVDTTRKKGSHEKIFYDFRNGKADILIGTQ
CCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCHHCCCCCCCEEEEEECCCCEEEEEEHH
MISKGFDIPNVTLVGVILADITLNLPDFRSSERTFQLLTQVAGRAGRGEKPGRVVIQTYE
HHHCCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
DDHYSIVTAKQQNYVKFYNEEIKYREIFRYPPFSHLMNIVVSGEKEEEVKNTAVSTYLTC
CCCEEEEEECCCCHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QKLINKLQNKSYNKILGPAPAPISKINNRYRWQVILKSEDRDILTEIAEEIQKMKYSKDI
HHHHHHHHCCCCCCCCCCCCCCHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCE
RIAVDIDPLSIM
EEEEECCCCCCC
>Mature Secondary Structure
MFAEVVVDIKSSNTDRVYTYRIPEGMNVKIGMRVSVPFNNRFIEGYVISITDKASYSVDK
CCCEEEEEEECCCCCEEEEEECCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCHHH
IKDIHRILDSYSIFDEKMIELAKWMKDYYKCYFSEALQTIMPVGIKQGEKKVKVVKINID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHH
EIPSQLSKKAPKQYEILQYLKNRSPIRLSELVKVLNIDYGVIKSLQNKGYIEIYEEEVLR
HHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCEEEEHHHHHH
FSVKDIEKTKPLLLTEEQQRAVEEVKRSILTGTFDKYLLFGVTGSGKTEVYLQLIDEAIK
HHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
IGKSAIVLVPEISLTPQTIERFVSRFGNRVAVIHSGLSPGERFDEWRKVKNGLVDVVVGV
CCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHH
RNAVFAPFNNLGLIIIDEEHETTYKQSDLRPKYNAKEVAEKRCEIEKAVLVMASATPSLE
HHHHCCCCCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHH
TYYKANKGEYKLIRLTKRINVSMPQIEVVNMSEELASGNTSIFSRKLFSYIKENLKRKEQ
HHEECCCCCEEEEEEEHHCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
TILFLNRRGYSSFVSCRDCGYVPKCPNCDISLTYHFEDKKLVCHYCGYEETMKDTCPKCG
EEEEEECCCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHCCCCC
SKRIRYLGIGTERVENDIKKFFPNARVLRMDVDTTRKKGSHEKIFYDFRNGKADILIGTQ
CCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCHHCCCCCCCEEEEEECCCCEEEEEEHH
MISKGFDIPNVTLVGVILADITLNLPDFRSSERTFQLLTQVAGRAGRGEKPGRVVIQTYE
HHHCCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
DDHYSIVTAKQQNYVKFYNEEIKYREIFRYPPFSHLMNIVVSGEKEEEVKNTAVSTYLTC
CCCEEEEEECCCCHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QKLINKLQNKSYNKILGPAPAPISKINNRYRWQVILKSEDRDILTEIAEEIQKMKYSKDI
HHHHHHHHCCCCCCCCCCCCCCHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCE
RIAVDIDPLSIM
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]