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The map label for this gene is pycB [H]

Identifier: 167040220

GI number: 167040220

Start: 1601485

End: 1602882

Strand: Direct

Name: pycB [H]

Synonym: Teth514_1582

Alternate gene names: 167040220

Gene position: 1601485-1602882 (Clockwise)

Preceding gene: 167040219

Following gene: 167040221

Centisome position: 65.17

GC content: 34.12

Gene sequence:

>1398_bases
ATGAGTAATAAAATTAAAATTACAGAAACTGTATTAAGAGATGCCCATCAGTCACTCTTGGCTACTCGAATGTCGACGGA
AGAAATGCTCCCAATTGCAGAAAAATTGGATGCAGTAGGATATTATTCTATTGAAGCGTGGGGAGGAGCTACCTTTGATG
CTTGTATGAGATTTCTTGACGAAGACCCTTGGGAAAGATTGAGAAGATTAAAGAAGAGGATAAAAAATACCCCTCTTCAA
ATGCTTTTGAGAGGACAAAATCTATTAGGATATAGGCATTATCCTGATGATATAGTAGAAAAATTTGTTGAGAGGGCTGT
TGCTAACGGAATAGATATAATAAGAATTTTTGATGCTTTAAATGATGTACGAAATCTTGAAGTTGCCGTTAAAGCTACTA
AAAAAGCGGGAGCCCACGCTCAAGGTACCCTTGTTTATACTATAAGTCCTGTTCACAACATTGACCATTATATTAAAGTA
GCAAAAGAGCTGACAGAATTAGGAGTGGATTCCATATGCATAAAGGATATGTCAGGAATATTAACTCCTTATGTTGCTTA
TGAGTTAGTAAAAAGCCTTAAAGATGCAGTAAATATTCCTATACAGTTGCACAGCCATTATACGAGTGGAATGGCGGCTA
TGACTTATCTTAAAGCAATAGAGGCTGGAGTAGATATAATAGACACTGCTATTTCACCTTTGGCATTAGGAACTTCTCAA
CCTGCTACAGAAACAATGGTAGCTATTTTAAAAGGCACTCAATATGACACTGGTTTGGATATGGAGTTATTATCAGAAAT
TGCTTCATACTTTAAAGAAGTAAAACAAAATCACAGTAAAGAAATAGATTTATCTATGGTTATGGGCGTAGATACAGATG
TATTAATTTATCAAGTTCCAGGAGGAATGCTATCAAATTTAATTTCCCAATTAAAACAACAAAATGTTTTAGATAAGTAC
CCAGAGGTTTTAAAAGAAATTCCTAAAGTTAGAGAAGAATTAGGATACCCACCTCTTGTTACTCCTATGAGTCAGATGGT
AGGGACTCAAGCTGTCTTGAATGTAATAACTGGAGAAAGGTATAAAATGGTACCAAAAGAAGTAAAAGATTATGTGAAAG
GACTGTATGGTAGACCACCAGCGCCAATTTCAGAAGAAATAAAGAAAAAAATAATAGGAGATGAAGAGGTAATAGATATT
AGACCAGCAGACCTTTTAAAACCGCAATTTGAAGCGATTAAAGAAGAAATTAAAGAGTATTATGAACAAGAAGAAGATGT
TTTATCTTATGCTCTTTTCCCACAAGTAGCAAAGAAGTTTTTTGAATACAGAAGAGCAAAAAAATATCAAATAGATGCAA
CTCTTCTTAATATGGAATATATGACATATCCAGTTTAA

Upstream 100 bases:

>100_bases
TCTATCCTTTCCTTCTTCTAAAAATTTTGAGCCTTTTGGCTCTTTTTTTATTTTGTTTCTTTTGAAAAAATAGTATAATA
TATTATGAGGAGGTATTAAT

Downstream 100 bases:

>100_bases
CAGGGAAAATTATTTCCCTGTTTTTTTGTGTATATTTAAATTCCTCCTGTAACATATATTTTTATCCTTCATAACATGTA
GTAGAAAGTTTAAAGGAGGG

Product: oxaloacetate decarboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSNKIKITETVLRDAHQSLLATRMSTEEMLPIAEKLDAVGYYSIEAWGGATFDACMRFLDEDPWERLRRLKKRIKNTPLQ
MLLRGQNLLGYRHYPDDIVEKFVERAVANGIDIIRIFDALNDVRNLEVAVKATKKAGAHAQGTLVYTISPVHNIDHYIKV
AKELTELGVDSICIKDMSGILTPYVAYELVKSLKDAVNIPIQLHSHYTSGMAAMTYLKAIEAGVDIIDTAISPLALGTSQ
PATETMVAILKGTQYDTGLDMELLSEIASYFKEVKQNHSKEIDLSMVMGVDTDVLIYQVPGGMLSNLISQLKQQNVLDKY
PEVLKEIPKVREELGYPPLVTPMSQMVGTQAVLNVITGERYKMVPKEVKDYVKGLYGRPPAPISEEIKKKIIGDEEVIDI
RPADLLKPQFEAIKEEIKEYYEQEEDVLSYALFPQVAKKFFEYRRAKKYQIDATLLNMEYMTYPV

Sequences:

>Translated_465_residues
MSNKIKITETVLRDAHQSLLATRMSTEEMLPIAEKLDAVGYYSIEAWGGATFDACMRFLDEDPWERLRRLKKRIKNTPLQ
MLLRGQNLLGYRHYPDDIVEKFVERAVANGIDIIRIFDALNDVRNLEVAVKATKKAGAHAQGTLVYTISPVHNIDHYIKV
AKELTELGVDSICIKDMSGILTPYVAYELVKSLKDAVNIPIQLHSHYTSGMAAMTYLKAIEAGVDIIDTAISPLALGTSQ
PATETMVAILKGTQYDTGLDMELLSEIASYFKEVKQNHSKEIDLSMVMGVDTDVLIYQVPGGMLSNLISQLKQQNVLDKY
PEVLKEIPKVREELGYPPLVTPMSQMVGTQAVLNVITGERYKMVPKEVKDYVKGLYGRPPAPISEEIKKKIIGDEEVIDI
RPADLLKPQFEAIKEEIKEYYEQEEDVLSYALFPQVAKKFFEYRRAKKYQIDATLLNMEYMTYPV
>Mature_464_residues
SNKIKITETVLRDAHQSLLATRMSTEEMLPIAEKLDAVGYYSIEAWGGATFDACMRFLDEDPWERLRRLKKRIKNTPLQM
LLRGQNLLGYRHYPDDIVEKFVERAVANGIDIIRIFDALNDVRNLEVAVKATKKAGAHAQGTLVYTISPVHNIDHYIKVA
KELTELGVDSICIKDMSGILTPYVAYELVKSLKDAVNIPIQLHSHYTSGMAAMTYLKAIEAGVDIIDTAISPLALGTSQP
ATETMVAILKGTQYDTGLDMELLSEIASYFKEVKQNHSKEIDLSMVMGVDTDVLIYQVPGGMLSNLISQLKQQNVLDKYP
EVLKEIPKVREELGYPPLVTPMSQMVGTQAVLNVITGERYKMVPKEVKDYVKGLYGRPPAPISEEIKKKIIGDEEVIDIR
PADLLKPQFEAIKEEIKEYYEQEEDVLSYALFPQVAKKFFEYRRAKKYQIDATLLNMEYMTYPV

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=459, Percent_Identity=34.640522875817, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI106049295, Length=459, Percent_Identity=34.640522875817, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI106049292, Length=459, Percent_Identity=34.640522875817, Blast_Score=274, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17562816, Length=464, Percent_Identity=37.9310344827586, Blast_Score=304, Evalue=7e-83,
Organism=Saccharomyces cerevisiae, GI6319695, Length=464, Percent_Identity=36.4224137931034, Blast_Score=290, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6321376, Length=464, Percent_Identity=35.9913793103448, Blast_Score=289, Evalue=8e-79,
Organism=Drosophila melanogaster, GI24652212, Length=462, Percent_Identity=37.8787878787879, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24652210, Length=462, Percent_Identity=37.8787878787879, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24652214, Length=462, Percent_Identity=37.8787878787879, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI19921944, Length=462, Percent_Identity=37.8787878787879, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24652216, Length=462, Percent_Identity=37.8787878787879, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI281363050, Length=462, Percent_Identity=37.8787878787879, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24652224, Length=462, Percent_Identity=37.8787878787879, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24652222, Length=462, Percent_Identity=37.8787878787879, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24652220, Length=462, Percent_Identity=37.8787878787879, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24652218, Length=462, Percent_Identity=37.8787878787879, Blast_Score=295, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 52582; Mature: 52451

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKIKITETVLRDAHQSLLATRMSTEEMLPIAEKLDAVGYYSIEAWGGATFDACMRFLD
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHC
EDPWERLRRLKKRIKNTPLQMLLRGQNLLGYRHYPDDIVEKFVERAVANGIDIIRIFDAL
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
NDVRNLEVAVKATKKAGAHAQGTLVYTISPVHNIDHYIKVAKELTELGVDSICIKDMSGI
HHHHHHHHHEEEHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LTPYVAYELVKSLKDAVNIPIQLHSHYTSGMAAMTYLKAIEAGVDIIDTAISPLALGTSQ
HHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
PATETMVAILKGTQYDTGLDMELLSEIASYFKEVKQNHSKEIDLSMVMGVDTDVLIYQVP
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCEEEEECC
GGMLSNLISQLKQQNVLDKYPEVLKEIPKVREELGYPPLVTPMSQMVGTQAVLNVITGER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCH
YKMVPKEVKDYVKGLYGRPPAPISEEIKKKIIGDEEVIDIRPADLLKPQFEAIKEEIKEY
HHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHHH
YEQEEDVLSYALFPQVAKKFFEYRRAKKYQIDATLLNMEYMTYPV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEECHHHCCCC
>Mature Secondary Structure 
SNKIKITETVLRDAHQSLLATRMSTEEMLPIAEKLDAVGYYSIEAWGGATFDACMRFLD
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHC
EDPWERLRRLKKRIKNTPLQMLLRGQNLLGYRHYPDDIVEKFVERAVANGIDIIRIFDAL
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
NDVRNLEVAVKATKKAGAHAQGTLVYTISPVHNIDHYIKVAKELTELGVDSICIKDMSGI
HHHHHHHHHEEEHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LTPYVAYELVKSLKDAVNIPIQLHSHYTSGMAAMTYLKAIEAGVDIIDTAISPLALGTSQ
HHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
PATETMVAILKGTQYDTGLDMELLSEIASYFKEVKQNHSKEIDLSMVMGVDTDVLIYQVP
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCEEEEECC
GGMLSNLISQLKQQNVLDKYPEVLKEIPKVREELGYPPLVTPMSQMVGTQAVLNVITGER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCH
YKMVPKEVKDYVKGLYGRPPAPISEEIKKKIIGDEEVIDIRPADLLKPQFEAIKEEIKEY
HHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHHH
YEQEEDVLSYALFPQVAKKFFEYRRAKKYQIDATLLNMEYMTYPV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEECHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]