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The map label for this gene is crp [H]

Identifier: 167040017

GI number: 167040017

Start: 1417368

End: 1418054

Strand: Direct

Name: crp [H]

Synonym: Teth514_1376

Alternate gene names: 167040017

Gene position: 1417368-1418054 (Clockwise)

Preceding gene: 167040016

Following gene: 167040019

Centisome position: 57.68

GC content: 26.64

Gene sequence:

>687_bases
ATGACTATAAGTTCGGTATATAATGAAATGTTTGATATAAATCGACAGAAAGATATGAGAAAATTTTTCCTTAATATAGC
TATACAGGGATATAAAAAGAGTTTTTTGAAAAATGAAACTATAACAATTAATTATAACAAACCATATATTGCCATAATAA
CGAAAGGGTTGATAAAGCAATTTATTTCAAATAAAAATGGAAAGATAAAAGTCCTTTATTTGTTGCAACCAGGGGAAATA
TTTGGAGAATTTTCCTATTTAGGAGGAGGATTTGATTTAATTGAAGGTCAAGCTATAAAAGATAGTGAAATATCTATTAT
TTTTGAAGATAAATTAAGTTCTATTCTGGAAACAAATCCTGATATGTATAAATATATTGCTCATAGCATGAGCAGAAAAT
TTAGAATTGTTACTATGCAGATGAGCGATTTAATTTTCAAAGATTCAATGGGAAGACTGTGCGATTTTCTAATTAGATTA
TTCTATCAACAAGGCAAGAAGATAAAAAAAGGTTATATAATAGATATTCCTTTAACCCATGAGAATATTGCCAATTTAAT
AGGATGTGATAGAGTAACGGTTACAAAAGGACTAAATAAGTTAAAAAAAGAAGGTTTAATTGAGATAGATGGAAAAAAGA
TTATAATAAAAGACCTTCGCTACCTTGAAAAGTATATAGATTCATAA

Upstream 100 bases:

>100_bases
TAAGGTTGACTTTTTTAATTAAACATATTGTAAAAATAGTAAAATTGTTGGTGCAATTTTTATTGTTTCAAACAGGAGAG
AAGTGGGGAGCTGAAAGGGG

Downstream 100 bases:

>100_bases
CATTTAATCGTGAAATAATAAGTTGCGAGCCCCGTCACTTATTAGGGGGTTCGCAATAAAAAGTATAATTATTTAACCGT
TTCAATTATAGCTTTTACAA

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTISSVYNEMFDINRQKDMRKFFLNIAIQGYKKSFLKNETITINYNKPYIAIITKGLIKQFISNKNGKIKVLYLLQPGEI
FGEFSYLGGGFDLIEGQAIKDSEISIIFEDKLSSILETNPDMYKYIAHSMSRKFRIVTMQMSDLIFKDSMGRLCDFLIRL
FYQQGKKIKKGYIIDIPLTHENIANLIGCDRVTVTKGLNKLKKEGLIEIDGKKIIIKDLRYLEKYIDS

Sequences:

>Translated_228_residues
MTISSVYNEMFDINRQKDMRKFFLNIAIQGYKKSFLKNETITINYNKPYIAIITKGLIKQFISNKNGKIKVLYLLQPGEI
FGEFSYLGGGFDLIEGQAIKDSEISIIFEDKLSSILETNPDMYKYIAHSMSRKFRIVTMQMSDLIFKDSMGRLCDFLIRL
FYQQGKKIKKGYIIDIPLTHENIANLIGCDRVTVTKGLNKLKKEGLIEIDGKKIIIKDLRYLEKYIDS
>Mature_227_residues
TISSVYNEMFDINRQKDMRKFFLNIAIQGYKKSFLKNETITINYNKPYIAIITKGLIKQFISNKNGKIKVLYLLQPGEIF
GEFSYLGGGFDLIEGQAIKDSEISIIFEDKLSSILETNPDMYKYIAHSMSRKFRIVTMQMSDLIFKDSMGRLCDFLIRLF
YQQGKKIKKGYIIDIPLTHENIANLIGCDRVTVTKGLNKLKKEGLIEIDGKKIIIKDLRYLEKYIDS

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=175, Percent_Identity=28, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26432; Mature: 26300

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISSVYNEMFDINRQKDMRKFFLNIAIQGYKKSFLKNETITINYNKPYIAIITKGLIKQ
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHH
FISNKNGKIKVLYLLQPGEIFGEFSYLGGGFDLIEGQAIKDSEISIIFEDKLSSILETNP
HHCCCCCCEEEEEEECCHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEHHHHHHHHHCCC
DMYKYIAHSMSRKFRIVTMQMSDLIFKDSMGRLCDFLIRLFYQQGKKIKKGYIIDIPLTH
HHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCH
ENIANLIGCDRVTVTKGLNKLKKEGLIEIDGKKIIIKDLRYLEKYIDS
HHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
TISSVYNEMFDINRQKDMRKFFLNIAIQGYKKSFLKNETITINYNKPYIAIITKGLIKQ
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHH
FISNKNGKIKVLYLLQPGEIFGEFSYLGGGFDLIEGQAIKDSEISIIFEDKLSSILETNP
HHCCCCCCEEEEEEECCHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEHHHHHHHHHCCC
DMYKYIAHSMSRKFRIVTMQMSDLIFKDSMGRLCDFLIRLFYQQGKKIKKGYIIDIPLTH
HHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCH
ENIANLIGCDRVTVTKGLNKLKKEGLIEIDGKKIIIKDLRYLEKYIDS
HHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]