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The map label for this gene is pckA [H]

Identifier: 167039929

GI number: 167039929

Start: 1323124

End: 1323825

Strand: Reverse

Name: pckA [H]

Synonym: Teth514_1285

Alternate gene names: 167039929

Gene position: 1323825-1323124 (Counterclockwise)

Preceding gene: 167039931

Following gene: 167039874

Centisome position: 53.87

GC content: 34.62

Gene sequence:

>702_bases
ATGTACGGTCTTGAAAAATTAAATATAATCAATGTTAAAAACGTTTACCGTAATCTTCCCGTCCCTAAATTAGTTGAAGA
AGCTATAAAAAGAGGAGAAGGAGTACTTGCAAATAATGGTGCTTTTAATGTTTATACCGGTAAATACACAGGTCGTTCTC
CAAATGATAAATTTATTGTGGATGAACCAGAAGTTCATGAAGATATATGGTGGGAAAATAATAAAGCTATTTCTATGGAT
AAATTTGAAAGGTTGTACAACCGCTTAACTGCTTATTTACAAAATCGAGACATTTTTATATTTGACGGTTTTGTAGGTGC
TGACCTTCAATATAGAATTCCTATAAGAGTTATAACAGAATATGCGTATCAGAGTTTACTGGCACGCCAATTGTTCATTC
GCCCTAATGAAGAAGAACTTAGAAATCACATCCCCGAATACACGCTAATTGCTGCACCAAAGTTCAACTCTATACCTGAA
TTAGACGGTGTAAATTCTGAAGCTTTTATTATATTAAGTTTCACTAAAAAACTAATTATAATCGGTGGCTCTCAGTATGG
AGGGGAAATCAAAAAGTCAATCTTTACCCTCATGAACTATCTGATGCCAAAGCGCGGAGTTTTATCTATGCACTGTTCTG
CAAATATGGGCAAAGATGGAGATACCACTCTCTTTTTCGGAAAAATACAAAGGTGTAACTGA

Upstream 100 bases:

>100_bases
TATTGTAAATGTTAATTTTAAACTTTATAAGATATTTTTGCAATTTTAAACACATTAAAATGCAAATTAGCTTTTATAAA
ATTAAAAGGAGGCATATTAA

Downstream 100 bases:

>100_bases
TGATATCAAAAAATCAGGCCCAAATGTTTAAAAACATAAAGTCTCCTTGCAGTGCGTACTGCTAGGAGACTTTTATAATT
TGTAGCCATTATAATATATC

Product: phosphoenolpyruvate carboxykinase (ATP)

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MYGLEKLNIINVKNVYRNLPVPKLVEEAIKRGEGVLANNGAFNVYTGKYTGRSPNDKFIVDEPEVHEDIWWENNKAISMD
KFERLYNRLTAYLQNRDIFIFDGFVGADLQYRIPIRVITEYAYQSLLARQLFIRPNEEELRNHIPEYTLIAAPKFNSIPE
LDGVNSEAFIILSFTKKLIIIGGSQYGGEIKKSIFTLMNYLMPKRGVLSMHCSANMGKDGDTTLFFGKIQRCN

Sequences:

>Translated_233_residues
MYGLEKLNIINVKNVYRNLPVPKLVEEAIKRGEGVLANNGAFNVYTGKYTGRSPNDKFIVDEPEVHEDIWWENNKAISMD
KFERLYNRLTAYLQNRDIFIFDGFVGADLQYRIPIRVITEYAYQSLLARQLFIRPNEEELRNHIPEYTLIAAPKFNSIPE
LDGVNSEAFIILSFTKKLIIIGGSQYGGEIKKSIFTLMNYLMPKRGVLSMHCSANMGKDGDTTLFFGKIQRCN
>Mature_233_residues
MYGLEKLNIINVKNVYRNLPVPKLVEEAIKRGEGVLANNGAFNVYTGKYTGRSPNDKFIVDEPEVHEDIWWENNKAISMD
KFERLYNRLTAYLQNRDIFIFDGFVGADLQYRIPIRVITEYAYQSLLARQLFIRPNEEELRNHIPEYTLIAAPKFNSIPE
LDGVNSEAFIILSFTKKLIIIGGSQYGGEIKKSIFTLMNYLMPKRGVLSMHCSANMGKDGDTTLFFGKIQRCN

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=237, Percent_Identity=37.9746835443038, Blast_Score=174, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6322950, Length=221, Percent_Identity=41.6289592760181, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 26774; Mature: 26774

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGLEKLNIINVKNVYRNLPVPKLVEEAIKRGEGVLANNGAFNVYTGKYTGRSPNDKFIV
CCCCCEEEEEEHHHHHHCCCCHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCCEEE
DEPEVHEDIWWENNKAISMDKFERLYNRLTAYLQNRDIFIFDGFVGADLQYRIPIRVITE
CCCCCCHHHCCCCCCEECHHHHHHHHHHHHHHHCCCCEEEECCEECCCEEEECHHHHHHH
YAYQSLLARQLFIRPNEEELRNHIPEYTLIAAPKFNSIPELDGVNSEAFIILSFTKKLII
HHHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEE
IGGSQYGGEIKKSIFTLMNYLMPKRGVLSMHCSANMGKDGDTTLFFGKIQRCN
EECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MYGLEKLNIINVKNVYRNLPVPKLVEEAIKRGEGVLANNGAFNVYTGKYTGRSPNDKFIV
CCCCCEEEEEEHHHHHHCCCCHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCCEEE
DEPEVHEDIWWENNKAISMDKFERLYNRLTAYLQNRDIFIFDGFVGADLQYRIPIRVITE
CCCCCCHHHCCCCCCEECHHHHHHHHHHHHHHHCCCCEEEECCEECCCEEEECHHHHHHH
YAYQSLLARQLFIRPNEEELRNHIPEYTLIAAPKFNSIPELDGVNSEAFIILSFTKKLII
HHHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEE
IGGSQYGGEIKKSIFTLMNYLMPKRGVLSMHCSANMGKDGDTTLFFGKIQRCN
EECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]