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The map label for this gene is speE1 [H]

Identifier: 167040218

GI number: 167040218

Start: 1599704

End: 1600537

Strand: Direct

Name: speE1 [H]

Synonym: Teth514_1580

Alternate gene names: 167040218

Gene position: 1599704-1600537 (Clockwise)

Preceding gene: 167040217

Following gene: 167040219

Centisome position: 65.1

GC content: 33.21

Gene sequence:

>834_bases
ATGGAATTATGGTTTACAGAAAATCAGGATGAAAATTTGAGATTTTCTTTGAAAGTTAAAGAAACATTGGTGGTAGAAAA
AACTCCTTATCAACATTTAGCAATACTCGATACATATCAATTTGGAAGAGTATTAACTTTAGATGGTATACTTCAAACCA
CTGAAAAAGATGAATTTGTATATCATGAGATGATAGTACATGTACCCCTCTTTACTCATAAAAATCCAAAAAGCGTATTA
ATTGTTGGAGGCGGAGATGGAGGCTCAGTAAGAGAGGTCTTAAAGCACCCTTCTGTAGAAAGAGTAGTTTTGGCAGAAAT
TGACGAGGCAGTAGTGAGAAATTCTAAAAAATATCTTCCTACAATAAGCCAGGCATTAGATGACCCAAGAGTAGAAATAA
TGATTGGAGATGGAATTAAATATGTTAATGAACACAAAAATGAATTTGATGTGGTAATAGTGGATTCTACTGACCCAATT
GGGCCAGCTGTAGGTCTTTTTACTTCTGATTTTTATAAAGCCGTATATGAATGCTTGAAAGAAGATGGTATTATTGTGGC
TCAGACAGAATCACCTTTTATTTATGGAAAATTGATAAACAAACTCAGCAAAATGTTCAAAGAAATATATCCTATTACAA
AAGCATATATAGCAACAATACCGACTTATCCAGGAAGTTTGTGGACTTTTACCATGGGTTCAAAAAAATATGACCCTGAA
GAAGTGGACATAAATTCTATTCCAAGAATAGATACTAAATACTATACACCTGAAATTCACAAAGCGGCCTTTGTACTGCC
TAAATTTGTGAAGGATATTTTTGATGAGGTGTAA

Upstream 100 bases:

>100_bases
TAAAGAAAAAGGCGATTTTCAGATAGTTAAATTAAGAGAGTTTTTTGGAGAGTGAGATATTTTTTTAAATATATTATTAA
AAATCTTGGAGGTGTTTTCA

Downstream 100 bases:

>100_bases
TATGATAGAAGGAAAATATTTTGTTGATCCGGGAAAGTTTTTAGGGGCTACTGAAGATTATGAAAAGGCAGAGGTAGTTA
TACTAGGTTTGCCTATGGAT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MELWFTENQDENLRFSLKVKETLVVEKTPYQHLAILDTYQFGRVLTLDGILQTTEKDEFVYHEMIVHVPLFTHKNPKSVL
IVGGGDGGSVREVLKHPSVERVVLAEIDEAVVRNSKKYLPTISQALDDPRVEIMIGDGIKYVNEHKNEFDVVIVDSTDPI
GPAVGLFTSDFYKAVYECLKEDGIIVAQTESPFIYGKLINKLSKMFKEIYPITKAYIATIPTYPGSLWTFTMGSKKYDPE
EVDINSIPRIDTKYYTPEIHKAAFVLPKFVKDIFDEV

Sequences:

>Translated_277_residues
MELWFTENQDENLRFSLKVKETLVVEKTPYQHLAILDTYQFGRVLTLDGILQTTEKDEFVYHEMIVHVPLFTHKNPKSVL
IVGGGDGGSVREVLKHPSVERVVLAEIDEAVVRNSKKYLPTISQALDDPRVEIMIGDGIKYVNEHKNEFDVVIVDSTDPI
GPAVGLFTSDFYKAVYECLKEDGIIVAQTESPFIYGKLINKLSKMFKEIYPITKAYIATIPTYPGSLWTFTMGSKKYDPE
EVDINSIPRIDTKYYTPEIHKAAFVLPKFVKDIFDEV
>Mature_277_residues
MELWFTENQDENLRFSLKVKETLVVEKTPYQHLAILDTYQFGRVLTLDGILQTTEKDEFVYHEMIVHVPLFTHKNPKSVL
IVGGGDGGSVREVLKHPSVERVVLAEIDEAVVRNSKKYLPTISQALDDPRVEIMIGDGIKYVNEHKNEFDVVIVDSTDPI
GPAVGLFTSDFYKAVYECLKEDGIIVAQTESPFIYGKLINKLSKMFKEIYPITKAYIATIPTYPGSLWTFTMGSKKYDPE
EVDINSIPRIDTKYYTPEIHKAAFVLPKFVKDIFDEV

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=284, Percent_Identity=38.7323943661972, Blast_Score=233, Evalue=1e-61,
Organism=Escherichia coli, GI1786312, Length=267, Percent_Identity=42.3220973782771, Blast_Score=218, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI25154106, Length=267, Percent_Identity=43.0711610486891, Blast_Score=224, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI212645616, Length=221, Percent_Identity=46.1538461538462, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6325326, Length=282, Percent_Identity=40.7801418439716, Blast_Score=222, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6323175, Length=288, Percent_Identity=37.1527777777778, Blast_Score=198, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24645443, Length=280, Percent_Identity=44.6428571428571, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI21355941, Length=217, Percent_Identity=43.778801843318, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI21358269, Length=169, Percent_Identity=33.1360946745562, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24663238, Length=169, Percent_Identity=33.1360946745562, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 31575; Mature: 31575

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELWFTENQDENLRFSLKVKETLVVEKTPYQHLAILDTYQFGRVLTLDGILQTTEKDEFV
CCEEECCCCCCCEEEEEEEEEEEEEECCCCCEEEEEEHHCCCCEEEECHHHHCCCCCHHH
YHEMIVHVPLFTHKNPKSVLIVGGGDGGSVREVLKHPSVERVVLAEIDEAVVRNSKKYLP
HHHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCC
TISQALDDPRVEIMIGDGIKYVNEHKNEFDVVIVDSTDPIGPAVGLFTSDFYKAVYECLK
HHHHHCCCCCEEEEECCCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
EDGIIVAQTESPFIYGKLINKLSKMFKEIYPITKAYIATIPTYPGSLWTFTMGSKKYDPE
HCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCC
EVDINSIPRIDTKYYTPEIHKAAFVLPKFVKDIFDEV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELWFTENQDENLRFSLKVKETLVVEKTPYQHLAILDTYQFGRVLTLDGILQTTEKDEFV
CCEEECCCCCCCEEEEEEEEEEEEEECCCCCEEEEEEHHCCCCEEEECHHHHCCCCCHHH
YHEMIVHVPLFTHKNPKSVLIVGGGDGGSVREVLKHPSVERVVLAEIDEAVVRNSKKYLP
HHHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCC
TISQALDDPRVEIMIGDGIKYVNEHKNEFDVVIVDSTDPIGPAVGLFTSDFYKAVYECLK
HHHHHCCCCCEEEEECCCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
EDGIIVAQTESPFIYGKLINKLSKMFKEIYPITKAYIATIPTYPGSLWTFTMGSKKYDPE
HCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCC
EVDINSIPRIDTKYYTPEIHKAAFVLPKFVKDIFDEV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]