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The map label for this gene is def

Identifier: 167040394

GI number: 167040394

Start: 1761381

End: 1761881

Strand: Reverse

Name: def

Synonym: Teth514_1757

Alternate gene names: 167040394

Gene position: 1761881-1761381 (Counterclockwise)

Preceding gene: 167040395

Following gene: 167040392

Centisome position: 71.7

GC content: 35.73

Gene sequence:

>501_bases
TTGGCAATAAGGTATGTTAGAAAAATTGGAGATGAGGTCTTAAGAAAAAAAGCAAAACCTGTTACAGAGATAAATTCTCA
TATTTTGACTATATTAGAGGATATGGCACAAACTATGTATCTCAACGATGGAGTAGGATTGGCTGCTAATCAAATAGGAG
TGTTGAGAAGGTTAGTTGTCATAGATGTGGGAGAAGGTCTTTTAGAGCTTATAAACCCTGAGATTGTATATGAAGAAGGA
GAACAAGTAGGGGCCGAAGGATGTTTAAGTATTCCGGGAGTAGTAGGAGAGGTTAAAAGGCCTAAAAAAGTAAAGGTAAA
ATATTTGGATAGAGAAGGTAAAGAGAGGGAAATTGAAGGTGAGGATTTACTGGCAAGAGCTTTATGCCATGAAATAGACC
ATTTAAACGGGGTTTTGTTTATAGATAAGGCTATAAGATTTTTAGATGAAGAAGAAAAAGAACAGGTTAAGTATTTCGGT
AATATGTCAAGAGGGTGGTAA

Upstream 100 bases:

>100_bases
ATAGCAGAAGAAATTCAAAAGATGAAATATTCTAAAGATATAAGGATAGCTGTAGACATTGACCCTTTGAGTATAATGTA
AATTTAAGGAGGTATTTATT

Downstream 100 bases:

>100_bases
AAAAAATTCAAAAAAAATTTAATAAAATAACCTAAAAAAGGGTATAGCAAAGCTAACCATCACGCAATAAGCTAATGGAA
ATTAATGTAAAATATGGTTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MAIRYVRKIGDEVLRKKAKPVTEINSHILTILEDMAQTMYLNDGVGLAANQIGVLRRLVVIDVGEGLLELINPEIVYEEG
EQVGAEGCLSIPGVVGEVKRPKKVKVKYLDREGKEREIEGEDLLARALCHEIDHLNGVLFIDKAIRFLDEEEKEQVKYFG
NMSRGW

Sequences:

>Translated_166_residues
MAIRYVRKIGDEVLRKKAKPVTEINSHILTILEDMAQTMYLNDGVGLAANQIGVLRRLVVIDVGEGLLELINPEIVYEEG
EQVGAEGCLSIPGVVGEVKRPKKVKVKYLDREGKEREIEGEDLLARALCHEIDHLNGVLFIDKAIRFLDEEEKEQVKYFG
NMSRGW
>Mature_165_residues
AIRYVRKIGDEVLRKKAKPVTEINSHILTILEDMAQTMYLNDGVGLAANQIGVLRRLVVIDVGEGLLELINPEIVYEEGE
QVGAEGCLSIPGVVGEVKRPKKVKVKYLDREGKEREIEGEDLLARALCHEIDHLNGVLFIDKAIRFLDEEEKEQVKYFGN
MSRGW

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=29.6296296296296, Blast_Score=71, Evalue=5e-13,
Organism=Escherichia coli, GI1789682, Length=145, Percent_Identity=49.6551724137931, Blast_Score=130, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24645728, Length=164, Percent_Identity=35.3658536585366, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24645726, Length=166, Percent_Identity=31.3253012048193, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_THEPX (B0K292)

Other databases:

- EMBL:   CP000923
- RefSeq:   YP_001663379.1
- ProteinModelPortal:   B0K292
- SMR:   B0K292
- GeneID:   5876841
- GenomeReviews:   CP000923_GR
- KEGG:   tex:Teth514_1757
- HOGENOM:   HBG665227
- OMA:   EINSHIL
- ProtClustDB:   PRK00150
- BioCyc:   TSP1755:TETH514_1757-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 18745; Mature: 18614

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRYVRKIGDEVLRKKAKPVTEINSHILTILEDMAQTMYLNDGVGLAANQIGVLRRLVV
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IDVGEGLLELINPEIVYEEGEQVGAEGCLSIPGVVGEVKRPKKVKVKYLDREGKEREIEG
HHCCHHHHHHHCCHHEECCCHHCCCCCCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCCH
EDLLARALCHEIDHLNGVLFIDKAIRFLDEEEKEQVKYFGNMSRGW
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AIRYVRKIGDEVLRKKAKPVTEINSHILTILEDMAQTMYLNDGVGLAANQIGVLRRLVV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IDVGEGLLELINPEIVYEEGEQVGAEGCLSIPGVVGEVKRPKKVKVKYLDREGKEREIEG
HHCCHHHHHHHCCHHEECCCHHCCCCCCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCCH
EDLLARALCHEIDHLNGVLFIDKAIRFLDEEEKEQVKYFGNMSRGW
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA