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The map label for this gene is hpaIM [H]

Identifier: 167039889

GI number: 167039889

Start: 1285298

End: 1286536

Strand: Direct

Name: hpaIM [H]

Synonym: Teth514_1244

Alternate gene names: 167039889

Gene position: 1285298-1286536 (Clockwise)

Preceding gene: 167039888

Following gene: 167039890

Centisome position: 52.31

GC content: 41.0

Gene sequence:

>1239_bases
ATGCAGATAGAGAAAATTAAGACGAAACTGCTGATCCCTGCTGATTATAATCCTAGAAAGGACTTAAAGCCGGGTGATCC
AGAGTATGAAAAGCTAAAACGCTCCCTTGAGGAGTTTGGCTACGTCGAACCAGTTATTTGGAATAGAACCACAGGTAGAG
TAGTTGGTGGCCATCAGCGATTGAAAATCCTACTGAACATGGGTATGGAGGAAGTAGATTGCGTAGTTGTTGAGATGGAT
GAGGAAAAGGAAAAAGCCCTCAATATTGCATTGAATAAAATAAACGGAGATTGGGATAAGGAAAAACTGGCACTTCTCAT
CACAGACTTAAACGCCGCAGACTTTGATGTATCTCTCACAGGTTTTGACCCCGGAGAGCTGGATGATCTTTTCAAGGATA
CGTTGAAAGACAAAATAAGAGAAGATGATTTCGATGTGGACAGCGAGCTGAGTAAGCCCGCTGTTTCGCGTTTAGGAGAT
GTATGGATACTTGGTAAGCACAGACTGGTATGTGGAGACAGCACAAAAAAAGAAACATATAACATTTTAATGGAAGGAAA
GGTCGCCAATCTGGTGGTAACTGATCCCCCATACAATGTCAACTATGAAGGAACCGCTGGGAAAATCAAAAATGATAATA
TGGCCAATGACGCATTTTATCAATTTTTATTAGATGCCTTTAAGAATATCGAATCGGTATTGGCATCAGATGGAAGTATA
TATGTTTTCCATGCTGATACAGAAGGACTTAATTTTAGAAAAGCCTTTGTTGACGCAGGCTTTTATCTTTCCGGTACTTG
TATTTGGAAGAAACAGTCATTGGTTCTTGGAAGATCCCCTTATCAGTGGCAGCATGAACCGGTGCTATTCGGTTGGAAAA
AGAAGGGGAAGCATCTCTGGTATTCAGACCGTAAGCAATCAACCATTTGGGAATTTGATAAGCCGAAAAAGAATGCAGAT
CATCCGACTATGAAGCCCATTGCTTTAATTGCCTACCCCATTATGAATTCTAGCCTTACTAATAGTATCGTACTTGATCC
TTTTGGAGGTTCTGGCTCGACGCTGATTGCCTGTGAACAGACCGATAGAATTTGCTATACCATCGAGCTGGATGAAAAGT
ACTGCGATGTTATTGTGAAGCGTTACATTGAGCAGGTTGGAACAGATAAAGATGTCTATGTTATTCGTGAAAAGAGAAGA
TTCCATTTAACGTGGCAGCCACATCCACTGATGAATTAG

Upstream 100 bases:

>100_bases
CATTACTCCGATACAGCAGTCTATGGTCACTTTAACAGTTGCCTGTTCCCTTGGGAGGATGTTAATAAATATAGCGAACT
AAAAGAGGCGGTGGGAAAAT

Downstream 100 bases:

>100_bases
ATTGATAGAGCAAGTTTTAAATGCATTTTTGCACAGAAATAACTTGCTATTGTGTAGCGTTAGAGTGATATATGGTACTA
CCAAATAAGAAAGGGTGCGC

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQIEKIKTKLLIPADYNPRKDLKPGDPEYEKLKRSLEEFGYVEPVIWNRTTGRVVGGHQRLKILLNMGMEEVDCVVVEMD
EEKEKALNIALNKINGDWDKEKLALLITDLNAADFDVSLTGFDPGELDDLFKDTLKDKIREDDFDVDSELSKPAVSRLGD
VWILGKHRLVCGDSTKKETYNILMEGKVANLVVTDPPYNVNYEGTAGKIKNDNMANDAFYQFLLDAFKNIESVLASDGSI
YVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLVLGRSPYQWQHEPVLFGWKKKGKHLWYSDRKQSTIWEFDKPKKNAD
HPTMKPIALIAYPIMNSSLTNSIVLDPFGGSGSTLIACEQTDRICYTIELDEKYCDVIVKRYIEQVGTDKDVYVIREKRR
FHLTWQPHPLMN

Sequences:

>Translated_412_residues
MQIEKIKTKLLIPADYNPRKDLKPGDPEYEKLKRSLEEFGYVEPVIWNRTTGRVVGGHQRLKILLNMGMEEVDCVVVEMD
EEKEKALNIALNKINGDWDKEKLALLITDLNAADFDVSLTGFDPGELDDLFKDTLKDKIREDDFDVDSELSKPAVSRLGD
VWILGKHRLVCGDSTKKETYNILMEGKVANLVVTDPPYNVNYEGTAGKIKNDNMANDAFYQFLLDAFKNIESVLASDGSI
YVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLVLGRSPYQWQHEPVLFGWKKKGKHLWYSDRKQSTIWEFDKPKKNAD
HPTMKPIALIAYPIMNSSLTNSIVLDPFGGSGSTLIACEQTDRICYTIELDEKYCDVIVKRYIEQVGTDKDVYVIREKRR
FHLTWQPHPLMN
>Mature_412_residues
MQIEKIKTKLLIPADYNPRKDLKPGDPEYEKLKRSLEEFGYVEPVIWNRTTGRVVGGHQRLKILLNMGMEEVDCVVVEMD
EEKEKALNIALNKINGDWDKEKLALLITDLNAADFDVSLTGFDPGELDDLFKDTLKDKIREDDFDVDSELSKPAVSRLGD
VWILGKHRLVCGDSTKKETYNILMEGKVANLVVTDPPYNVNYEGTAGKIKNDNMANDAFYQFLLDAFKNIESVLASDGSI
YVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLVLGRSPYQWQHEPVLFGWKKKGKHLWYSDRKQSTIWEFDKPKKNAD
HPTMKPIALIAYPIMNSSLTNSIVLDPFGGSGSTLIACEQTDRICYTIELDEKYCDVIVKRYIEQVGTDKDVYVIREKRR
FHLTWQPHPLMN

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 47193; Mature: 47193

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIEKIKTKLLIPADYNPRKDLKPGDPEYEKLKRSLEEFGYVEPVIWNRTTGRVVGGHQR
CCCHHEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCEECCHHH
LKILLNMGMEEVDCVVVEMDEEKEKALNIALNKINGDWDKEKLALLITDLNAADFDVSLT
HHHHHHCCCCCEEEEEEEECCHHHHHHEEEEEECCCCCCCCEEEEEEEECCCCCEEEEEE
GFDPGELDDLFKDTLKDKIREDDFDVDSELSKPAVSRLGDVWILGKHRLVCGDSTKKETY
CCCCCHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHCCCEEEEECEEEEECCCCCCCEE
NILMEGKVANLVVTDPPYNVNYEGTAGKIKNDNMANDAFYQFLLDAFKNIESVLASDGSI
EEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
YVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLVLGRSPYQWQHEPVLFGWKKKGKHLW
EEEECCCCCCCHHHHHHCCCCEEEEEEEEEECCEEECCCCCCCCCCCEEEEECCCCCCCC
YSDRKQSTIWEFDKPKKNADHPTMKPIALIAYPIMNSSLTNSIVLDPFGGSGSTLIACEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCEEEECCCCCCCEEEEEEC
TDRICYTIELDEKYCDVIVKRYIEQVGTDKDVYVIREKRRFHLTWQPHPLMN
CCCEEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCC
>Mature Secondary Structure
MQIEKIKTKLLIPADYNPRKDLKPGDPEYEKLKRSLEEFGYVEPVIWNRTTGRVVGGHQR
CCCHHEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCEECCHHH
LKILLNMGMEEVDCVVVEMDEEKEKALNIALNKINGDWDKEKLALLITDLNAADFDVSLT
HHHHHHCCCCCEEEEEEEECCHHHHHHEEEEEECCCCCCCCEEEEEEEECCCCCEEEEEE
GFDPGELDDLFKDTLKDKIREDDFDVDSELSKPAVSRLGDVWILGKHRLVCGDSTKKETY
CCCCCHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHCCCEEEEECEEEEECCCCCCCEE
NILMEGKVANLVVTDPPYNVNYEGTAGKIKNDNMANDAFYQFLLDAFKNIESVLASDGSI
EEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
YVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLVLGRSPYQWQHEPVLFGWKKKGKHLW
EEEECCCCCCCHHHHHHCCCCEEEEEEEEEECCEEECCCCCCCCCCCEEEEECCCCCCCC
YSDRKQSTIWEFDKPKKNADHPTMKPIALIAYPIMNSSLTNSIVLDPFGGSGSTLIACEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCEEEECCCCCCCEEEEEEC
TDRICYTIELDEKYCDVIVKRYIEQVGTDKDVYVIREKRRFHLTWQPHPLMN
CCCEEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]