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The map label for this gene is miaA

Identifier: 167040250

GI number: 167040250

Start: 1631257

End: 1632204

Strand: Direct

Name: miaA

Synonym: Teth514_1613

Alternate gene names: 167040250

Gene position: 1631257-1632204 (Clockwise)

Preceding gene: 167040249

Following gene: 167040251

Centisome position: 66.39

GC content: 31.22

Gene sequence:

>948_bases
ATGGCTATACAAATTGTGCTTATTGTTGGGCCCACAGCCTCTGGCAAGTCTAAATTAGCAGTAGACGTGGCAAAAGAATT
TAATGGAGAAATCATTTCTGCTGACTCTATGCAAGTTTATAAGTACATGGATGTTGGTACAGCAAAAATTACAAAGGAAG
AAATGCAAGGCATCCCTCATTATTTGATAGATATTGTAGAGCCAGATCAAGAGTTTAGTGTAGCAGAATATGAAAAAAGA
GCGAAAGAGATTATAAAGGATATTTACAAAAGAGGGAAACTTCCTATAATTGTAGGGGGAACTGGTTTATATATCAATTC
AATAATTTACATCATGCACTTTTCTGATTTTGAAGGAAGCAAAGAATTTAGAGAGAAAATGAAAGAATTAGCAAATACTT
ATGGCAGTCAATATCTTTATGAAAAATTAAAAAGTGTGGACCCAGAGGCGGCAAAAAAAATACATCCAAATGATATTAGA
AGAATCATCAGAGCTTTAGAAGTTTATGAATTTACAGGGAAACCTATTTCTTACTATCAAAAAATGAGTGGTATGCGTTT
AAATCCAGAGTATCAGCCTATAATGATAGGGCTTAATTTCAGAGACAGACAAATTTTATACGATAGAATTAATCAAAGAG
TGGATGAAATGATAAAAAATAATTTGGTTGAAGAAGTGGTAAACTTGCTAAAAATCGGGTATAATAAAGACAGTACTGCT
ATGCAGGCTTTAGGATATAAAGAAATTGTCGAATATTTAAAAGGAGAGATTTCTTTAGAAGAAGCTATTGAAAAAATTAA
AAAAGGGACAAGGAGGTATGCAAAAAGACAGATCACCTGGTTTAAGGGTTATGACTTTATCAAATGGTTTTTTGTAGATG
ACTATAAAAATTATGAAGAACTCAAGAAAAATATTATTAAATATTTAGCAGGAAAATTAAATTTTTGA

Upstream 100 bases:

>100_bases
TCCTTATACTTGTCCTCACGGAAGACCTGTTATAATCGCTATAACAAAAACTCAGTTAGAAAAGATGTTTAAAAGAATTA
TGTAATATAAGGGTGATGGG

Downstream 100 bases:

>100_bases
TAGAATATAACTAATGATACATATTAAAGAAGGGGGATAAAGATGGCAAGTTCAAAAGCAGCTATTAATTTACAGGATAT
CTTTTTAAATCAAGTGAGGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MAIQIVLIVGPTASGKSKLAVDVAKEFNGEIISADSMQVYKYMDVGTAKITKEEMQGIPHYLIDIVEPDQEFSVAEYEKR
AKEIIKDIYKRGKLPIIVGGTGLYINSIIYIMHFSDFEGSKEFREKMKELANTYGSQYLYEKLKSVDPEAAKKIHPNDIR
RIIRALEVYEFTGKPISYYQKMSGMRLNPEYQPIMIGLNFRDRQILYDRINQRVDEMIKNNLVEEVVNLLKIGYNKDSTA
MQALGYKEIVEYLKGEISLEEAIEKIKKGTRRYAKRQITWFKGYDFIKWFFVDDYKNYEELKKNIIKYLAGKLNF

Sequences:

>Translated_315_residues
MAIQIVLIVGPTASGKSKLAVDVAKEFNGEIISADSMQVYKYMDVGTAKITKEEMQGIPHYLIDIVEPDQEFSVAEYEKR
AKEIIKDIYKRGKLPIIVGGTGLYINSIIYIMHFSDFEGSKEFREKMKELANTYGSQYLYEKLKSVDPEAAKKIHPNDIR
RIIRALEVYEFTGKPISYYQKMSGMRLNPEYQPIMIGLNFRDRQILYDRINQRVDEMIKNNLVEEVVNLLKIGYNKDSTA
MQALGYKEIVEYLKGEISLEEAIEKIKKGTRRYAKRQITWFKGYDFIKWFFVDDYKNYEELKKNIIKYLAGKLNF
>Mature_314_residues
AIQIVLIVGPTASGKSKLAVDVAKEFNGEIISADSMQVYKYMDVGTAKITKEEMQGIPHYLIDIVEPDQEFSVAEYEKRA
KEIIKDIYKRGKLPIIVGGTGLYINSIIYIMHFSDFEGSKEFREKMKELANTYGSQYLYEKLKSVDPEAAKKIHPNDIRR
IIRALEVYEFTGKPISYYQKMSGMRLNPEYQPIMIGLNFRDRQILYDRINQRVDEMIKNNLVEEVVNLLKIGYNKDSTAM
QALGYKEIVEYLKGEISLEEAIEKIKKGTRRYAKRQITWFKGYDFIKWFFVDDYKNYEELKKNIIKYLAGKLNF

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=33.1210191082803, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1790613, Length=304, Percent_Identity=35.8552631578947, Blast_Score=207, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI25144712, Length=310, Percent_Identity=31.6129032258064, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=32.6530612244898, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24649830, Length=329, Percent_Identity=32.2188449848024, Blast_Score=167, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_THEP3 (B0K9L7)

Other databases:

- EMBL:   CP000924
- RefSeq:   YP_001665166.1
- ProteinModelPortal:   B0K9L7
- SMR:   B0K9L7
- GeneID:   5875250
- GenomeReviews:   CP000924_GR
- KEGG:   tpd:Teth39_1176
- HOGENOM:   HBG559459
- OMA:   LPYTVAN
- ProtClustDB:   PRK00091
- BioCyc:   TPSE340099:TETH39_1176-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36759; Mature: 36628

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQIVLIVGPTASGKSKLAVDVAKEFNGEIISADSMQVYKYMDVGTAKITKEEMQGIPH
CEEEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHCCHHHCCHHHHHCCCH
YLIDIVEPDQEFSVAEYEKRAKEIIKDIYKRGKLPIIVGGTGLYINSIIYIMHFSDFEGS
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHEEEEEECCCCCC
KEFREKMKELANTYGSQYLYEKLKSVDPEAAKKIHPNDIRRIIRALEVYEFTGKPISYYQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
KMSGMRLNPEYQPIMIGLNFRDRQILYDRINQRVDEMIKNNLVEEVVNLLKIGYNKDSTA
HHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
MQALGYKEIVEYLKGEISLEEAIEKIKKGTRRYAKRQITWFKGYDFIKWFFVDDYKNYEE
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHH
LKKNIIKYLAGKLNF
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIQIVLIVGPTASGKSKLAVDVAKEFNGEIISADSMQVYKYMDVGTAKITKEEMQGIPH
EEEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHCCHHHCCHHHHHCCCH
YLIDIVEPDQEFSVAEYEKRAKEIIKDIYKRGKLPIIVGGTGLYINSIIYIMHFSDFEGS
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHEEEEEECCCCCC
KEFREKMKELANTYGSQYLYEKLKSVDPEAAKKIHPNDIRRIIRALEVYEFTGKPISYYQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
KMSGMRLNPEYQPIMIGLNFRDRQILYDRINQRVDEMIKNNLVEEVVNLLKIGYNKDSTA
HHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
MQALGYKEIVEYLKGEISLEEAIEKIKKGTRRYAKRQITWFKGYDFIKWFFVDDYKNYEE
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHH
LKKNIIKYLAGKLNF
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA