Click here to switch to the map view.

The map label for this gene is metK [H]

Identifier: 167039888

GI number: 167039888

Start: 1284142

End: 1285317

Strand: Direct

Name: metK [H]

Synonym: Teth514_1243

Alternate gene names: 167039888

Gene position: 1284142-1285317 (Clockwise)

Preceding gene: 167039887

Following gene: 167039889

Centisome position: 52.26

GC content: 39.12

Gene sequence:

>1176_bases
ATGAGTAAGAGATATTTAACAGCAGAAAGTGTATGTGCAGGGCATCCTGATAAACTGTGCGATATTATTGCTGACAGTAT
TTTGGATGCATGTCTTAGAAAAGATAAAGCATCACGCGTAGCTTGTGAGGTTATGGCTACTAAAGGAAAAATTATCGTGG
CGGGCGAGATCTCCTGCAGCGAGAAAATTGATATCAGAAATATTGTTAAGAATGTGCTTAAAGAACTAGGTTATAACCCT
TTGAAATTTTTAATTTATGTATATGTACACAATCAGAGTTCTGATATTGCATCTGGGGTGAATACTGCACTAGAAGCACG
AAATGGTATAAACGAACAATATGGTTCTATCGGTGCTGGTGATCAAGGGACTATGTATGGCTATGCCACTAAAGAAACTA
GAGAAATGCTTCCTCTTCCCCTTGTCTTATCTCATCGAATTGTAAAGAGACTAGATGAGGCAAGAAAAGGTAAGCTGATT
AAAGGTATCCTTCCCGATGGAAAAGCACAGGTAACGATTGAGTATGATGATGATGTTCCAGTGAGAGTTAAAACCATTGT
AGTGTCAGTTCAGCATGAAAAGAATAAAACTCAGGAAGAGCTAAAATCCGATATTTTAAATAATGTGTTATGGCAGTGTT
TTGAGGATTTTCCTTTTGATGATGAAACAGAAATTCTTATCAATCCATCTGGCCAGTTTGTATTAGGTGGACCTGCTGCA
GATACAGGTTTAACTGGAAGAAAGATCATGGTGGACACTTATGGCGGACTTGCATCACATGGTGGCGGAGCACTATGCGG
GAAAGATCCAACCAAAGTAGACCGAAGCGGTGCTTACATGGCTAGGTATATTGCTAAACATATCGTGTGGTGTGATTTGG
CAGAGAAATGTGAAGTGGCTATTTCCTATGCTATTGGCAAGGCAAATCCTGTGGCTTTTTCCATAAATACTTTTGGAACA
GGAACAGTTTCTGATGAAGTTCTAACCATTGCTGCTCAAGAAGTTTTTAATTTGAGGCCTGCAGCAATTATTGAAAAGCT
GCGACTTAGAAACATTCATTACTCCGATACAGCAGTCTATGGTCACTTTAACAGTTGCCTGTTCCCTTGGGAGGATGTTA
ATAAATATAGCGAACTAAAAGAGGCGGTGGGAAAATATGCAGATAGAGAAAATTAA

Upstream 100 bases:

>100_bases
AAAACTGTGCGACAGAGTATTCCGGTACAAATCCACAGGATGATGTGATGGAACGACTGTTGACTGCCCGTAGAGGCAAA
TAATGATAAGGAGATGTGAA

Downstream 100 bases:

>100_bases
GACGAAACTGCTGATCCCTGCTGATTATAATCCTAGAAAGGACTTAAAGCCGGGTGATCCAGAGTATGAAAAGCTAAAAC
GCTCCCTTGAGGAGTTTGGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSKRYLTAESVCAGHPDKLCDIIADSILDACLRKDKASRVACEVMATKGKIIVAGEISCSEKIDIRNIVKNVLKELGYNP
LKFLIYVYVHNQSSDIASGVNTALEARNGINEQYGSIGAGDQGTMYGYATKETREMLPLPLVLSHRIVKRLDEARKGKLI
KGILPDGKAQVTIEYDDDVPVRVKTIVVSVQHEKNKTQEELKSDILNNVLWQCFEDFPFDDETEILINPSGQFVLGGPAA
DTGLTGRKIMVDTYGGLASHGGGALCGKDPTKVDRSGAYMARYIAKHIVWCDLAEKCEVAISYAIGKANPVAFSINTFGT
GTVSDEVLTIAAQEVFNLRPAAIIEKLRLRNIHYSDTAVYGHFNSCLFPWEDVNKYSELKEAVGKYADREN

Sequences:

>Translated_391_residues
MSKRYLTAESVCAGHPDKLCDIIADSILDACLRKDKASRVACEVMATKGKIIVAGEISCSEKIDIRNIVKNVLKELGYNP
LKFLIYVYVHNQSSDIASGVNTALEARNGINEQYGSIGAGDQGTMYGYATKETREMLPLPLVLSHRIVKRLDEARKGKLI
KGILPDGKAQVTIEYDDDVPVRVKTIVVSVQHEKNKTQEELKSDILNNVLWQCFEDFPFDDETEILINPSGQFVLGGPAA
DTGLTGRKIMVDTYGGLASHGGGALCGKDPTKVDRSGAYMARYIAKHIVWCDLAEKCEVAISYAIGKANPVAFSINTFGT
GTVSDEVLTIAAQEVFNLRPAAIIEKLRLRNIHYSDTAVYGHFNSCLFPWEDVNKYSELKEAVGKYADREN
>Mature_390_residues
SKRYLTAESVCAGHPDKLCDIIADSILDACLRKDKASRVACEVMATKGKIIVAGEISCSEKIDIRNIVKNVLKELGYNPL
KFLIYVYVHNQSSDIASGVNTALEARNGINEQYGSIGAGDQGTMYGYATKETREMLPLPLVLSHRIVKRLDEARKGKLIK
GILPDGKAQVTIEYDDDVPVRVKTIVVSVQHEKNKTQEELKSDILNNVLWQCFEDFPFDDETEILINPSGQFVLGGPAAD
TGLTGRKIMVDTYGGLASHGGGALCGKDPTKVDRSGAYMARYIAKHIVWCDLAEKCEVAISYAIGKANPVAFSINTFGTG
TVSDEVLTIAAQEVFNLRPAAIIEKLRLRNIHYSDTAVYGHFNSCLFPWEDVNKYSELKEAVGKYADREN

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=377, Percent_Identity=42.1750663129974, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI5174529, Length=377, Percent_Identity=43.5013262599469, Blast_Score=290, Evalue=2e-78,
Organism=Escherichia coli, GI1789311, Length=391, Percent_Identity=44.7570332480818, Blast_Score=346, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17538498, Length=380, Percent_Identity=43.9473684210526, Blast_Score=303, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI17551082, Length=383, Percent_Identity=43.3420365535248, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI25145633, Length=385, Percent_Identity=43.3766233766234, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17538494, Length=380, Percent_Identity=43.421052631579, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI32565905, Length=373, Percent_Identity=40.2144772117962, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI32565911, Length=332, Percent_Identity=43.0722891566265, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI25145635, Length=336, Percent_Identity=42.5595238095238, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI32565903, Length=291, Percent_Identity=43.6426116838488, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI32565909, Length=77, Percent_Identity=45.4545454545455, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323209, Length=377, Percent_Identity=44.2970822281167, Blast_Score=303, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6320710, Length=386, Percent_Identity=42.4870466321244, Blast_Score=296, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24580527, Length=378, Percent_Identity=43.6507936507937, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24580525, Length=378, Percent_Identity=43.6507936507937, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI45552159, Length=378, Percent_Identity=43.1216931216931, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24580529, Length=378, Percent_Identity=43.1216931216931, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24580537, Length=378, Percent_Identity=43.1216931216931, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24580533, Length=378, Percent_Identity=43.1216931216931, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24580535, Length=378, Percent_Identity=43.1216931216931, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24580531, Length=378, Percent_Identity=43.1216931216931, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24580541, Length=367, Percent_Identity=43.0517711171662, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI24580539, Length=124, Percent_Identity=39.5161290322581, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43006; Mature: 42874

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00376 ADOMET_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRYLTAESVCAGHPDKLCDIIADSILDACLRKDKASRVACEVMATKGKIIVAGEISCS
CCCCEECHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCEEEEECCCCC
EKIDIRNIVKNVLKELGYNPLKFLIYVYVHNQSSDIASGVNTALEARNGINEQYGSIGAG
CCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHCCCCCC
DQGTMYGYATKETREMLPLPLVLSHRIVKRLDEARKGKLIKGILPDGKAQVTIEYDDDVP
CCCCEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCCCC
VRVKTIVVSVQHEKNKTQEELKSDILNNVLWQCFEDFPFDDETEILINPSGQFVLGGPAA
EEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCC
DTGLTGRKIMVDTYGGLASHGGGALCGKDPTKVDRSGAYMARYIAKHIVWCDLAEKCEVA
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHEE
ISYAIGKANPVAFSINTFGTGTVSDEVLTIAAQEVFNLRPAAIIEKLRLRNIHYSDTAVY
EEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEE
GHFNSCLFPWEDVNKYSELKEAVGKYADREN
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKRYLTAESVCAGHPDKLCDIIADSILDACLRKDKASRVACEVMATKGKIIVAGEISCS
CCCEECHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCEEEEECCCCC
EKIDIRNIVKNVLKELGYNPLKFLIYVYVHNQSSDIASGVNTALEARNGINEQYGSIGAG
CCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHCCCCCC
DQGTMYGYATKETREMLPLPLVLSHRIVKRLDEARKGKLIKGILPDGKAQVTIEYDDDVP
CCCCEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCCCC
VRVKTIVVSVQHEKNKTQEELKSDILNNVLWQCFEDFPFDDETEILINPSGQFVLGGPAA
EEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCC
DTGLTGRKIMVDTYGGLASHGGGALCGKDPTKVDRSGAYMARYIAKHIVWCDLAEKCEVA
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHEE
ISYAIGKANPVAFSINTFGTGTVSDEVLTIAAQEVFNLRPAAIIEKLRLRNIHYSDTAVY
EEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEE
GHFNSCLFPWEDVNKYSELKEAVGKYADREN
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]